9031399

6-amino-1-(pyridin-4-ylmethyl)pyrimidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2393
Screen concentration 185.9 μM
Source Chembridge (Fragment library)
PubChem CID 1526623
SMILES C1=CN=CC=C1CN2C(=CC(=O)NC2=O)N
Standardized SMILES NC1=CC(=O)NC(=O)N1Cc2ccncc2
Molecular weight 218.212
ALogP -0.59
H-bond donor count 2
H-bond acceptor count 4
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.69
% growth inhibition (Hom. pool) 3.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1526623
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:5.09|P-value:1.82E-7|Clearance:0.43||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ACC1(YNR016C)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.11||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:BRN1(YBL097W)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DCP2(YNL118C)|FD-Score:-3.33|P-value:4.35E-4|Clearance:0||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:DRS1(YLL008W)|FD-Score:3.94|P-value:4.02E-5|Clearance:0.04||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:LAS1(YKR063C)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MCD1(YDL003W)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MCM7(YBR202W)|FD-Score:3.85|P-value:5.83E-5|Clearance:0.06||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MED6(YHR058C)|FD-Score:3.99|P-value:3.29E-5|Clearance:0.05||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:NDD1(YOR372C)|FD-Score:-8.78|P-value:8.42E-19|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:PTR3(YFR029W)|FD-Score:3.73|P-value:9.41E-5|Clearance:0.14||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RER2(YBR002C)|FD-Score:5.34|P-value:4.65E-8|Clearance:0.25||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC4(YOL094C)|FD-Score:3.9|P-value:4.79E-5|Clearance:0.03||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RLP7(YNL002C)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.13||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPB8(YOR224C)|FD-Score:5.66|P-value:7.62E-9|Clearance:0.32||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:12.6|P-value:5.68E-37|Clearance:6.15||SGD DESC:RNA polymerase III subunit C53 Gene:RPL15A(YLR029C)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL33A(YPL143W)|FD-Score:4.61|P-value:2.05E-6|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPS3(YNL178W)|FD-Score:-4.28|P-value:9.15E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP45(YDR280W)|FD-Score:4.47|P-value:3.94E-6|Clearance:0.48||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC27(YGL137W)|FD-Score:3.87|P-value:5.44E-5|Clearance:0.02||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SKI6(YGR195W)|FD-Score:3.19|P-value:7.17E-4|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SLY1(YDR189W)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.03||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:TCP1(YDR212W)|FD-Score:3.79|P-value:7.53E-5|Clearance:0.06||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFC1(YBR123C)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.17||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TID3(YIL144W)|FD-Score:-3.34|P-value:4.23E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YDL152W(YDL152W_d)|FD-Score:6.5|P-value:4.11E-11|Clearance:0.84||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR187C(YDR187C_d)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YPL142C(YPL142C_d)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:ABF1(YKL112W)|FD-Score:5.09|P-value:1.82E-7|Clearance:0.43||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ACC1(YNR016C)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.11||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:BRN1(YBL097W)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DCP2(YNL118C)|FD-Score:-3.33|P-value:4.35E-4|Clearance:0||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:DRS1(YLL008W)|FD-Score:3.94|P-value:4.02E-5|Clearance:0.04||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:LAS1(YKR063C)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MCD1(YDL003W)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MCM7(YBR202W)|FD-Score:3.85|P-value:5.83E-5|Clearance:0.06||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MED6(YHR058C)|FD-Score:3.99|P-value:3.29E-5|Clearance:0.05||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:NDD1(YOR372C)|FD-Score:-8.78|P-value:8.42E-19|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:PTR3(YFR029W)|FD-Score:3.73|P-value:9.41E-5|Clearance:0.14||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RER2(YBR002C)|FD-Score:5.34|P-value:4.65E-8|Clearance:0.25||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC4(YOL094C)|FD-Score:3.9|P-value:4.79E-5|Clearance:0.03||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RLP7(YNL002C)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.13||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPB8(YOR224C)|FD-Score:5.66|P-value:7.62E-9|Clearance:0.32||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:12.6|P-value:5.68E-37|Clearance:6.15||SGD DESC:RNA polymerase III subunit C53 Gene:RPL15A(YLR029C)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL33A(YPL143W)|FD-Score:4.61|P-value:2.05E-6|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPS3(YNL178W)|FD-Score:-4.28|P-value:9.15E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP45(YDR280W)|FD-Score:4.47|P-value:3.94E-6|Clearance:0.48||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC27(YGL137W)|FD-Score:3.87|P-value:5.44E-5|Clearance:0.02||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SKI6(YGR195W)|FD-Score:3.19|P-value:7.17E-4|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SLY1(YDR189W)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.03||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:TCP1(YDR212W)|FD-Score:3.79|P-value:7.53E-5|Clearance:0.06||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFC1(YBR123C)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.17||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TID3(YIL144W)|FD-Score:-3.34|P-value:4.23E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YDL152W(YDL152W_d)|FD-Score:6.5|P-value:4.11E-11|Clearance:0.84||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR187C(YDR187C_d)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YPL142C(YPL142C_d)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1526623
Download HOP data (tab-delimited text)  (excel)
