aureobasidin a

3,6-dibenzyl-12,24-di(butan-2-yl)-15-(2-hydroxypropan-2-yl)-4,10,16,22-tetramethyl-18-(2-methylpropyl)-9,21-di(propan-2-yl)-13-oxa-1,4,7,10,16,19,22,25-octazabicyclo[25.3.0]triacontane-2,5,8,11,14,17,20,23,26-nonone


Established Yeast Drug Target : AUR1 KEI1

Aureobasidium a is a potent inhibitor of inositol phosphorylceramide (IPC) synthase in fungi.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2420
Screen concentration 50.0 nM
Source Miscellaneous
PubChem CID 9963430
SMILES CCC(C)C1C(=O)N(C(C(=O)NC(C(=O)N(C(C(=O)OC(C(=O)N(C(C(=O)NC(C(=O)N(C(C(=O)N2CCCC2C(=O)N1)CC3=CC=CC=C3)C)CC4=CC=CC=C4)C(C)C)C)C(C)CC)C(C)(C)O)C)CC(C)C)C(C)C)C
Standardized SMILES CCC(C)C1NC(=O)C2CCCN2C(=O)C(Cc3ccccc3)N(C)C(=O)C(Cc4ccccc4)NC(=O)C(C(C)C)N(C)C(=O)C(OC(=O)C(N(C)C(=O)C(CC(C)C)NC(=O)C(C(C)C)N(C)C1=O)C(C)(C)O)C(C)CC
Molecular weight 1101.4195
ALogP 6.01
H-bond donor count 4
H-bond acceptor count 11
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -3.99


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 9963430
Download HIP data (tab-delimited text)  (excel)
Gene:BRR6(YGL247W)|FD-Score:-4.77|P-value:9.32E-7|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CAK1(YFL029C)|FD-Score:-4.06|P-value:2.45E-5|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC11(YJR076C)|FD-Score:-3.98|P-value:3.48E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DML1(YMR211W)|FD-Score:10.6|P-value:1.88E-26|Clearance:6.25||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:11.1|P-value:1.07E-28|Clearance:0.47||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAF1(YIL019W)|FD-Score:-3.14|P-value:8.32E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:KEI1(YDR367W)|FD-Score:45.5|P-value:0|Clearance:34.43||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MTR2(YKL186C)|FD-Score:-3.14|P-value:8.35E-4|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NUP49(YGL172W)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:RFA3(YJL173C)|FD-Score:-4.72|P-value:1.17E-6|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RHO1(YPR165W)|FD-Score:3.2|P-value:6.82E-4|Clearance:0.11||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:SCC2(YDR180W)|FD-Score:4.33|P-value:7.46E-6|Clearance:1.01||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SPC42(YKL042W)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SWI1(YPL016W)|FD-Score:3.32|P-value:4.49E-4|Clearance:0.12||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:THG1(YGR024C)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:BRR6(YGL247W)|FD-Score:-4.77|P-value:9.32E-7|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CAK1(YFL029C)|FD-Score:-4.06|P-value:2.45E-5|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC11(YJR076C)|FD-Score:-3.98|P-value:3.48E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DML1(YMR211W)|FD-Score:10.6|P-value:1.88E-26|Clearance:6.25||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:11.1|P-value:1.07E-28|Clearance:0.47||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAF1(YIL019W)|FD-Score:-3.14|P-value:8.32E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:KEI1(YDR367W)|FD-Score:45.5|P-value:0|Clearance:34.43||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MTR2(YKL186C)|FD-Score:-3.14|P-value:8.35E-4|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NUP49(YGL172W)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:RFA3(YJL173C)|FD-Score:-4.72|P-value:1.17E-6|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RHO1(YPR165W)|FD-Score:3.2|P-value:6.82E-4|Clearance:0.11||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:SCC2(YDR180W)|FD-Score:4.33|P-value:7.46E-6|Clearance:1.01||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SPC42(YKL042W)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SWI1(YPL016W)|FD-Score:3.32|P-value:4.49E-4|Clearance:0.12||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:THG1(YGR024C)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 9963430
Download HOP data (tab-delimited text)  (excel)
Gene:ACF4(YJR083C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ANP1(YEL036C)|FD-Score:-4.27|P-value:9.71E-6||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APJ1(YNL077W)|FD-Score:4.88|P-value:5.18E-7||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARF1(YDL192W)|FD-Score:4.91|P-value:4.58E-7||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ATG20(YDL113C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:ATO2(YNR002C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BDS1(YOL164W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BRO1(YPL084W)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CLC1(YGR167W)|FD-Score:5.72|P-value:5.41E-9||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNB1(YKL190W)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CNM67(YNL225C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COX14(YML129C)|FD-Score:4.43|P-value:4.70E-6||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CST6(YIL036W)|FD-Score:5.71|P-value:5.55E-9||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:CYC7(YEL039C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAL4(YIR028W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DIA3(YDL024C)|FD-Score:4.3|P-value:8.59E-6||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DSD1(YGL196W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:EAP1(YKL204W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECL1(YGR146C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EPS1(YIL005W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FIS1(YIL065C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FMP30(YPL103C)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FMP48(YGR052W_p)|FD-Score:5.75|P-value:4.48E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FRD1(YEL047C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GAS1(YMR307W)|FD-Score:-4.77|P-value:9.00E-7||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GAT3(YLR013W)|FD-Score:4.55|P-value:2.62E-6||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GLN3(YER040W)|FD-Score:5.32|P-value:5.29E-8||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GUP2(YPL189W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:HIS6(YIL020C)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HUB1(YNR032C-A)|FD-Score:7.19|P-value:3.32E-13