5741742

(5E)-3-[(4-bromo-2-methylanilino)methyl]-5-[(4-methoxyphenyl)methylidene]-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2447
Screen concentration 33.8 μM
Source Miscellaneous
PubChem CID 1776504
SMILES CC1=C(C=CC(=C1)Br)NCN2C(=O)C(=CC3=CC=C(C=C3)OC)SC2=O
Standardized SMILES COc1ccc(C=C2SC(=O)N(CNc3ccc(Br)cc3C)C2=O)cc1
Molecular weight 433.3189
ALogP 4.81
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.35
% growth inhibition (Hom. pool) 9.84


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1776504
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRR6(YGL247W)|FD-Score:5.23|P-value:8.35E-8|Clearance:0.16||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CCT6(YDR188W)|FD-Score:-4.19|P-value:1.37E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC20(YGL116W)|FD-Score:-4.81|P-value:7.55E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.74|P-value:9.07E-5|Clearance:0.11||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI13(YLL031C)|FD-Score:3.25|P-value:5.79E-4|Clearance:0.08||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:GPM1(YKL152C)|FD-Score:-3.14|P-value:8.40E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:IRA1(YBR140C)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.04||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LCB1(YMR296C)|FD-Score:3.25|P-value:5.74E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NSE3(YDR288W)|FD-Score:5.36|P-value:4.18E-8|Clearance:0.13||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP82(YJL061W)|FD-Score:5.07|P-value:2.01E-7|Clearance:0.13||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PCF11(YDR228C)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.07||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PMA1(YGL008C)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.46||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RFA2(YNL312W)|FD-Score:4.43|P-value:4.76E-6|Clearance:0.22||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPL30(YGL030W)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPT1(YKL145W)|FD-Score:3.26|P-value:5.48E-4|Clearance:0.01||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SEC13(YLR208W)|FD-Score:6.62|P-value:1.77E-11|Clearance:1.26||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEN34(YAR008W)|FD-Score:3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SPC98(YNL126W)|FD-Score:3.12|P-value:8.91E-4|Clearance:0.1||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SSY1(YDR160W)|FD-Score:4.94|P-value:3.98E-7|Clearance:0.51||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TID3(YIL144W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.15||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:ULP2(YIL031W)|FD-Score:-3.81|P-value:6.84E-5|Clearance:0||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:YCS4(YLR272C)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.16||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:ARP7(YPR034W)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRR6(YGL247W)|FD-Score:5.23|P-value:8.35E-8|Clearance:0.16||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CCT6(YDR188W)|FD-Score:-4.19|P-value:1.37E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC20(YGL116W)|FD-Score:-4.81|P-value:7.55E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.74|P-value:9.07E-5|Clearance:0.11||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI13(YLL031C)|FD-Score:3.25|P-value:5.79E-4|Clearance:0.08||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:GPM1(YKL152C)|FD-Score:-3.14|P-value:8.40E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:IRA1(YBR140C)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.04||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LCB1(YMR296C)|FD-Score:3.25|P-value:5.74E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NSE3(YDR288W)|FD-Score:5.36|P-value:4.18E-8|Clearance:0.13||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP82(YJL061W)|FD-Score:5.07|P-value:2.01E-7|Clearance:0.13||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PCF11(YDR228C)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.07||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PMA1(YGL008C)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.46||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RFA2(YNL312W)|FD-Score:4.43|P-value:4.76E-6|Clearance:0.22||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPL30(YGL030W)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPT1(YKL145W)|FD-Score:3.26|P-value:5.48E-4|Clearance:0.01||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SEC13(YLR208W)|FD-Score:6.62|P-value:1.77E-11|Clearance:1.26||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEN34(YAR008W)|FD-Score:3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SPC98(YNL126W)|FD-Score:3.12|P-value:8.91E-4|Clearance:0.1||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SSY1(YDR160W)|FD-Score:4.94|P-value:3.98E-7|Clearance:0.51||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TID3(YIL144W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.15||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:ULP2(YIL031W)|FD-Score:-3.81|P-value:6.84E-5|Clearance:0||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:YCS4(YLR272C)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.16||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1776504
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:6.55|P-value:2.81E-11||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALF1(YNL148C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARO1(YDR127W)|FD-Score:7.2|P-value:3.02E-13||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASE1(YOR058C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:ATO2(YNR002C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BRP1(YGL007W_d)|FD-Score:4.79|P-value:8.16E-7||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BST1(YFL025C)|FD-Score:3.5|P-value:2.28E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CLN3(YAL040C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CUP9(YPL177C)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-6.32|P-value:1.30E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUN1(YDL101C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:FIG2(YCR089W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FIN1(YDR130C)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