nsc-207895

4-(4-methylpiperazin-1-yl)-7-nitro-3-oxido-2,1,3-benzoxadiazol-3-ium

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_244
Screen concentration 1.3 μM
Source Miscellaneous
PubChem CID 42640
SMILES CN1CCN(CC1)C2=CC=C(C3=NO[N+](=C23)[O-])[N+](=O)[O-]
Standardized SMILES CN1CCN(CC1)c2ccc(c3no[n+]([O-])c23)[N+](=O)[O-]
Molecular weight 279.252
ALogP -1.28
H-bond donor count 0
H-bond acceptor count 7
Response signature DNA damage response

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.4
% growth inhibition (Hom. pool) 5.97


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 42640
Download HIP data (tab-delimited text)  (excel)
Gene:HIP1(YGR191W)|FD-Score:3.94|P-value:4.16E-5|Clearance:0.99||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:MET30(YIL046W)|FD-Score:-3.15|P-value:8.15E-4|Clearance:0||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:PRE6(YOL038W)|FD-Score:-3.16|P-value:7.99E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RFT1(YBL020W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPP1(YHR062C)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:HIP1(YGR191W)|FD-Score:3.94|P-value:4.16E-5|Clearance:0.99||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:MET30(YIL046W)|FD-Score:-3.15|P-value:8.15E-4|Clearance:0||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:PRE6(YOL038W)|FD-Score:-3.16|P-value:7.99E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RFT1(YBL020W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPP1(YHR062C)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 42640
Download HOP data (tab-delimited text)  (excel)
Gene:AIM17(YHL021C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALB1(YJL122W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:BCK1(YJL095W)|FD-Score:5.86|P-value:2.27E-9||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM3(YPL115C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:COS111(YBR203W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DSD1(YGL196W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ECM2(YBR065C)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ENT5(YDR153C)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:ERV46(YAL042W)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:ESC2(YDR363W)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:HFM1(YGL251C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:ICS3(YJL077C)|FD-Score:-4.8|P-value:7.89E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:ISW2(YOR304W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth Gene:KEL2(YGR238C)|FD-Score:-4.86|P-value:5.76E-7||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LDB7(YBL006C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LST7(YGR057C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MMS4(YBR098W)|FD-Score:13.4|P-value:2.40E-41||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MSW1(YDR268W)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MUS81(YDR386W)|FD-Score:15.2|P-value:1.94E-52||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:OMS1(YDR316W)|FD-Score:5.01|P-value:2.74E-7||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PHO89(YBR296C)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIB1(YDR313C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PLB1(YMR008C)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PSK1(YAL017W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:RAD18(YCR066W)|FD-Score:4.3|P-value:8.48E-6||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:4.33|P-value:7.51E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD50(YNL250W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD51(YER095W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD54(YGL163C)|FD-Score:9.78|P-value:6.80E-23||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:6.06|P-value:6.70E-10||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:7.59|P-value:1.62E-14||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:6.51|P-value:3.79E-11||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RTT101(YJL047C)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAE2(YGL175C)|FD-Score:6.83|P-value:4.40E-12||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAS5(YOR213C)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SHU2(YDR078C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SMF3(YLR034C)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SRS2(YJL092W)|FD-Score:8.73|P-value:1.31E-18||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:TRS85(YDR108W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:WHI2(YOR043W)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:XRS2(YDR369C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBL055C(YBL055C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR099C(YBR099C_d)|FD-Score:10.9|P-value:3.35E-28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YFR018C(YFR018C_p)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function Gene:YHR182W(YHR182W_p)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YLR012C(YLR012C_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YOR345C(YOR345C_d)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPL067C(YPL067C_p)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:AIM17(YHL021C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALB1(YJL122W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:BCK1(YJL095W)|FD-Score:5.86|P-value:2.27E-9||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM3(YPL115C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:COS111(YBR203W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DSD1(YGL196W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ECM2(YBR065C)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ENT5(YDR153C)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:ERV46(YAL042W)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:ESC2(YDR363W)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:HFM1(YGL251C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:ICS3(YJL077C)|FD-Score:-4.8|P-value:7.89E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:ISW2(YOR304W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth Gene:KEL2(YGR238C)|FD-Score:-4.86|P-value:5.76E-7||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LDB7(YBL006C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LST7(YGR057C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MMS4(YBR098W)|FD-Score:13.4|P-value:2.40E-41||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MSW1(YDR268W)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MUS81(YDR386W)|FD-Score:15.2|P-value:1.94E-52||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:OMS1(YDR316W)|FD-Score:5.01|P-value:2.74E-7||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PHO89(YBR296C)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIB1(YDR313C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PLB1(YMR008C)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PSK1(YAL017W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:RAD18(YCR066W)|FD-Score:4.3|P-value:8.48E-6||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:4.33|P-value:7.51E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD50(YNL250W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD51(YER095W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD54(YGL163C)|FD-Score:9.78|P-value:6.80E-23||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:6.06|P-value:6.70E-10||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:7.59|P-value:1.62E-14||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:6.51|P-value:3.79E-11||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RTT101(YJL047C)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAE2(YGL175C)|FD-Score:6.83|P-value:4.40E-12||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAS5(YOR213C)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SHU2(YDR078C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SMF3(YLR034C)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SRS2(YJL092W)|FD-Score:8.73|P-value:1.31E-18||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:TRS85(YDR108W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:WHI2(YOR043W)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:XRS2(YDR369C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBL055C(YBL055C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR099C(YBR099C_d)|FD-Score:10.9|P-value:3.35E-28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YFR018C(YFR018C_p)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function Gene:YHR182W(YHR182W_p)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YLR012C(YLR012C_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YOR345C(YOR345C_d)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPL067C(YPL067C_p)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR191W3.944.16E-50.99HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YLR005W2.950.001600.10SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YPL228W2.850.002170.01CET1Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YDR182W2.840.002240.02CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
YMR146C2.830.002360.28TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YPL122C2.550.005400.09TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YML093W2.460.006960.21UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YPL175W2.250.012200.02SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YPR165W2.230.012900.01RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YFR042W2.220.013200.07KEG1Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
YNR011C2.150.015800.05PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YGR198W2.100.018006.27E-4YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YJR141W_p2.100.018100.01YJR141W_pEssential protein of unknown function
YJL081C2.080.018700.05ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YFR037C2.030.021300.00RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR386W15.201.94E-52MUS81Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
YBR098W13.402.40E-41MMS4Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
YBR099C_d10.903.35E-28YBR099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
YGL163C9.786.80E-23RAD54DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
YJL092W8.731.31E-18SRS2DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
YDR004W7.591.62E-14RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YGL175C6.834.40E-12SAE2Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
YDL059C6.513.79E-11RAD59Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
YDR076W6.066.70E-10RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YJL095W5.862.27E-9BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YDR316W5.012.74E-7OMS1Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
YER095W4.691.35E-6RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YAL042W4.542.82E-6ERV46Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport
YLR032W4.337.51E-6RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YCR066W4.308.48E-6RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA

