5185112

(5Z)-3-(hydroxymethyl)-5-(thiophen-2-ylmethylidene)-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2448
Screen concentration 27.2 μM
Source Chembridge (Fragment library)
PubChem CID 5718189
SMILES C1=CSC(=C1)C=C2C(=O)N(C(=O)S2)CO
Standardized SMILES OCN1C(=O)SC(=Cc2cccs2)C1=O
Molecular weight 241.2868
ALogP 1.54
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.43
% growth inhibition (Hom. pool) 4.98


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5718189
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:-3.78|P-value:7.76E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB3(YKL088W)|FD-Score:-3.16|P-value:7.91E-4|Clearance:0||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:FRS1(YLR060W)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:MAK11(YKL021C)|FD-Score:3.99|P-value:3.29E-5|Clearance:0.47||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:MAS1(YLR163C)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.11||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NSE3(YDR288W)|FD-Score:6.16|P-value:3.68E-10|Clearance:0.33||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PET9(YBL030C)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.2||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:ROT1(YMR200W)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPC82(YPR190C)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPL30(YGL030W)|FD-Score:7.4|P-value:6.96E-14|Clearance:1.24||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RRN5(YLR141W)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.04||SGD DESC:Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p Gene:RSC3(YDR303C)|FD-Score:4.16|P-value:1.62E-5|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:SEC23(YPR181C)|FD-Score:5.1|P-value:1.65E-7|Clearance:0.02||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC63(YOR254C)|FD-Score:-3.17|P-value:7.70E-4|Clearance:0||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPN1(YPR133C)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.02||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPP2(YOR148C)|FD-Score:5.83|P-value:2.85E-9|Clearance:0.72||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:STN1(YDR082W)|FD-Score:-3.27|P-value:5.31E-4|Clearance:0||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:SUI2(YJR007W)|FD-Score:5.09|P-value:1.82E-7|Clearance:0.93||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TIF34(YMR146C)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.03||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:USB1(YLR132C)|FD-Score:3.28|P-value:5.22E-4|Clearance:0.04||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:ARP7(YPR034W)|FD-Score:-3.78|P-value:7.76E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB3(YKL088W)|FD-Score:-3.16|P-value:7.91E-4|Clearance:0||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:FRS1(YLR060W)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:MAK11(YKL021C)|FD-Score:3.99|P-value:3.29E-5|Clearance:0.47||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:MAS1(YLR163C)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.11||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NSE3(YDR288W)|FD-Score:6.16|P-value:3.68E-10|Clearance:0.33||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PET9(YBL030C)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.2||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:ROT1(YMR200W)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPC82(YPR190C)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPL30(YGL030W)|FD-Score:7.4|P-value:6.96E-14|Clearance:1.24||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RRN5(YLR141W)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.04||SGD DESC:Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p Gene:RSC3(YDR303C)|FD-Score:4.16|P-value:1.62E-5|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:SEC23(YPR181C)|FD-Score:5.1|P-value:1.65E-7|Clearance:0.02||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC63(YOR254C)|FD-Score:-3.17|P-value:7.70E-4|Clearance:0||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPN1(YPR133C)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.02||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPP2(YOR148C)|FD-Score:5.83|P-value:2.85E-9|Clearance:0.72||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:STN1(YDR082W)|FD-Score:-3.27|P-value:5.31E-4|Clearance:0||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:SUI2(YJR007W)|FD-Score:5.09|P-value:1.82E-7|Clearance:0.93||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TIF34(YMR146C)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.03||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:USB1(YLR132C)|FD-Score:3.28|P-value:5.22E-4|Clearance:0.04||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5718189
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG31(YDR022C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:AXL2(YIL140W)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:BIO3(YNR058W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CCM1(YGR150C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CIN8(YEL061C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COG8(YML071C)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:-3.88|P-value:5.14E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DDC1(YPL194W)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:DGR1(YNL130C-A_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DOG2(YHR043C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DPP1(YDR284C)|FD-Score:5.37|P-value:3.93E-8||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:EMP24(YGL200C)|FD-Score:5.27|P-value:6.81E-8||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:GTR1(YML121W)|FD-Score:-3.98|P-value:3.44E-5||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HMS2(YJR147W)|FD-Score:5.81|P-value:3.15E-9||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HXT10(YFL011W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:ISW1(YBR245C)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:LAS21(YJL062W