mechlorethamine

1,5-dichloropentan-3-amine

Mechlorethamine, a bifunctional alkylating agent, binds to the N7 position on guanine inducing DNA damage and interstrand cross-links.

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PubChem MeSH terms: Alkylating Agents;Antineoplastic Agents, Alkylating;Chemical Warfare Agents;Irritants



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_247
Screen concentration 29.7 μM
Source Miscellaneous
PubChem CID 22133593
SMILES C(CCl)C(CCCl)N
Standardized SMILES NC(CCCl)CCCl
Molecular weight 156.0535
ALogP 0.84
H-bond donor count 1
H-bond acceptor count 1
Response signature DNA damage response

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.24
% growth inhibition (Hom. pool) 4.98


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 22133593
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:4.44|P-value:4.41E-6|Clearance:0.59||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BET4(YJL031C)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.18||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CNS1(YBR155W)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.26||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:GAA1(YLR088W)|FD-Score:3.26|P-value:5.63E-4|Clearance:0.09||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:NOB1(YOR056C)|FD-Score:-3.78|P-value:7.75E-5|Clearance:0||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.02|P-value:2.89E-5|Clearance:0.59||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPI1(YFR003C)|FD-Score:-3.09|P-value:9.89E-4|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:ARP4(YJL081C)|FD-Score:4.44|P-value:4.41E-6|Clearance:0.59||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BET4(YJL031C)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.18||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CNS1(YBR155W)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.26||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:GAA1(YLR088W)|FD-Score:3.26|P-value:5.63E-4|Clearance:0.09||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:NOB1(YOR056C)|FD-Score:-3.78|P-value:7.75E-5|Clearance:0||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.02|P-value:2.89E-5|Clearance:0.59||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPI1(YFR003C)|FD-Score:-3.09|P-value:9.89E-4|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 22133593
Download HOP data (tab-delimited text)  (excel)
Gene:AHP1(YLR109W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:BIM1(YER016W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:CWH41(YGL027C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:EAF7(YNL136W)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EGD2(YHR193C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:EIS1(YMR031C)|FD-Score:-3.93|P-value:4.32E-5||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV25(YML012W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:ERV46(YAL042W)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FAA3(YIL009W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:HSP150(YJL159W)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:KEL2(YGR238C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:MEP3(YPR138C)|FD-Score:4.87|P-value:5.54E-7||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MMS4(YBR098W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MNT4(YNR059W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:NBA1(YOL070C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:POL32(YJR043C)|FD-Score:5.62|P-value:9.32E-9||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PPT2(YPL148C)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PSO2(YMR137C)|FD-Score:9.48|P-value:1.25E-21||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:PUF2(YPR042C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:PUG1(YER185W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RAD1(YPL022W)|FD-Score:7.16|P-value:3.91E-13||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD10(YML095C)|FD-Score:7.03|P-value:1.07E-12||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAD14(YMR201C)|FD-Score:5.29|P-value:6.03E-8||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD18(YCR066W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD2(YGR258C)|FD-Score:5.82|P-value:2.98E-9||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RAD4(YER162C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAD5(YLR032W)|FD-Score:4.54|P-value:2.84E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:REV3(YPL167C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:REV7(YIL139C)|FD-Score:6.79|P-value:5.75E-12||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:SMF3(YLR034C)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SRS2(YJL092W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SWM2(YNR004W_p)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:SYG1(YIL047C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:UBX4(YMR067C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VPS71(YML041C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAL004W(YAL004W_d)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YEL028W(YEL028W_d)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR151C(YGR151C_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YIP4(YGL198W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL007C(YJL007C_d)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR012C(YLR012C_p)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YPR097W(YPR097W)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:AHP1(YLR109W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:BIM1(YER016W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:CWH41(YGL027C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:EAF7(YNL136W)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EGD2(YHR193C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:EIS1(YMR031C)|FD-Score:-3.93|P-value:4.32E-5||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV25(YML012W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:ERV46(YAL042W)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FAA3(YIL009W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:HSP150(YJL159W)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:KEL2(YGR238C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:MEP3(YPR138C)|FD-Score:4.87|P-value:5.54E-7||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MMS4(YBR098W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MNT4(YNR059W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:NBA1(YOL070C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:POL32(YJR043C)|FD-Score:5.62|P-value:9.32E-9||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PPT2(YPL148C)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PSO2(YMR137C)|FD-Score:9.48|P-value:1.25E-21||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:PUF2(YPR042C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:PUG1(YER185W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RAD1(YPL022W)|FD-Score:7.16|P-value:3.91E-13||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD10(YML095C)|FD-Score:7.03|P-value:1.07E-12||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAD14(YMR201C)|FD-Score:5.29|P-value:6.03E-8||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD18(YCR066W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD2(YGR258C)|FD-Score:5.82|P-value:2.98E-9||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RAD4(YER162C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAD5(YLR032W)|FD-Score:4.54|P-value:2.84E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:REV3(YPL167C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:REV7(YIL139C)|FD-Score:6.79|P-value:5.75E-12||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:SMF3(YLR034C)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SRS2(YJL092W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SWM2(YNR004W_p)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:SYG1(YIL047C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:UBX4(YMR067C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VPS71(YML041C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAL004W(YAL004W_d)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YEL028W(YEL028W_d)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR151C(YGR151C_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YIP4(YGL198W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL007C(YJL007C_d)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR012C(YLR012C_p)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YPR097W(YPR097W)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL081C4.444.41E-60.59ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YMR290W-A_d4.022.89E-50.59YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YJL031C3.432.97E-40.18BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YLR088W3.265.63E-40.09GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YBR155W3.167.83E-40.26CNS1TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
YNL151C2.900.001860.20RPC31RNA polymerase III subunit C31
YIL106W2.700.003500.00MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YML127W2.700.003510.06RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YPR034W2.640.004160.09ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YML049C2.550.005390.01RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
YJR045C2.540.005590.09SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YEL034W2.450.007190.04HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YNL290W2.410.007950.00RFC3Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YMR013C2.410.007990.05SEC59Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
YLR010C2.360.009230.12TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR137C9.481.25E-21PSO2Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
YPL022W7.163.91E-13RAD1Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
YML095C7.031.07E-12RAD10Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
YIL139C6.795.75E-12REV7Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
YGR258C5.822.98E-9RAD2Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
YJR043C5.629.32E-9POL32Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
YMR201C5.296.03E-8RAD14Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
YPR138C4.875.54E-7MEP3Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease
YLR032W4.542.84E-6RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YNR004W_p4.241.10E-5SWM2_pPutative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds
YIL047C4.221.24E-5SYG1Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency
YOL070C4.201.31E-5NBA1Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate
YER185W4.062.45E-5PUG1Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins
YLR109W3.953.85E-5AHP1Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
YNL136W3.846.05E-5EAF7Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A

