5706476

4-[[2-(1,3-benzothiazol-2-yl)hydrazinyl]methylidene]-2,6-dimethylcyclohexa-2,5-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2435
Screen concentration 10.6 μM
Source Miscellaneous
PubChem CID 5725375
SMILES CC1=CC(=CNNC2=NC3=CC=CC=C3S2)C=C(C1=O)C
Standardized SMILES Cc1cc(C=NNc2nc3ccccc3s2)cc(C)c1O
Molecular weight 297.3748
ALogP 3.29
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.89
% growth inhibition (Hom. pool) 8.21


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5725375
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.05||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CBK1(YNL161W)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.35||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:EBP2(YKL172W)|FD-Score:-3.99|P-value:3.36E-5|Clearance:0||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:FHL1(YPR104C)|FD-Score:-3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:IDI1(YPL117C)|FD-Score:4.52|P-value:3.05E-6|Clearance:0.78||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:NMT1(YLR195C)|FD-Score:4.3|P-value:8.40E-6|Clearance:0.78||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:PRE3(YJL001W)|FD-Score:-3.75|P-value:8.89E-5|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PUP3(YER094C)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:SMC1(YFL008W)|FD-Score:-3.83|P-value:6.32E-5|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TFC1(YBR123C)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.04||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:ULP2(YIL031W)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:ABF1(YKL112W)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.05||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CBK1(YNL161W)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.35||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:EBP2(YKL172W)|FD-Score:-3.99|P-value:3.36E-5|Clearance:0||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:FHL1(YPR104C)|FD-Score:-3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:IDI1(YPL117C)|FD-Score:4.52|P-value:3.05E-6|Clearance:0.78||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:NMT1(YLR195C)|FD-Score:4.3|P-value:8.40E-6|Clearance:0.78||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:PRE3(YJL001W)|FD-Score:-3.75|P-value:8.89E-5|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PUP3(YER094C)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:SMC1(YFL008W)|FD-Score:-3.83|P-value:6.32E-5|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TFC1(YBR123C)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.04||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:ULP2(YIL031W)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5725375
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AAP1(YHR047C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ACA1(YER045C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:AIM23(YJL131C)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss Gene:CDA1(YLR307W)|FD-Score:5.69|P-value:6.47E-9||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CNM67(YNL225C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:6.63|P-value:1.73E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRG1(YHR209W)|FD-Score:4.84|P-value:6.37E-7||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DAK1(YML070W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DAL81(YIR023W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAL82(YNL314W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DBP3(YGL078C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DSF2(YBR007C_p)|FD-Score:4.92|P-value:4.33E-7||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EMC5(YIL027C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:EMC6(YLL014W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:FRT1(YOR324C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GAP1(YKR039W)|FD-Score:-3.79|P-value:7.65E-5||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GPA2(YER020W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GRE3(YHR104W)|FD-Score:4.76|P-value:9.70E-7||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HOG1(YLR113W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:ICS3(YJL077C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IML3(YBR107C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:JJJ1(YNL227C)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:MDJ2(YNL328C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MIG2(YGL209W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MOD5(YOR274W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MPA43(YNL249C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MPM1(YJL066C)|FD-Score:6.19|P-value:2.96E-10||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MSF1(YPR047W)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MYO3(YKL129C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:NAM7(YMR080C)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NHP10(YDL002C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NMA1(YLR328W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH3(YHR073W)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OTU1(YFL044C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PAM17(YKR065C)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:7.02|P-value:1.10E-12||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX31(YGR004W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PPN1(YDR452W)|FD-Score:-5.65|P-value:8.05E-9||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PSP2(YML017W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:RAX2(YLR084C)|FD-Score:-4.55|P-value:2.74E-6||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RPL29(YFR032C-A)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL2B(YIL018W)|FD-Score:4.77|P-value:9.05E-7||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPN4(YDL020C)|FD-Score:4.77|P-value:8.99E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RTA1(YGR213C)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SCS2(YER120W)|FD-Score:4.33|P-value:7.40E-6||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SDH4(YDR178W)|FD-Score:-3.84|P-value:6.24E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SGE1(YPR198W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHH3(YMR118C_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SLT2(YHR030C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF12(YNR023W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNO2(YNL334C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SPG1(YGR236C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPS22(YCL048W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall Gene:SPT3(YDR392W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SST2(YLR452C)|FD-Score:6.41|P-value:7.31E-11||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:TAE1(YBR261C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TAT2(YOL020W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TDP1(YBR223C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TSC3(YBR058C-A)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA13(YPR036W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:7.65|P-value:9.89E-15||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:WSS1(YHR134W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YDR442W(YDR442W_d)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR219W(YGR219W_d)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHR131C(YHR131C_p)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YJL175W(YJL175W_d)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YMR124W(YMR124W_p)|FD-Score:3.9|P-value:4.91E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YPL014W(YPL014W_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL041C(YPL041C_p)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPR022C(YPR022C_p)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YRB30(YGL164C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:YVC1(YOR087W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock Gene:ZRG8(YER033C)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ZWF1(YNL241C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AAD3(YCR107W)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AAP1(YHR047C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ACA1(YER045C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:AIM23(YJL131C)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss Gene:CDA1(YLR307W)|FD-Score:5.69|P-value:6.47E-9||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CNM67(YNL225C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:6.63|P-value:1.73E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRG1(YHR209W)|FD-Score:4.84|P-value:6.37E-7||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DAK1(YML070W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DAL81(YIR023W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAL82(YNL314W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DBP3(YGL078C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DSF2(YBR007C_p)|FD-Score:4.92|P-value:4.33E-7||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EMC5(YIL027C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:EMC6(YLL014W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:FRT1(YOR324C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GAP1(YKR039W)|FD-Score:-3.79|P-value:7.65E-5||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GPA2(YER020W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GRE3(YHR104W)|FD-Score:4.76|P-value:9.70E-7||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HOG1(YLR113W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:ICS3(YJL077C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IML3(YBR107C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:JJJ1(YNL227C)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:MDJ2(YNL328C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MIG2(YGL209W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MOD5(YOR274W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MPA43(YNL249C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MPM1(YJL066C)|FD-Score:6.19|P-value:2.96E-10||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MSF1(YPR047W)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MYO3(YKL129C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:NAM7(YMR080C)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NHP10(YDL002C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NMA1(YLR328W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH3(YHR073W)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OTU1(YFL044C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PAM17(YKR065C)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:7.02|P-value:1.10E-12||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX31(YGR004W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PPN1(YDR452W)|FD-Score:-5.65|P-value:8.05E-9||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PSP2(YML017W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:RAX2(YLR084C)|FD-Score:-4.55|P-value:2.74E-6||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RPL29(YFR032C-A)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL2B(YIL018W)|FD-Score:4.77|P-value:9.05E-7||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPN4(YDL020C)|FD-Score:4.77|P-value:8.99E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RTA1(YGR213C)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SCS2(YER120W)|FD-Score:4.33|P-value:7.40E-6||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SDH4(YDR178W)|FD-Score:-3.84|P-value:6.24E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SGE1(YPR198W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHH3(YMR118C_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SLT2(YHR030C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF12(YNR023W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNO2(YNL334C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SPG1(YGR236C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPS22(YCL048W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall Gene:SPT3(YDR392W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SST2(YLR452C)|FD-Score:6.41|P-value:7.31E-11||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:TAE1(YBR261C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TAT2(YOL020W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TDP1(YBR223C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TSC3(YBR058C-A)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA13(YPR036W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:7.65|P-value:9.89E-15||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:WSS1(YHR134W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YDR442W(YDR442W_d)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR219W(YGR219W_d)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHR131C(YHR131C_p)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YJL175W(YJL175W_d)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YMR124W(YMR124W_p)|FD-Score:3.9|P-value:4.91E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YPL014W(YPL014W_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL041C(YPL041C_p)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPR022C(YPR022C_p)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YRB30(YGL164C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:YVC1(YOR087W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock Gene:ZRG8(YER033C)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ZWF1(YNL241C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YPL117C4.523.05E-60.78IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
YLR195C4.308.40E-60.78NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YBR123C3.532.10E-40.04TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YKL112W3.492.46E-40.05ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YNL161W3.432.98E-40.35CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YNL313C3.080.001030.06EMW1Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress
YLL035W3.020.001240.03GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
YNL245C2.990.001390.09CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YBR198C2.900.001890.05TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YOR250C2.840.002230.00CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YMR203W2.840.002260.04TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YOR098C2.800.002550.01NUP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
YLL050C2.790.002660.01COF1Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
YKL013C2.770.002770.02ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YMR290C2.760.002930.05HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR020C7.659.89E-15VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YGL013C7.021.10E-12PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YGL005C6.631.73E-11COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR452C6.417.31E-11SST2GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
YJL066C6.192.96E-10MPM1Mitochondrial intermembrane space protein of unknown function
YLR307W5.696.47E-9CDA1Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
YHR073W5.171.15E-7OSH3Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
YKR065C5.062.11E-7PAM17Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress
YBR007C_p4.924.33E-7DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YBR058C-A4.856.12E-7TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YHR209W4.846.37E-7CRG1S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
YDL020C4.778.99E-7RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YIL018W4.779.05E-7RPL2BRibosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures
YHR104W4.769.70E-7GRE3Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
YHR204W4.741.08E-6MNL1Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation

