5812982

1-(5-chloro-2-hydroxyphenyl)-3-(3-fluorophenyl)propane-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2449
Screen concentration 45.8 μM
Source Miscellaneous
PubChem CID 776185
SMILES C1=CC(=CC(=C1)F)C(=O)CC(=O)C2=C(C=CC(=C2)Cl)O
Standardized SMILES Oc1ccc(Cl)cc1C(=O)CC(=O)c2cccc(F)c2
Molecular weight 292.6895
ALogP 3.78
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 27.43
% growth inhibition (Hom. pool) -4.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 776185
Download HIP data (tab-delimited text)  (excel)
Gene:DIM1(YPL266W)|FD-Score:5.89|P-value:1.92E-9|Clearance:1.04||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:DML1(YMR211W)|FD-Score:5.43|P-value:2.78E-8|Clearance:1.04||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FAS1(YKL182W)|FD-Score:3.16|P-value:7.99E-4|Clearance:0.03||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:MET30(YIL046W)|FD-Score:3.42|P-value:3.15E-4|Clearance:0.13||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MTR4(YJL050W)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.08||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NEO1(YIL048W)|FD-Score:3.77|P-value:8.29E-5|Clearance:0.05||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NSE3(YDR288W)|FD-Score:-3.68|P-value:1.17E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PKC1(YBL105C)|FD-Score:3.29|P-value:5.03E-4|Clearance:0.13||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIM2(YBR192W)|FD-Score:4.39|P-value:5.69E-6|Clearance:0.24||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:SDH3(YKL141W)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.26||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SMC1(YFL008W)|FD-Score:3.89|P-value:5.03E-5|Clearance:0.12||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TAF5(YBR198C)|FD-Score:-3.76|P-value:8.54E-5|Clearance:0||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:YCL041C(YCL041C_d)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.3||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:DIM1(YPL266W)|FD-Score:5.89|P-value:1.92E-9|Clearance:1.04||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:DML1(YMR211W)|FD-Score:5.43|P-value:2.78E-8|Clearance:1.04||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FAS1(YKL182W)|FD-Score:3.16|P-value:7.99E-4|Clearance:0.03||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:MET30(YIL046W)|FD-Score:3.42|P-value:3.15E-4|Clearance:0.13||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MTR4(YJL050W)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.08||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NEO1(YIL048W)|FD-Score:3.77|P-value:8.29E-5|Clearance:0.05||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NSE3(YDR288W)|FD-Score:-3.68|P-value:1.17E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PKC1(YBL105C)|FD-Score:3.29|P-value:5.03E-4|Clearance:0.13||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIM2(YBR192W)|FD-Score:4.39|P-value:5.69E-6|Clearance:0.24||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:SDH3(YKL141W)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.26||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SMC1(YFL008W)|FD-Score:3.89|P-value:5.03E-5|Clearance:0.12||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TAF5(YBR198C)|FD-Score:-3.76|P-value:8.54E-5|Clearance:0||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:YCL041C(YCL041C_d)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.3||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 776185
Download HOP data (tab-delimited text)  (excel)
Gene:ADE6(YGR061C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AEP1(YMR064W)|FD-Score:-4.23|P-value:1.15E-5||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AHC2(YCR082W)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM10(YER087W)|FD-Score:-4.41|P-value:5.18E-6||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AVO2(YMR068W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BSC5(YNR069C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CHS7(YHR142W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COX6(YHR051W)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CRS5(YOR031W)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DID2(YKR035W-A)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:DOC1(YGL240W)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:ECM10(YEL030W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:EDS1(YBR033W_p)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EFM2(YBR271W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:FAA2(YER015W)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FIG2(YCR089W)|FD-Score:-3.95|P-value:3.89E-5||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FZO1(YBR179C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GMC1(YDR506C)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GOR1(YNL274C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:HMI1(YOL095C)|FD-Score:-4.12|P-value:1.93E-5||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IRC3(YDR332W)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:ISM1(YPL040C)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:ISW1(YBR245C)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:LCL1(YPL056C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:MBA1(YBR185C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MEF1(YLR069C)|FD-Score:-4.57|P-value:2.43E-6||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MEF2(YJL102W)|FD-Score:-5.24|P-value:8.05E-8||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRPL24(YMR193W)|FD-Score:-6.2|P-value:2.82E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL28(YDR462W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:-6.52|P-value:3.55E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSW1(YDR268W)|FD-Score:-4.69|P-value:1.38E-6||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NAM2(YLR382C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NCS6(YGL211W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NMD2(YHR077C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:ORM2(YLR350W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PEP7(YDR323C)|FD-Score:-3.88|P-value:5.15E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET54(YGR222W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX30(YLR324W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PIF1(YML061C)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:QCR7(YDR529C)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RAS1(YOR101W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RBK1(YCR036W)|FD-Score:5.38|P-value:3.66E-8||SGD