5809242

2-[1-(4-methoxyphenyl)-2,5-dioxopyrrolidin-3-yl]sulfanylbenzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2465
Screen concentration 33.2 μM
Source Miscellaneous
PubChem CID 2731748
SMILES COC1=CC=C(C=C1)N2C(=O)CC(C2=O)SC3=CC=CC=C3C(=O)O
Standardized SMILES COc1ccc(cc1)N2C(=O)CC(Sc3ccccc3C(=O)O)C2=O
Molecular weight 357.3804
ALogP 2.68
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.78
% growth inhibition (Hom. pool) 6.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2731748
Download HIP data (tab-delimited text)  (excel)
Gene:ALG13(YGL047W)|FD-Score:-3.12|P-value:9.15E-4|Clearance:0||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:BET1(YIL004C)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.04||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CCT6(YDR188W)|FD-Score:3.21|P-value:6.58E-4|Clearance:0.06||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:DBP10(YDL031W)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.22||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:GRS1(YBR121C)|FD-Score:3.12|P-value:8.96E-4|Clearance:0.02||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HIP1(YGR191W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.36||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:PRE10(YOR362C)|FD-Score:3.15|P-value:8.19E-4|Clearance:0.03||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP39(YML046W)|FD-Score:-3.15|P-value:8.09E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:SMC3(YJL074C)|FD-Score:-3.38|P-value:3.59E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:TCP1(YDR212W)|FD-Score:3.79|P-value:7.62E-5|Clearance:0.36||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TOR2(YKL203C)|FD-Score:-3.79|P-value:7.49E-5|Clearance:0||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:YJL032W(YJL032W_d)|FD-Score:-3.13|P-value:8.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:ALG13(YGL047W)|FD-Score:-3.12|P-value:9.15E-4|Clearance:0||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:BET1(YIL004C)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.04||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CCT6(YDR188W)|FD-Score:3.21|P-value:6.58E-4|Clearance:0.06||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:DBP10(YDL031W)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.22||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:GRS1(YBR121C)|FD-Score:3.12|P-value:8.96E-4|Clearance:0.02||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HIP1(YGR191W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.36||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:PRE10(YOR362C)|FD-Score:3.15|P-value:8.19E-4|Clearance:0.03||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP39(YML046W)|FD-Score:-3.15|P-value:8.09E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:SMC3(YJL074C)|FD-Score:-3.38|P-value:3.59E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:TCP1(YDR212W)|FD-Score:3.79|P-value:7.62E-5|Clearance:0.36||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TOR2(YKL203C)|FD-Score:-3.79|P-value:7.49E-5|Clearance:0||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:YJL032W(YJL032W_d)|FD-Score:-3.13|P-value:8.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2731748
Download HOP data (tab-delimited text)  (excel)
Gene:AMS1(YGL156W)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ATP14(YLR295C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:CHA4(YLR098C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain Gene:CPR4(YCR069W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:DBR1(YKL149C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DPH6(YLR143W_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene Gene:EMP47(YFL048C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ESC8(YOL017W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication Gene:HSC82(YMR186W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:ICL1(YER065C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:KDX1(YKL161C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:MDH1(YKL085W)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:NST1(YNL091W)|FD-Score:-4.36|P-value:6.50E-6||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PTR2(YKR093W)|FD-Score:-4.29|P-value:8.76E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:SOL3(YHR163W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TDA6(YPR157W_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TOS2(YGR221C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TSC3(YBR058C-A)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL177W(YGL177W_d)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGP1(YNL160W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR045C(YGR045C_d)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:AMS1(YGL156W)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ATP14(YLR295C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:CHA4(YLR098C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain Gene:CPR4(YCR069W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:DBR1(YKL149C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DPH6(YLR143W_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene Gene:EMP47(YFL048C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ESC8(YOL017W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication Gene:HSC82(YMR186W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:ICL1(YER065C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:KDX1(YKL161C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:MDH1(YKL085W)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:NST1(YNL091W)|FD-Score:-4.36|P-value:6.50E-6||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PTR2(YKR093W)|FD-Score:-4.29|P-value:8.76E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:SOL3(YHR163W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TDA6(YPR157W_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TOS2(YGR221C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TSC3(YBR058C-A)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL177W(YGL177W_d)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGP1(YNL160W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR045C(YGR045C_d)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR212W3.797.62E-50.36TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YGR191W3.611.53E-40.36HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YIL004C3.255.77E-40.04BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YDR188W3.216.58E-40.06CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YOR362C3.158.19E-40.03PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YBR121C3.128.96E-40.02GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YDL031W3.119.47E-40.21DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YKL125W2.890.001920.10RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YNL188W2.790.002610.00KAR1Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p
YDR478W2.790.002620.04SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YDR238C2.750.002940.03SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YJR067C2.730.003200.04YAE1Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YDR394W2.690.003610.03RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YLR033W2.660.003900.04RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YPR182W2.620.004440.08SMX3Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL161C4.221.23E-5KDX1Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
YBR058C-A4.062.49E-5TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YKL085W3.836.30E-5MDH1Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
YLR295C3.711.03E-4ATP14Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress
YLR143W_p3.591.66E-4DPH6_pDiphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene
YMR186W3.591.66E-4HSC82Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication
YML094C-A_d3.551.93E-4YML094C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
YLR098C3.532.05E-4CHA4DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
YPR157W_p3.532.09E-4TDA6_pPutative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele
YGR221C3.383.63E-4TOS2Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p
YDR182W-A_p3.383.64E-4YDR182W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YKL149C3.334.39E-4DBR1RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition
YHR163W3.255.77E-4SOL36-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
YML124C3.206.78E-4TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YNL160W3.187.39E-4YGP1Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p

GO enrichment analysis for SGTC_2465
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1652.32E-37SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0625TSC3-RPN4
0.1591.12E-34SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0547945TSC3-RPN4
0.1557.01E-33SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.181818
0.1531.99E-32SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0740741TSC3-RPN4
0.1497.27E-31SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.189873TSC3-RPN4
0.1477.00E-30SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.144928TSC3-RPN4
0.1423.88E-28SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0933333TSC3-RPN4
0.1424.07E-28SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.135802
0.1411.31E-27SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.171875TSC3-RPN4
0.1411.48E-27SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0821918TSC3-RPN4
0.1366.17E-26SGTC_24585808907 16.4 μMMiscellaneous27317470.745098
0.1351.60E-25SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0985916TSC3-RPN4
0.1345.53E-25SGTC_10420350-0096 82.0 μMChemDiv (Drug-like library)31225150.132353TSC3-RPN4
0.1316.05E-24SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0684932TSC3-RPN4
0.1283.95E-23SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.311475TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2487591837344.05 μM0.8076922876279Miscellaneous385.39052.46517
SGTC_2103551-044556.83 μM0.7924532865180ChemDiv (Drug-like library)371.406983.0316
SGTC_2483572905027.16 μM0.7924532865180Miscellaneous371.406983.0316
SGTC_2469565501990.81 μM0.782788239Miscellaneous341.3813.18315
SGTC_2479561735343.85 μM0.7708332731746Miscellaneous327.354422.69715
SGTC_2458580890716.39 μM0.7450982731747Miscellaneous361.799483.36215
SGTC_2461575530226.15 μM0.7450982788904Miscellaneous406.250483.44615
SGTC_247358545938.94 μM0.7037042872951Miscellaneous410.486083.77716
SGTC_2456577035974.46 μM0.6785713118787Miscellaneous412.45892.54717
SGTC_2466598340937.09 μM0.6181822879657Miscellaneous345.3448832.90316