GO enrichment analysis for SGTC_2435
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1803.95E-44SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.15625TSC3-RPN4
0.1736.28E-41SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.114943PDR1
0.1668.65E-38SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.084507PDR1
0.1505.59E-31SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0638298PDR1
0.1483.18E-30SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0681818PDR1
0.1455.64E-29SGTC_24305571206 200.0 μMMiscellaneous17317710.0789474
0.1449.46E-29SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.15
0.1441.11E-28SGTC_389tpck 16.7 μMMiscellaneous4396470.112676
0.1433.34E-28SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.15
0.1426.59E-28SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0441176TSC3-RPN4
0.1411.64E-27SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.155844PDR1
0.1402.77E-27SGTC_2740felodipine 17.1 μMMiscellaneous33330.0779221
0.1381.93E-26SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0869565
0.1352.36E-25SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0821918PDR1
0.1343.66E-25SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.106061TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_24435493264126.67 μM0.6888895340055Miscellaneous269.321642.39725
SGTC_1883229-0966278.47 μM0.6808515722370ChemDiv (Drug-like library)269.321643.83225
SGTC_243954920307.2 μM0.6808515722370Miscellaneous269.321643.83225
SGTC_3823229-096623.2 μM0.6808515722370ChemDiv (Drug-like library)269.321643.83225
SGTC_24315490079200 μM0.6521745722345Miscellaneous267.348824.5614
SGTC_286k060-006722.9 μM0.6346155985051ChemDiv (Drug-like library)299.347623.81526tubulin folding & SWR complex
SGTC_623k072-010816.9 μM0.6304355382803ChemDiv (Drug-like library)254.31032.92315heme biosynthesis & mitochondrial translocase
SGTC_2444548548436.41 μM0.6170216790631Miscellaneous269.321642.39725
SGTC_2424553792859.2 μM0.5918375382802Miscellaneous254.31032.92315
SGTC_242556286817.85 μM0.551025714958Miscellaneous259.349964.02715