DESC:Putative ribokinase Gene:RMD8(YFR048W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Cytosolic protein required for sporulation Gene:RNP1(YLL046C)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RSM27(YGR215W)|FD-Score:-3.12|P-value:9.20E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC6(YPL183W-A)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SLS1(YLR139C)|FD-Score:-4.85|P-value:6.20E-7||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SPT4(YGR063C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUR1(YPL057C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:TRM3(YDL112W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TSC3(YBR058C-A)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TYW3(YGL050W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UPC2(YDR213W)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:VMA4(YOR332W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VMA5(YKL080W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS68(YOL129W)|FD-Score:3.76|P-value:8.63E-5||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YCL042W(YCL042W_p)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR001W(YCR001W_d)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR007C(YCR007C_p)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL114W(YDL114W_p)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.54|P-value:1.96E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR114C(YDR114C_p)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YHL008C(YHL008C_p)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJL007C(YJL007C_d)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL169C(YKL169C_d)|FD-Score:-4.38|P-value:5.94E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YML083C(YML083C_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:-4.66|P-value:1.59E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPK1(YKL126W)|FD-Score:5.29|P-value:6.20E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPK3(YBR028C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:ADE6(YGR061C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AEP1(YMR064W)|FD-Score:-4.23|P-value:1.15E-5||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AHC2(YCR082W)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM10(YER087W)|FD-Score:-4.41|P-value:5.18E-6||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AVO2(YMR068W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BSC5(YNR069C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CHS7(YHR142W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COX6(YHR051W)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CRS5(YOR031W)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DID2(YKR035W-A)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:DOC1(YGL240W)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:ECM10(YEL030W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:EDS1(YBR033W_p)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EFM2(YBR271W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:FAA2(YER015W)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FIG2(YCR089W)|FD-Score:-3.95|P-value:3.89E-5||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FZO1(YBR179C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GMC1(YDR506C)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GOR1(YNL274C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:HMI1(YOL095C)|FD-Score:-4.12|P-value:1.93E-5||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IRC3(YDR332W)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:ISM1(YPL040C)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:ISW1(YBR245C)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:LCL1(YPL056C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:MBA1(YBR185C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MEF1(YLR069C)|FD-Score:-4.57|P-value:2.43E-6||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MEF2(YJL102W)|FD-Score:-5.24|P-value:8.05E-8||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRPL24(YMR193W)|FD-Score:-6.2|P-value:2.82E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL28(YDR462W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:-6.52|P-value:3.55E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSW1(YDR268W)|FD-Score:-4.69|P-value:1.38E-6||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NAM2(YLR382C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NCS6(YGL211W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NMD2(YHR077C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:ORM2(YLR350W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PEP7(YDR323C)|FD-Score:-3.88|P-value:5.15E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET54(YGR222W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX30(YLR324W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PIF1(YML061C)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:QCR7(YDR529C)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RAS1(YOR101W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RBK1(YCR036W)|FD-Score:5.38|P-value:3.66E-8||SGD DESC:Putative ribokinase Gene:RMD8(YFR048W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Cytosolic protein required for sporulation