Gene:AIM26(YKL037W)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:APJ1(YNL077W)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARG82(YDR173C)|FD-Score:6.05|P-value:7.26E-10||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARL3(YPL051W)|FD-Score:-4.31|P-value:8.21E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATS1(YAL020C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BDH2(YAL061W_p)|FD-Score:10.4|P-value:8.37E-26||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:BUD30(YDL151C_d)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CDC73(YLR418C)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG5(YNL051W)|FD-Score:-3.86|P-value:5.63E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ8(YGL119W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:COX15(YER141W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:CTF19(YPL018W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CTF8(YHR191C)|FD-Score:-4.28|P-value:9.17E-6||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:CTR1(YPR124W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CTR9(YOL145C)|FD-Score:5.34|P-value:4.71E-8||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CYC1(YJR048W)|FD-Score:5.51|P-value:1.82E-8||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:DOC1(YGL240W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:FET4(YMR319C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE4(YNR060W)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FUN14(YAL008W)|FD-Score:5.88|P-value:2.06E-9||SGD DESC:Mitochondrial protein of unknown function Gene:FUR4(YBR021W)|FD-Score:27.9|P-value:3.00E-171||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GLG2(YJL137C)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GND1(YHR183W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GTR2(YGR163W)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUF1(YLR289W)|FD-Score:4.28|P-value:9.42E-6||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HOS2(YGL194C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HPC2(YBR215W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:ICY2(YPL250C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:INO1(YJL153C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IRC21(YMR073C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LDB16(YCL005W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LRO1(YNR008W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LSM7(YNL147W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDM36(YPR083W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MDY2(YOL111C)|FD-Score:-4.88|P-value:5.19E-7||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MRP8(YKL142W)|FD-Score:-4.78|P-value:8.94E-7||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MRPS8(YMR158W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSN2(YMR037C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MTC5(YDR128W)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NMA111(YNL123W)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NOP16(YER002W)|FD-Score:5.26|P-value:7.33E-8||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:ORT1(YOR130C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:OSW2(YLR054C)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PAD1(YDR538W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PBP1(YGR178C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PEX27(YOR193W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PGC1(YPL206C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PIR3(YKL163W)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PMT2(YAL023C)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PRM5(YIL117C)|FD-Score:-3.92|P-value:4.40E-5||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PTK2(YJR059W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTP2(YOR208W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RAM1(YDL090C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RBG1(YAL036C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:REH1(YLR387C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIF1(YBR275C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPN4(YDL020C)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS19A(YOL121C)|FD-Score:-3.12|P-value:9.00E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RTT103(YDR289C)|FD-Score:3.85|P-value:5.88E-5||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAT4(YCR008W)|FD-Score:4.35|P-value:6.87E-6||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SBA1(YKL117W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress Gene:SCD6(YPR129W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SIT4(YDL047W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKN7(YHR206W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNF7(YLR025W)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPF1(YEL031W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSH1(YBR283C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SSU1(YPL092W)|FD-Score:-6.76|P-value:6.72E-12||SGD DESC:Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein Gene:STF1(YDL130W-A)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SVS1(YPL163C)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:4.9|P-value:4.77E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TOS8(YGL096W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication Gene:TRM10(YOL093W)|FD-Score:-5.59|P-value:1.14E-8||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:UBP13(YBL067C)|FD-Score:5.01|P-value:2.78E-7||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UTH1(YKR042W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VBA4(YDR119W_p)|FD-Score:3.8|P-value:7.09E-5||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VPS4(YPR173C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS61(YDR136C_d)|FD-Score:-4.66|P-value:1.58E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAP3(YHL009C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR174C(YBR174C_d)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR238C(YBR238C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR259W(YBR259W_p)|FD-Score:4.85|P-value:6.27E-7||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCL002C(YCL002C_p)|FD-Score:4.31|P-value:8.11E-6||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR087C-A(YCR087C-A_p)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL027C(YDL027C_p)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDL183C(YDL183C)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR278C(YDR278C_d)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YEL045C(YEL045C_d)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER119C-A(YER119C-A_d)|FD-Score:7.44|P-value:5.17E-14||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER156C(YER156C_p)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YFL012W(YFL012W_p)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGR111W(YGR111W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YIR021W-A(YIR021W-A_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YLR326W(YLR326W_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YMD8(YML038C)|FD-Score:-6.68|P-value:1.23E-11||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YME1(YPR024W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YMR086C-A(YMR086C-A_d)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL146W(YNL146W_p)|FD-Score:6.05|P-value:7.39E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR277C(YOR277C_d)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:AIM26(YKL037W)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:APJ1(YNL077W)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARG82(YDR173C)|FD-Score:6.05|P-value:7.26E-10||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARL3(YPL051W)|FD-Score:-4.31|P-value:8.21E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATS1(YAL020C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BDH2(YAL061W_p)|FD-Score:10.4|P-value:8.37E-26||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:BUD30(YDL151C_d)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CDC73(YLR418C)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG5(YNL051W)|FD-Score:-3.86|P-value:5.63E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ8(YGL119W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:COX15(YER141W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:CTF19(YPL018W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CTF8(YHR191C)|FD-Score:-4.28|P-value:9.17E-6||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:CTR1(YPR124W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CTR9(YOL145C)|FD-Score:5.34|P-value:4.71E-8||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CYC1(YJR048W)|FD-Score:5.51|P-value:1.82E-8||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:DOC1(YGL240W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:FET4(YMR319C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE4(YNR060W)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FUN14(YAL008W)|FD-Score:5.88|P-value:2.06E-9||SGD DESC:Mitochondrial protein of unknown function Gene:FUR4(YBR021W)|FD-Score:27.9|P-value:3.00E-171||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GLG2(YJL137C)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GND1(YHR183W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GTR2(YGR163W)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUF1(YLR289W)|FD-Score:4.28|P-value:9.42E-6||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HOS2(YGL194C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HPC2(YBR215W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:ICY2(YPL250C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:INO1(YJL153C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IRC21(YMR073C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LDB16(YCL005W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LRO1(YNR008W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LSM7(YNL147W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDM36(YPR083W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MDY2(YOL111C)|FD-Score:-4.88|P-value:5.19E-7||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MRP8(YKL142W)|FD-Score:-4.78|P-value:8.94E-7||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MRPS8(YMR158W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSN2(YMR037C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MTC5(YDR128W)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NMA111(YNL123W)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NOP16(YER002W)|FD-Score:5.26|P-value:7.33E-8||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:ORT1(YOR130C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:OSW2(YLR054C)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PAD1(YDR538W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PBP1(YGR178C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PEX27(YOR193W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PGC1(YPL206C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PIR3(YKL163W)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PMT2(YAL023C)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PRM5(YIL117C)|FD-Score:-3.92|P-value:4.40E-5||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PTK2(YJR059W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTP2(YOR208W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RAM1(YDL090C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RBG1(YAL036C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:REH1(YLR387C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIF1(YBR275C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPN4(YDL020C)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS19A(YOL121C)|FD-Score:-3.12|P-value:9.00E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RTT103(YDR289C)|FD-Score:3.85|P-value:5.88E-5||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAT4(YCR008W)|FD-Score:4.35|P-value:6.87E-6||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SBA1(YKL117W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress Gene:SCD6(YPR129W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SIT4(YDL047W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKN7(YHR206W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNF7(YLR025W)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPF1(YEL031W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSH1(YBR283C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SSU1(YPL092W)|FD-Score:-6.76|P-value:6.72E-12||SGD DESC:Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein Gene:STF1(YDL130W-A)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SVS1(YPL163C)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:4.9|P-value:4.77E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TOS8(YGL096W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication