5473429

2-(4-chlorophenyl)-N-[(Z)-pyridin-4-ylmethylideneamino]acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2463
Screen concentration 200.0 μM
Source Miscellaneous
PubChem CID 5399405
SMILES C1=CC(=CC=C1CC(=O)NN=CC2=CC=NC=C2)Cl
Standardized SMILES Clc1ccc(CC(=O)NN=Cc2ccncc2)cc1
Molecular weight 273.7176
ALogP 2.23
H-bond donor count 1
H-bond acceptor count 3
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.35
% growth inhibition (Hom. pool) 3.25


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5399405
Download HIP data (tab-delimited text)  (excel)
Gene:CDC27(YBL084C)|FD-Score:-4.42|P-value:4.94E-6|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:HIP1(YGR191W)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:HRR25(YPL204W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.15||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:JIP5(YPR169W)|FD-Score:-3.17|P-value:7.53E-4|Clearance:0||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:NSP1(YJL041W)|FD-Score:3.11|P-value:9.20E-4|Clearance:0.18||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:NUP49(YGL172W)|FD-Score:3.85|P-value:5.83E-5|Clearance:0.08||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:RRN11(YML043C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.21||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP4(YHR069C)|FD-Score:5.7|P-value:6.09E-9|Clearance:1.49||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC3(YER008C)|FD-Score:3.74|P-value:9.14E-5|Clearance:0.14||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SLD2(YKL108W)|FD-Score:4.21|P-value:1.31E-5|Clearance:0.07||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SRP72(YPL210C)|FD-Score:-3.39|P-value:3.55E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:UTP6(YDR449C)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YNL150W(YNL150W_d)|FD-Score:4.13|P-value:1.81E-5|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:ZIM17(YNL310C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.13||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix Gene:CDC27(YBL084C)|FD-Score:-4.42|P-value:4.94E-6|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:HIP1(YGR191W)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:HRR25(YPL204W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.15||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:JIP5(YPR169W)|FD-Score:-3.17|P-value:7.53E-4|Clearance:0||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:NSP1(YJL041W)|FD-Score:3.11|P-value:9.20E-4|Clearance:0.18||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:NUP49(YGL172W)|FD-Score:3.85|P-value:5.83E-5|Clearance:0.08||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:RRN11(YML043C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.21||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP4(YHR069C)|FD-Score:5.7|P-value:6.09E-9|Clearance:1.49||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC3(YER008C)|FD-Score:3.74|P-value:9.14E-5|Clearance:0.14||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SLD2(YKL108W)|FD-Score:4.21|P-value:1.31E-5|Clearance:0.07||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SRP72(YPL210C)|FD-Score:-3.39|P-value:3.55E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:UTP6(YDR449C)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YNL150W(YNL150W_d)|FD-Score:4.13|P-value:1.81E-5|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:ZIM17(YNL310C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.13||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5399405
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:-4.77|P-value:9.34E-7||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE12(YNL220W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AGP1(YCL025C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication Gene:APE1(YKL103C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:APN1(YKL114C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG3(YJL088W)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ASM4(YDL088C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:BCK2(YER167W)|FD-Score:3.79|P-value:7.42E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CLD1(YGR110W)|FD-Score:-3.95|P-value:3.89E-5||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COG7(YGL005C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DLT1(YMR126C_p)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:ECM29(YHL030W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:7|P-value:1.31E-12||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GID7(YCL039W)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:HIR3(YJR140C)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HOG1(YLR113W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HTA2(YBL003C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:KTI11(YBL071W-A)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LIP2(YLR239C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MDM36(YPR083W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MLH1(YMR167W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MOH1(YBL049W)|FD-Score:5.56|P-value:1.39E-8||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MPC54(YOR177C)|FD-Score:5.77|P-value:3.98E-9||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRM1(YOR201C)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MTC2(YKL098W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:PUS2(YGL063W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:QCR7(YDR529C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RRT12(YCR045C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RTA1(YGR213C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:RTS1(YOR014W)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAE2(YGL175C)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SMK1(YPR054W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SPA2(YLL021W)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPO22(YIL073C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SPR6(YER115C)|FD-Score:4.27|P-value:9.93E-6||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:TMA19(YKL056C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TPN1(YGL186C)|FD-Score:6.91|P-value:2.50E-12||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRX3(YCR083W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:VAM10(YOR068C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VHS2(YIL135C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:YAR028W(YAR028W_p)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YAR1(YPL239W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YCL023C(YCL023C_d)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCR001W(YCR001W_d)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YDR161W(YDR161W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YER134C(YER134C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YFL019C(YFL019C_d)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGL260W(YGL260W_p)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YHI9(YHR029C)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YJL107C(YJL107C_p)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi Gene:YKR032W(YKR032W_d)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL122C(YNL122C_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR325W(YOR325W_d)|FD-Score:4.83|P-value:6.98E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPC1(YBR183W)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPL216W(YPL216W_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPS1(YLR120C)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:ACB1(YGR037C)|FD-Score:-4.77|P-value:9.34E-7||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE12(YNL220W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AGP1(YCL025C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication Gene:APE1(YKL103C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:APN1(YKL114C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG3(YJL088W)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ASM4(YDL088C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:BCK2(YER167W)|FD-Score:3.79|P-value:7.42E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CLD1(YGR110W)|FD-Score:-3.95|P-value:3.89E-5||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COG7(YGL005C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DLT1(YMR126C_p)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:ECM29(YHL030W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:7|P-value:1.31E-12||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GID7(YCL039W)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:HIR3(YJR140C)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HOG1(YLR113W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HTA2(YBL003C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:KTI11(YBL071W-A)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LIP2(YLR239C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MDM36(YPR083W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MLH1(YMR167W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MOH1(YBL049W)|FD-Score:5.56|P-value:1.39E-8||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MPC54(YOR177C)|FD-Score:5.77|P-value:3.98E-9||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRM1(YOR201C)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MTC2(YKL098W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:PUS2(YGL063W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:QCR7(YDR529C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RRT12(YCR045C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RTA1(YGR213C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:RTS1(YOR014W)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAE2(YGL175C)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SMK1(YPR054W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SPA2(YLL021W)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPO22(YIL073C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SPR6(YER115C)|FD-Score:4.27|P-value:9.93E-6||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:TMA19(YKL056C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TPN1(YGL186C)|FD-Score:6.91|P-value:2.50E-12||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRX3(YCR083W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:VAM10(YOR068C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VHS2(YIL135C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:YAR028W(YAR028W_p)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YAR1(YPL239W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YCL023C(YCL023C_d)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCR001W(YCR001W_d)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YDR161W(YDR161W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YER134C(YER134C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YFL019C(YFL019C_d)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGL260W(YGL260W_p)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YHI9(YHR029C)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YJL107C(YJL107C_p)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi Gene:YKR032W(YKR032W_d)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL122C(YNL122C_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR325W(YOR325W_d)|FD-Score:4.83|P-value:6.98E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPC1(YBR183W)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPL216W(YPL216W_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPS1(YLR120C)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR069C5.706.09E-91.49RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YKL108W4.211.31E-50.07SLD2Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
YNL150W_d4.131.81E-50.28YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YGL172W3.855.83E-50.08NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YDR449C3.787.95E-50.03UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YER008C3.749.14E-50.14SEC3Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
YML043C3.611.55E-40.21RRN11Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YNL310C3.403.39E-40.13ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YPL204W3.275.47E-40.15HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YJL041W3.119.20E-40.18NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YDR355C_d2.930.001670.01YDR355C_dDubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
YDR325W2.920.001750.01YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YDL153C2.910.001780.16SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YLR009W2.750.002960.09RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YJR064W2.670.003850.03CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W7.001.31E-12ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YGL186C6.912.50E-12TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YOR177C5.773.98E-9MPC54Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate
YGL175C5.677.13E-9SAE2Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
YBL049W5.561.39E-8MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YOR325W_d4.836.98E-7YOR325W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1
YPR054W4.661.57E-6SMK1Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p
YOR014W4.651.64E-6RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YJR140C4.483.75E-6HIR3Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
YJL088W4.434.67E-6ARG3Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
YER115C4.279.93E-6SPR6Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation
YOR034C-A_p4.042.62E-5YOR034C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YCR045C3.973.55E-5RRT12Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores
YCL025C3.973.59E-5AGP1Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
YCL039W3.963.70E-5GID7Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions

