5628896

N-[(Z)-(2-fluorophenyl)methylideneamino]-1,3-benzothiazol-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2440
Screen concentration 157.8 μM
Source Miscellaneous
PubChem CID 5522048
SMILES C1=CC=C(C(=C1)C=NNC2=NC3=CC=CC=C3S2)F
Standardized SMILES Fc1ccccc1C=NNc2nc3ccccc3s2
Molecular weight 271.3127
ALogP 4.28
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.01
% growth inhibition (Hom. pool) 8.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5522048
Download HIP data (tab-delimited text)  (excel)
Gene:CDC20(YGL116W)|FD-Score:5.62|P-value:9.74E-9|Clearance:0.6||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CUS1(YMR240C)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.06||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:DRE2(YKR071C)|FD-Score:5.01|P-value:2.68E-7|Clearance:0.92||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FOL1(YNL256W)|FD-Score:4.09|P-value:2.16E-5|Clearance:0.08||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:GUS1(YGL245W)|FD-Score:3.13|P-value:8.73E-4|Clearance:0.28||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:HSH49(YOR319W)|FD-Score:7.6|P-value:1.43E-14|Clearance:1.98||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:NUP82(YJL061W)|FD-Score:-4.11|P-value:1.99E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:OLE1(YGL055W)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.18||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PRE8(YML092C)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRP24(YMR268C)|FD-Score:5.63|P-value:9.19E-9|Clearance:0.01||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:SAM50(YNL026W)|FD-Score:3.88|P-value:5.31E-5|Clearance:0.26||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SEC13(YLR208W)|FD-Score:3.6|P-value:1.56E-4|Clearance:0.12||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SGV1(YPR161C)|FD-Score:-3.18|P-value:7.35E-4|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SMD2(YLR275W)|FD-Score:-3.97|P-value:3.53E-5|Clearance:0||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SNU56(YDR240C)|FD-Score:4.01|P-value:2.99E-5|Clearance:0.14||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:TFC3(YAL001C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.02||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRS130(YMR218C)|FD-Score:-3.29|P-value:4.98E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:YGR115C(YGR115C_d)|FD-Score:-3.79|P-value:7.68E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL086C(YJL086C_d)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YKT6(YKL196C)|FD-Score:-3.16|P-value:7.85E-4|Clearance:0||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:CDC20(YGL116W)|FD-Score:5.62|P-value:9.74E-9|Clearance:0.6||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CUS1(YMR240C)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.06||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:DRE2(YKR071C)|FD-Score:5.01|P-value:2.68E-7|Clearance:0.92||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FOL1(YNL256W)|FD-Score:4.09|P-value:2.16E-5|Clearance:0.08||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:GUS1(YGL245W)|FD-Score:3.13|P-value:8.73E-4|Clearance:0.28||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:HSH49(YOR319W)|FD-Score:7.6|P-value:1.43E-14|Clearance:1.98||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:NUP82(YJL061W)|FD-Score:-4.11|P-value:1.99E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:OLE1(YGL055W)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.18||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PRE8(YML092C)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRP24(YMR268C)|FD-Score:5.63|P-value:9.19E-9|Clearance:0.01||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:SAM50(YNL026W)|FD-Score:3.88|P-value:5.31E-5|Clearance:0.26||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SEC13(YLR208W)|FD-Score:3.6|P-value:1.56E-4|Clearance:0.12||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SGV1(YPR161C)|FD-Score:-3.18|P-value:7.35E-4|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SMD2(YLR275W)|FD-Score:-3.97|P-value:3.53E-5|Clearance:0||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SNU56(YDR240C)|FD-Score:4.01|P-value:2.99E-5|Clearance:0.14||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:TFC3(YAL001C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.02||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRS130(YMR218C)|FD-Score:-3.29|P-value:4.98E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:YGR115C(YGR115C_d)|FD-Score:-3.79|P-value:7.68E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL086C(YJL086C_d)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YKT6(YKL196C)|FD-Score:-3.16|P-value:7.85E-4|Clearance:0||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5522048
Download HOP data (tab-delimited text)  (excel)
Gene:ADP1(YCR011C)|FD-Score:4.82|P-value:7.22E-7||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AEP2(YMR282C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM11(YER093C-A)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ASH1(YKL185W)|FD-Score:5.01|P-value:2.72E-7||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:ATG17(YLR423C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:CBF1(YJR060W)|FD-Score:4.45|P-value:4.35E-6||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:COG6(YNL041C)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DUF1(YOL087C)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ENV10(YLR065C)|FD-Score:8.03|P-value:4.71E-16||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:EXG1(YLR300W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FAA2(YER015W)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FAR3(YMR052W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FAR7(YFR008W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCV1(YDR019C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GET3(YDL100C)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