GO enrichment analysis for SGTC_247
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4222.90E-254SGTC_2674-Nitroquinoline-1-oxide (4-NQO) 44.4 nMMiscellaneous5955NADNA damage response
0.3231.65E-143SGTC_2677mitomycin C 100.0 μMMiscellaneous57460.0327869DNA damage response
0.1578.51E-34SGTC_244nsc-207895 1.3 μMMiscellaneous42640NADNA damage response
0.1262.11E-22SGTC_248streptozotocin 5.2 mMMiscellaneous53000.0212766DNA damage response
0.1032.42E-15SGTC_1079idarubicin 2.4 μMNIH Clinical Collection6363620.0138889DNA damage response
0.0937.18E-13SGTC_14254048-4433 7.3 μMChemDiv (Drug-like library)23734946NAcopper-dependent oxidative stress
0.0938.92E-13SGTC_1936st074407 74.3 μMTimTec (Natural product derivative library)809813NADNA damage response
0.0903.53E-12SGTC_915methyl methanesulfonate (MMS) 110.0 mMMiscellaneous4156NADNA damage response
0.0896.22E-12SGTC_251melphalan 456.5 μMMiscellaneous4606120.184211DNA damage response
0.0822.54E-10SGTC_5504123-0385 25.7 μMChemDiv (Drug-like library)57311230.0172414TSC3-RPN4
0.0772.89E-9SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)3132057NADNA damage response
0.0715.70E-8SGTC_930109-0045 59.2 μMChemDiv (Drug-like library)887330.0263158DNA damage response
0.0664.13E-7SGTC_8291635-0120 117.0 μMChemDiv (Drug-like library)285054NADNA damage response
0.0648.02E-7SGTC_600986-0278 202.0 μMChemDiv (Drug-like library)31105980.02
0.0641.01E-6SGTC_558k064-0027 21.3 μMChemDiv (Drug-like library)50885260.0212766DNA damage response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_251melphalan456.52 μM0.184211460612Miscellaneous305.20022-0.11324DNA damage response
SGTC_474dl-dihydrosphingosine5 μM0.1666673058739ICCB bioactive library301.507765.13433
SGTC_1564D-Arginine115 μM0.14705971070TimTec (Pure natural product library)174.20096-3.83144
SGTC_3061glucosamine200 μM0.13793191431Miscellaneous215.63206-2.69966
SGTC_2017513292760.65 μM0.1351358367Chembridge (Fragment library)188.268842.45121
SGTC_2585alliin100 μM0.11764787310Microsource (Natural product library)177.22144-3.39125
SGTC_438sphingosine6.67 μM0.1111115280335ICCB bioactive library299.491884.82433
SGTC_393acivicin6.25 μM0.10810816758175Miscellaneous178.57368-3.2525
SGTC_451chloral hydrate100 μM0.1052632707Miscellaneous165.403020.89922
SGTC_12290139-019312.2 μM0.13090752ChemDiv (Drug-like library)280.366423.61212RSC complex & mRNA processing