5739737

(5E)-5-benzylidene-3-[(2-fluoroanilino)methyl]-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2450
Screen concentration 12.9 μM
Source Miscellaneous
PubChem CID 2262825
SMILES C1=CC=C(C=C1)C=C2C(=O)N(C(=O)S2)CNC3=CC=CC=C3F
Standardized SMILES Fc1ccccc1NCN2C(=O)SC(=Cc3ccccc3)C2=O
Molecular weight 328.3607
ALogP 3.79
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.85
% growth inhibition (Hom. pool) 4.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2262825
Download HIP data (tab-delimited text)  (excel)
Gene:COP1(YDL145C)|FD-Score:3.8|P-value:7.27E-5|Clearance:0.08||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:CUS1(YMR240C)|FD-Score:3.72|P-value:9.89E-5|Clearance:0.17||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:EXO70(YJL085W)|FD-Score:-7.36|P-value:9.45E-14|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MET30(YIL046W)|FD-Score:-4.43|P-value:4.81E-6|Clearance:0||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NOP19(YGR251W)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:NUP159(YIL115C)|FD-Score:4.7|P-value:1.30E-6|Clearance:0.5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:POL1(YNL102W)|FD-Score:3.14|P-value:8.50E-4|Clearance:0.12||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:PRP11(YDL043C)|FD-Score:3.91|P-value:4.71E-5|Clearance:0.11||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RPB11(YOL005C)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.01||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPC25(YKL144C)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.3||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RRN6(YBL014C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.01||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SKP1(YDR328C)|FD-Score:5.54|P-value:1.55E-8|Clearance:0.83||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SSY1(YDR160W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.39||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:YCS4(YLR272C)|FD-Score:-3.93|P-value:4.19E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YLR339C(YLR339C_d)|FD-Score:-3.27|P-value:5.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:COP1(YDL145C)|FD-Score:3.8|P-value:7.27E-5|Clearance:0.08||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:CUS1(YMR240C)|FD-Score:3.72|P-value:9.89E-5|Clearance:0.17||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:EXO70(YJL085W)|FD-Score:-7.36|P-value:9.45E-14|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MET30(YIL046W)|FD-Score:-4.43|P-value:4.81E-6|Clearance:0||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NOP19(YGR251W)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:NUP159(YIL115C)|FD-Score:4.7|P-value:1.30E-6|Clearance:0.5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:POL1(YNL102W)|FD-Score:3.14|P-value:8.50E-4|Clearance:0.12||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:PRP11(YDL043C)|FD-Score:3.91|P-value:4.71E-5|Clearance:0.11||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RPB11(YOL005C)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.01||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPC25(YKL144C)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.3||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RRN6(YBL014C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.01||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SKP1(YDR328C)|FD-Score:5.54|P-value:1.55E-8|Clearance:0.83||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SSY1(YDR160W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.39||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:YCS4(YLR272C)|FD-Score:-3.93|P-value:4.19E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YLR339C(YLR339C_d)|FD-Score:-3.27|P-value:5.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2262825
Download HOP data (tab-delimited text)  (excel)
Gene:AIM4(YBR194W)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:AKR1(YDR264C)|FD-Score:5.7|P-value:6.16E-9||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALK2(YBL009W)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:APL1(YJR005W)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ATG33(YLR356W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:AYR1(YIL124W)|FD-Score:3.77|P-value:8.19E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BZZ1(YHR114W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:COQ4(YDR204W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:CRG1(YHR209W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CSF1(YLR087C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CST26(YBR042C)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DCG1(YIR030C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DEF1(YKL054C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DSF2(YBR007C_p)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ELP2(YGR200C)|FD-Score:-5.14|P-value:1.34E-7||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EMC5(YIL027C)|FD-Score:-3.74|P-value:9.23E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:END3(YNL084C)|FD-Score:4.47|P-value:4.00E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG3(YLR056W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERG4(YGL012W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAR11(YNL127W)|FD-Score:4.27|P-value:9.70E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FEN2(YCR028C)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:GET4(YOR164C)|FD-Score:4.42|P-value:5.00E-6||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GND2(YGR256W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSP2(YOR185C)|FD-Score:4.83|P-value:6.93E-7||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HSP31(YDR533C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HXT4(YHR092C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:ICT1(YLR099C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IME4(YGL192W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:MDL2(YPL270W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MEI4(YER044C-A)|FD-Score:4.84|P-value:6.55E-7||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MET8(YBR213W)|FD-Score:4.32|P-value:7.65E-6||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MRF1(YGL143C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRP49(YKL167C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:NAM7(YMR080C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NDI1(YML120C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:NHP10(YDL002C)|FD-Score:-3.81|P-value:6.88E-5||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NOT5(YPR072W)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OAR1(YKL055C)|FD-Score:-6.63|P-value:1.74E-11||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PCL6(YER059W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PET111(YMR257C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:POL4(YCR014C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:RIF2(YLR453C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:ROT2(YBR229C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPS11A(YDR025W)|FD-Score:-4.4|P-value:5.30E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:6.59|P-value:2.25E-11||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SER3(YER081W)|FD-Score:-3.72|P-value:9.88E-5||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMF3(YLR034C)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SMP1(YBR182C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication Gene:SPH1(YLR313C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SSK1(YLR006C)|FD-Score:-4.31|P-value:8.23E-6||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SVP26(YHR181W)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SYS1(YJL004C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TIP1(YBR067C)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TUF1(YOR187W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UPS3(YDR185C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:URA2(YJL130C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VEL1(YGL258W)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VFA1(YER128W)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VIP1(YLR410W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VMS1(YDR049W)|FD-Score:-5.22|P-value:9.09E-8||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YCL046W(YCL046W_d)|FD-Score:4.44|P-value:4.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.91|P-value:4.56E-5||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YCR095W-A(YCR095W-A_p)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Putative protein of unknown function Gene:YDL041W(YDL041W_d)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR391C(YDR391C_p)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR455C(YDR455C_d)|FD-Score:-4.84|P-value:6.60E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGR273C(YGR273C_p)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHL015W-A(YHL015W-A_p)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative protein of unknown function Gene:YHR049C-A(YHR049C-A_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL055W(YJL055W_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKL096C-B(YKL096C-B_p)|FD-Score:6.71|P-value:9.79E-12||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL118W(YKL118W_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR125W(YLR125W_p)|FD-Score:5.96|P-value:1.29E-9||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR217W(YLR217W_d)|FD-Score:4.31|P-value:8.01E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YLR326W(YLR326W_p)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YML020W(YML020W_p)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function Gene:YMR153C-A(YMR153C-A_d)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YMR316C-A(YMR316C-A_p)|FD-Score:4.38|P-value:6.00E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YPR108W-A(YPR108W-A_p)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:AIM4(YBR194W)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:AKR1(YDR264C)|FD-Score:5.7|P-value:6.16E-9||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALK2(YBL009W)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:APL1(YJR005W)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ATG33(YLR356W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:AYR1(YIL124W)|FD-Score:3.77|P-value:8.19E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BZZ1(YHR114W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:COQ4(YDR204W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:CRG1(YHR209W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CSF1(YLR087C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CST26(YBR042C)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DCG1(YIR030C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DEF1(YKL054C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DSF2(YBR007C_p)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ELP2(YGR200C)|FD-Score:-5.14|P-value:1.34E-7||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EMC5(YIL027C)|FD-Score:-3.74|P-value:9.23E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:END3(YNL084C)|FD-Score:4.47|P-value:4.00E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG3(YLR056W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERG4(YGL012W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAR11(YNL127W)|FD-Score:4.27|P-value:9.70E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FEN2(YCR028C)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:GET4(YOR164C)|FD-Score:4.42|P-value:5.00E-6||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GND2(YGR256W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSP2(YOR185C)|FD-Score:4.83|P-value:6.93E-7||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HSP31(YDR533C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HXT4(YHR092C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:ICT1(YLR099C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IME4(YGL192W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:MDL2(YPL270W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MEI4(YER044C-A)|FD-Score:4.84|P-value:6.55E-7||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MET8(YBR213W)|FD-Score:4.32|P-value:7.65E-6||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MRF1(YGL143C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRP49(YKL167C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:NAM7(YMR080C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NDI1(YML120C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:NHP10(YDL002C)|FD-Score:-3.81|P-value:6.88E-5||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NOT5(YPR072W)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OAR1(YKL055C)|FD-Score:-6.63|P-value:1.74E-11||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PCL6(YER059W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PET111(YMR257C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:POL4(YCR014C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:RIF2(YLR453C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:ROT2(YBR229C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPS11A(YDR025W)|FD-Score:-4.4|P-value:5.30E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:6.59|P-value:2.25E-11||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SER3(YER081W)|FD-Score:-3.72|P-value:9.88E-5||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMF3(YLR034C)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SMP1(YBR182C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication Gene:SPH1(YLR313C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SSK1(YLR006C)|FD-Score:-4.31|P-value:8.23E-6||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SVP26(YHR181W)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SYS1(YJL004C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TIP1(YBR067C)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TUF1(YOR187W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UPS3(YDR185C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:URA2(YJL130C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VEL1(YGL258W)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VFA1(YER128W)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VIP1(YLR410W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VMS1(YDR049W)|FD-Score:-5.22|P-value:9.09E-8||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YCL046W(YCL046W_d)|FD-Score:4.44|P-value:4.