:HGH1(YGR187C)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:IRC3(YDR332W)|FD-Score:-5.44|P-value:2.74E-8||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAD3(YJL013C)|FD-Score:-4.52|P-value:3.09E-6||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MTG1(YMR097C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NTO1(YPR031W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PEX31(YGR004W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PKR1(YMR123W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PNS1(YOR161C)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:PTC7(YHR076W)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAS2(YNL098C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RCY1(YJL204C)|FD-Score:5.22|P-value:8.72E-8||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:ROG3(YFR022W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPA14(YDR156W)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPN4(YDL020C)|FD-Score:5.47|P-value:2.21E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTK1(YDL025C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:RTN2(YDL204W)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SEC22(YLR268W)|FD-Score:4.87|P-value:5.62E-7||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHM2(YLR058C)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SLT2(YHR030C)|FD-Score:-3.76|P-value:8.62E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNC1(YAL030W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNX41(YDR425W)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p Gene:SYF2(YGR129W)|FD-Score:6.6|P-value:2.04E-11||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TLG2(YOL018C)|FD-Score:10.1|P-value:2.05E-24||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM70(YNL121C)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:UBC7(YMR022W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VPS52(YDR484W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:8.51|P-value:8.68E-18||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS69(YPR087W_d)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:7.5|P-value:3.23E-14||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAL018C(YAL018C_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function Gene:YCR007C(YCR007C_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDL068W(YDL068W_d)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR157W(YDR157W_d)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR079W(YGR079W_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YGR176W(YGR176W_d)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL115C(YKL115C_d)|FD-Score:7.12|P-value:5.42E-13||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL151C(YKL151C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR279W(YLR279W_d)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR064C(YNR064C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YPK1(YKL126W)|FD-Score:8.04|P-value:4.61E-16||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT52(YKR014C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:ZRC1(YMR243C)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ADP1(YCR011C)|FD-Score:4.82|P-value:7.22E-7||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AEP2(YMR282C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM11(YER093C-A)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ASH1(YKL185W)|FD-Score:5.01|P-value:2.72E-7||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:ATG17(YLR423C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:CBF1(YJR060W)|FD-Score:4.45|P-value:4.35E-6||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:COG6(YNL041C)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DUF1(YOL087C)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ENV10(YLR065C)|FD-Score:8.03|P-value:4.71E-16||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:EXG1(YLR300W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FAA2(YER015W)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FAR3(YMR052W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FAR7(YFR008W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCV1(YDR019C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GET3(YDL100C)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:HGH1(YGR187C)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:IRC3(YDR332W)|FD-Score:-5.44|P-value:2.74E-8||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAD3(YJL013C)|FD-Score:-4.52|P-value:3.09E-6||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MTG1(YMR097C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NTO1(YPR031W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PEX31(YGR004W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PKR1(YMR123W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PNS1(YOR161C)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:PTC7(YHR076W)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAS2(YNL098C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RCY1(YJL204C)|FD-Score:5.22|P-value:8.72E-8||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:ROG3(YFR022W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPA14(YDR156W)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPN4(YDL020C)|FD-Score:5.47|P-value:2.21E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTK1(YDL025C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:RTN2(YDL204W)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SEC22(YLR268W)|FD-Score:4.87|P-value:5.62E-7||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHM2(YLR058C)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SLT2(YHR030C)|FD-Score:-3.76|P-value:8.62E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNC1(YAL030W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNX41(YDR425W)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p