||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:ICS2(YBR157C)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IFA38(YBR159W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IVY1(YDR229W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:JEM1(YJL073W)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KNS1(YLL019C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:KTR1(YOR099W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:KTR7(YIL085C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:6.55|P-value:2.91E-11||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MDY2(YOL111C)|FD-Score:4.76|P-value:9.67E-7||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MMP1(YLL061W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MPM1(YJL066C)|FD-Score:-3.8|P-value:7.23E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRP1(YDR347W)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPL23(YOR150W)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH1(YHR120W)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MTF2(YDL044C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NOT3(YIL038C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OAC1(YKL120W)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OM45(YIL136W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:PET117(YER058W)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PET309(YLR067C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PFS1(YHR185C)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHO89(YBR296C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PKR1(YMR123W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMP1(YCR024C-A)|FD-Score:7.8|P-value:3.01E-15||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-4.44|P-value:4.51E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PUB1(YNL016W)|FD-Score:4.5|P-value:3.36E-6||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:QCR2(YPR191W)|FD-Score:-3.9|P-value:4.76E-5||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:RAD18(YCR066W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:REC102(YLR329W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:REX2(YLR059C)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RGA1(YOR127W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIF2(YLR453C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL26A(YLR344W)|FD-Score:7.25|P-value:2.10E-13||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPP2A(YOL039W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:SAK1(YER129W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SEG2(YKL105C_p)|FD-Score:9.15|P-value:2.97E-20||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SIP18(YMR175W)|FD-Score:-4.99|P-value:3.08E-7||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SNF7(YLR025W)|FD-Score:10.2|P-value:1.26E-24||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOP4(YJL192C)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRL1(YOR247W)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SUL1(YBR294W)|FD-Score:8.89|P-value:3.15E-19||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUR4(YLR372W)|FD-Score:11.6|P-value:2.77E-31||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.8|P-value:5.66E-23||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TSR2(YLR435W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBA4(YHR111W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UPS2(YLR168C)|FD-Score:-4.03|P-value:2.75E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VBA2(YBR293W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA7(YGR020C)|FD-Score:4.84|P-value:6.66E-7||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS25(YJR102C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:7.27|P-value:1.86E-13||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS63(YLR261C_d)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:WAR1(YML076C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YAT1(YAR035W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBR232C(YBR232C_d)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCK1(YHR135C)|FD-Score:4.89|P-value:5.10E-7||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCL001W-A(YCL001W-A_p)|FD-Score:-3.23|P-value:6.30E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR001W(YCR001W_d)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR101C(YCR101C_p)|FD-Score:-3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YCR102W-A(YCR102W-A_d)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR340W(YDR340W_d)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Putative protein of unknown function Gene:YFH7(YFR007W)|FD-Score:4.84|P-value:6.40E-7||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGL072C(YGL072C_d)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YHR022C-A(YHR022C-A_p)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YHR045W(YHR045W_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YJL119C(YJL119C_d)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKE4(YIL023C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKR011C(YKR011C_p)|FD-Score:-3.92|P-value:4.40E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLL053C(YLL053C_p)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR126C(YLR126C_p)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YMR317W(YMR317W_p)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YOL036W(YOL036W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOR325W(YOR325W_d)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YOR385W(YOR385W_p)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL229W(YPL229W_p)|FD-Score:6.73|P-value:8.24E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR098C(YPR098C)|FD-Score:5.41|P-value:3.15E-8||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YRF1-6(YNL339C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:YSP1(YHR155W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YSP3(YOR003W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ACF4(YJR083C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ANP1(YEL036C)|FD-Score:-4.27|P-value:9.71E-6||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APJ1(YNL077W)|FD-Score:4.88|P-value:5.18E-7||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARF1(YDL192W)|FD-Score:4.91|P-value:4.58E-7||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ATG20(YDL113C