5767542

2-[2,5-dioxo-1-[2-(trifluoromethyl)phenyl]pyrrolidin-3-yl]sulfanylbenzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2480
Screen concentration 37.2 μM
Source Miscellaneous
PubChem CID 2867358
SMILES C1C(C(=O)N(C1=O)C2=CC=CC=C2C(F)(F)F)SC3=CC=CC=C3C(=O)O
Standardized SMILES OC(=O)c1ccccc1SC2CC(=O)N(C2=O)c3ccccc3C(F)(F)F
Molecular weight 395.3524
ALogP 3.64
H-bond donor count 1
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.45
% growth inhibition (Hom. pool) 6.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2867358
Download HIP data (tab-delimited text)  (excel)
Gene:GPN3(YLR243W_p)|FD-Score:5.59|P-value:1.13E-8|Clearance:0.9||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:MDN1(YLR106C)|FD-Score:-3.48|P-value:2.46E-4|Clearance:0||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NOP56(YLR197W)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.1||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NSP1(YJL041W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.22||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:POP6(YGR030C)|FD-Score:5|P-value:2.90E-7|Clearance:0.9||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:POP8(YBL018C)|FD-Score:4.09|P-value:2.12E-5|Clearance:0.31||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RHO3(YIL118W)|FD-Score:-3.88|P-value:5.28E-5|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPP1(YHR062C)|FD-Score:3.78|P-value:7.81E-5|Clearance:0.34||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TEL2(YGR099W)|FD-Score:3.12|P-value:8.96E-4|Clearance:0.07||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TFA1(YKL028W)|FD-Score:-3.09|P-value:9.97E-4|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:UTP6(YDR449C)|FD-Score:-3.35|P-value:4.03E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL152W(YDL152W_d)|FD-Score:5.44|P-value:2.70E-8|Clearance:0.9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:GPN3(YLR243W_p)|FD-Score:5.59|P-value:1.13E-8|Clearance:0.9||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:MDN1(YLR106C)|FD-Score:-3.48|P-value:2.46E-4|Clearance:0||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NOP56(YLR197W)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.1||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NSP1(YJL041W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.22||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:POP6(YGR030C)|FD-Score:5|P-value:2.90E-7|Clearance:0.9||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:POP8(YBL018C)|FD-Score:4.09|P-value:2.12E-5|Clearance:0.31||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RHO3(YIL118W)|FD-Score:-3.88|P-value:5.28E-5|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPP1(YHR062C)|FD-Score:3.78|P-value:7.81E-5|Clearance:0.34||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TEL2(YGR099W)|FD-Score:3.12|P-value:8.96E-4|Clearance:0.07||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TFA1(YKL028W)|FD-Score:-3.09|P-value:9.97E-4|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:UTP6(YDR449C)|FD-Score:-3.35|P-value:4.03E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL152W(YDL152W_d)|FD-Score:5.44|P-value:2.70E-8|Clearance:0.9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2867358
Download HOP data (tab-delimited text)  (excel)
Gene:AEP1(YMR064W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM44(YPL158C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:APA1(YCL050C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:API2(YDR525W_d)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ATG8(YBL078C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BDF2(YDL070W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BYE1(YKL005C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit Gene:CLB3(YDL155W)|FD-Score:4.45|P-value:4.21E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CRP1(YHR146W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTR9(YOL145C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DSD1(YGL196W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:DSE2(YHR143W)|FD-Score:6.71|P-value:9.47E-12||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EDC1(YGL222C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:EEB1(YPL095C)|FD-Score:-4.97|P-value:3.41E-7||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EMC4(YGL231C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERG2(YMR202W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:-3.11|P-value:9.35E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FAA4(YMR246W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FSH2(YMR222C)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GMC1(YDR506C)|FD-Score:-5.12|P-value:1.54E-7||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GPT2(YKR067W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HIS5(YIL116W)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HST3(YOR025W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:IME4(YGL192W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IOC3(YFR013W)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:KAP114(YGL241W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KRE2(YDR483W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LSM6(YDR378C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LYS1(YIR034C)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAL33(YBR297W)|FD-Score:-4.51|P-value:3.29E-6||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MNL2(YLR057W)|FD-Score:-3.89|P-value:5.09E-5||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRPL32(YCR003W)|FD-Score:-5.09|P-value:1.82E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NCE102(YPR149W)|FD-Score:-3.73|P-value:9.65E-5||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:NFU1(YKL040C)|FD-Score:-4.96|P-value:3.61E-7||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:RNQ1(YCL028W)|FD-Score:4.88|P-value:5.24E-7||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPL2A(YFR031C-A)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPL37B(YDR500C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRT7(YLL030C_d)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:STB4(YMR019W)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STF2(YGR008C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:SWM2(YNR004W_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:SYC1(YOR179C)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:SYT1(YPR095C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TAN1(YGL232W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TDA8(YAL064C-A_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TDH2(YJR009C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:6.26|P-value:1.91E-10||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBC11(YOR339C)|FD-Score:-4.92|P-value:4.26E-7||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UIP5(YKR044W)|FD-Score:-4.32|P-value:7.65E-6||SGD