5729050

2-[1-(4-ethoxyphenyl)-2,5-dioxopyrrolidin-3-yl]sulfanylbenzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2483
Screen concentration 27.2 μM
Source Miscellaneous
PubChem CID 2865180
SMILES CCOC1=CC=C(C=C1)N2C(=O)CC(C2=O)SC3=CC=CC=C3C(=O)O
Standardized SMILES CCOc1ccc(cc1)N2C(=O)CC(Sc3ccccc3C(=O)O)C2=O
Molecular weight 371.407
ALogP 3.03
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.88
% growth inhibition (Hom. pool) 11.09


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2865180
Download HIP data (tab-delimited text)  (excel)
Gene:ARC35(YNR035C)|FD-Score:-3.24|P-value:5.92E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:EPL1(YFL024C)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.02||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:GPI14(YJR013W)|FD-Score:3.26|P-value:5.67E-4|Clearance:0.19||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HSF1(YGL073W)|FD-Score:3.31|P-value:4.66E-4|Clearance:0.06||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:LCP5(YER127W)|FD-Score:-6.07|P-value:6.43E-10|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MCM3(YEL032W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MCM4(YPR019W)|FD-Score:4.67|P-value:1.50E-6|Clearance:0.09||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:MPS3(YJL019W)|FD-Score:6.26|P-value:1.97E-10|Clearance:1.33||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:MTR4(YJL050W)|FD-Score:6|P-value:9.83E-10|Clearance:1.33||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NET1(YJL076W)|FD-Score:-3.6|P-value:1.57E-4|Clearance:0||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP56(YLR197W)|FD-Score:-4.24|P-value:1.14E-5|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP1(YOR098C)|FD-Score:4.26|P-value:1.00E-5|Clearance:0.2||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:ORC3(YLL004W)|FD-Score:3.77|P-value:8.14E-5|Clearance:0.06||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RET2(YFR051C)|FD-Score:3.91|P-value:4.58E-5|Clearance:0.02||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPC53(YDL150W)|FD-Score:-3.28|P-value:5.12E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:4.35|P-value:6.66E-6|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP9(YPR137W)|FD-Score:3.89|P-value:4.91E-5|Clearance:0.04||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SPC105(YGL093W)|FD-Score:3.7|P-value:1.10E-4|Clearance:0.39||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPC3(YLR066W)|FD-Score:4.58|P-value:2.28E-6|Clearance:0.23||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SUI1(YNL244C)|FD-Score:3.86|P-value:5.75E-5|Clearance:0.09||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TIF35(YDR429C)|FD-Score:-3.21|P-value:6.74E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM22(YDL217C)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.11||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:YJL015C(YJL015C_d)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YPL238C(YPL238C_d)|FD-Score:4.06|P-value:2.43E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ARC35(YNR035C)|FD-Score:-3.24|P-value:5.92E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:EPL1(YFL024C)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.02||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:GPI14(YJR013W)|FD-Score:3.26|P-value:5.67E-4|Clearance:0.19||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HSF1(YGL073W)|FD-Score:3.31|P-value:4.66E-4|Clearance:0.06||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:LCP5(YER127W)|FD-Score:-6.07|P-value:6.43E-10|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MCM3(YEL032W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MCM4(YPR019W)|FD-Score:4.67|P-value:1.50E-6|Clearance:0.09||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:MPS3(YJL019W)|FD-Score:6.26|P-value:1.97E-10|Clearance:1.33||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:MTR4(YJL050W)|FD-Score:6|P-value:9.83E-10|Clearance:1.33||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NET1(YJL076W)|FD-Score:-3.6|P-value:1.57E-4|Clearance:0||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP56(YLR197W)|FD-Score:-4.24|P-value:1.14E-5|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP1(YOR098C)|FD-Score:4.26|P-value:1.00E-5|Clearance:0.2||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:ORC3(YLL004W)|FD-Score:3.77|P-value:8.14E-5|Clearance:0.06||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RET2(YFR051C)|FD-Score:3.91|P-value:4.58E-5|Clearance:0.02||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPC53(YDL150W)|FD-Score:-3.28|P-value:5.12E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:4.35|P-value:6.66E-6|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP9(YPR137W)|FD-Score:3.89|P-value:4.91E-5|Clearance:0.04||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SPC105(YGL093W)|FD-Score:3.7|P-value:1.10E-4|Clearance:0.39||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPC3(YLR066W)|FD-Score:4.58|P-value:2.28E-6|Clearance:0.23||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SUI1(YNL244C)|FD-Score:3.86|P-value:5.75E-5|Clearance:0.09||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TIF35(YDR429C)|FD-Score:-3.21|P-value:6.74E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM22(YDL217C)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.11||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:YJL015C(YJL015C_d)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YPL238C(YPL238C_d)|FD-Score:4.06|P-value:2.43E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2865180
Download HOP data (tab-delimited text)  (excel)
Gene:ADE6(YGR061C)|FD-Score:4.29|P-value:9.02E-6||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARG82(YDR173C)|FD-Score:4.85|P-value:6.29E-7||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG7(YHR171W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BOI1(YBL085W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:12.9|P-value:3.47E-38||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CIN2(YPL241C)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLB6(YGR109C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CTF18(YMR078C)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CUS2(YNL286W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DUR3(YHL016C)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ERT1(YBR239C_p)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:ERV14(YGL054C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ETR1(YBR026C)|FD-Score:-4.72|P-value:1.17E-6||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FDC1(YDR539W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FIR1(YER032W)|FD-Score:-3.99|P-value:3.27E-5||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FLO11(YIR019C)|FD-Score:6.01|P-value:9.26E-10||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FRD1(YEL047C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FRE3(YOR381W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FRE6(YLL051C)|FD-Score:4.81|P-value:7.55E-7||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GIR2(YDR152W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GIS4(YML006C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:GLE2(YER107C)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GUP1(YGL084C)|FD-Score:-6.28|P-value:1.65E-10||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GYP1(YOR070C)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBS1(YKR084C)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HER2(YMR293C)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HIS2(YFR025C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HSP26(YBR072W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HSP31(YDR533C)