cycloheximide

4-[(2R)-2-[(3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]piperidine-2,6-dione

Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis thus blocking translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_246
Screen concentration 667.0 μM
Source ICCB bioactive library
PubChem CID 16760040
SMILES CC1CC(C(=O)C(C1)C(CC2CC(=O)NC(=O)C2)O)C
Standardized SMILES CC1CC(C)C(=O)C(C1)C(O)CC2CC(=O)NC(=O)C2
Molecular weight 281.3474
ALogP 0.93
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.44
% growth inhibition (Hom. pool) 8.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16760040
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARC19(YKL013C)|FD-Score:4.77|P-value:9.37E-7|Clearance:0.71||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP7(YPR034W)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.05||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT7(YJL111W)|FD-Score:-6.05|P-value:7.41E-10|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ERV1(YGR029W)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:PRE4(YFR050C)|FD-Score:3.21|P-value:6.62E-4|Clearance:0.13||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PSF3(YOL146W)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPB10(YOR210W)|FD-Score:5.27|P-value:6.93E-8|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB3(YIL021W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.01||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB8(YOR224C)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:3.73|P-value:9.64E-5|Clearance:0.15||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18A(YOL120C)|FD-Score:3.79|P-value:7.51E-5|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL25(YOL127W)|FD-Score:4.91|P-value:4.50E-7|Clearance:0.15||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL5(YPL131W)|FD-Score:5.33|P-value:4.81E-8|Clearance:0.07||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPO26(YPR187W)|FD-Score:4.06|P-value:2.44E-5|Clearance:0.15||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPO31(YOR116C)|FD-Score:6.54|P-value:2.99E-11|Clearance:0.38||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP0(YLR340W)|FD-Score:11.9|P-value:6.29E-33|Clearance:5.35||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPS15(YOL040C)|FD-Score:3.24|P-value:5.98E-4|Clearance:0.03||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP4(YHR069C)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC31(YDL195W)|FD-Score:3.91|P-value:4.60E-5|Clearance:0.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TAH18(YPR048W)|FD-Score:3.85|P-value:5.98E-5|Clearance:0.06||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TIF35(YDR429C)|FD-Score:-3.43|P-value:2.98E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TUB1(YML085C)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.16||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YBR190W(YBR190W_d)|FD-Score:5.19|P-value:1.04E-7|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YLR339C(YLR339C_d)|FD-Score:6.17|P-value:3.47E-10|Clearance:0.83||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:ACT1(YFL039C)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARC19(YKL013C)|FD-Score:4.77|P-value:9.37E-7|Clearance:0.71||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP7(YPR034W)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.05||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT7(YJL111W)|FD-Score:-6.05|P-value:7.41E-10|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ERV1(YGR029W)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:PRE4(YFR050C)|FD-Score:3.21|P-value:6.62E-4|Clearance:0.13||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PSF3(YOL146W)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPB10(YOR210W)|FD-Score:5.27|P-value:6.93E-8|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB3(YIL021W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.01||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB8(YOR224C)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:3.73|P-value:9.64E-5|Clearance:0.15||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18A(YOL120C)|FD-Score:3.79|P-value:7.51E-5|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL25(YOL127W)|FD-Score:4.91|P-value:4.50E-7|Clearance:0.15||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL5(YPL131W)|FD-Score:5.33|P-value:4.81E-8|Clearance:0.07||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPO26(YPR187W)|FD-Score:4.06|P-value:2.44E-5|Clearance:0.15||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPO31(YOR116C)|FD-Score:6.54|P-value:2.99E-11|Clearance:0.38||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP0(YLR340W)|FD-Score:11.9|P-value:6.29E-33|Clearance:5.35||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPS15(YOL040C)|FD-Score:3.24|P-value:5.98E-4|Clearance:0.03||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP4(YHR069C)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC31(YDL195W)|FD-Score:3.91|P-value:4.60E-5|Clearance:0.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TAH18(YPR048W)|FD-Score:3.85|P-value:5.98E-5|Clearance:0.06||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TIF35(YDR429C)|FD-Score:-3.43|P-value:2.98E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TUB1(YML085C)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.16||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YBR190W(YBR190W_d)|FD-Score:5.19|P-value:1.04E-7|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YLR339C(YLR339C_d)|FD-Score:6.17|P-value:3.47E-10|Clearance:0.83||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16760040
Download HOP data (tab-delimited text)  (excel)
Gene:BBC1(YJL020C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BCK1(YJL095W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUD30(YDL151C_d)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:COG8(YML071C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EFT2(YDR385W)|FD-Score:5.92|P-value:1.60E-9||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:END3(YNL084C)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG2(YMR202W)|FD-Score:7.76|P-value:4.22E-15||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:9.42|P-value:2.24E-21||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FYV5(YCL058C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:HPM1(YIL110W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IRC21(YMR073C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JEM1(YJL073W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:JLP2(YMR132C_p)|FD-Score:5.45|P-value:2.45E-8||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:MTD1(YKR080W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NOT5(YPR072W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OMS1(YDR316W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:POP2(YNR052C)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PUG1(YER185W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RPL14A(YKL006W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:4.36|P-value:6.56E-6||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPL6B(YLR448W)|FD-Score:5.06|P-value:2.05E-7||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1A(YDL081C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS24B(YIL069C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:SHU2(YDR078C)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SKI3(YPR189W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLH1(YGR271W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SRC1(YML034W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSD1(YDR293C)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TRM12(YML005W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRS85(YDR108W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP6(YFR010W)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VMS1(YDR049W)|FD-Score:9.21|P-value:1.63E-20||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YDL242W(YDL242W_d)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL214W(YGL214W_d)|FD-Score:5.65|P-value:7.85E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YKL069W(YKL069W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YLR184W(YLR184W_d)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YPQ2(YDR352W_p)|FD-Score:4.54|P-value:2.80E-6||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter Gene:YPR123C(YPR123C_d)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR146C(YPR146C_d)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZUO1(YGR285C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:BBC1(YJL020C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BCK1(YJL095W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUD30(YDL151C_d)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:COG8(YML071C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EFT2(YDR385W)|FD-Score:5.92|P-value:1.60E-9||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:END3(YNL084C)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG2(YMR202W)|FD-Score:7.76|P-value:4.22E-15||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:9.42|P-value:2.24E-21||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FYV5(YCL058C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:HPM1(YIL110W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IRC21(YMR073C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JEM1(YJL073W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:JLP2(YMR132C_p)|FD-Score:5.45|P-value:2.45E-8||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:MTD1(YKR080W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NOT5(YPR072W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OMS1(YDR316W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:POP2(YNR052C)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PUG1(YER185W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RPL14A(YKL006W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:4.36|P-value:6.56E-6||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPL6B(YLR448W)|FD-Score:5.06|P-value:2.05E-7||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1A(YDL081C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS24B(YIL069C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:SHU2(YDR078C)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SKI3(YPR189W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLH1(YGR271W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SRC1(YML034W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSD1(YDR293C)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TRM12(YML005W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRS85(YDR108W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP6(YFR010W)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VMS1(YDR049W)|FD-Score:9.21|P-value:1.63E-20||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YDL242W(YDL242W_d)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL214W(YGL214W_d)|FD-Score:5.65|P-value:7.85E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YKL069W(YKL069W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YLR184W(YLR184W_d)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YPQ2(YDR352W_p)|FD-Score:4.54|P-value:2.80E-6||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter Gene:YPR123C(YPR123C_d)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR146C(YPR146C_d)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZUO1(YGR285C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR340W11.906.29E-335.35RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YOR116C6.542.99E-110.38RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YLR339C_d6.173.47E-100.83YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
YPL131W5.334.81E-80.07RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YOR210W5.276.93E-80.07RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YBR190W_d5.191.04E-70.28YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YOL127W4.914.50E-70.15RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YKL013C4.779.37E-70.70ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YPR187W4.062.44E-50.15RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YDL195W3.914.60E-50.06SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPR048W3.855.98E-50.06TAH18Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance
YOL120C3.797.51E-50.06RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YKL180W3.739.64E-50.15RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YIL021W3.571.75E-40.01RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YML085C3.571.81E-40.16TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL012W9.422.24E-21ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YDR049W9.211.63E-20VMS1Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
YMR202W7.764.22E-15ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YDR385W5.921.60E-9EFT2Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
YGL214W_d5.657.85E-9YGL214W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C
YMR132C_p5.452.45E-8JLP2_pProtein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
YLR448W5.062.05E-7RPL6BRibosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication
YDR352W_p4.542.80E-6YPQ2_pPutative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter
YML073C4.366.56E-6RPL6ARibosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
YFR010W4.356.75E-6UBP6Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance
YDL020C4.356.86E-6RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YDR078C4.072.32E-5SHU2Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
YDR333C_p4.013.10E-5RQC1_pComponent of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC
YPR189W3.934.18E-5SKI3Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
YNL084C3.836.48E-5END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p

