st077333

2-amino-6-(hydroxymethyl)-4-naphthalen-1-yl-8-oxo-4H-pyrano[3,2-b]pyran-3-carbonitrile

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2415
Screen concentration 57.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 2766255
SMILES C1=CC=C2C(=C1)C=CC=C2C3C(=C(OC4=C3OC(=CC4=O)CO)N)C#N
Standardized SMILES NC1=C(C#N)C(C2=C(O1)C(=O)C=C(CO)O2)c3cccc4ccccc34
Molecular weight 346.3362
ALogP 1.23
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.03
% growth inhibition (Hom. pool) -1.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2766255
Download HIP data (tab-delimited text)  (excel)
Gene:APC4(YDR118W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.05||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:ATM1(YMR301C)|FD-Score:-3.79|P-value:7.44E-5|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CCL1(YPR025C)|FD-Score:4.33|P-value:7.38E-6|Clearance:0.14||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CDC11(YJR076C)|FD-Score:5.98|P-value:1.09E-9|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC13(YDL220C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CDC53(YDL132W)|FD-Score:-4.55|P-value:2.69E-6|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CIA1(YDR267C)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:DAD2(YKR083C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.09||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ENP1(YBR247C)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:ERG1(YGR175C)|FD-Score:-4.44|P-value:4.51E-6|Clearance:0||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG25(YGR060W)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:FOL1(YNL256W)|FD-Score:5.52|P-value:1.71E-8|Clearance:0.23||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:FRS2(YFL022C)|FD-Score:-5.9|P-value:1.86E-9|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GCD1(YOR260W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.06||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:3.93|P-value:4.22E-5|Clearance:0.16||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM3(YDL205C)|FD-Score:5.29|P-value:6.28E-8|Clearance:0.07||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:LIP1(YMR298W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.17||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MED11(YMR112C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0.29||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:MES1(YGR264C)|FD-Score:3.42|P-value:3.17E-4|Clearance:0.03||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NDC1(YML031W)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NOP58(YOR310C)|FD-Score:5.14|P-value:1.37E-7|Clearance:0.47||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUS1(YDL193W)|FD-Score:-3.84|P-value:6.12E-5|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PCM1(YEL058W)|FD-Score:5.61|P-value:1.00E-8|Clearance:0.09||SGD DESC:Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors Gene:PHS1(YJL097W)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.08||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:POP7(YBR167C)|FD-Score:4.37|P-value:6.17E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE2(YPR103W)|FD-Score:5.21|P-value:9.44E-8|Clearance:0.07||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRP22(YER013W)|FD-Score:4.37|P-value:6.25E-6|Clearance:0.04||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RBA50(YDR527W)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.08||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RNR2(YJL026W)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.17||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPN8(YOR261C)|FD-Score:3.77|P-value:8.01E-5|Clearance:0.13||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RRP3(YHR065C)|FD-Score:5.97|P-value:1.17E-9|Clearance:0.36||SGD DESC:Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity Gene:SEC39(YLR440C)|FD-Score:4.67|P-value:1.52E-6|Clearance:0.01||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SNU56(YDR240C)|FD-Score:-3.79|P-value:7.42E-5|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SPN1(YPR133C)|FD-Score:-3.93|P-value:4.28E-5|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TIF5(YPR041W)|FD-Score:4|P-value:3.17E-5|Clearance:0.07||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:YCS4(YLR272C)|FD-Score:7.97|P-value:8.22E-16|Clearance:1.98||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YML6(YML025C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YOR203W(YOR203W_d)|FD-Score:4.02|P-value:2.88E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPD1(YDL235C)|FD-Score:-4.31|P-value:8.13E-6|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YPI1(YFR003C)|FD-Score:-3.36|P-value:3.88E-4|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:APC4(YDR118W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.05||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:ATM1(YMR301C)|FD-Score:-3.79|P-value:7.44E-5|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CCL1(YPR025C)|FD-Score:4.33|P-value:7.38E-6|Clearance:0.14||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CDC11(YJR076C)|FD-Score:5.98|P-value:1.09E-9|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC13(YDL220C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CDC53(YDL132W)|FD-Score:-4.55|P-value:2.69E-6|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CIA1(YDR267C)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:DAD2(YKR083C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.09||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ENP1(YBR247C)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:ERG1(YGR175C)|FD-Score:-4.44|P-value:4.51E-6|Clearance:0||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG25(YGR060W)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:FOL1(YNL256W)|FD-Score:5.52|P-value:1.71E-8|Clearance:0.23||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:FRS2(YFL022C)|FD-Score:-5.9|P-value:1.86E-9|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GCD1(YOR260W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.06||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:3.93|P-value:4.22E-5|Clearance:0.16||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM3(YDL205C)|FD-Score:5.29|P-value:6.28E-8|Clearance:0.07||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:LIP1(YMR298W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.17||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MED11(YMR112C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0.29||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:MES1(YGR264C)|FD-Score:3.42|P-value:3.17E-4|Clearance:0.03||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NDC1(YML031W)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NOP58(YOR310C)|FD-Score:5.14|P-value:1.37E-7|Clearance:0.47||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUS1(YDL193W)|FD-Score:-3.84|P-value:6.12E-5|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PCM1(YEL058W)|FD-Score:5.61|P-value:1.00E-8|Clearance:0.09||SGD