5582046

methyl 4-[[(5E)-5-[(2-fluorophenyl)methylidene]-2,4-dioxo-1,3-thiazolidin-3-yl]methylamino]benzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2426
Screen concentration 32.2 μM
Source Miscellaneous
PubChem CID 2251934
SMILES COC(=O)C1=CC=C(C=C1)NCN2C(=O)C(=CC3=CC=CC=C3F)SC2=O
Standardized SMILES COC(=O)c1ccc(NCN2C(=O)SC(=Cc3ccccc3F)C2=O)cc1
Molecular weight 386.3968
ALogP 3.65
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.65
% growth inhibition (Hom. pool) 4.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2251934
Download HIP data (tab-delimited text)  (excel)
Gene:CDC24(YAL041W)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.42||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DPB11(YJL090C)|FD-Score:4.9|P-value:4.84E-7|Clearance:0.57||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:EMW1(YNL313C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.02||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:FRS2(YFL022C)|FD-Score:-3.76|P-value:8.60E-5|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:LCB1(YMR296C)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.16||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MEX67(YPL169C)|FD-Score:4.27|P-value:9.78E-6|Clearance:0.01||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:RET3(YPL010W)|FD-Score:6.71|P-value:9.69E-12|Clearance:1.81||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPL5(YPL131W)|FD-Score:4.08|P-value:2.29E-5|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEC27(YGL137W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SPC3(YLR066W)|FD-Score:4.33|P-value:7.58E-6|Clearance:0.06||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:TAF5(YBR198C)|FD-Score:4.04|P-value:2.63E-5|Clearance:0.36||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:UTP14(YML093W)|FD-Score:-3.92|P-value:4.51E-5|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP9(YHR196W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.24||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YLR317W(YLR317W_d)|FD-Score:-3.27|P-value:5.33E-4|Clearance:0||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:CDC24(YAL041W)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.42||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DPB11(YJL090C)|FD-Score:4.9|P-value:4.84E-7|Clearance:0.57||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:EMW1(YNL313C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.02||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:FRS2(YFL022C)|FD-Score:-3.76|P-value:8.60E-5|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:LCB1(YMR296C)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.16||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MEX67(YPL169C)|FD-Score:4.27|P-value:9.78E-6|Clearance:0.01||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:RET3(YPL010W)|FD-Score:6.71|P-value:9.69E-12|Clearance:1.81||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPL5(YPL131W)|FD-Score:4.08|P-value:2.29E-5|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEC27(YGL137W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SPC3(YLR066W)|FD-Score:4.33|P-value:7.58E-6|Clearance:0.06||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:TAF5(YBR198C)|FD-Score:4.04|P-value:2.63E-5|Clearance:0.36||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:UTP14(YML093W)|FD-Score:-3.92|P-value:4.51E-5|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP9(YHR196W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.24||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YLR317W(YLR317W_d)|FD-Score:-3.27|P-value:5.33E-4|Clearance:0||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2251934
Download HOP data (tab-delimited text)  (excel)
Gene:ALF1(YNL148C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ASM4(YDL088C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATP3(YBR039W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP5(YDR298C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BDF1(YLR399C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BRP1(YGL007W_d)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CHS7(YHR142W)|FD-Score:4.4|P-value:5.29E-6||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CSM4(YPL200W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CYC7(YEL039C)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DCI1(YOR180C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DIN7(YDR263C)|FD-Score:4.95|P-value:3.74E-7||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:ECL1(YGR146C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EHT1(YBR177C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ERP2(YAL007C)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FAR8(YMR029C)|FD-Score:5.57|P-value:1.25E-8||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FOX2(YKR009C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GET3(YDL100C)|FD-Score:4.76|P-value:9.61E-7||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GIP1(YBR045C)|FD-Score:-5.39|P-value:3.60E-8||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GOT1(YMR292W)|FD-Score:7.12|P-value:5.51E-13||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GTS1(YGL181W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HMG2(YLR450W)|FD-Score:-4.14|P-value:1.70E-5||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:HNT1(YDL125C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:LAC1(YKL008C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:MEF2(YJL102W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MEH1(YKR007W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MFB1(YDR219C)|FD-Score:4.99|P-value:3.02E-7||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MKC7(YDR144C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MRPS8(YMR158W)|FD-Score:4.37|P-value:6.31E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRS1(YIR021W)|FD-Score:9.69|P-value:1.62E-22||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:NUP133(YKR082W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:NUP170(YBL079W)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OAC1(YKL120W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OAZ1(YPL052W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:OPI11(YPR044C_d)|FD-Score:-3.33|P-value:4.