5808907

2-[1-(4-chlorophenyl)-2,5-dioxopyrrolidin-3-yl]sulfanylbenzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2458
Screen concentration 16.4 μM
Source Miscellaneous
PubChem CID 2731747
SMILES C1C(C(=O)N(C1=O)C2=CC=C(C=C2)Cl)SC3=CC=CC=C3C(=O)O
Standardized SMILES OC(=O)c1ccccc1SC2CC(=O)N(C2=O)c3ccc(Cl)cc3
Molecular weight 361.7995
ALogP 3.36
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.84
% growth inhibition (Hom. pool) 8.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2731747
Download HIP data (tab-delimited text)  (excel)
Gene:ALR1(YOL130W)|FD-Score:3.25|P-value:5.70E-4|Clearance:0.06||SGD DESC:Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions Gene:APC4(YDR118W)|FD-Score:4.34|P-value:7.22E-6|Clearance:0.32||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:EXO70(YJL085W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.01||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GFA1(YKL104C)|FD-Score:-3.99|P-value:3.32E-5|Clearance:0||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:GWT1(YJL091C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.03||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:NET1(YJL076W)|FD-Score:4.09|P-value:2.15E-5|Clearance:0.32||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP56(YLR197W)|FD-Score:3.95|P-value:3.85E-5|Clearance:0.32||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUG1(YER006W)|FD-Score:4.56|P-value:2.56E-6|Clearance:0.32||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:POP1(YNL221C)|FD-Score:-3.1|P-value:9.63E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP4(YBR257W)|FD-Score:-4.68|P-value:1.46E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:QRI1(YDL103C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.16||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RFC2(YJR068W)|FD-Score:3.19|P-value:7.04E-4|Clearance:0.15||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RLI1(YDR091C)|FD-Score:-3.44|P-value:2.89E-4|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPC25(YKL144C)|FD-Score:-3.26|P-value:5.50E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPP1(YHR062C)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSE1(YML049C)|FD-Score:-3.73|P-value:9.48E-5|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEC23(YPR181C)|FD-Score:-4.28|P-value:9.18E-6|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SPC25(YER018C)|FD-Score:-3.61|P-value:1.52E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:YJL009W(YJL009W_d)|FD-Score:3.37|P-value:3.76E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YOR146W(YOR146W_d)|FD-Score:-3.23|P-value:6.19E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL044C(YPL044C_d)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:ALR1(YOL130W)|FD-Score:3.25|P-value:5.70E-4|Clearance:0.06||SGD DESC:Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions Gene:APC4(YDR118W)|FD-Score:4.34|P-value:7.22E-6|Clearance:0.32||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:EXO70(YJL085W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.01||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GFA1(YKL104C)|FD-Score:-3.99|P-value:3.32E-5|Clearance:0||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:GWT1(YJL091C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.03||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:NET1(YJL076W)|FD-Score:4.09|P-value:2.15E-5|Clearance:0.32||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP56(YLR197W)|FD-Score:3.95|P-value:3.85E-5|Clearance:0.32||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUG1(YER006W)|FD-Score:4.56|P-value:2.56E-6|Clearance:0.32||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:POP1(YNL221C)|FD-Score:-3.1|P-value:9.63E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP4(YBR257W)|FD-Score:-4.68|P-value:1.46E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:QRI1(YDL103C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.16||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RFC2(YJR068W)|FD-Score:3.19|P-value:7.04E-4|Clearance:0.15||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RLI1(YDR091C)|FD-Score:-3.44|P-value:2.89E-4|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPC25(YKL144C)|FD-Score:-3.26|P-value:5.50E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPP1(YHR062C)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSE1(YML049C)|FD-Score:-3.73|P-value:9.48E-5|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEC23(YPR181C)|FD-Score:-4.28|P-value:9.18E-6|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SPC25(YER018C)|FD-Score:-3.61|P-value:1.52E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:YJL009W(YJL009W_d)|FD-Score:3.37|P-value:3.76E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YOR146W(YOR146W_d)|FD-Score:-3.23|P-value:6.19E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL044C(YPL044C_d)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2731747
Download HOP data (tab-delimited text)  (excel)
Gene:CCW12(YLR110C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CKA1(YIL035C)|FD-Score:-3.74|P-value:9.11E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:DAL81(YIR023W)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DNF2(YDR093W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DSS4(YPR017C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:FAR1(YJL157C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FKH1(YIL131C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:NAT3(YPR131C)|FD-Score:4|P-value:3.23E-5||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NOP16(YER002W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:PIN4(YBL051C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PTR2(YKR093W)|FD-Score:-4.31|P-value:8.22E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RBG1(YAL036C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RED1(YLR263W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RSC1(YGR056W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAC1(YKL212W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SNT2(YGL131C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWC5(YBR231C)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:7.2|P-value:2.95E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.69|P-value:6.36E-9||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VID28(YIL017C)|FD-Score:-4.5|P-value:3.37E-6||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VMA1(YDL185W)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:YDR109C(YDR109C_p)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative kinase