DESC:Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors Gene:PHS1(YJL097W)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.08||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:POP7(YBR167C)|FD-Score:4.37|P-value:6.17E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE2(YPR103W)|FD-Score:5.21|P-value:9.44E-8|Clearance:0.07||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRP22(YER013W)|FD-Score:4.37|P-value:6.25E-6|Clearance:0.04||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RBA50(YDR527W)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.08||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RNR2(YJL026W)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.17||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPN8(YOR261C)|FD-Score:3.77|P-value:8.01E-5|Clearance:0.13||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RRP3(YHR065C)|FD-Score:5.97|P-value:1.17E-9|Clearance:0.36||SGD DESC:Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity Gene:SEC39(YLR440C)|FD-Score:4.67|P-value:1.52E-6|Clearance:0.01||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SNU56(YDR240C)|FD-Score:-3.79|P-value:7.42E-5|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SPN1(YPR133C)|FD-Score:-3.93|P-value:4.28E-5|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TIF5(YPR041W)|FD-Score:4|P-value:3.17E-5|Clearance:0.07||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:YCS4(YLR272C)|FD-Score:7.97|P-value:8.22E-16|Clearance:1.98||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YML6(YML025C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YOR203W(YOR203W_d)|FD-Score:4.02|P-value:2.88E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPD1(YDL235C)|FD-Score:-4.31|P-value:8.13E-6|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YPI1(YFR003C)|FD-Score:-3.36|P-value:3.88E-4|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2766255
Download HOP data (tab-delimited text)  (excel)
Gene:ACA1(YER045C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ACF4(YJR083C)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADP1(YCR011C)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AFT1(YGL071W)|FD-Score:9.35|P-value:4.49E-21||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AFT2(YPL202C)|FD-Score:4.91|P-value:4.56E-7||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AIM11(YER093C-A)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ALD4(YOR374W)|FD-Score:4.28|P-value:9.19E-6||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:ANP1(YEL036C)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ARE2(YNR019W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARG2(YJL071W)|FD-Score:3.47|P-value:2.55E-4||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ASN1(YPR145W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:ATG22(YCL038C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:BNI1(YNL271C)|FD-Score:7.18|P-value:3.40E-13||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BOI1(YBL085W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:7.49|P-value:3.55E-14||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CBP6(YBR120C)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CHZ1(YER030W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CLD1(YGR110W)|FD-Score:-3.87|P-value:5.44E-5||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CNB1(YKL190W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CNL1(YDR357C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:COQ5(YML110C)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:COX11(YPL132W)|FD-Score:4.79|P-value:8.38E-7||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:CRH1(YGR189C)|FD-Score:8.69|P-value:1.81E-18||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CTS1(YLR286C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:CUS2(YNL286W)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:CYM1(YDR430C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Gene:DBP7(YKR024C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DID4(YKL002W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DLD1(YDL174C)|FD-Score:4.97|P-value:3.37E-7||SGD DESC:D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane Gene:DNF1(YER166W)|FD-Score:-3.82|P-value:6.65E-5||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EAF1(YDR359C)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EAF6(YJR082C)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECL1(YGR146C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ECM23(YPL021W)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EMC2(YJR088C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:ENO1(YGR254W)|FD-Score:3.72|P-value:9.77E-5||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EST1(YLR233C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:ETT1(YOR051C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FAR11(YNL127W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FIN1(YDR130C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FMP46(YKR049C)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP48(YGR052W_p)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FSH3(YOR280C)|FD-Score:6.51|P-value:3.69E-11||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FTH1(YBR207W)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress Gene:GAT3(YLR013W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDT1(YBR187W_p)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GSM1(YJL103C)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:HDA2(YDR295C)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:HDA3(YPR179C)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HHT1(YBR010W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HMS2(YJR147W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HSL7(YBR133C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:LHS1(YKL073W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LOT6(YLR011W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions Gene:MBA1(YBR185C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MCR1(YKL150W)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MDH1(YKL085W)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MDM12(YOL009C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MRPS35(YGR165W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NGL2(YMR285C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NIT1(YIL164C)|FD-Score:5.54|P-value:1.54E-8||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NKP1(YDR383C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NNK1(YKL171W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm Gene:OXP1(YKL215C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OYE3(YPL171C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PBS2(YJL128C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PHB2(YGR231C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PHO84(YML123C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PKH3(YDR466W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:POR1(YNL055C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RPL21A(YBR191W)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPS14B(YJL191W)|FD-Score:-3.75|P-value:8.69E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:-3.72|P-value:9.93E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS26B(YER131W)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RUB1(YDR139C)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SAP190(YKR028W)|FD-Score:-4.3|P-value:8.58E-6||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SHH3(YMR118C_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SKI7(YOR076C)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SKM1(YOL113W)|FD-Score:6.4|P-value:7.99E-11||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLX5(YDL013W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SMF3(YLR034C)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SNF11(YDR073W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SOD1(YJR104C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO7(YAL009W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:STE20(YHL007C)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:STP2(YHR006W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:TDA4(YJR116W_p)|FD-Score:6.42|P-value:6.81E-11||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele Gene:TOF1(YNL273W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TRR2(YHR106W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:TSL1(YML100W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:TUL1(YKL034W)|FD-Score:6.12|P-value:4.75E-10||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBP2(YOR124C)|FD-Score:5.28|P-value:6.53E-8||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VMA4(YOR332W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS13(YLL040C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS45(YGL095C)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XYL2(YLR070C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBR012C(YBR012C_d)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR219C(YBR219C_p)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR476C(YDR476C_p)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YFL051C(YFL051C_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YFR039C(YFR039C_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL108C(YGL108C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGL176C(YGL176C_p)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGL185C(YGL185C_p)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR250C(YGR250C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJL169W(YJL169W_d)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKL044W(YKL044W_p)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL050C(YKL050C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p Gene:YKL169C(YKL169C_d)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKL222C(YKL222C)|FD-Score:6.26|P-value:1.97E-10||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YKR033C(YKR033C_d)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YKU70(YMR284W)|FD-Score:4.87|P-value:5.57E-7||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YME1(YPR024W)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YML122C(YML122C_d)|FD-Score:9.74|P-value:1.06E-22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR007W(YMR007W_d)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL146W(YNL146W_p)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YNL324W(YNL324W_d)|FD-Score:-4.66|P-value:1.55E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL046C(YOL046C_d)|FD-Score:7.34|P-value:1.04E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR053W(YOR053W_d)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR062C(YOR062C_p)|FD-Score:7.66|P-value:9.10E-15||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YOR072W(YOR072W_d)|FD-Score:4.78|P-value:8.72E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL025C(YPL025C_d)|FD-Score:-5.62|P-value:9.69E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL073C(YPL073C_d)|FD-Score:5.41|P-value:3.07E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPR123C(YPR123C_d)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR153W(YPR153W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative protein of unknown function Gene:YRB30(YGL164C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:YTA6(YPL074W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ZDS1(YMR273C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication Gene:ACA1(YER045C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ACF4(YJR083C)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADP1(YCR011C)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AFT1(YGL071W)|FD-Score:9.35|P-value:4.49E-21||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AFT2(YPL202C)|FD-Score:4.91|P-value:4.56E-7||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AIM11(YER093C-A)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ALD4(YOR374W)|FD-Score:4.28|P-value:9.19E-6||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:ANP1(YEL036C)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ARE2(YNR019W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARG2(YJL071W)|FD-Score:3.47|P-value:2.55E-4||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ASN1(YPR145W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:ATG22(YCL038C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:BNI1(YNL271C)|FD-Score:7.18|P-value:3.40E-13||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BOI1(YBL085W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:7.49|P-value:3.55E-14||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CBP6(YBR120C)