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.91|P-value:4.56E-5||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YCR095W-A(YCR095W-A_p)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Putative protein of unknown function Gene:YDL041W(YDL041W_d)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR391C(YDR391C_p)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR455C(YDR455C_d)|FD-Score:-4.84|P-value:6.60E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGR273C(YGR273C_p)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHL015W-A(YHL015W-A_p)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative protein of unknown function Gene:YHR049C-A(YHR049C-A_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL055W(YJL055W_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKL096C-B(YKL096C-B_p)|FD-Score:6.71|P-value:9.79E-12||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL118W(YKL118W_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR125W(YLR125W_p)|FD-Score:5.96|P-value:1.29E-9||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR217W(YLR217W_d)|FD-Score:4.31|P-value:8.01E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YLR326W(YLR326W_p)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YML020W(YML020W_p)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function Gene:YMR153C-A(YMR153C-A_d)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YMR316C-A(YMR316C-A_p)|FD-Score:4.38|P-value:6.00E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YPR108W-A(YPR108W-A_p)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR328C5.541.55E-80.83SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YIL115C4.701.30E-60.50NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YKL144C4.201.31E-50.30RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YDL043C3.914.71E-50.11PRP11Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YDL145C3.807.27E-50.08COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YMR240C3.729.89E-50.17CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YBL014C3.551.94E-40.01RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YDR160W3.542.00E-40.40SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YOL005C3.148.31E-40.01RPB11RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
YNL102W3.148.50E-40.12POL1Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
YCR035C3.010.001290.05RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YDR235W2.960.001530.08PRP42U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
YDL163W_d2.880.001960.00YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
YOR310C2.880.001970.07NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YNL110C2.810.002470.10NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL096C-B_p6.719.79E-12YKL096C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YPL090C6.592.25E-11RPS6AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
YLR125W_p5.961.29E-9YLR125W_pPutative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene
YMR153C-A_d5.822.91E-9YMR153C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53
YDR264C5.706.16E-9AKR1Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication
YGL258W5.141.36E-7VEL1Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C
YER044C-A4.846.55E-7MEI4Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores
YOR185C4.836.93E-7GSP2GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress
YMR264W4.641.70E-6CUE1Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
YER128W4.631.87E-6VFA1Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology
YPR072W4.513.26E-6NOT5Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
YNL084C4.474.00E-6END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YCL046W_d4.444.57E-6YCL046W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
YOR164C4.425.00E-6GET4Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20
YMR316C-A_p4.386.00E-6YMR316C-A_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W

GO enrichment analysis for SGTC_2450
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0594.86E-6SGTC_1575cyproterone acetate 48.0 μMMiscellaneous98800.0454545
0.0561.78E-5SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.16129cell wall signaling
0.0519.96E-5SGTC_11544088-0362 150.0 μMChemDiv (Drug-like library)66183040.04
0.0501.11E-4SGTC_9373122-0005 46.7 μMChemDiv (Drug-like library)7595350.0714286
0.0482.37E-4SGTC_24535731132 22.2 μMMiscellaneous13620400.632653
0.0473.12E-4SGTC_13772944-0135 198.0 μMChemDiv (Drug-like library)18941370.128205calcium & mitochondrial duress
0.0473.34E-4SGTC_29709090938 41.9 μMChembridge (Drug-like library)172171040.0875RNA processing & uracil transport
0.0463.69E-4SGTC_14843473-2379 286.0 μMChemDiv (Drug-like library)6719170.0945946
0.0464.44E-4SGTC_31349127454 49.5 μMChembridge (Drug-like library)242628200.0724638
0.0455.55E-4SGTC_7911348-1292 225.0 μMChemDiv (Drug-like library)54370540.107143
0.0455.74E-4SGTC_30879117406 49.5 μMChembridge (Drug-like library)425460930.146341
0.0456.04E-4SGTC_1901st060216 58.6 μMTimTec (Natural product derivative library)6887920.0769231
0.0447.26E-4SGTC_797k781-0471 270.0 μMChemDiv (Drug-like library)237381290.111111RPP1 & pyrimidine depletion
0.0447.89E-4SGTC_1220236-0016 402.4 μMChemDiv (Drug-like library)68088380.0689655RNA processing & uracil transport
0.0430.00108SGTC_14294049-0205 9.5 μMChemDiv (Drug-like library)227744780.123077

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1922557818725 μM0.6470591350412Miscellaneous362.8057834.45715TRP & mitochondrial translation
SGTC_2453573113222.18 μM0.6326531362040Miscellaneous436.266794.16614
SGTC_2041543-018462.41 μM0.5714292252213ChemDiv (Drug-like library)356.4138834.76515TRP & mitochondrial translation
SGTC_1921574013650 μM0.5087721774243Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_1881565248416 μM0.5084752255129Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_2433560787427.82 μM0.52252428Miscellaneous374.84134.23515
SGTC_2426558204632.21 μM0.4918032251934Miscellaneous386.3968033.64817
SGTC_24375741531144.16 μM0.4838712262990Miscellaneous402.4392634.24617
SGTC_2445574259735.57 μM0.476191774255Miscellaneous433.318884.80515
SGTC_2447574174233.78 μM0.475411776504Miscellaneous433.318884.80515