Gene:SYF2(YGR129W)|FD-Score:6.6|P-value:2.04E-11||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TLG2(YOL018C)|FD-Score:10.1|P-value:2.05E-24||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM70(YNL121C)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:UBC7(YMR022W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VPS52(YDR484W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:8.51|P-value:8.68E-18||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS69(YPR087W_d)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:7.5|P-value:3.23E-14||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAL018C(YAL018C_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function Gene:YCR007C(YCR007C_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDL068W(YDL068W_d)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR157W(YDR157W_d)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR079W(YGR079W_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YGR176W(YGR176W_d)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL115C(YKL115C_d)|FD-Score:7.12|P-value:5.42E-13||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL151C(YKL151C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR279W(YLR279W_d)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR064C(YNR064C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YPK1(YKL126W)|FD-Score:8.04|P-value:4.61E-16||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT52(YKR014C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:ZRC1(YMR243C)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YOR319W7.601.43E-141.98HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YMR268C5.639.19E-90.01PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YGL116W5.629.74E-90.60CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YKR071C5.012.68E-70.92DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YNL256W4.092.16E-50.08FOL1Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
YDR240C4.012.99E-50.14SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YNL026W3.885.31E-50.26SAM50Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
YAL001C3.621.47E-40.01TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YLR208W3.601.56E-40.12SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YGL055W3.482.47E-40.18OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YJL086C_d3.314.74E-40.11YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YMR240C3.197.00E-40.06CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YGL245W3.138.73E-40.28GUS1Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
YBL092W2.850.002210.00RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YDR238C2.840.002230.05SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL018C10.102.05E-24TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YDR136C_d8.518.68E-18VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YKL126W8.044.61E-16YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR065C8.034.71E-16ENV10Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
YDR372C7.503.23E-14VPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
YKL115C_d7.125.42E-13YKL115C_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
YGR129W6.602.04E-11SYF2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest
YDL020C5.472.21E-8RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YJL204C5.228.72E-8RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YKL185W5.012.72E-7ASH1Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
YLR268W4.875.62E-7SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
YCR011C4.827.22E-7ADP1Putative ATP-dependent permease of the ABC transporter family of proteins
YOR161C4.661.56E-6PNS1Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
YLR279W_d4.621.93E-6YLR279W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL041C4.572.46E-6COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments

GO enrichment analysis for SGTC_2440
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2482.88E-83SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.123077ERAD & cell cycle
0.2231.64E-67SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.232143
0.2174.03E-64SGTC_24435493264 126.7 μMMiscellaneous53400550.6
0.2054.18E-57SGTC_4260988-0037 14.6 μMChemDiv (Drug-like library)53830080.237288ERAD & cell cycle
0.2031.16E-55SGTC_29187977171 22.1 μMChembridge (Drug-like library)22364830.0547945
0.1931.35E-50SGTC_3730844-0104 25.9 μMChemDiv (Drug-like library)53758040.135593ERAD & cell cycle
0.1857.25E-47SGTC_1161k007-0610 110.0 μMChemDiv (Drug-like library)58438500.177419ERAD & cell cycle
0.1823.47E-45SGTC_7334069-0007 56.1 μMChemDiv (Drug-like library)57174250.174603ERAD & cell cycle
0.1783.97E-43SGTC_384k007-0144 5.2 μMChemDiv (Drug-like library)68248860.0972222ERAD & cell cycle
0.1734.11E-41SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.0465116ERAD & cell cycle
0.1681.44E-38SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0545455
0.1628.59E-36SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.1Golgi
0.1497.11E-31SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.0923077fatty acid desaturase (OLE1)
0.1453.47E-29SGTC_813k007-0158 173.0 μMChemDiv (Drug-like library)65239160.145161fatty acid desaturase (OLE1)
0.1411.00E-27SGTC_1030844-0104 51.8 μMChemDiv (Drug-like library)53758040.135593ERAD & cell cycle

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_24315490079200 μM0.7142865722345Miscellaneous267.348824.5614
SGTC_2444548548436.41 μM0.7142866790631Miscellaneous269.321642.39725
SGTC_623k072-010816.9 μM0.6136365382803ChemDiv (Drug-like library)254.31032.92315heme biosynthesis & mitochondrial translocase
SGTC_242556286817.85 μM0.65714958Miscellaneous259.349964.02715
SGTC_243656335228.09 μM0.65724242Miscellaneous254.31033.35215
SGTC_24435493264126.67 μM0.65340055Miscellaneous269.321642.39725
SGTC_2432563086412.22 μM0.5869575714970Miscellaneous243.284363.46915
SGTC_2424553792859.2 μM0.5744685382802Miscellaneous254.31032.92315
SGTC_1883229-0966278.47 μM0.56255722370ChemDiv (Drug-like library)269.321643.83225
SGTC_243954920307.2 μM0.56255722370Miscellaneous269.321643.83225