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CHZ1(YER030W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CLD1(YGR110W)|FD-Score:-3.87|P-value:5.44E-5||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CNB1(YKL190W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CNL1(YDR357C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:COQ5(YML110C)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:COX11(YPL132W)|FD-Score:4.79|P-value:8.38E-7||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:CRH1(YGR189C)|FD-Score:8.69|P-value:1.81E-18||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CTS1(YLR286C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:CUS2(YNL286W)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:CYM1(YDR430C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Gene:DBP7(YKR024C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DID4(YKL002W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DLD1(YDL174C)|FD-Score:4.97|P-value:3.37E-7||SGD DESC:D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane Gene:DNF1(YER166W)|FD-Score:-3.82|P-value:6.65E-5||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EAF1(YDR359C)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EAF6(YJR082C)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECL1(YGR146C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ECM23(YPL021W)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EMC2(YJR088C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:ENO1(YGR254W)|FD-Score:3.72|P-value:9.77E-5||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EST1(YLR233C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:ETT1(YOR051C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FAR11(YNL127W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FIN1(YDR130C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FMP46(YKR049C)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP48(YGR052W_p)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FSH3(YOR280C)|FD-Score:6.51|P-value:3.69E-11||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FTH1(YBR207W)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress Gene:GAT3(YLR013W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDT1(YBR187W_p)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GSM1(YJL103C)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:HDA2(YDR295C)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:HDA3(YPR179C)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HHT1(YBR010W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HMS2(YJR147W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HSL7(YBR133C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:LHS1(YKL073W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LOT6(YLR011W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions Gene:MBA1(YBR185C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MCR1(YKL150W)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MDH1(YKL085W)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MDM12(YOL009C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MRPS35(YGR165W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NGL2(YMR285C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NIT1(YIL164C)|FD-Score:5.54|P-value:1.54E-8||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NKP1(YDR383C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NNK1(YKL171W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm Gene:OXP1(YKL215C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OYE3(YPL171C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PBS2(YJL128C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PHB2(YGR231C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PHO84(YML123C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PKH3(YDR466W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:POR1(YNL055C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RPL21A(YBR191W)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPS14B(YJL191W)|FD-Score:-3.75|P-value:8.69E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:-3.72|P-value:9.93E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS26B(YER131W)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RUB1(YDR139C)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SAP190(YKR028W)|FD-Score:-4.3|P-value:8.58E-6||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SHH3(YMR118C_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SKI7(YOR076C)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SKM1(YOL113W)|FD-Score:6.4|P-value:7.99E-11||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLX5(YDL013W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SMF3(YLR034C)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SNF11(YDR073W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SOD1(YJR104C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO7(YAL009W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:STE20(YHL007C)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:STP2(YHR006W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:TDA4(YJR116W_p)|FD-Score:6.42|P-value:6.81E-11||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele Gene:TOF1(YNL273W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TRR2(YHR106W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:TSL1(YML100W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:TUL1(YKL034W)|FD-Score:6.12|P-value:4.75E-10||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBP2(YOR124C)|FD-Score:5.28|P-value:6.53E-8||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VMA4(YOR332W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS13(YLL040C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS45(YGL095C)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XYL2(YLR070C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBR012C(YBR012C_d)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR219C(YBR219C_p)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR476C(YDR476C_p)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YFL051C(YFL051C_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YFR039C(YFR039C_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL108C(YGL108C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGL176C(YGL176C_p)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGL185C(YGL185C_p)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR250C(YGR250C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJL169W(YJL169W_d)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKL044W(YKL044W_p)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL050C(YKL050C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p