streptozotocin

1-methyl-1-nitroso-3-[2,4,5-trihydroxy-6-(hydroxymethyl)oxan-3-yl]urea

DNA alkylating agent.

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PubChem MeSH terms: Antibiotics, Antineoplastic



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_248
Screen concentration 5.2 mM
Source Miscellaneous
PubChem CID 5300
SMILES CN(C(=O)NC1C(C(C(OC1O)CO)O)O)N=O
Standardized SMILES CN(N=O)C(=O)NC1C(O)OC(CO)C(O)C1O
Molecular weight 265.2206
ALogP -2.29
H-bond donor count 5
H-bond acceptor count 8
Response signature DNA damage response

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.49
% growth inhibition (Hom. pool) 5.97


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5300
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:6.94|P-value:1.94E-12|Clearance:0.71||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARC19(YKL013C)|FD-Score:-3.87|P-value:5.47E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP4(YJL081C)|FD-Score:8.69|P-value:1.89E-18|Clearance:0.48||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:3.92|P-value:4.45E-5|Clearance:0.02||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDS1(YBR029C)|FD-Score:-3.23|P-value:6.27E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC2(YDL209C)|FD-Score:3.3|P-value:4.76E-4|Clearance:0.09||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DFR1(YOR236W)|FD-Score:4.22|P-value:1.24E-5|Clearance:0.04||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FHL1(YPR104C)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.22||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:LSM2(YBL026W)|FD-Score:7.73|P-value:5.54E-15|Clearance:0.76||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NDC1(YML031W)|FD-Score:4.17|P-value:1.49E-5|Clearance:0.26||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NSP1(YJL041W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.07||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PAB1(YER165W)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.29||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:POL30(YBR088C)|FD-Score:5.65|P-value:8.26E-9|Clearance:0.88||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:PRP24(YMR268C)|FD-Score:8.21|P-value:1.12E-16|Clearance:0.06||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PUP2(YGR253C)|FD-Score:5.9|P-value:1.78E-9|Clearance:0.26||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:3.21|P-value:6.59E-4|Clearance:0.04||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RFA1(YAR007C)|FD-Score:3.12|P-value:8.99E-4|Clearance:0.03||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RPB3(YIL021W)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.03||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:6.97|P-value:1.63E-12|Clearance:0.02||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL32(YBL092W)|FD-Score:4.77|P-value:9.42E-7|Clearance:0.44||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPP1(YHR062C)|FD-Score:-4.51|P-value:3.21E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:8.15|P-value:1.85E-16|Clearance:0.42||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:10.5|P-value:2.85E-26|Clearance:1.85||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:6.01|P-value:9.35E-10|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SFH1(YLR321C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SMD1(YGR074W)|FD-Score:-3.31|P-value:4.60E-4|Clearance:0||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SPC105(YGL093W)|FD-Score:4.3|P-value:8.36E-6|Clearance:0.09||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SUP45(YBR143C)|FD-Score:4.33|P-value:7.61E-6|Clearance:0.02||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TIM12(YBR091C)|FD-Score:3.09|P-value:9.92E-4|Clearance:0.08||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:YTH1(YPR107C)|FD-Score:6.23|P-value:2.30E-10|Clearance:0.22||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:6.94|P-value:1.94E-12|Clearance:0.71||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARC19(YKL013C)|FD-Score:-3.87|P-value:5.47E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP4(YJL081C)|FD-Score:8.69|P-value:1.89E-18|Clearance:0.48||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:3.92|P-value:4.45E-5|Clearance:0.02||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDS1(YBR029C)|FD-Score:-3.23|P-value:6.27E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC2(YDL209C)|FD-Score:3.3|P-value:4.76E-4|Clearance:0.09||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DFR1(YOR236W)|FD-Score:4.22|P-value:1.24E-5|Clearance:0.04||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FHL1(YPR104C)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.22||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:LSM2(YBL026W)|FD-Score:7.73|P-value:5.54E-15|Clearance:0.76||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NDC1(YML031W)|FD-Score:4.17|P-value:1.49E-5|Clearance:0.26||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NSP1(YJL041W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.07||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PAB1(YER165W)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.29||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:POL30(YBR088C)|FD-Score:5.65|P-value:8.26E-9|Clearance:0.88||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:PRP24(YMR268C)|FD-Score:8.21|P-value:1.12E-16|Clearance:0.06||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PUP2(YGR253C)|FD-Score:5.9|P-value:1.78E-9|Clearance:0.26||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:3.21|P-value:6.59E-4|Clearance:0.04||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RFA1(YAR007C)|FD-Score:3.12|P-value:8.99E-4|Clearance:0.03||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RPB3(YIL021W)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.03||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:6.97|P-value:1.63E-12|Clearance:0.02||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL32(YBL092W)|FD-Score:4.77|P-value:9.42E-7|Clearance:0.44||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPP1(YHR062C)|FD-Score:-4.51|P-value:3.21E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:8.15|P-value:1.85E-16|Clearance:0.42||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:10.5|P-value:2.85E-26|Clearance:1.85||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:6.01|P-value:9.35E-10|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SFH1(YLR321C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SMD1(YGR074W)|FD-Score:-3.31|P-value:4.60E-4|Clearance:0||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SPC105(YGL093W)|FD-Score:4.3|P-value:8.36E-6|Clearance:0.09||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SUP45(YBR143C)|FD-Score:4.33|P-value:7.61E-6|Clearance:0.02||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TIM12(YBR091C)|FD-Score:3.09|P-value:9.92E-4|Clearance:0.08||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:YTH1(YPR107C)|FD-Score:6.23|P-value:2.30E-10|Clearance:0.22||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5300
Download HOP data (tab-delimited text)  (excel)
Gene:AIM33(YML087C)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:AVT5(YBL089W)|FD-Score:6.88|P-value:2.97E-12||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BTT1(YDR252W)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:CAC2(YML102W)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:COX16(YJL003W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CSM2(YIL132C)|FD-Score:4.81|P-value:7.67E-7||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:CWC21(YDR482C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:ECM7(YLR443W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:ELG1(YOR144C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:ESC2(YDR363W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:IRC4(YDR540C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MAG1(YER142C)|FD-Score:8.44|P-value:1.64E-17||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MCH2(YKL221W)|FD-Score:4.27|P-value:9.56E-6||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MMS2(YGL087C)|FD-Score:7.01|P-value:1.20E-12||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MMS4(YBR098W)|FD-Score:9.39|P-value:2.86E-21||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MPH1(YIR002C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MSD1(YPL104W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MST1(YKL194C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MUS81(YDR386W)|FD-Score:10.8|P-value:1.08E-27||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:PAP2(YOL115W