GO enrichment analysis for SGTC_2463
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1433.14E-28SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0793651ERG2
0.1382.06E-26SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0689655ERG2
0.1292.31E-23SGTC_130199-0087 65.0 μMChemDiv (Drug-like library)45898250.285714ERG2
0.1287.37E-23SGTC_7764476-4378 112.0 μMChemDiv (Drug-like library)28920080.0779221ERG2
0.1263.52E-22SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0547945ERG2
0.1226.67E-21SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.08ERG2
0.1202.30E-20SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0576923ERG2
0.1142.44E-18SGTC_2724maprotiline 69.6 μMMiscellaneous40110.0769231ERG2
0.1054.55E-16SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.0793651ERG2
0.0992.80E-14SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.101266ERG2
0.0993.92E-14SGTC_30429091578 49.5 μMChembridge (Drug-like library)252364970.180556ERG2
0.0976.50E-14SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.111111ERG2
0.0961.11E-13SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.104478ERG2
0.0961.82E-13SGTC_2525harmine 47.9 μMMiscellaneous52809530.078125ergosterol biosynthesis
0.0961.92E-13SGTC_2664lyso-paf C-16 73.5 μMMicrosource (Natural product library)67107420.0666667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_24675474540200 μM0.56255413519Miscellaneous276.718222.92313
SGTC_8820843-0131351 μM0.5416675425421ChemDiv (Drug-like library)278.757243.33613
SGTC_9252064-127259.6 μM0.4909096815817ChemDiv (Drug-like library)333.726481.44825
SGTC_24605552655174.22 μM0.4693885401989Miscellaneous259.6912.62513amide catabolism
SGTC_1884563344425 μM0.4615385724239Miscellaneous287.744162.64113redox potentiating
SGTC_2485563344417.78 μM0.4615385724239Miscellaneous287.744162.64113redox potentiating
SGTC_208k072-009812.37 μM0.4166675948901ChemDiv (Drug-like library)231.68092.96213heme biosynthesis & mitochondrial translocase
SGTC_7051124-0212310 μM0.3921579554485ChemDiv (Drug-like library)293.1484.01212
SGTC_1033k072-010221.3 μM0.3571435965931ChemDiv (Drug-like library)261.299642.1061660S ribosome export
SGTC_563tetrahydrosecaminediol614 μM0.333333334274ChemDiv (Drug-like library)624.898248.24734amide catabolism
SGTC_1166k206-0078225 μM0.3333335404595ChemDiv (Drug-like library)325.749083.40214