Gene:TRM10(YOL093W)|FD-Score:-5.59|P-value:1.14E-8||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:UBP13(YBL067C)|FD-Score:5.01|P-value:2.78E-7||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UTH1(YKR042W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VBA4(YDR119W_p)|FD-Score:3.8|P-value:7.09E-5||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VPS4(YPR173C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS61(YDR136C_d)|FD-Score:-4.66|P-value:1.58E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAP3(YHL009C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR174C(YBR174C_d)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR238C(YBR238C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR259W(YBR259W_p)|FD-Score:4.85|P-value:6.27E-7||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCL002C(YCL002C_p)|FD-Score:4.31|P-value:8.11E-6||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR087C-A(YCR087C-A_p)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL027C(YDL027C_p)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDL183C(YDL183C)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR278C(YDR278C_d)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YEL045C(YEL045C_d)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER119C-A(YER119C-A_d)|FD-Score:7.44|P-value:5.17E-14||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER156C(YER156C_p)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YFL012W(YFL012W_p)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGR111W(YGR111W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YIR021W-A(YIR021W-A_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YLR326W(YLR326W_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YMD8(YML038C)|FD-Score:-6.68|P-value:1.23E-11||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YME1(YPR024W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YMR086C-A(YMR086C-A_d)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL146W(YNL146W_p)|FD-Score:6.05|P-value:7.39E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR277C(YOR277C_d)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL150W12.605.68E-376.15RPC53RNA polymerase III subunit C53
YDL152W_d6.504.11E-110.84YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YOR224C5.667.62E-90.32RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YBR002C5.344.65E-80.25RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YKL112W5.091.82E-70.43ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YDR187C_d4.651.62E-60.05YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YPL143W4.612.05E-60.14RPL33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
YDR280W4.473.94E-60.48RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YHR058C3.993.29E-50.05MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
YLL008W3.944.02E-50.04DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
YOL094C3.904.79E-50.03RFC4Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YGL137W3.875.44E-50.02SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YBR202W3.855.83E-50.06MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YDR212W3.797.53E-50.06TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YFR029W3.739.41E-50.14PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR021W27.903.00E-171FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YAL061W_p10.408.37E-26BDH2_pPutative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
YER119C-A_d7.445.17E-14YER119C-A_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
YDR173C6.057.26E-10ARG82Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
YNL146W_p6.057.39E-10YNL146W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene
YAL008W5.882.06E-9FUN14Mitochondrial protein of unknown function
YLR418C5.601.05E-8CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YJR048W5.511.82E-8CYC1Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication
YOL145C5.344.71E-8CTR9Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats
YER002W5.267.33E-8NOP16Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YAL023C5.141.35E-7PMT2Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication
YBL067C5.012.78E-7UBP13Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
YBR069C4.904.77E-7TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YBR259W_p4.856.27E-7YBR259W_pProtein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
YOR277C_d4.711.23E-6YOR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20

GO enrichment analysis for SGTC_2393
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3241.38E-144SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.52381RNA processing & uracil transport
0.3132.25E-134SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.169492RNA processing & uracil transport
0.2781.46E-105SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.0704225RNA processing & uracil transport
0.2462.35E-82SGTC_22977971671 152.3 μMChembridge (Fragment library)9090160.25RNA processing & uracil transport
0.2421.57E-79SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.101695RNA processing & uracil transport
0.1991.65E-53SGTC_1817st052116 64.4 μMTimTec (Natural product derivative library)43613980.285714RNA processing & uracil transport
0.1871.56E-47SGTC_452diindolylmethane 8.0 μMICCB bioactive library30710.0816327RNA processing & uracil transport
0.1858.56E-47SGTC_486niguldipine 82.0 μMMiscellaneous12360.0888889amide catabolism
0.1845.37E-46SGTC_9231900-2434 73.3 μMChemDiv (Drug-like library)59105700.04RNA processing & uracil transport
0.1817.99E-45SGTC_33559151058 44.0 μMChembridge (Drug-like library)170157280.102941RNA processing & uracil transport
0.1776.87E-43SGTC_8093998-0021 90.0 μMChemDiv (Drug-like library)7154850.0923077RNA processing & uracil transport
0.1681.40E-38SGTC_530973-0023 40.0 μMChemDiv (Drug-like library)6747680.114754RNA processing & uracil transport
0.1667.59E-38SGTC_1167k781-0610 148.0 μMChemDiv (Drug-like library)45251690.0757576RNA processing & uracil transport
0.1603.44E-35SGTC_1950st077106 7.2 μMTimTec (Natural product derivative library)138318640.0722892RNA processing & uracil transport
0.1561.81E-33SGTC_1774st048371 48.5 μMTimTec (Natural product derivative library)42427270.078125RNA processing & uracil transport

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22206782180200 μM0.534884833803Chembridge (Fragment library)207.18606-0.26124RNA processing & uracil transport
SGTC_2351902685872.31 μM0.523811409300Chembridge (Fragment library)231.250520.87923RNA processing & uracil transport
SGTC_21045389443200 μM0.3404262244536Chembridge (Fragment library)236.1991630.94714
SGTC_23427990637118.86 μM0.3333332033102Chembridge (Fragment library)242.23341.07123RNA processing & uracil transport
SGTC_1817st05211664.4 μM0.2857144361398TimTec (Natural product derivative library)310.73291.85514RNA processing & uracil transport
SGTC_13161272-005065.9 μM0.2549027340392ChemDiv (Drug-like library)251.283222.02213
SGTC_22927671323168.05 μM0.254545937504Chembridge (Fragment library)251.2800032.02324RNA processing & uracil transport
SGTC_1776st04924945.9 μM0.2539681822314TimTec (Natural product derivative library)435.473944.73613
SGTC_22977971671152.32 μM0.25909016Chembridge (Fragment library)256.259981.39323RNA processing & uracil transport
SGTC_544k015-002052.4 μM0.2352943008304ChemDiv (Drug-like library)316.14943.20802