5628681

N-[(Z)-thiophen-2-ylmethylideneamino]-1,3-benzothiazol-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2425
Screen concentration 7.8 μM
Source Miscellaneous
PubChem CID 5714958
SMILES C1=CC=C2C(=C1)N=C(S2)NN=CC3=CC=CS3
Standardized SMILES N(N=Cc1cccs1)c2nc3ccccc3s2
Molecular weight 259.35
ALogP 4.03
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.45
% growth inhibition (Hom. pool) 10.52


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5714958
Download HIP data (tab-delimited text)  (excel)
Gene:CDC11(YJR076C)|FD-Score:3.72|P-value:9.77E-5|Clearance:0.05||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:GPI11(YDR302W)|FD-Score:4.45|P-value:4.25E-6|Clearance:0.35||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HEM1(YDR232W)|FD-Score:-3.15|P-value:8.29E-4|Clearance:0||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:PDC2(YDR081C)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.12||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:QRI1(YDL103C)|FD-Score:5.98|P-value:1.09E-9|Clearance:0.94||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:SDA1(YGR245C)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.34||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SLD3(YGL113W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:TAF1(YGR274C)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.14||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TEL2(YGR099W)|FD-Score:4.11|P-value:2.02E-5|Clearance:0.12||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TFC1(YBR123C)|FD-Score:5.04|P-value:2.27E-7|Clearance:0.59||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:THI80(YOR143C)|FD-Score:3.98|P-value:3.41E-5|Clearance:0.26||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:TIF35(YDR429C)|FD-Score:-3.37|P-value:3.74E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP20(YBL004W)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:UTP6(YDR449C)|FD-Score:-3.81|P-value:6.90E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:CDC11(YJR076C)|FD-Score:3.72|P-value:9.77E-5|Clearance:0.05||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:GPI11(YDR302W)|FD-Score:4.45|P-value:4.25E-6|Clearance:0.35||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HEM1(YDR232W)|FD-Score:-3.15|P-value:8.29E-4|Clearance:0||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:PDC2(YDR081C)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.12||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:QRI1(YDL103C)|FD-Score:5.98|P-value:1.09E-9|Clearance:0.94||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:SDA1(YGR245C)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.34||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SLD3(YGL113W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:TAF1(YGR274C)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.14||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TEL2(YGR099W)|FD-Score:4.11|P-value:2.02E-5|Clearance:0.12||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TFC1(YBR123C)|FD-Score:5.04|P-value:2.27E-7|Clearance:0.59||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:THI80(YOR143C)|FD-Score:3.98|P-value:3.41E-5|Clearance:0.26||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:TIF35(YDR429C)|FD-Score:-3.37|P-value:3.74E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP20(YBL004W)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:UTP6(YDR449C)|FD-Score:-3.81|P-value:6.90E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5714958
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM17(YHL021C)|FD-Score:6.17|P-value:3.31E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ARE1(YCR048W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:ARG4(YHR018C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ASF1(YJL115W)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:AYR1(YIL124W)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BAP2(YBR068C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BUG1(YDL099W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CBP6(YBR120C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:COG6(YNL041C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ2(YNR041C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:COQ4(YDR204W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:COX23(YHR116W)|FD-Score:3.42|P-value:3.07E-4||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:DAL81(YIR023W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAP2(YHR028C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DDR48(YMR173W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress Gene:EHD3(YDR036C)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:ENT2(YLR206W)|FD-Score:4.57|P-value:2.45E-6||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:ERV41(YML067C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FMT1(YBL013W)|FD-Score:-5.88|P-value:2.10E-9||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FUN26(YAL022C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GTS1(YGL181W)|FD-Score:4.92|P-value:4.23E-7||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:GZF3(YJL110C)|FD-Score:4.31|P-value:8.13E-6||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HUA2(YOR284W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IRC16(YPR038W_d)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:5.7|P-value:5.91E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:ITR1(YDR497C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KAR9(YPL269W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LDB16(YCL005W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MAK31(YCR020C-A)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family