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PEX15(YOL044W)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PEX29(YDR479C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PMS1(YNL082W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:POL32(YJR043C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PSY3(YLR376C)|FD-Score:6.62|P-value:1.84E-11||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RAD5(YLR032W)|FD-Score:8.63|P-value:3.04E-18||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD54(YGL163C)|FD-Score:5.05|P-value:2.26E-7||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:6.38|P-value:8.83E-11||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:6.02|P-value:8.49E-10||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:7.06|P-value:8.48E-13||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RPL20A(YMR242C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:6.44|P-value:5.86E-11||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAE2(YGL175C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SNQ2(YDR011W)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SNZ2(YNL333W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SRS2(YJL092W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SUR4(YLR372W)|FD-Score:-4.31|P-value:8.08E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAL1(YLR354C)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:UBC13(YDR092W)|FD-Score:8.07|P-value:3.57E-16||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:UBP8(YMR223W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:URA7(YBL039C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:YBL029W(YBL029W_p)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Non-essential protein of unknown function Gene:YBR099C(YBR099C_d)|FD-Score:8.77|P-value:8.74E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YDR008C(YDR008C_d)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR442W(YDR442W_d)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL136W(YKL136W_d)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR177W(YLR177W_p)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR352W(YLR352W_p)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene Gene:YPL191C(YPL191C_p)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR1(YDR368W)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:YPS1(YLR120C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:AIM33(YML087C)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:AVT5(YBL089W)|FD-Score:6.88|P-value:2.97E-12||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BTT1(YDR252W)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:CAC2(YML102W)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:COX16(YJL003W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CSM2(YIL132C)|FD-Score:4.81|P-value:7.67E-7||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:CWC21(YDR482C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:ECM7(YLR443W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:ELG1(YOR144C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:ESC2(YDR363W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:IRC4(YDR540C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MAG1(YER142C)|FD-Score:8.44|P-value:1.64E-17||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MCH2(YKL221W)|FD-Score:4.27|P-value:9.56E-6||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MMS2(YGL087C)|FD-Score:7.01|P-value:1.20E-12||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MMS4(YBR098W)|FD-Score:9.39|P-value:2.86E-21||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MPH1(YIR002C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MSD1(YPL104W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MST1(YKL194C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MUS81(YDR386W)|FD-Score:10.8|P-value:1.08E-27||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:PAP2(YOL115W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PEX15(YOL044W)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PEX29(YDR479C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PMS1(YNL082W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:POL32(YJR043C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PSY3(YLR376C)|FD-Score:6.62|P-value:1.84E-11||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RAD5(YLR032W)|FD-Score:8.63|P-value:3.04E-18||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD54(YGL163C)|FD-Score:5.05|P-value:2.26E-7||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:6.38|P-value:8.83E-11||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:6.02|P-value:8.49E-10||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:7.06|P-value:8.48E-13||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RPL20A(YMR242C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:6.44|P-value:5.86E-11||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAE2(YGL175C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SNQ2(YDR011W)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SNZ2(YNL333W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SRS2(YJL092W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SUR4(YLR372W)|FD-Score:-4.31|P-value:8.08E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAL1(YLR354C)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:UBC13(YDR092W)|FD-Score:8.07|P-value:3.57E-16||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:UBP8(YMR223W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:URA7(YBL039C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:YBL029W(YBL029W_p)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Non-essential protein of unknown function Gene:YBR099C(YBR099C_d)|FD-Score:8.77|P-value:8.74E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YDR008C(YDR008C_d)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR442W(YDR442W_d)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL136W(YKL136W_d)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR177W(YLR177W_p)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR352W(YLR352W_p)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene Gene:YPL191C(YPL191C_p)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR1(YDR368W)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:YPS1(YLR120C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFR037C10.502.85E-261.85RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YJL081C8.691.89E-180.48ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YMR268C8.211.12E-160.06PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YLR033W8.151.85E-160.42RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YBL026W7.735.54E-150.76LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YDR404C6.971.63E-120.02RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YNR016C6.941.94E-120.71ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YPR107C6.232.30E-100.22YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YML127W6.019.35E-100.10RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YGR253C5.901.78E-90.26PUP2Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
YBR088C5.658.26E-90.88POL30Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
YBL092W4.779.42E-70.44RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YBR143C4.337.61E-60.02SUP45Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
YGL093W4.308.36E-60.09SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YOR236W4.221.24E-50.04DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR386W10.801.08E-27MUS81Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
YBR098W9.392.86E-21MMS4Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
YBR099C_d8.778.74E-19YBR099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
YLR032W8.633.04E-18RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YER142C8.441.64E-17MAG13-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress
YDR092W8.073.57E-16UBC13Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
YDL059C7.068.48E-13RAD59Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
YGL087C7.011.20E-12MMS2Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
YBL089W6.882.97E-12AVT5Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication
YLR376C6.621.84E-11PSY3Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
YJL047C6.445.86E-11RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YDR076W6.388.83E-11RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YDR004W6.028.49E-10RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YGL163C5.052.26E-7RAD54DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
YJL092W4.983.13E-7SRS2DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability

GO enrichment analysis for SGTC_248
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4032.80E-229SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)31320570.0375DNA damage response
0.3971.03E-220SGTC_244nsc-207895 1.3 μMMiscellaneous426400.0405405DNA damage response
0.3955.72E-220SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.084507RSC complex & mRNA processing
0.3657.82E-186SGTC_268camptothecin 39.6 μMMiscellaneous243600.0581395DNA damage response
0.3486.56E-168SGTC_1079idarubicin 2.4 μMNIH Clinical Collection6363620.114943DNA damage response
0.3471.98E-166SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.101449RSC complex & mRNA processing
0.3402.45E-159SGTC_1082camptothecin 424.9 nMTimTec (Natural product library)25380.0581395DNA damage response
0.3357.82E-155SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.0333333RSC complex & mRNA processing
0.3246.01E-144SGTC_8291635-0120 117.0 μMChemDiv (Drug-like library)285054NADNA damage response
0.3216.04E-141SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0273973RSC complex & mRNA processing
0.3202.80E-140SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0151515RSC complex & mRNA processing
0.3113.78E-132SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.0508475RSC complex & mRNA processing
0.3051.96E-127SGTC_10503232-1156 69.3 μMChemDiv (Drug-like library)7005290.0615385RSC complex & mRNA processing
0.2951.74E-118SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.0689655RSC complex & mRNA processing
0.2922.38E-116SGTC_2674-Nitroquinoline-1-oxide (4-NQO) 44.4 nMMiscellaneous59550.016129DNA damage response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_15346-azauridine81.6 μM0.241379233502TimTec (Pure natural product library)245.18944-2.43247RNA pol III & RNase P/MRP
SGTC_1569carminic acid40.6 μM0.23943714749TimTec (Pure natural product library)492.3864-0.436913
SGTC_2490carminic acid95.39 μM0.23943714749TimTec (Pure natural product library)492.3864-0.436913
SGTC_2615-fluorouridine15.38 μM0.2372881821Miscellaneous262.191843-2.54147exosome
SGTC_1789tunicamycin25 nM0.23076923847177ICCB bioactive library718.70344-4.5341116unfolded protein response
SGTC_255tunicamycin200.47 nM0.23076923847177ICCB bioactive library718.70344-4.5341116unfolded protein response
SGTC_1528arabinosylhypoxanthine74.6 μM0.21875804TimTec (Pure natural product library)268.22608-2.24946
SGTC_1956st07708554 μM0.21428624208685TimTec (Natural product derivative library)370.30812-0.531510
SGTC_1540guanosine70.6 μM0.2121216802TimTec (Pure natural product library)283.24072-2.3856
SGTC_2688kanamycin b96.69 μM0.206349636396Microsource (Natural product library)581.59236-8.041319calcium & mitochondrial duress
SGTC_1539benzyladenosine56 μM0.19718392208TimTec (Pure natural product library)357.363860.25848
SGTC_2410aloe-emodin-8-o-glycoside46.3 μM0.19178114077415TimTec (Pure natural product library)432.3775-0.21610mitochondrial processes