st077119

4,8-dimethoxyfuro[2,3-b]quinoline

An active alkaloid of Chinese medicines from Dictamni radicis cortex (Rutaceae).

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2423
Screen concentration 87.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 107936
SMILES COC1=CC=CC2=C1N=C3C(=C2OC)C=CO3
Standardized SMILES COc1cccc2c(OC)c3ccoc3nc12
Molecular weight 229.2313
ALogP 2.49
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.04
% growth inhibition (Hom. pool) 1.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 107936
Download HIP data (tab-delimited text)  (excel)
Gene:MET4(YNL103W)|FD-Score:-4.11|P-value:2.01E-5|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MTR2(YKL186C)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.31||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NOP15(YNL110C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.13||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP2(YNL061W)|FD-Score:3.93|P-value:4.22E-5|Clearance:0.26||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RFC2(YJR068W)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:SSS1(YDR086C)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.1||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TCP1(YDR212W)|FD-Score:3.26|P-value:5.55E-4|Clearance:0.05||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:YJL009W(YJL009W_d)|FD-Score:4.09|P-value:2.11E-5|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YOR102W(YOR102W_d)|FD-Score:4.64|P-value:1.73E-6|Clearance:0.55||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:MET4(YNL103W)|FD-Score:-4.11|P-value:2.01E-5|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MTR2(YKL186C)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.31||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NOP15(YNL110C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.13||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP2(YNL061W)|FD-Score:3.93|P-value:4.22E-5|Clearance:0.26||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RFC2(YJR068W)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:SSS1(YDR086C)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.1||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TCP1(YDR212W)|FD-Score:3.26|P-value:5.55E-4|Clearance:0.05||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:YJL009W(YJL009W_d)|FD-Score:4.09|P-value:2.11E-5|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YOR102W(YOR102W_d)|FD-Score:4.64|P-value:1.73E-6|Clearance:0.55||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 107936
Download HOP data (tab-delimited text)  (excel)
Gene:ADK2(YER170W)|FD-Score:5.33|P-value:4.83E-8||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ANT1(YPR128C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ATP10(YLR393W)|FD-Score:-4.29|P-value:9.03E-6||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:AUA1(YFL010W-A)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BAP2(YBR068C)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BUD17(YNR027W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:BUD21(YOR078W)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:BUD26(YDR241W_d)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCE1(YKL011C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CDC10(YCR002C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CKI1(YLR133W)|FD-Score:4.58|P-value:2.34E-6||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:COQ2(YNR041C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CRC1(YOR100C)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation Gene:DCI1(YOR180C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DGR2(YKL121W_p)|FD-Score:-7.91|P-value:1.27E-15||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:ELP2(YGR200C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:FCY21(YER060W)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FLO1(YAR050W)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FYV7(YLR068W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:HIS5(YIL116W)|FD-Score:-5.86|P-value:2.34E-9||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IGO2(YHR132W-A)|FD-Score:-3.87|P-value:5.43E-5||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:INP53(YOR109W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:ITR1(YDR497C)|FD-Score:-3.1|P-value:9.79E-4||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:JLP2(YMR132C_p)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KCC4(YCL024W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:LAP3(YNL239W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LDB17(YDL146W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:MAL33(YBR297W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MDV1(YJL112W)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MEC3(YLR288C)|FD-Score:-6.76|P-value:6.78E-12||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MRPL23(YOR150W)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NHA1(YLR138W)|FD-Score:-4.81|P-value:7.40E-7||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:NUP84(YDL116W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PNP1(YLR209C)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PPZ2(YDR436W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PTR2(YKR093W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAI1(YGL246C)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:REG2(YBR050C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease Gene:RGA2(YDR379W)|FD-Score:-4.63|P-value:1.85E-6||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RIM13(YMR154C)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RPL21A(YBR191W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:6.2|P-value:2.78E-10||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RTG2(YGL252C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SDS3(YIL084C)|FD-Score:5.75|P-value:4.50E-9||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SNQ2(YDR011W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPP1(YPL138C)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:SPT10(YJL127C)|FD-Score:4.32|P-value:7.79E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SSZ1(YHR064C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:TSA2(YDR453C)|FD-Score:4.35|P-value:6.83E-6||SGD DESC:Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress Gene:UBP5(YER144C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:UPF3(YGR072W)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:YAP7(YOL028C)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication Gene:YBL094C(YBL094C_d)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YER085C(YER085C_p)|FD-Score:-4.39|P-value:5.62E-6||SGD DESC:Putative protein of unknown function Gene:YER152C(YER152C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YET3(YDL072C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YFT2(YDR319C_p)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR026W(YGR026W_p)|FD-Score:-4.75|P-value:9.96E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGR174W-A(YGR174W-A_p)|FD-Score:-4.39|P-value:5.60E-6||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YGR269W(YGR269W_d)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YHR131C(YHR131C_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YKL169C(YKL169C_d)|FD-Score:5.83|P-value:2.79E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YMR193C-A(YMR193C-A_d)|FD-Score:3.75|P-value:8.