GO enrichment analysis for SGTC_244
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4175.14E-246SGTC_1082camptothecin 424.9 nMTimTec (Natural product library)25380.103448DNA damage response
0.3995.15E-224SGTC_1079idarubicin 2.4 μMNIH Clinical Collection6363620.0736842DNA damage response
0.3971.03E-220SGTC_248streptozotocin 5.2 mMMiscellaneous53000.0405405DNA damage response
0.3432.95E-162SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)31320570.0864198DNA damage response
0.2763.22E-103SGTC_2674-Nitroquinoline-1-oxide (4-NQO) 44.4 nMMiscellaneous59550.236364DNA damage response
0.2748.23E-102SGTC_268camptothecin 39.6 μMMiscellaneous243600.103448DNA damage response
0.2631.17E-93SGTC_1845st056753 3.0 μMTimTec (Natural product derivative library)53758390.174603TSC3-RPN4
0.2081.03E-58SGTC_2671danthron 100.0 μMMicrosource (Natural product library)29500.0701754DNA damage response
0.2005.22E-54SGTC_558k064-0027 21.3 μMChemDiv (Drug-like library)50885260.0694444DNA damage response
0.1967.31E-52SGTC_915methyl methanesulfonate (MMS) 110.0 mMMiscellaneous41560.0408163DNA damage response
0.1932.66E-50SGTC_2677mitomycin C 100.0 μMMiscellaneous57460.0574713DNA damage response
0.1924.93E-50SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.0757576DNA intercalators
0.1905.49E-49SGTC_8291635-0120 117.0 μMChemDiv (Drug-like library)2850540.0625DNA damage response
0.1881.21E-47SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.1DNA damage response
0.1831.29E-45SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.0923077RNA pol III & RNase P/MRP

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_7930488-0545106 μM0.346154292548ChemDiv (Drug-like library)237.212042.24105
SGTC_300r073-00115.04 μM0.32735780ChemDiv (Drug-like library)282.683043.03806
SGTC_524r073-001027.8 μM0.2950822772106ChemDiv (Drug-like library)284.655861.80807
SGTC_12981079-1278263 μM0.2857142840950ChemDiv (Drug-like library)304.258181.65507DNA damage response
SGTC_9123474-00172.18 μM0.281253360550ChemDiv (Drug-like library)312.367983.94307
SGTC_8201582-006059.9 μM0.2786893848840ChemDiv (Drug-like library)290.661983.50816
SGTC_8151582-002736.9 μM0.2727273128206ChemDiv (Drug-like library)286.24292.82717
SGTC_2914130-127835.5 μM0.2714291068044ChemDiv (Drug-like library)375.806223.41505Golgi
SGTC_1832st05411355 μM0.251251267TimTec (Natural product derivative library)364.39783.37116
SGTC_1893970-07422.12 μM0.2456142891807ChemDiv (Drug-like library)297.06172.03104