FLO11(YIR019C)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:GCN2(YDR283C)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:IRS4(YKR019C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:5.36|P-value:4.10E-8||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:JLP2(YMR132C_p)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:LNP1(YHR192W_p)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MET10(YFR030W)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MSH5(YDL154W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MTF2(YDL044C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NEW1(YPL226W)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NGG1(YDR176W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PAL1(YDR348C)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PET112(YBL080C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX25(YPL112C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Gene:PHA2(YNL316C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PMT2(YAL023C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:RAV1(YJR033C)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REG1(YDR028C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIF2(YLR453C)|FD-Score:5.77|P-value:4.04E-9||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RKM4(YDR257C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RMD8(YFR048W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPL14A(YKL006W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAW1(YAL027W)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SER3(YER081W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SLT2(YHR030C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPO7(YAL009W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SYF2(YGR129W)|FD-Score:5.74|P-value:4.68E-9||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:SYS1(YJL004C)|FD-Score:4.28|P-value:9.35E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:6.06|P-value:6.70E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSR2(YLR435W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBC11(YOR339C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:YAL018C(YAL018C_p)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Putative protein of unknown function Gene:YBL012C(YBL012C_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR064W(YBR064W_d)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR113W(YBR113W_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR137W(YBR137W)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR286C(YDR286C_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YER137C(YER137C_p)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Putative protein of unknown function Gene:YGL039W(YGL039W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YHL045W(YHL045W_d)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function Gene:YIL028W(YIL028W_d)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL193W(YJL193W_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YKL077W(YKL077W_p)|FD-Score:8.3|P-value:5.36E-17||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL147C(YKL147C_d)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YKL169C(YKL169C_d)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR125W(YLR125W_p)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-3.82|P-value:6.72E-5||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL203C(YNL203C_d)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL235C(YNL235C_d)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOR309C(YOR309C_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YOR318C(YOR318C_d)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:ACB1(YGR037C)|FD-Score:6.55|P-value:2.81E-11||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALF1(YNL148C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARO1(YDR127W)|FD-Score:7.2|P-value:3.02E-13||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASE1(YOR058C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:ATO2(YNR002C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BRP1(YGL007W_d)|FD-Score:4.79|P-value:8.16E-7||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BST1(YFL025C)|FD-Score:3.5|P-value:2.28E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CLN3(YAL040C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CUP9(YPL177C)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-6.32|P-value:1.30E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUN1(YDL101C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:FIG2(YCR089W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FIN1(YDR130C)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FLO11(YIR019C)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:GCN2(YDR283C)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:IRS4(YKR019C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:5.36|P-value:4.10E-8||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:JLP2(YMR132C_p)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:LNP1(YHR192W_p)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MET10(YFR030W)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MSH5(YDL154W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MTF2(YDL044C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NEW1(YPL226W)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NGG1(YDR176W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PAL1(YDR348C)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PET112(YBL080C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX25(YPL112C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Gene:PHA2(YNL316C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PMT2(YAL023C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:RAV1(YJR033C)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REG1(YDR028C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIF2(YLR453C)|FD-Score:5.77|P-value:4.04E-9||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RKM4(YDR257C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RMD8(YFR048W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPL14A(YKL006W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAW1(YAL027W)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SER3(YER081W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SLT2(YHR030C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPO7(YAL009W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SYF2(YGR129W)|FD-Score:5.74|P-value:4.68E-9||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:SYS1(YJL004C)|FD-Score:4.28|P-value:9.35E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:6.06|P-value:6.70E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSR2(YLR435W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBC11(YOR339C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:YAL018C(YAL018C_p)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Putative