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:OTU2(YHL013C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PER1(YCR044C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET191(YJR034W)|FD-Score:7.16|P-value:3.98E-13||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PFA4(YOL003C)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases Gene:PHO81(YGR233C)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PMC1(YGL006W)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:PRM5(YIL117C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:RAV2(YDR202C)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RPL11B(YGR085C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL18B(YNL301C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPS14B(YJL191W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SBH1(YER087C-B)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEC28(YIL076W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SFB3(YHR098C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHE3(YBR130C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SMI1(YGR229C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPE3(YPR069C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SRO7(YPR032W)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SUL1(YBR294W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SYM1(YLR251W)|FD-Score:5.25|P-value:7.79E-8||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:SYS1(YJL004C)|FD-Score:4.03|P-value:2.85E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:UGA3(YDL170W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:YBR226C(YBR226C_d)|FD-Score:-5.24|P-value:7.91E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCR095W-A(YCR095W-A_p)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Putative protein of unknown function Gene:YDL187C(YDL187C_d)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR248C(YDR248C_p)|FD-Score:5.4|P-value:3.30E-8||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YDR541C(YDR541C_p)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YEN1(YER041W)|FD-Score:-4.51|P-value:3.29E-6||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER187W(YER187W_p)|FD-Score:4.29|P-value:9.09E-6||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YIR024C(YIR024C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YIR035C(YIR035C_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKL053W(YKL053W_d)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YKR040C(YKR040C_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YKR041W(YKR041W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YKR045C(YKR045C_p)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR108C(YLR108C_p)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YLR361C-A(YLR361C-A_p)|FD-Score:5.93|P-value:1.50E-9||SGD DESC:Putative protein of unknown function Gene:YMR124W(YMR124W_p)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR155W(YMR155W_p)|FD-Score:7.88|P-value:1.61E-15||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR279C(YMR279C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YOR292C(YOR292C_p)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:YPL073C(YPL073C_d)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:ALF1(YNL148C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ASM4(YDL088C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATP3(YBR039W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP5(YDR298C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BDF1(YLR399C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BRP1(YGL007W_d)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CHS7(YHR142W)|FD-Score:4.4|P-value:5.29E-6||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CSM4(YPL200W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CYC7(YEL039C)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DCI1(YOR180C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DIN7(YDR263C)|FD-Score:4.95|P-value:3.74E-7||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:ECL1(YGR146C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EHT1(YBR177C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ERP2(YAL007C)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FAR8(YMR029C)|FD-Score:5.57|P-value:1.25E-8||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FOX2(YKR009C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GET3(YDL100C)|FD-Score:4.76|P-value:9.61E-7||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GIP1(YBR045C)|FD-Score:-5.39|P-value:3.60E-8||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GOT1(YMR292W)|FD-Score:7.12|P-value:5.51E-13||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GTS1(YGL181W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HMG2(YLR450W)|FD-Score:-4.14|P-value:1.70E-5||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:HNT1(YDL125C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:LAC1(YKL008C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:MEF2(YJL102W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MEH1(YKR007W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MFB1(YDR219C)|FD-Score:4.99|P-value:3.02E-7||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MKC7(YDR144C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MRPS8(YMR158W)|FD-Score:4.37|P-value:6.31E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRS1(YIR021W)|FD-Score:9.69|P-value:1.62E-22||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:NUP133(YKR082W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:NUP170(YBL079W)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OAC1(YKL120W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OAZ1(YPL052W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:OPI11(YPR044C_d)|FD-Score:-3.33|P-value:4.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:OTU2(YHL013C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PER1(YCR044C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET191(YJR034W)|FD-Score:7.16|P-value:3.98E-13||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PFA4(YOL003C)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases Gene:PHO81(YGR233C)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PMC1(YGL006W)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:PRM5(YIL117C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:RAV2(YDR202C)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RPL11B(YGR085C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL18B(YNL301C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPS14B(YJL191W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SBH1(YER087C-B)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEC28(YIL076W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SFB3(YHR098C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHE3(YBR130C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SMI1(YGR229C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPE3(YPR069C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SRO7(YPR032W)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SUL1(YBR294W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SYM1(YLR251W)|FD-Score:5.25|P-value:7.79E-8||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:SYS1(YJL004C)|FD-Score:4.03|P-value:2.85E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:UGA3(YDL170W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:YBR226C(YBR226C_d)|FD-Score:-5.24|P-value:7.91E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCR095W-A(YCR095W-A_p)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Putative