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LIN1(YHR156C)|FD-Score:-3.76|P-value:8.42E-5||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MBP1(YDL056W)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MNN11(YJL183W)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MOT3(YMR070W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MPC2(YHR162W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRPL4(YLR439W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:NFI1(YOR156C)|FD-Score:-3.97|P-value:3.55E-5||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NIT2(YJL126W)|FD-Score:4.32|P-value:7.94E-6||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NMD2(YHR077C)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NTH2(YBR001C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PCL1(YNL289W)|FD-Score:-4.27|P-value:9.88E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX7(YDR142C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PUF2(YPR042C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:PUF4(YGL014W)|FD-Score:3.72|P-value:9.80E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RAD26(YJR035W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RAX1(YOR301W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RPS18A(YDR450W)|FD-Score:4.33|P-value:7.30E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RRF1(YHR038W)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RXT2(YBR095C)|FD-Score:4.94|P-value:3.88E-7||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SBH2(YER019C-A)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SMI1(YGR229C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF4(YGL115W)|FD-Score:9.59|P-value:4.21E-22||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SOP4(YJL192C)|FD-Score:-5.23|P-value:8.43E-8||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO22(YIL073C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:STE50(YCL032W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SYM1(YLR251W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:UBP13(YBL067C)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:YBR226C(YBR226C_d)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL075W(YCL075W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR194W-A(YDR194W-A_p)|FD-Score:6.3|P-value:1.51E-10||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR122C-A(YGR122C-A_d)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YHL008C(YHL008C_p)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHL012W(YHL012W_p)|FD-Score:4.41|P-value:5.23E-6||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YIL102C(YIL102C_p)|FD-Score:5.17|P-value:1.19E-7||SGD DESC:Putative protein of unknown function Gene:YJL144W(YJL144W)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML020W(YML020W_p)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function Gene:YOR111W(YOR111W_p)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Putative protein of unknown function Gene:YOR325W(YOR325W_d)|FD-Score:-4.64|P-value:1.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPL216W(YPL216W_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPR117W(YPR117W_p)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Putative protein of unknown function Gene:AFT1(YGL071W)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG31(YDR022C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:AXL2(YIL140W)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:BIO3(YNR058W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CCM1(YGR150C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CIN8(YEL061C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COG8(YML071C)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:-3.88|P-value:5.14E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DDC1(YPL194W)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:DGR1(YNL130C-A_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DOG2(YHR043C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DPP1(YDR284C)|FD-Score:5.37|P-value:3.93E-8||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:EMP24(YGL200C)|FD-Score:5.27|P-value:6.81E-8||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:GTR1(YML121W)|FD-Score:-3.98|P-value:3.44E-5||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HMS2(YJR147W)|FD-Score:5.81|P-value:3.15E-9||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HXT10(YFL011W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:ISW1(YBR245C)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:LAS21(YJL062W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LIN1(YHR156C)|FD-Score:-3.76|P-value:8.42E-5||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MBP1(YDL056W)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MNN11(YJL183W)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MOT3(YMR070W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MPC2(YHR162W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRPL4(YLR439W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:NFI1(YOR156C)|FD-Score:-3.97|P-value:3.55E-5||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NIT2(YJL126W)|FD-Score:4.32|P-value:7.94E-6||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NMD2(YHR077C)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NTH2(YBR001C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PCL1(YNL289W)|FD-Score:-4.27|P-value:9.88E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX7(YDR142C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PUF2(YPR042C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:PUF4(YGL014W)|FD-Score:3.72|P-value:9.80E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RAD26(YJR035W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RAX1(YOR301W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RPS18A(YDR450W)|FD-Score:4.33|P-value:7.30E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RRF1(YHR038W)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RXT2(YBR095C)|FD-Score:4.94|P-value:3.88E-7||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SBH2(YER019C-A)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SMI1(YGR229C