DESC:Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates Gene:VPS27(YNR006W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS36(YLR417W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR144C(YBR144C_d)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Gene:YDL187C(YDL187C_d)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR010C(YDR010C_d)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR015C(YDR015C_d)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR426C(YDR426C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YGL082W(YGL082W_p)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGR042W(YGR042W_p)|FD-Score:5.04|P-value:2.33E-7||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YHR022C(YHR022C_p)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YIL001W(YIL001W_p)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIM2(YMR151W_d)|FD-Score:-4.56|P-value:2.59E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YJL007C(YJL007C_d)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL027W(YKL027W)|FD-Score:-4|P-value:3.14E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL031W(YKL031W_d)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YLR050C(YLR050C_p)|FD-Score:-5.46|P-value:2.40E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YME2(YMR302C)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YNL140C(YNL140C_p)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNL195C(YNL195C_p)|FD-Score:5.53|P-value:1.57E-8||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR223W(YOR223W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOS9(YDR057W)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPS7(YDR349C)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:AEP1(YMR064W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM44(YPL158C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:APA1(YCL050C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:API2(YDR525W_d)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ATG8(YBL078C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BDF2(YDL070W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BYE1(YKL005C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit Gene:CLB3(YDL155W)|FD-Score:4.45|P-value:4.21E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CRP1(YHR146W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTR9(YOL145C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DSD1(YGL196W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:DSE2(YHR143W)|FD-Score:6.71|P-value:9.47E-12||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EDC1(YGL222C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:EEB1(YPL095C)|FD-Score:-4.97|P-value:3.41E-7||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EMC4(YGL231C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERG2(YMR202W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:-3.11|P-value:9.35E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FAA4(YMR246W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FSH2(YMR222C)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GMC1(YDR506C)|FD-Score:-5.12|P-value:1.54E-7||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GPT2(YKR067W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HIS5(YIL116W)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HST3(YOR025W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:IME4(YGL192W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IOC3(YFR013W)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:KAP114(YGL241W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KRE2(YDR483W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LSM6(YDR378C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LYS1(YIR034C)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAL33(YBR297W)|FD-Score:-4.51|P-value:3.29E-6||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MNL2(YLR057W)|FD-Score:-3.89|P-value:5.09E-5||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRPL32(YCR003W)|FD-Score:-5.09|P-value:1.82E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NCE102(YPR149W)|FD-Score:-3.73|P-value:9.65E-5||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:NFU1(YKL040C)|FD-Score:-4.96|P-value:3.61E-7||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:RNQ1(YCL028W)|FD-Score:4.88|P-value:5.24E-7||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPL2A(YFR031C-A)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPL37B(YDR500C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRT7(YLL030C_d)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:STB4(YMR019W)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STF2(YGR008C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:SWM2(YNR004W_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:SYC1(YOR179C)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:SYT1(YPR095C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TAN1(YGL232W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TDA8(YAL064C-A_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TDH2(YJR009C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:6.26|P-value:1.91E-10||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBC11(YOR339C)|FD-Score:-4.92|P-value:4.26E-7||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UIP5(YKR044W)|FD-Score:-4.32|P-value:7.65E-6||SGD