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:ATO2(YNR002C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BDS1(YOL164W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BRO1(YPL084W)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CLC1(YGR167W)|FD-Score:5.72|P-value:5.41E-9||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNB1(YKL190W)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CNM67(YNL225C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COX14(YML129C)|FD-Score:4.43|P-value:4.70E-6||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CST6(YIL036W)|FD-Score:5.71|P-value:5.55E-9||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:CYC7(YEL039C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAL4(YIR028W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DIA3(YDL024C)|FD-Score:4.3|P-value:8.59E-6||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DSD1(YGL196W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:EAP1(YKL204W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECL1(YGR146C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EPS1(YIL005W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FIS1(YIL065C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FMP30(YPL103C)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FMP48(YGR052W_p)|FD-Score:5.75|P-value:4.48E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FRD1(YEL047C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GAS1(YMR307W)|FD-Score:-4.77|P-value:9.00E-7||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GAT3(YLR013W)|FD-Score:4.55|P-value:2.62E-6||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GLN3(YER040W)|FD-Score:5.32|P-value:5.29E-8||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GUP2(YPL189W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:HIS6(YIL020C)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HUB1(YNR032C-A)|FD-Score:7.19|P-value:3.32E-13||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:ICS2(YBR157C)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IFA38(YBR159W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IVY1(YDR229W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:JEM1(YJL073W)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KNS1(YLL019C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:KTR1(YOR099W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:KTR7(YIL085C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:6.55|P-value:2.91E-11||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MDY2(YOL111C)|FD-Score:4.76|P-value:9.67E-7||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MMP1(YLL061W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MPM1(YJL066C)|FD-Score:-3.8|P-value:7.23E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRP1(YDR347W)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPL23(YOR150W)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH1(YHR120W)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MTF2(YDL044C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NOT3(YIL038C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OAC1(YKL120W)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OM45(YIL136W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:PET117(YER058W)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PET309(YLR067C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PFS1(YHR185C)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHO89(YBR296C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PKR1(YMR123W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMP1(YCR024C-A)|FD-Score:7.8|P-value:3.01E-15||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-4.44|P-value:4.51E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PUB1(YNL016W)|FD-Score:4.5|P-value:3.36E-6||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:QCR2(YPR191W)|FD-Score:-3.9|P-value:4.76E-5||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:RAD18(YCR066W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:REC102(YLR329W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:REX2(YLR059C)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RGA1(YOR127W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIF2(YLR453C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL26A(YLR344W)|FD-Score:7.25|P-value:2.10E-13||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPP2A(YOL039W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:SAK1(YER129W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SEG2(YKL105C_p)|FD-Score:9.15|P-value:2.97E-20||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SIP18(YMR175W)|FD-Score:-4.99|P-value:3.08E-7||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SNF7(YLR025W)|FD-Score:10.2|P-value:1.26E-24||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOP4(YJL192C)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRL1(YOR247W)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SUL1(YBR294W)|FD-Score:8.89|P-value:3.15E-19||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUR4(YLR372W)|FD-Score:11.6|P-value:2.77E-31||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.8|P-value:5.66E-23||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TSR2(YLR435W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBA4(YHR111W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UPS2(YLR168C)|FD-Score:-4.03|P-value:2.75E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VBA2(YBR293W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA7(YGR020C)|FD-Score:4.84|P-value:6.66E-7||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS25(YJR102C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:7.27|P-value:1.86E-13||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS63(YLR261C_d)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:WAR1(YML076C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YAT1(YAR035W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBR232C(YBR232C_d)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCK1(YHR135C)|FD-Score:4.89|P-value:5.10E-7||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCL001W-A(YCL001W-A_p)|FD-Score:-3.23|P-value:6.30E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR001W(YCR001W_d)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR101C(YCR101C_p)|FD-Score:-3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YCR102W-A(YCR102W-A_d)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR340W(YDR340W_d)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Putative