Gene:RNP1(YLL046C)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RSM27(YGR215W)|FD-Score:-3.12|P-value:9.20E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC6(YPL183W-A)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SLS1(YLR139C)|FD-Score:-4.85|P-value:6.20E-7||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SPT4(YGR063C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUR1(YPL057C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:TRM3(YDL112W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TSC3(YBR058C-A)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TYW3(YGL050W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UPC2(YDR213W)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:VMA4(YOR332W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VMA5(YKL080W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS68(YOL129W)|FD-Score:3.76|P-value:8.63E-5||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YCL042W(YCL042W_p)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR001W(YCR001W_d)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR007C(YCR007C_p)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL114W(YDL114W_p)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.54|P-value:1.96E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR114C(YDR114C_p)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YHL008C(YHL008C_p)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJL007C(YJL007C_d)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL169C(YKL169C_d)|FD-Score:-4.38|P-value:5.94E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YML083C(YML083C_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:-4.66|P-value:1.59E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPK1(YKL126W)|FD-Score:5.29|P-value:6.20E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPK3(YBR028C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL266W5.891.92E-91.04DIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
YMR211W5.432.78E-81.04DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YBR192W4.395.69E-60.24RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YKL141W4.151.68E-50.26SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
YFL008W3.895.03E-50.12SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YIL048W3.778.29E-50.05NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YCL041C_d3.711.02E-40.30YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YIL046W3.423.15E-40.13MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YBL105C3.295.03E-40.13PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YKL182W3.167.99E-40.03FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YJL050W3.138.85E-40.08MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YDL008W3.050.001150.03APC11Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
YMR113W3.020.001250.01FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YFR004W3.020.001280.04RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YFR037C2.980.001460.19RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR036W5.383.66E-8RBK1Putative ribokinase
YKL126W5.296.20E-8YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YFR048W4.631.81E-6RMD8Cytosolic protein required for sporulation
YDR213W4.523.16E-6UPC2Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication
YOR332W4.231.15E-5VMA4Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
YPL057C4.201.32E-5SUR1Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
YKR035W-A4.191.37E-5DID2Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
YDL114W_p4.082.29E-5YDL114W_pPutative short-chain dehydrogenase/reductase; YDL114W is not an essential gene
YER015W3.856.02E-5FAA2Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome
YOL129W3.768.63E-5VPS68Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria
YLR324W3.701.08E-4PEX30Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
YHR142W3.621.49E-4CHS7Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
YLR350W3.581.70E-4ORM2Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress
YDL112W3.551.92E-4TRM32'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
YDR003W-A_p3.541.96E-4YDR003W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry

GO enrichment analysis for SGTC_2449
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1554.24E-33SGTC_24595811157 9.2 μMMiscellaneous28706840.707317mitochondrial processes
0.1532.56E-32SGTC_7301436-0052 41.2 μMChemDiv (Drug-like library)7687640.48mitochondrial processes
0.1497.77E-31SGTC_28969052656 71.4 μMChembridge (Drug-like library)64694360.205882endomembrane recycling
0.1479.72E-30SGTC_24755812728 15.0 μMMiscellaneous7761600.72093mitochondrial processes
0.1431.72E-28SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)31320570.0909091DNA damage response
0.1381.78E-26SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.320755
0.1381.68E-26SGTC_2777climbazole 1.5 μMMiscellaneous379070.151515iron homeostasis
0.1381.83E-26SGTC_266formosanin C 406.0 nMMiscellaneous735970.0338983sphingolipid biosynthesis & PDR1
0.1315.41E-24SGTC_24715466956 24.8 μMMiscellaneous7244190.522727mitochondrial processes
0.1318.20E-24SGTC_3393091-4833 71.4 μMChemDiv (Drug-like library)32400770.196721iron homeostasis
0.1241.02E-21SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0762712NEO1
0.1134.60E-18SGTC_1787fluconazole 80.0 μMMiscellaneous33650.16129iron homeostasis
0.1086.60E-17SGTC_2261,3-diallylurea 8.3 mMMiscellaneous745370.0816327
0.1063.98E-16SGTC_431k089-0097 107.0 μMChemDiv (Drug-like library)54994200.185185iron homeostasis
0.1041.42E-15SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.174603NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2475581272815.03 μM0.72093776160Miscellaneous272.2710233.60414mitochondrial processes
SGTC_245958111579.22 μM0.7073172870684Miscellaneous258.2444433.11814mitochondrial processes
SGTC_2471546695624.79 μM0.522727724419Miscellaneous274.699043.57713mitochondrial processes
SGTC_7301436-005241.2 μM0.48768764ChemDiv (Drug-like library)288.2704233.10115mitochondrial processes
SGTC_2477596768625.23 μM0.469388776118Miscellaneous270.279962.89614
SGTC_2481547894845.16 μM0.37254977792Miscellaneous270.279962.89614PDR1
SGTC_24825981709142.65 μM0.339623877755Miscellaneous333.176624.14713PDR1
SGTC_2942905277762.25 μM0.3396236469503Chembridge (Drug-like library)284.1131234.14912
SGTC_3650121-005213.6 μM0.3392863101739ChemDiv (Drug-like library)361.564684.2624mitochondrial response to ROS
SGTC_384k007-01445.16 μM0.3333336824886ChemDiv (Drug-like library)303.743561.91234ERAD & cell cycle