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF4(YGL115W)|FD-Score:9.59|P-value:4.21E-22||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SOP4(YJL192C)|FD-Score:-5.23|P-value:8.43E-8||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO22(YIL073C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:STE50(YCL032W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SYM1(YLR251W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:UBP13(YBL067C)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:YBR226C(YBR226C_d)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL075W(YCL075W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR194W-A(YDR194W-A_p)|FD-Score:6.3|P-value:1.51E-10||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR122C-A(YGR122C-A_d)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YHL008C(YHL008C_p)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHL012W(YHL012W_p)|FD-Score:4.41|P-value:5.23E-6||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YIL102C(YIL102C_p)|FD-Score:5.17|P-value:1.19E-7||SGD DESC:Putative protein of unknown function Gene:YJL144W(YJL144W)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML020W(YML020W_p)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function Gene:YOR111W(YOR111W_p)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Putative protein of unknown function Gene:YOR325W(YOR325W_d)|FD-Score:-4.64|P-value:1.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPL216W(YPL216W_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPR117W(YPR117W_p)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL030W7.406.96E-141.24RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YDR288W6.163.68E-100.33NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YOR148C5.832.85E-90.72SPP2Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
YPR181C5.101.65E-70.02SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YJR007W5.091.82E-70.93SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YDR303C4.161.62E-50.17RSC3Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p
YKL021C3.993.29E-50.47MAK11Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
YLR141W3.522.15E-40.04RRN5Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p
YBL030C3.482.51E-40.20PET9Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
YLR132C3.285.22E-40.04USB1Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
YPR133C3.245.93E-40.02SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YLR163C3.226.39E-40.11MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YMR146C3.119.26E-40.03TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YDR240C3.080.001030.02SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YHR197W3.060.001090.04RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL115W9.594.21E-22SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YDR194W-A_p6.301.51E-10YDR194W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YJR147W5.813.15E-9HMS2Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication
YDR284C5.373.93E-8DPP1Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
YGL200C5.276.81E-8EMP24Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
YIL102C_p5.171.19E-7YIL102C_pPutative protein of unknown function
YBR095C4.943.88E-7RXT2Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth
YHL012W_p4.415.23E-6YHL012W_pPutative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication
YDR450W4.337.30E-6RPS18AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress
YJL126W4.327.94E-6NIT2Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
YDL056W4.181.44E-5MBP1Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
YHR038W3.983.41E-5RRF1Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria
YDR022C3.953.90E-5ATG31Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion
YBR001C3.768.56E-5NTH2Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication
YGL014W3.729.80E-5PUF4Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors

GO enrichment analysis for SGTC_2448
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0732.03E-8SGTC_24465162541 200.0 μMMiscellaneous15493210.288136
0.0732.12E-8SGTC_31139123498 49.5 μMChembridge (Drug-like library)88972750.101449
0.0732.18E-8SGTC_24425158906 48.5 μMMiscellaneous18938350.298246
0.0681.62E-7SGTC_20945332512 191.7 μMChembridge (Fragment library)7557740.1
0.0656.63E-7SGTC_14003771-8998 51.5 μMChemDiv (Drug-like library)6707670.0821918
0.0588.51E-6SGTC_11891431-2119 7.3 μMChemDiv (Drug-like library)31072750.0666667
0.0552.76E-5SGTC_9491165-0505 146.0 μMChemDiv (Drug-like library)62442670.0952381
0.0543.36E-5SGTC_120rhodamine 6G 52.3 μMChemDiv (Drug-like library)652110.0666667mitochondrial processes
0.0534.45E-5SGTC_28939048488 26.0 μMChembridge (Drug-like library)64673380.104478fatty acid desaturase (OLE1)
0.0519.10E-5SGTC_1032k061-1018 98.7 μMChemDiv (Drug-like library)40053930.0833333
0.0501.26E-4SGTC_13201313-0481 95.3 μMChemDiv (Drug-like library)68133650.125
0.0491.43E-4SGTC_3411486-1430 230.0 μMChemDiv (Drug-like library)20547530.0714286
0.0491.57E-4SGTC_20054024620 188.0 μMChembridge (Fragment library)2367430.0862069
0.0491.74E-4SGTC_1064honokiol 6.8 μMNIH Clinical Collection723030.1
0.0491.78E-4SGTC_9651291-5240 308.0 μMChemDiv (Drug-like library)28345980.0769231

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2032518511225.99 μM15718189Chembridge (Fragment library)241.286781.54415
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.4565221241132ChemDiv (Drug-like library)227.32642.79414
SGTC_2241677262537.81 μM0.3921572285411Chembridge (Fragment library)259.280463.09814
SGTC_21365328288199.3 μM0.377778262395Chembridge (Fragment library)222.220540.81724
SGTC_1434032-119415.74 μM0.370371806963ChemDiv (Drug-like library)329.3454833.69515
SGTC_2450573973712.93 μM0.3636362262825Miscellaneous328.3607233.79315
SGTC_2453573113222.18 μM0.3636361362040Miscellaneous436.266794.16614
SGTC_2065522159634.26 μM0.361702788581Chembridge (Fragment library)238.286141.71724
SGTC_1922557818725 μM0.3389831350412Miscellaneous362.8057834.45715TRP & mitochondrial translation
SGTC_2433560787427.82 μM0.3225812252428Miscellaneous374.84134.23515