protein of unknown function Gene:YFH7(YFR007W)|FD-Score:4.84|P-value:6.40E-7||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGL072C(YGL072C_d)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YHR022C-A(YHR022C-A_p)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YHR045W(YHR045W_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YJL119C(YJL119C_d)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKE4(YIL023C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKR011C(YKR011C_p)|FD-Score:-3.92|P-value:4.40E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLL053C(YLL053C_p)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR126C(YLR126C_p)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YMR317W(YMR317W_p)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YOL036W(YOL036W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOR325W(YOR325W_d)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YOR385W(YOR385W_p)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL229W(YPL229W_p)|FD-Score:6.73|P-value:8.24E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR098C(YPR098C)|FD-Score:5.41|P-value:3.15E-8||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YRF1-6(YNL339C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:YSP1(YHR155W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YSP3(YOR003W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative precursor to the subtilisin-like protease III

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR367W45.50034.40KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YHR007C11.101.07E-280.47ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YMR211W10.601.88E-266.25DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YDR180W4.337.46E-61.01SCC2Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
YPL016W3.324.49E-40.12SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YPR165W3.206.82E-40.11RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YGL172W3.099.93E-40.01NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YGR065C3.080.001040.00VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
YER125W3.080.001050.03RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
YOL133W3.050.001150.08HRT1RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF
YOR257W2.960.001520.04CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YPR019W2.920.001720.10MCM4Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
YMR168C2.830.002350.00CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YBR142W2.820.002370.06MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YAR019C2.760.002890.06CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR372W11.602.77E-31SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YLR025W10.201.26E-24SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YBR058C-A9.805.66E-23TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YKL105C_p9.152.97E-20SEG2_pEisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes
YBR294W8.893.15E-19SUL1High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
YCR024C-A7.803.01E-15PMP1Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
YGL095C7.271.86E-13VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YLR344W7.252.10E-13RPL26ARibosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
YNR032C-A7.193.32E-13HUB1Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
YPL229W_p6.738.24E-12YPL229W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
YNL323W6.552.91E-11LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
YGR052W_p5.754.48E-9FMP48_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
YGR167W5.725.41E-9CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YIL036W5.715.55E-9CST6Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication
YIL020C5.531.56E-8HIS6Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts

GO enrichment analysis for SGTC_2420
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3875.12E-210SGTC_2567aureobasidin a 60.0 nMMiscellaneous99634301
0.1431.97E-28SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.148148
0.1411.57E-27SGTC_1106tegaserod maleate 11.7 μMNIH Clinical Collection69183690.046875
0.1395.74E-27SGTC_21645685496 200.0 μMChembridge (Fragment library)7590460.0900901
0.1309.09E-24SGTC_13441488-0097 29.1 μMChemDiv (Drug-like library)7479660.0530973
0.1286.13E-23SGTC_2740felodipine 17.1 μMMiscellaneous33330.0854701
0.1271.60E-22SGTC_24725377652 49.4 μMMiscellaneous16109030.0917431TRP & mitochondrial translation
0.1254.78E-22SGTC_1832st054113 55.0 μMTimTec (Natural product derivative library)12512670.0991736
0.1226.90E-21SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.1TSC3-RPN4
0.1194.74E-20SGTC_13651672-3266 55.4 μMChemDiv (Drug-like library)64158730.0859375
0.1163.58E-19SGTC_1568digitonin 820.0 nMTimTec (Pure natural product library)27350100.0429448sphingolipid biosynthesis & PDR1
0.1163.85E-19SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0775862TSC3-RPN4
0.1156.17E-19SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.0555556
0.1141.15E-18SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0714286TSC3-RPN4
0.1141.29E-18SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.097561TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2567aureobasidin a60 nM19963430Miscellaneous1101.419486.007411
SGTC_2661dactinomycin12.96 μM0.24637745006140Microsource (Natural product library)1255.417040.364518redox potentiating
SGTC_2658polymyxin b sulfate77.05 μM0.2428575702105Microsource (Natural product library)1301.5552-6.8732022NEO1
SGTC_1533dihydroergotamine mesylate29.4 μM0.21582771171TimTec (Pure natural product library)679.783042.78649DNA intercalators
SGTC_2721ifenprodil61.44 μM0.1941753689Miscellaneous325.444584.18523plasma membrane duress
SGTC_2007401153971.82 μM0.18367345595301Chembridge (Fragment library)285.180181.42712cell wall signaling
SGTC_10472043-304482 μM0.166667669592ChemDiv (Drug-like library)323.385643.66903
SGTC_2734clemastine41.6 μM0.16666726987Miscellaneous343.890244.80602ERG2
SGTC_2955907625412.21 μM0.16363616459619Chembridge (Drug-like library)316.394762.72414
SGTC_2649anisomycin9.56 μM0.1634626610309ICCB bioactive library235.278980.89324