Gene:YKL169C(YKL169C_d)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKL222C(YKL222C)|FD-Score:6.26|P-value:1.97E-10||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YKR033C(YKR033C_d)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YKU70(YMR284W)|FD-Score:4.87|P-value:5.57E-7||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YME1(YPR024W)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YML122C(YML122C_d)|FD-Score:9.74|P-value:1.06E-22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR007W(YMR007W_d)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL146W(YNL146W_p)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YNL324W(YNL324W_d)|FD-Score:-4.66|P-value:1.55E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL046C(YOL046C_d)|FD-Score:7.34|P-value:1.04E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR053W(YOR053W_d)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR062C(YOR062C_p)|FD-Score:7.66|P-value:9.10E-15||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YOR072W(YOR072W_d)|FD-Score:4.78|P-value:8.72E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL025C(YPL025C_d)|FD-Score:-5.62|P-value:9.69E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL073C(YPL073C_d)|FD-Score:5.41|P-value:3.07E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPR123C(YPR123C_d)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR153W(YPR153W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative protein of unknown function Gene:YRB30(YGL164C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:YTA6(YPL074W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ZDS1(YMR273C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR272C7.978.22E-161.98YCS4Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YJR076C5.981.09E-90.01CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YHR065C5.971.17E-90.36RRP3Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
YEL058W5.611.00E-80.09PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YNL256W5.521.71E-80.23FOL1Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
YDL205C5.296.28E-80.07HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YPR103W5.219.44E-80.07PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YOR310C5.141.37E-70.47NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YLR440C4.671.52E-60.01SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YMR112C4.661.58E-60.29MED11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
YBR167C4.376.17E-60.00POP7Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YER013W4.376.25E-60.04PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YPR025C4.337.38E-60.14CCL1Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
YJL026W4.201.35E-50.17RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YOR203W_d4.022.88E-50.02YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML122C_d9.741.06E-22YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL071W9.354.49E-21AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YGR189C8.691.81E-18CRH1Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
YOR062C_p7.669.10E-15YOR062C_pProtein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YPL069C7.493.55E-14BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YOL046C_d7.341.04E-13YOL046C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W
YNL271C7.183.40E-13BNI1Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
YOR280C6.513.69E-11FSH3Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2
YJR116W_p6.426.81E-11TDA4_pPutative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
YOL113W6.407.99E-11SKM1Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth
YKL222C6.261.97E-10YKL222CProtein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
YKL034W6.124.75E-10TUL1Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1
YIL164C5.541.54E-8NIT1Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene
YDR359C5.511.77E-8EAF1Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1
YPL073C_d5.413.07E-8YPL073C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments

GO enrichment analysis for SGTC_2415
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0977.66E-14SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.0689655RNA pol III & RNase P/MRP
0.0765.92E-9SGTC_31199125183 49.5 μMChembridge (Drug-like library)226953340.105263
0.0732.00E-8SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.0869565
0.0732.19E-8SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.108434amide catabolism
0.0706.57E-8SGTC_482fipronil 114.0 μMICCB bioactive library33520.107143
0.0708.73E-8SGTC_2724maprotiline 69.6 μMMiscellaneous40110.12ERG2
0.0681.41E-7SGTC_2533agelasine 13.7 μMMicrosource (Natural product library)166677450.0550459ERG2
0.0663.32E-7SGTC_2734clemastine 41.6 μMMiscellaneous269870.0823529ERG2
0.0663.33E-7SGTC_12630774-1208 64.8 μMChemDiv (Drug-like library)9065850.133333
0.0654.99E-7SGTC_24745321570 43.3 μMMiscellaneous28413840.103896
0.0655.74E-7SGTC_31779107506 49.5 μMChembridge (Drug-like library)88971940.0804598ERG2
0.0612.80E-6SGTC_9591222-0044 107.0 μMChemDiv (Drug-like library)60836120.0789474
0.0613.07E-6SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0410959ERG2
0.0604.60E-6SGTC_2640pentamidine 10.4 μMMiscellaneous47350.0714286mitochondrial processes
0.0595.16E-6SGTC_1924st058459 75.1 μMTimTec (Natural product derivative library)6886830.146667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1668st01491451.6 μM0.5396832839556TimTec (Natural product derivative library)366.368863.50415RPP1 & pyrimidine depletion
SGTC_1988st07187253.4 μM0.45205516414116TimTec (Natural product derivative library)374.301660.19729
SGTC_1981st07182647 μM0.4411838600TimTec (Natural product derivative library)425.355231.971210
SGTC_1979st07180945.3 μM0.4054054976333TimTec (Natural product derivative library)398.194221.63127RPP1 & pyrimidine depletion
SGTC_1989st07186850.2 μM0.40540516414057TimTec (Natural product derivative library)398.194221.63127
SGTC_1980st07181145.7 μM0.3947374974850TimTec (Natural product derivative library)412.22081.9827RPP1 & pyrimidine depletion
SGTC_1983st07181346.9 μM0.3896111838595TimTec (Natural product derivative library)426.247382.35827RPP1 & pyrimidine depletion
SGTC_1986st07181245 μM0.37662311838594TimTec (Natural product derivative library)426.247382.35827
SGTC_2067522462934.08 μM0.3333332835000Chembridge (Fragment library)288.728922.15414
SGTC_1662st01405452 μM0.3289472838777TimTec (Natural product derivative library)360.362742.92816TSC3-RPN4