Gene:MAK32(YCR019W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MDJ2(YNL328C)|FD-Score:6.43|P-value:6.37E-11||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MDL1(YLR188W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MEP3(YPR138C)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MOT3(YMR070W)|FD-Score:4|P-value:3.20E-5||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRI1(YPR118W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MSB2(YGR014W)|FD-Score:4.28|P-value:9.30E-6||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:NCL1(YBL024W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NOP6(YDL213C)|FD-Score:4.45|P-value:4.26E-6||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:PEX29(YDR479C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX31(YGR004W)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PGD1(YGL025C)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHM6(YDR281C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PRM1(YNL279W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:QRI5(YLR204W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RGS2(YOR107W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:ROM2(YLR371W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL23A(YBL087C)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SCO2(YBR024W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SDS3(YIL084C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SER33(YIL074C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SGF29(YCL010C)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SHB17(YKR043C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SSK1(YLR006C)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:STP2(YHR006W)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SWI5(YDR146C)|FD-Score:5.87|P-value:2.23E-9||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:TVP18(YMR071C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UFD2(YDL190C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VMR1(YHL035C)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VTA1(YLR181C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:WSS1(YHR134W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR012C(YBR012C_d)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YDL057W(YDL057W_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YDL186W(YDL186W_p)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Putative protein of unknown function; YDL186W is not an essential gene Gene:YDL187C(YDL187C_d)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER038W-A(YER038W-A_d)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER068C-A(YER068C-A_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER085C(YER085C_p)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function Gene:YFL042C(YFL042C_p)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL138C(YGL138C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YIL096C(YIL096C_p)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR352W(YLR352W_p)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene Gene:YML053C(YML053C_p)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene Gene:YML079W(YML079W_p)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YML084W(YML084W_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR155W(YMR155W_p)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YOL150C(YOL150C_d)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W(YOR376W_d)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Gene:YPR063C(YPR063C_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:ER-localized protein of unknown function Gene:YUH1(YJR099W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p Gene:ADO1(YJR105W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM17(YHL021C)|FD-Score:6.17|P-value:3.31E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ARE1(YCR048W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:ARG4(YHR018C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ASF1(YJL115W)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:AYR1(YIL124W)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BAP2(YBR068C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BUG1(YDL099W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CBP6(YBR120C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:COG6(YNL041C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ2(YNR041C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:COQ4(YDR204W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:COX23(YHR116W)|FD-Score:3.42|P-value:3.07E-4||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:DAL81(YIR023W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAP2(YHR028C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DDR48(YMR173W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress Gene:EHD3(YDR036C)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:ENT2(YLR206W)|FD-Score:4.57|P-value:2.45E-6||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:ERV41(YML067C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FMT1(YBL013W)|FD-Score:-5.88|P-value:2.10E-9||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FUN26(YAL022C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GTS1(YGL181W)|FD-Score:4.92|P-value:4.23E-7||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:GZF3(YJL110C)|FD-Score:4.31|P-value:8.13E-6||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HUA2(YOR284W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IRC16(YPR038W_d)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:5.7|P-value:5.91E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:ITR1(YDR497C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KAR9(YPL269W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LDB16(YCL005W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MAK31(YCR020C-A)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family Gene:MAK32(YCR019W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MDJ2(YNL328C)|FD-Score:6.43|P-value:6.37E-11||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MDL1(YLR188W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MEP3(YPR138C)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MOT3(YMR070W)|FD-Score:4|P-value:3.20E-5||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRI1(YPR118W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MSB2(YGR014W)|FD-Score:4.28|P-value:9.30E-6||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:NCL1(YBL024W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NOP6(YDL213C)|FD-Score:4.45|P-value:4.26E-6||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:PEX29(YDR479C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX31(YGR004W)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PGD1(YGL025C)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHM6(YDR281C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PRM1(YNL279W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:QRI5(YLR204W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RGS2(YOR107W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:ROM2(YLR371W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL23A(YBL087C)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SCO2(YBR024W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SDS3(YIL084C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SER33(YIL074C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SGF29(YCL010C)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SHB17(YKR043C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SSK1(YLR006C)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:STP2(YHR006W)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SWI5(YDR146C)|FD-Score:5.87|P-value:2.23E-9||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:TVP18(YMR071C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UFD2(YDL190C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VMR1(YHL035C)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VTA1(YLR181C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:WSS1(YHR134W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR012C(YBR012C_d)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YDL057W(YDL057W_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YDL186W(YDL186W_p)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Putative