protein of unknown function Gene:YBL012C(YBL012C_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR064W(YBR064W_d)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR113W(YBR113W_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR137W(YBR137W)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR286C(YDR286C_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YER137C(YER137C_p)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Putative protein of unknown function Gene:YGL039W(YGL039W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YHL045W(YHL045W_d)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function Gene:YIL028W(YIL028W_d)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL193W(YJL193W_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YKL077W(YKL077W_p)|FD-Score:8.3|P-value:5.36E-17||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL147C(YKL147C_d)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YKL169C(YKL169C_d)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR125W(YLR125W_p)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-3.82|P-value:6.72E-5||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL203C(YNL203C_d)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL235C(YNL235C_d)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOR309C(YOR309C_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YOR318C(YOR318C_d)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W6.621.77E-111.26SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YDR288W5.364.18E-80.13NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YGL247W5.238.35E-80.17BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YJL061W5.072.01E-70.13NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YDR160W4.943.98E-70.51SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YNL312W4.434.76E-60.22RFA2Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YPR034W4.211.28E-50.01ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YGL008C4.201.34E-50.46PMA1Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
YER133W3.749.07E-50.10GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDR228C3.641.37E-40.07PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YIL144W3.571.79E-40.15TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YLR272C3.423.12E-40.16YCS4Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YKL145W3.265.48E-40.01RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YMR296C3.255.74E-40.00LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YLL031C3.255.79E-40.08GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL077W_p8.305.36E-17YKL077W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YDR127W7.203.02E-13ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YGR037C6.552.81E-11ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YDR007W6.066.70E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YLR453C5.774.04E-9RIF2Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YGR129W5.744.68E-9SYF2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest
YNL265C5.364.10E-8IST1Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p
YER090W4.894.93E-7TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YLR125W_p4.846.39E-7YLR125W_pPutative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene
YGL007W_d4.798.16E-7BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YMR132C_p4.444.46E-6JLP2_pProtein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
YPL090C4.434.64E-6RPS6AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
YDR283C4.356.70E-6GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YJL004C4.289.35E-6SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YAL023C4.112.00E-5PMT2Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2447
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2832.98E-109SGTC_24885268135 45.7 μMMiscellaneous12703560.138889
0.2692.51E-98SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.492308
0.2671.56E-96SGTC_18815652484 16.0 μMMiscellaneous22551290.571429TRP & mitochondrial translation
0.2561.08E-88SGTC_24725377652 49.4 μMMiscellaneous16109030.148649TRP & mitochondrial translation
0.2522.11E-86SGTC_24545-benzylidenerhodanine 10.4 μMMiscellaneous12732100.2TRP & mitochondrial translation
0.2445.03E-81SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.197183
0.2432.81E-80SGTC_18825615643 20.0 μMMiscellaneous22530750.388889TRP & mitochondrial translation
0.2434.04E-80SGTC_7234182-0001 115.0 μMChemDiv (Drug-like library)7448210.150685
0.2404.93E-78SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.2TRP & mitochondrial translation
0.2265.25E-69SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.060241
0.2258.17E-69SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.272727
0.2232.40E-67SGTC_24865283202 22.4 μMMiscellaneous13777510.142857
0.2233.28E-67SGTC_500929-0078 56.4 μMChemDiv (Drug-like library)13777510.142857
0.2215.68E-66SGTC_24455742597 35.6 μMMiscellaneous17742550.807018
0.2201.25E-65SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0921053

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1921574013650 μM0.8113211774243Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_2445574259735.57 μM0.8070181774255Miscellaneous433.318884.80515
SGTC_2441573094639.09 μM0.7627121774275Miscellaneous461.328984.67716
SGTC_1881565248416 μM0.5714292255129Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_24375741531144.16 μM0.5454552262990Miscellaneous402.4392634.24617
SGTC_8191013-025827.7 μM0.4923085753198ChemDiv (Drug-like library)390.90695.13415
SGTC_2450573973712.93 μM0.475412262825Miscellaneous328.3607233.79315
SGTC_2433560787427.82 μM0.4477612252428Miscellaneous374.84134.23515
SGTC_2041543-018462.41 μM0.4411762252213ChemDiv (Drug-like library)356.4138834.76515TRP & mitochondrial translation
SGTC_2453573113222.18 μM0.4285711362040Miscellaneous436.266794.16614