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR170W(YOR170W_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Gene:YOR283W(YOR283W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPK3(YBR028C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPQ1(YOL092W_p)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPQ2(YDR352W_p)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter Gene:YUH1(YJR099W)|FD-Score:-5.32|P-value:5.29E-8||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p Gene:ZIP1(YDR285W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ZRT1(YGL255W)|FD-Score:-4.87|P-value:5.47E-7||SGD DESC:High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:ADK2(YER170W)|FD-Score:5.33|P-value:4.83E-8||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ANT1(YPR128C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ATP10(YLR393W)|FD-Score:-4.29|P-value:9.03E-6||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:AUA1(YFL010W-A)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BAP2(YBR068C)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BUD17(YNR027W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:BUD21(YOR078W)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:BUD26(YDR241W_d)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCE1(YKL011C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CDC10(YCR002C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CKI1(YLR133W)|FD-Score:4.58|P-value:2.34E-6||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:COQ2(YNR041C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CRC1(YOR100C)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation Gene:DCI1(YOR180C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DGR2(YKL121W_p)|FD-Score:-7.91|P-value:1.27E-15||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:ELP2(YGR200C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:FCY21(YER060W)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FLO1(YAR050W)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FYV7(YLR068W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:HIS5(YIL116W)|FD-Score:-5.86|P-value:2.34E-9||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IGO2(YHR132W-A)|FD-Score:-3.87|P-value:5.43E-5||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:INP53(YOR109W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:ITR1(YDR497C)|FD-Score:-3.1|P-value:9.79E-4||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:JLP2(YMR132C_p)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KCC4(YCL024W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:LAP3(YNL239W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LDB17(YDL146W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:MAL33(YBR297W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MDV1(YJL112W)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MEC3(YLR288C)|FD-Score:-6.76|P-value:6.78E-12||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MRPL23(YOR150W)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NHA1(YLR138W)|FD-Score:-4.81|P-value:7.40E-7||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:NUP84(YDL116W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PNP1(YLR209C)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PPZ2(YDR436W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PTR2(YKR093W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAI1(YGL246C)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:REG2(YBR050C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease Gene:RGA2(YDR379W)|FD-Score:-4.63|P-value:1.85E-6||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RIM13(YMR154C)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RPL21A(YBR191W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:6.2|P-value:2.78E-10||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RTG2(YGL252C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SDS3(YIL084C)|FD-Score:5.75|P-value:4.50E-9||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SNQ2(YDR011W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPP1(YPL138C)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:SPT10(YJL127C)|FD-Score:4.32|P-value:7.79E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SSZ1(YHR064C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:TSA2(YDR453C)|FD-Score:4.35|P-value:6.83E-6||SGD DESC:Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress Gene:UBP5(YER144C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:UPF3(YGR072W)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:YAP7(YOL028C)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication Gene:YBL094C(YBL094C_d)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YER085C(YER085C_p)|FD-Score:-4.39|P-value:5.62E-6||SGD DESC:Putative protein of unknown function Gene:YER152C(YER152C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YET3(YDL072C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YFT2(YDR319C_p)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR026W(YGR026W_p)|FD-Score:-4.75|P-value:9.96E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGR174W-A(YGR174W-A_p)|FD-Score:-4.39|P-value:5.60E-6||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YGR269W(YGR269W_d)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YHR131C(YHR131C_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YKL169C(YKL169C_d)|FD-Score:5.83|P-value:2.79E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YMR193C-A(YMR193C-A_d)|FD-Score:3.75|P-value:8.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR170W(YOR170W_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Gene:YOR283W(YOR283W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPK3(YBR028C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPQ1(YOL092W_p)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPQ2(YDR352W_p)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter Gene:YUH1(YJR099W)|FD-Score:-5.32|P-value:5.29E-8||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p Gene:ZIP1(YDR285W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ZRT1(YGL255W)|FD-Score:-4.87|P-value:5.47E-7||SGD DESC:High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR102W_d4.641.73E-60.55YOR102W_dDubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
YJL009W_d4.092.11E-50.16YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YNL061W3.934.22E-50.26NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YKL186C3.671.21E-40.31MTR2mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
YDR086C3.373.81E-40.10SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YDR212W3.265.55E-40.05TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YNL110C3.216.71E-40.13NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YNL118C3.070.001060.03DCP2Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
YLR005W3.040.001160.02SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YDR091C3.020.001260.03RLI1Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species
YDR188W2.990.001400.17CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YKL145W2.820.002390.03RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YCL054W2.790.002630.06SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YJL086C_d2.740.003120.04YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YMR146C2.690.003540.01TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL198W6.202.78E-10RPL7BRibosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
YKL169C_d5.832.79E-9YKL169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
YIL084C5.754.50E-9SDS3Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes
YER170W5.334.83E-8ADK2Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background
YPL138C5.161.23E-7SPP1Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein
YKR093W4.621.92E-6PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YLR133W4.582.34E-6CKI1Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
YDR453C4.356.83E-6TSA2Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress
YJL127C4.327.79E-6SPT10Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box
YJL112W3.914.56E-5MDV1Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
YFL010W-A3.885.26E-5AUA1Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease
YCR002C3.855.96E-5CDC10Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress
YOL028C3.836.30E-5YAP7Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication
YOR078W3.816.93E-5BUD21Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
YGL246C3.797.65E-5RAI1Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z