GO enrichment analysis for SGTC_246
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1479.16E-30SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.038961ERG2
0.1462.75E-29SGTC_23227413319 38.2 μMChembridge (Fragment library)18404000.0967742ERAD & cell cycle
0.1455.41E-29SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.0307692ERG2
0.1441.05E-28SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0588235ERG2
0.1432.74E-28SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0533333ERG2
0.1433.38E-28SGTC_20995377090 177.4 μMChembridge (Fragment library)22438290.0806452
0.1427.56E-28SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0898876cell wall signaling
0.1331.66E-24SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0806452ERG2
0.1301.18E-23SGTC_2699st077704 54.6 μMTimTec (Natural product derivative library)6458520.0722892endomembrane recycling
0.1294.03E-23SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.0649351ERG2
0.1285.96E-23SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.027027ERG2
0.1255.87E-22SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.0384615ERG2
0.1227.65E-21SGTC_6420335-0876 158.0 μMChemDiv (Drug-like library)68123500.0833333ubiquinone biosynthesis & proteasome
0.1202.54E-20SGTC_488nitrendipine 139.0 μMMiscellaneous45070.0933333
0.1187.92E-20SGTC_1812st052057 4.3 μMTimTec (Natural product derivative library)36454180.1125calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2405hr heat shock (37°C) + Streptovitacin300 μM0.62222291467Miscellaneous297.34682-0.35135heat shock/prefoldin
SGTC_421streptovitacin30 mM0.62222291467Miscellaneous297.34682-0.35135
SGTC_10064099-6547255 μM0.1803283146833ChemDiv (Drug-like library)287.3039063.94313
SGTC_15034585-001261.3 μM0.1780822922052ChemDiv (Drug-like library)338.464943.81214
SGTC_5554296-068537 μM0.1714292908540ChemDiv (Drug-like library)292.419643.11714ergosterol biosynthesis
SGTC_1646st01170975.6 μM0.1694925757843TimTec (Natural product derivative library)233.306162.50112
SGTC_2714st07931356.9 μM0.1692312939799TimTec (Natural product derivative library)351.319753.46916
SGTC_2641levomentholum100 μM0.16666716666Microsource (Natural product library)156.26522.77911
SGTC_2007401153971.82 μM0.15517245595301Chembridge (Fragment library)285.180181.42712cell wall signaling
SGTC_2639lactacystin53 μM0.1527783870Miscellaneous376.42526-0.88758