DESC:Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates Gene:VPS27(YNR006W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS36(YLR417W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR144C(YBR144C_d)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Gene:YDL187C(YDL187C_d)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR010C(YDR010C_d)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR015C(YDR015C_d)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR426C(YDR426C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YGL082W(YGL082W_p)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGR042W(YGR042W_p)|FD-Score:5.04|P-value:2.33E-7||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YHR022C(YHR022C_p)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YIL001W(YIL001W_p)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIM2(YMR151W_d)|FD-Score:-4.56|P-value:2.59E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YJL007C(YJL007C_d)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL027W(YKL027W)|FD-Score:-4|P-value:3.14E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL031W(YKL031W_d)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YLR050C(YLR050C_p)|FD-Score:-5.46|P-value:2.40E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YME2(YMR302C)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YNL140C(YNL140C_p)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNL195C(YNL195C_p)|FD-Score:5.53|P-value:1.57E-8||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR223W(YOR223W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOS9(YDR057W)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPS7(YDR349C)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR243W_p5.591.13E-80.90GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YDL152W_d5.442.70E-80.90YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YGR030C5.002.90E-70.90POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YBL018C4.092.12E-50.31POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR062C3.787.81E-50.34RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR197W3.442.93E-40.10NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YJL041W3.344.20E-40.22NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YGR099W3.128.96E-40.07TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YGL093W3.050.001130.15SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YBR140C2.900.001860.06IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YDR526C_d2.840.002270.02YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YFR031C2.820.002400.04SMC2Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
YMR185W_p2.780.002680.01RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YPL238C_d2.780.002730.00YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YDR324C2.780.002750.03UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR143W6.719.47E-12DSE2Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
YBR058C-A6.261.91E-10TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YNL195C_p5.531.57E-8YNL195C_pPutative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGR042W_p5.042.33E-7YGR042W_pProtein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
YCL028W4.885.24E-7RNQ1[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate
YDL155W4.454.21E-6CLB3B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
YMR222C4.385.88E-6FSH2Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
YMR246W4.231.15E-5FAA4Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication
YBL078C4.221.22E-5ATG8Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
YIL116W4.012.98E-5HIS5Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
YOR179C3.865.58E-5SYC1Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication
YFR013W3.855.99E-5IOC3Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
YJL007C_d3.749.28E-5YJL007C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL196W3.661.27E-4DSD1D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate
YOL145C3.631.44E-4CTR9Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats

GO enrichment analysis for SGTC_2480
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1681.39E-38SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.161765TSC3-RPN4
0.1672.51E-38SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0547945TSC3-RPN4
0.1484.06E-30SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.160494TSC3-RPN4
0.1423.67E-28SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0609756TSC3-RPN4
0.1398.30E-27SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.133333TSC3-RPN4
0.1381.36E-26SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.135802
0.1343.77E-25SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0540541TSC3-RPN4
0.1331.40E-24SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0933333TSC3-RPN4
0.1323.50E-24SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0972222TSC3-RPN4
0.1292.47E-23SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0898876TSC3-RPN4
0.1261.98E-22SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0625TSC3-RPN4
0.1262.98E-22SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.0886076TSC3-RPN4
0.1241.19E-21SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.114286
0.1187.93E-20SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.108108
0.1171.81E-19SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.234568TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2466598340937.09 μM0.7450982879657Miscellaneous345.3448832.90316
SGTC_2470566481721.68 μM0.7115382859561Miscellaneous361.799483.36215
SGTC_2457573157211.68 μM0.6727272865357Miscellaneous355.407583.66915
SGTC_2476580691610.51 μM0.6666672870194Miscellaneous395.352393.63918
SGTC_2479561735343.85 μM0.6666672731746Miscellaneous327.354422.69715
SGTC_2484580981812.04 μM0.6315792870548Miscellaneous355.407583.66915
SGTC_2458580890716.39 μM0.6181822731747Miscellaneous361.799483.36215
SGTC_2461575530226.15 μM0.6181822788904Miscellaneous406.250483.44615
SGTC_2469565501990.81 μM0.6181822788239Miscellaneous341.3813.18315
SGTC_2465580924233.21 μM0.5862072731748Miscellaneous357.38042.68116