5162541

(5E)-3-butyl-5-(furan-2-ylmethylidene)-2-sulfanylidene-1,3-thiazolidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2446
Screen concentration 200.0 μM
Source Miscellaneous
PubChem CID 1549321
SMILES CCCCN1C(=O)C(=CC2=CC=CO2)SC1=S
Standardized SMILES CCCCN1C(=S)SC(=Cc2occc2)C1=O
Molecular weight 267.3671
ALogP 3.77
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.8
% growth inhibition (Hom. pool) 6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1549321
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:RPL32(YBL092W)|FD-Score:3.99|P-value:3.29E-5|Clearance:0.43||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN6(YDL097C)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.01||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:4.46|P-value:4.04E-6|Clearance:0.43||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPR2(YIR015W)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPS5(YJR123W)|FD-Score:3.4|P-value:3.35E-4|Clearance:0.17||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RTT105(YER104W)|FD-Score:3.23|P-value:6.22E-4|Clearance:0.05||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC26(YDR238C)|FD-Score:3.15|P-value:8.16E-4|Clearance:0.06||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SPC105(YGL093W)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.16||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SRP14(YDL092W)|FD-Score:3.18|P-value:7.44E-4|Clearance:0.02||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TFG2(YGR005C)|FD-Score:-3.31|P-value:4.62E-4|Clearance:0||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:ULP2(YIL031W)|FD-Score:4.39|P-value:5.62E-6|Clearance:0.43||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:YCS4(YLR272C)|FD-Score:-3.6|P-value:1.62E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YTH1(YPR107C)|FD-Score:4.5|P-value:3.35E-6|Clearance:0.43||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARP7(YPR034W)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:RPL32(YBL092W)|FD-Score:3.99|P-value:3.29E-5|Clearance:0.43||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN6(YDL097C)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.01||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:4.46|P-value:4.04E-6|Clearance:0.43||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPR2(YIR015W)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPS5(YJR123W)|FD-Score:3.4|P-value:3.35E-4|Clearance:0.17||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RTT105(YER104W)|FD-Score:3.23|P-value:6.22E-4|Clearance:0.05||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC26(YDR238C)|FD-Score:3.15|P-value:8.16E-4|Clearance:0.06||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SPC105(YGL093W)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.16||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SRP14(YDL092W)|FD-Score:3.18|P-value:7.44E-4|Clearance:0.02||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TFG2(YGR005C)|FD-Score:-3.31|P-value:4.62E-4|Clearance:0||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:ULP2(YIL031W)|FD-Score:4.39|P-value:5.62E-6|Clearance:0.43||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:YCS4(YLR272C)|FD-Score:-3.6|P-value:1.62E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YTH1(YPR107C)|FD-Score:4.5|P-value:3.35E-6|Clearance:0.43||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1549321
Download HOP data (tab-delimited text)  (excel)
Gene:AIM43(YPL099C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD6(YPL061W)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATG1(YGL180W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATP5(YDR298C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:AVT1(YJR001W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CCS1(YMR038C)|FD-Score:4.32|P-value:7.78E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COX6(YHR051W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CWP1(YKL096W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance Gene:DAL81(YIR023W)|FD-Score:5.38|P-value:3.82E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCC1(YCL016C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:FRD1(YEL047C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HIR1(YBL008W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:IML3(YBR107C)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:LCL3(YGL085W_p)|FD-Score:-3.73|P-value:9.60E-5||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LOC1(YFR001W)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LSM12(YHR121W)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MAD3(YJL013C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MRF1(YGL143C)|FD-Score:4.51|P-value:3.17E-6||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MUP3(YHL036W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NRP1(YDL167C)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NUC1(YJL208C)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:OAR1(YKL055C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PEX30(YLR324W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:POF1(YCL047C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PRM10(YJL108C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:RPS17A(YML024W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCT1(YBL011W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SET4(YJL105W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SLX5(YDL013W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF4(YGL115W)|FD-Score:7.89|P-value:1.48E-15||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:TDA9(YML081W)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP13(YBL067C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UTP30(YKR060W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YBR012C(YBR012C_d)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YDL183C(YDL183C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR286C(YDR286C_p)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YGL041C-B(YGL041C-B_p)|FD-Score:-4.31|P-value:8.26E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL235W(YGL235W_p)|FD-Score:-4.4|P-value:5.51E-6||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YML020W(YML020W_p)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function Gene:YPL062W(YPL062W_d)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR003C(YPR003C_p)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPS3(YLR121C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:AIM43(YPL099C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD6(YPL061W)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATG1(YGL180W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATP5(YDR298C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:AVT1(YJR001W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CCS1(YMR038C)|FD-Score:4.32|P-value:7.78E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COX6(YHR051W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CWP1(YKL096W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance Gene:DAL81(YIR023W)|FD-Score:5.38|P-value:3.82E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCC1(YCL016C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:FRD1(YEL047C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HIR1(YBL008W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:IML3(YBR107C)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:LCL3(YGL085W_p)|FD-Score:-3.73|P-value:9.60E-5||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LOC1(YFR001W)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LSM12(YHR121W)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MAD3(YJL013C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MRF1(YGL143C)|FD-Score:4.51|P-value:3.17E-6||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MUP3(YHL036W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NRP1(YDL167C)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NUC1(YJL208C)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:OAR1(YKL055C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PEX30(YLR324W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:POF1(YCL047C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PRM10(YJL108C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:RPS17A(YML024W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCT1(YBL011W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SET4(YJL105W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SLX5(YDL013W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF4(YGL115W)|FD-Score:7.89|P-value:1.48E-15||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:TDA9(YML081W)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP13(YBL067C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UTP30(YKR060W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YBR012C(YBR012C_d)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YDL183C(YDL183C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR286C(YDR286C_p)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YGL041C-B(YGL041C-B_p)|FD-Score:-4.31|P-value:8.26E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL235W(YGL235W_p)|FD-Score:-4.4|P-value:5.51E-6||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YML020W(YML020W_p)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function Gene:YPL062W(YPL062W_d)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR003C(YPR003C_p)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPS3(YLR121C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C4.503.35E-60.43YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YHR062C4.464.04E-60.43RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YIL031W4.395.62E-60.43ULP2Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate
YBL092W3.993.29E-50.43RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YGL093W3.561.83E-40.16SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YJR123W3.403.35E-40.17RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YER104W3.236.22E-40.05RTT105Protein with a role in regulation of Ty1 transposition
YDL092W3.187.44E-40.02SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
YDL097C3.167.98E-40.01RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YDR238C3.158.16E-40.06SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YCL017C3.090.001010.12NFS1Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
YJL019W2.960.001520.09MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YNL061W2.880.002000.02NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YOR207C2.860.002120.04RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YPL028W2.820.002440.09ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL115W7.891.48E-15SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YIR023W5.383.82E-8DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YPL062W_d4.651.68E-6YPL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
YML081W4.641.76E-6TDA9DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication
YDL183C4.592.24E-6YDL183CMitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
YGL143C4.513.17E-6MRF1Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability
YPL061W4.395.78E-6ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YMR038C4.327.78E-6CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YLR324W4.131.80E-5PEX30Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
YBL067C4.052.58E-5UBP13Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
YBR058C-A3.953.96E-5TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YCL016C3.721.01E-4DCC1Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance
YJL108C3.701.07E-4PRM10Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation
YKR060W3.681.15E-4UTP30Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit
YBL008W3.631.43E-4HIR1Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores

GO enrichment analysis for SGTC_2446
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1629.32E-36SGTC_30641327-0027 10.0 μMChemDiv (Drug-like library)160004550.0769231
0.1541.52E-32SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0882353TSC3-RPN4
0.1533.84E-32SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004550.0769231
0.1529.65E-32SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0895522TSC3-RPN4
0.1475.03E-30SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0588235TSC3-RPN4
0.1397.24E-27SGTC_486niguldipine 82.0 μMMiscellaneous12360.103093amide catabolism
0.1361.04E-25SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.109375RPP1 & pyrimidine depletion
0.1322.41E-24SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0987654TSC3-RPN4
0.1316.24E-24SGTC_24295902844 64.4 μMMiscellaneous19277900.630435
0.1293.12E-23SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.151515
0.1279.58E-23SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.125
0.1279.93E-23SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0617284TSC3-RPN4
0.1271.23E-22SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.12987
0.1263.09E-22SGTC_16014'-hydroxyflavanone 55.5 μMTimTec (Pure natural product library)1655060.106061RPP1 & pyrimidine depletion
0.1241.16E-21SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.107692TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_24625162487171.77 μM0.7727272259986Miscellaneous239.313962.79104
SGTC_2451585622345.7 μM0.7291672179896Miscellaneous269.339942.31105
SGTC_24305571206200 μM0.6938781731771Miscellaneous315.409924.34704
SGTC_24385259590200 μM0.681201127Miscellaneous283.323462.17216
SGTC_2442515890648.48 μM0.6666671893835Miscellaneous251.324663.05904
SGTC_6113453-165223.7 μM0.6346151560575ChemDiv (Drug-like library)283.323462.14906
SGTC_2429590284464.44 μM0.6304351927790Miscellaneous225.287382.44204
SGTC_2452586313648.41 μM0.5918372184096Miscellaneous253.340543.16804
SGTC_24345670830169.91 μM0.4363641549830Miscellaneous250.33992.67404
SGTC_2102537781672.76 μM0.421379702Chembridge (Fragment library)195.19521.33714