protein of unknown function; YDL186W is not an essential gene Gene:YDL187C(YDL187C_d)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER038W-A(YER038W-A_d)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER068C-A(YER068C-A_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER085C(YER085C_p)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function Gene:YFL042C(YFL042C_p)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL138C(YGL138C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YIL096C(YIL096C_p)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR352W(YLR352W_p)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene Gene:YML053C(YML053C_p)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene Gene:YML079W(YML079W_p)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YML084W(YML084W_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR155W(YMR155W_p)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YOL150C(YOL150C_d)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W(YOR376W_d)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Gene:YPR063C(YPR063C_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:ER-localized protein of unknown function Gene:YUH1(YJR099W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL103C5.981.09E-90.94QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YBR123C5.042.27E-70.59TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YDR302W4.454.25E-60.35GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YGR099W4.112.02E-50.12TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YOR143C3.983.41E-50.26THI80Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)
YJR076C3.729.77E-50.05CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YDR081C3.671.21E-40.12PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YGR274C3.551.92E-40.14TAF1TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression
YGR245C3.413.24E-40.34SDA1Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
YGL116W3.070.001060.05CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YBR060C3.020.001260.03ORC2Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
YOL135C2.990.001410.07MED7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YER125W2.920.001750.05RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
YJL173C2.870.002040.12RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YOR335C2.750.002980.02ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL328C6.436.37E-11MDJ2Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain
YHL021C6.173.31E-10AIM17Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
YDR146C5.872.23E-9SWI5Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
YKR019C5.705.91E-9IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YGL181W4.924.23E-7GTS1Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
YPL269W4.751.03E-6KAR9Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
YDL186W_p4.701.31E-6YDL186W_pPutative protein of unknown function; YDL186W is not an essential gene
YHR006W4.661.58E-6STP2Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
YLR206W4.572.45E-6ENT2Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus
YJR099W4.533.00E-6YUH1Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
YDL213C4.454.26E-6NOP6rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes
YJL110C4.318.13E-6GZF3GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
YGR004W4.289.29E-6PEX31Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p
YGR014W4.289.30E-6MSB2Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate
YOL150C_d4.231.18E-5YOL150C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2425
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0993.08E-14SGTC_24275545055 42.8 μMMiscellaneous53728580.296296
0.0921.38E-12SGTC_24605552655 174.2 μMMiscellaneous54019890.180328amide catabolism
0.0863.80E-11SGTC_32929120825 59.8 μMChembridge (Drug-like library)248249000.119403
0.0864.10E-11SGTC_2680butyl paraben 22.2 μMMicrosource (Natural product library)71840.0491803amide catabolism
0.0831.57E-10SGTC_486niguldipine 82.0 μMMiscellaneous12360.0515464amide catabolism
0.0764.29E-9SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.136986Golgi
0.0765.33E-9SGTC_24295902844 64.4 μMMiscellaneous19277900.0769231
0.0741.14E-8SGTC_32289131799 49.5 μMChembridge (Drug-like library)170158180.0958904amide catabolism
0.0723.15E-8SGTC_22117117706 200.0 μMChembridge (Fragment library)8227710.105263
0.0681.47E-7SGTC_15494'-methoxyflavonol 74.6 μMTimTec (Pure natural product library)971410.0757576
0.0681.91E-7SGTC_1751st044839 27.2 μMTimTec (Natural product derivative library)52913260.0379747Golgi
0.0672.61E-7SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.0967742
0.0664.06E-7SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.0714286amide catabolism
0.0654.90E-7SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.162162amide catabolism
0.0655.19E-7SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.103896PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_772k072-017235.3 μM0.6521745861980ChemDiv (Drug-like library)338.246024.77615
SGTC_623k072-010816.9 μM0.6136365382803ChemDiv (Drug-like library)254.31032.92315heme biosynthesis & mitochondrial translocase
SGTC_24315490079200 μM0.65722345Miscellaneous267.348824.5614
SGTC_243656335228.09 μM0.65724242Miscellaneous254.31033.35215
SGTC_24405628896157.78 μM0.65522048Miscellaneous271.3127034.27915
SGTC_24435493264126.67 μM0.65340055Miscellaneous269.321642.39725
SGTC_2444548548436.41 μM0.66790631Miscellaneous269.321642.39725
SGTC_2432563086412.22 μM0.5869575714970Miscellaneous243.284363.46915
SGTC_2424553792859.2 μM0.5744685382802Miscellaneous254.31032.92315
SGTC_1883229-0966278.47 μM0.56255722370ChemDiv (Drug-like library)269.321643.83225