Gene:YDR521W(YDR521W_d)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function Gene:YGR045C(YGR045C_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR032W(YKR032W_d)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKU80(YMR106C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YNL296W(YNL296W_d)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:CCW12(YLR110C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CKA1(YIL035C)|FD-Score:-3.74|P-value:9.11E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:DAL81(YIR023W)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DNF2(YDR093W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DSS4(YPR017C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:FAR1(YJL157C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FKH1(YIL131C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:NAT3(YPR131C)|FD-Score:4|P-value:3.23E-5||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NOP16(YER002W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:PIN4(YBL051C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PTR2(YKR093W)|FD-Score:-4.31|P-value:8.22E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RBG1(YAL036C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RED1(YLR263W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RSC1(YGR056W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAC1(YKL212W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SNT2(YGL131C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWC5(YBR231C)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:7.2|P-value:2.95E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.69|P-value:6.36E-9||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VID28(YIL017C)|FD-Score:-4.5|P-value:3.37E-6||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VMA1(YDL185W)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:YDR109C(YDR109C_p)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative kinase Gene:YDR521W(YDR521W_d)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function Gene:YGR045C(YGR045C_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR032W(YKR032W_d)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKU80(YMR106C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YNL296W(YNL296W_d)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER006W4.562.56E-60.32NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YDR118W4.347.22E-60.32APC4Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
YJL076W4.092.15E-50.32NET1Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
YLR197W3.953.85E-50.32NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YJL091C3.631.42E-40.03GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YJL085W3.601.61E-40.01EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDL103C3.591.67E-40.16QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YPL044C_d3.433.01E-40.06YPL044C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
YJL009W_d3.373.76E-40.00YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YHR062C3.373.78E-40.12RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOL130W3.255.70E-40.06ALR1Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
YJR068W3.197.04E-40.15RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YPR142C_d3.040.001190.08YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YDL007W2.960.001550.06RPT2One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
YGR009C2.900.001870.03SEC9t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A7.202.95E-13TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML124C5.696.36E-9TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YIR023W4.542.82E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR521W_d4.042.68E-5YDR521W_dDubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
YPR131C4.003.23E-5NAT3Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
YER090W3.924.45E-5TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YBR231C3.807.27E-5SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YGR045C_d3.721.01E-4YGR045C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR093W3.691.14E-4DNF2Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
YER002W3.591.62E-4NOP16Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YGL188C-A_p3.571.76E-4YGL188C-A_pPutative protein of unknown function
YJL157C3.334.36E-4FAR1Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress
YGL131C3.314.61E-4SNT2DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
YJR049C3.304.81E-4UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YDR109C_p3.265.54E-4YDR109C_pPutative kinase

GO enrichment analysis for SGTC_2458
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2593.39E-91SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.056338TSC3-RPN4
0.2596.39E-91SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.151515TSC3-RPN4
0.2453.78E-81SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0571429TSC3-RPN4
0.2353.31E-75SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.126582
0.2343.71E-74SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.151515TSC3-RPN4
0.2192.65E-65SGTC_24615755302 26.1 μMMiscellaneous27889040.791667
0.2173.95E-64SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.166667TSC3-RPN4
0.2158.10E-63SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.213333TSC3-RPN4
0.2101.15E-59SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0909091TSC3-RPN4
0.2082.11E-58SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.119403
0.1982.61E-53SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0571429TSC3-RPN4
0.1978.13E-53SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.119048TSC3-RPN4
0.1951.32E-51SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0869565TSC3-RPN4
0.1951.82E-51SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0933333TSC3-RPN4
0.1904.82E-49SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0930233TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2479561735343.85 μM0.8222222731746Miscellaneous327.354422.69715
SGTC_2461575530226.15 μM0.7916672788904Miscellaneous406.250483.44615
SGTC_2469565501990.81 μM0.7916672788239Miscellaneous341.3813.18315
SGTC_2465580924233.21 μM0.7450982731748Miscellaneous357.38042.68116
SGTC_247358545938.94 μM0.7450982872951Miscellaneous410.486083.77716
SGTC_2470566481721.68 μM0.722859561Miscellaneous361.799483.36215
SGTC_2456577035974.46 μM0.7169813118787Miscellaneous412.45892.54717
SGTC_2487591837344.05 μM0.7169812876279Miscellaneous385.39052.46517
SGTC_2103551-044556.83 μM0.7037042865180ChemDiv (Drug-like library)371.406983.0316
SGTC_2483572905027.16 μM0.7037042865180Miscellaneous371.406983.0316