protein of unknown function Gene:YDL187C(YDL187C_d)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR248C(YDR248C_p)|FD-Score:5.4|P-value:3.30E-8||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YDR541C(YDR541C_p)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YEN1(YER041W)|FD-Score:-4.51|P-value:3.29E-6||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER187W(YER187W_p)|FD-Score:4.29|P-value:9.09E-6||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YIR024C(YIR024C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YIR035C(YIR035C_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKL053W(YKL053W_d)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YKR040C(YKR040C_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YKR041W(YKR041W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YKR045C(YKR045C_p)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR108C(YLR108C_p)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YLR361C-A(YLR361C-A_p)|FD-Score:5.93|P-value:1.50E-9||SGD DESC:Putative protein of unknown function Gene:YMR124W(YMR124W_p)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR155W(YMR155W_p)|FD-Score:7.88|P-value:1.61E-15||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR279C(YMR279C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YOR292C(YOR292C_p)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:YPL073C(YPL073C_d)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL010W6.719.69E-121.81RET3Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YJL090C4.904.84E-70.57DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YLR066W4.337.58E-60.06SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YPL169C4.279.78E-60.01MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YNL313C4.261.03E-50.02EMW1Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress
YMR296C4.241.12E-50.16LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YPL131W4.082.29E-50.03RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YBR198C4.042.63E-50.36TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YAL041W3.691.14E-40.42CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YHR196W3.275.42E-40.24UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGL098W3.030.001220.04USE1Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p
YDL205C2.990.001380.02HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YER136W2.980.001450.15GDI1GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
YBL076C2.820.002370.07ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YJL091C2.760.002920.02GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR021W9.691.62E-22MRS1Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA
YMR155W_p7.881.61E-15YMR155W_pPutative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen
YJR034W7.163.98E-13PET191Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery
YMR292W7.125.51E-13GOT1Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition
YLR361C-A_p5.931.50E-9YLR361C-A_pPutative protein of unknown function
YMR029C5.571.25E-8FAR8Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
YDR248C_p5.403.30E-8YDR248C_pPutative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
YLR251W5.257.79E-8SYM1Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17
YDR202C5.131.47E-7RAV2Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
YDR219C4.993.02E-7MFB1Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding
YDR263C4.953.74E-7DIN7Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination
YDL100C4.769.61E-7GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
YMR279C4.741.05E-6YMR279CPutative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication
YNL148C4.582.36E-6ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YLR108C_p4.434.66E-6YLR108C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2426
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0741.21E-8SGTC_1534077-0274 519.7 μMChemDiv (Drug-like library)31219760.0729167
0.0603.87E-6SGTC_204PMA1.184 62.4 μMChemDiv (Drug-like library)22522130.571429TRP & mitochondrial translation
0.0552.60E-5SGTC_1704st028805 70.8 μMTimTec (Natural product derivative library)28761010.111111
0.0543.53E-5SGTC_8920929-0076 29.0 μMChemDiv (Drug-like library)57510380.161765TRP & mitochondrial translation
0.0535.32E-5SGTC_5931203-0218 61.7 μMChemDiv (Drug-like library)36669560.148649
0.0464.00E-4SGTC_3071501-1223 22.3 μMChemDiv (Drug-like library)28303290.125
0.0455.31E-4SGTC_12961068-0102 11.9 μMChemDiv (Drug-like library)7670530.175676
0.0447.41E-4SGTC_7381315-0003 72.4 μMChemDiv (Drug-like library)62514740.0967742
0.0447.56E-4SGTC_7593486-0358 89.3 μMChemDiv (Drug-like library)8751030.103448
0.0447.65E-4SGTC_19215740136 50.0 μMMiscellaneous17742430.347222TRP & mitochondrial translation
0.0439.46E-4SGTC_1987st072290 61.9 μMTimTec (Natural product derivative library)54697379NA
0.0420.00110SGTC_23157733312 200.0 μMChembridge (Fragment library)7263160.257576
0.0420.00120SGTC_21465545642 200.0 μMChembridge (Fragment library)8253260.0779221
0.0410.00153SGTC_6710560-0003 407.0 μMChemDiv (Drug-like library)32386780.0875
0.0410.00167SGTC_13191306-0155 43.7 μMChemDiv (Drug-like library)31223600.102564

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1922557818725 μM0.5833331350412Miscellaneous362.8057834.45715TRP & mitochondrial translation
SGTC_2041543-018462.41 μM0.5714292252213ChemDiv (Drug-like library)356.4138834.76515TRP & mitochondrial translation
SGTC_2441573094639.09 μM0.5217391774275Miscellaneous461.328984.67716
SGTC_2450573973712.93 μM0.4918032262825Miscellaneous328.3607233.79315
SGTC_2453573113222.18 μM0.4918031362040Miscellaneous436.266794.16614
SGTC_2433560787427.82 μM0.4848482252428Miscellaneous374.84134.23515
SGTC_24375741531144.16 μM0.3918922262990Miscellaneous402.4392634.24617
SGTC_8191013-025827.7 μM0.3802825753198ChemDiv (Drug-like library)390.90695.13415
SGTC_2445574259735.57 μM0.3684211774255Miscellaneous433.318884.80515
SGTC_2447574174233.78 μM0.3648651776504Miscellaneous433.318884.80515