GO enrichment analysis for SGTC_2423
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0681.65E-7SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.0595238translation
0.0656.66E-7SGTC_20422-0095 29.0 μMChemDiv (Drug-like library)67908400.12069redox potentiating
0.0621.69E-6SGTC_25792',5'-dihydroxy-4-methoxychalcone 13.2 μMMicrosource (Natural product library)53558880.112903
0.0612.70E-6SGTC_24315490079 200.0 μMMiscellaneous57223450.112903
0.0604.99E-6SGTC_2727riluzole 51.8 μMMiscellaneous50700.103448mitochondrial processes
0.0561.85E-5SGTC_5181021-0119 62.6 μMChemDiv (Drug-like library)10977620.128571ERG2
0.0552.87E-5SGTC_20955432005 200.0 μMChembridge (Fragment library)7837340.0740741
0.0543.98E-5SGTC_1875nocodazole 6.0 μMMiscellaneous41220.128571tubulin folding & SWR complex
0.0534.57E-5SGTC_22957971645 200.0 μMChembridge (Fragment library)29782260.0877193
0.0534.90E-5SGTC_24245537928 59.2 μMMiscellaneous53828020.0923077
0.0511.09E-4SGTC_7911348-1292 225.0 μMChemDiv (Drug-like library)54370540.146667
0.0501.18E-4SGTC_24345670830 169.9 μMMiscellaneous15498300.0923077
0.0482.20E-4SGTC_22416772625 37.8 μMChembridge (Fragment library)22854110.0454545
0.0482.48E-4SGTC_21956536160 144.2 μMChembridge (Fragment library)38343860.094339660S ribosome export
0.0472.85E-4SGTC_1473k844-0725 134.0 μMChemDiv (Drug-like library)54682906NA

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2612dictamnine15.46 μM0.61538568085Microsource (Natural product library)199.205362.50203RNA pol III & RNase P/MRP
SGTC_12900986-024910.2 μM0.312553329ChemDiv (Drug-like library)300.353844.88613DNA intercalators
SGTC_5171365-0151113 μM0.306122768657ChemDiv (Drug-like library)229.297543.06603
SGTC_2543pimpinellin60.67 μM0.2884624825Microsource (Natural product library)246.21552.1705Golgi
SGTC_1840st05536371 μM0.283019676033TimTec (Natural product derivative library)282.290663.10304
SGTC_2544sphondin68.51 μM0.283019108104Microsource (Natural product library)216.189522.18704RNA pol III & RNase P/MRP
SGTC_15412',3',6-trimethoxyflavone64 μM0.258621688801TimTec (Pure natural product library)312.316643.08705
SGTC_558k064-002721.3 μM0.2545455088526ChemDiv (Drug-like library)334.79895.55113DNA damage response
SGTC_8103825-7150143 μM0.25396822330411ChemDiv (Drug-like library)415.48436.021660S ribosome export
SGTC_11483909-766327.7 μM0.254084526ChemDiv (Drug-like library)320.38833.70205