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HST1(YOL068C)|FD-Score:5.62|P-value:9.39E-9||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IOC3(YFR013W)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC4(YDR540C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:ISU2(YOR226C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KRE6(YPR159W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LTE1(YAL024C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MHO1(YJR008W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MNT3(YIL014W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MOT2(YER068W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:NBP2(YDR162C)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NTG1(YAL015C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:OCT1(YKL134C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OXR1(YPL196W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PBP2(YBR233W)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PCI8(YIL071C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:POL32(YJR043C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:POP2(YNR052C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PTC7(YHR076W)|FD-Score:-4.58|P-value:2.35E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTR2(YKR093W)|FD-Score:-4.57|P-value:2.39E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAD6(YGL058W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RCE1(YMR274C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RIM11(YMR139W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RME1(YGR044C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPA12(YJR063W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL19A(YBR084C-A)|FD-Score:4.31|P-value:8.00E-6||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:4.28|P-value:9.43E-6||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RTT106(YNL206C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAC1(YKL212W)|FD-Score:-4.04|P-value:2.72E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP1(YER047C)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SAW1(YAL027W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SHE10(YGL228W)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SLM6(YBR266C_d)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SPT2(YER161C)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SWC5(YBR231C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TFB5(YDR079C-A)|FD-Score:4.88|P-value:5.24E-7||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TRM1(YDR120C)|FD-Score:6.02|P-value:8.78E-10||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TSC3(YBR058C-A)|FD-Score:10.1|P-value:2.00E-24||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:4.51|P-value:3.23E-6||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAB2(YEL005C)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VMA13(YPR036W)|FD-Score:-6.03|P-value:7.96E-10||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA9(YCL005W-A)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:WTM1(YOR230W)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YCR007C(YCR007C_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR220C(YDR220C_d)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YDR442W(YDR442W_d)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER175W-A(YER175W-A_p)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR121W-A(YGR121W-A_p)|FD-Score:5.64|P-value:8.51E-9||SGD DESC:Putative protein of unknown function Gene:YHR022C-A(YHR022C-A_p)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJR039W(YJR039W_p)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR001C(YLR001C_p)|FD-Score:8.4|P-value:2.19E-17||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR053C(YLR053C_p)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Putative protein of unknown function Gene:YLR072W(YLR072W_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YNR029C(YNR029C_p)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR186W(YOR186W_p)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR289W(YOR289W_p)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YOR309C(YOR309C_d)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR1(YDR368W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:7.39|P-value:7.57E-14||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRO2(YBR054W)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication Gene:ADE6(YGR061C)|FD-Score:4.29|P-value:9.02E-6||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARG82(YDR173C)|FD-Score:4.85|P-value:6.29E-7||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG7(YHR171W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BOI1(YBL085W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:12.9|P-value:3.47E-38||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CIN2(YPL241C)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLB6(YGR109C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CTF18(YMR078C)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CUS2(YNL286W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DUR3(YHL016C)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ERT1(YBR239C_p)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:ERV14(YGL054C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ETR1(YBR026C)|FD-Score:-4.72|P-value:1.17E-6||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FDC1(YDR539W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FIR1(YER032W)|FD-Score:-3.99|P-value:3.27E-5||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FLO11(YIR019C)|FD-Score:6.01|P-value:9.26E-10||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FRD1(YEL047C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FRE3(YOR381W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FRE6(YLL051C)|FD-Score:4.81|P-value:7.55E-7||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GIR2(YDR152W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GIS4(YML006C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:GLE2(YER107C)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GUP1(YGL084C)|FD-Score:-6.28|P-value:1.65E-10||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GYP1(YOR070C)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBS1(YKR084C)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HER2(YMR293C)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HIS2(YFR025C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HSP26(YBR072W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HSP31(YDR533C)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HST1(YOL068C)|FD-Score:5.62|P-value:9.39E-9||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IOC3(YFR013W)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC4(YDR540C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:ISU2(YOR226C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KRE6(YPR159W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LTE1(YAL024C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MHO1(YJR008W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MNT3(YIL014W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MOT2(YER068W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:NBP2(YDR162C)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NTG1(YAL015C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:OCT1(YKL134C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OXR1(YPL196W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PBP2(YBR233W)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PCI8(YIL071C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:POL32(YJR043C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:POP2(YNR052C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PTC7(YHR076W)|FD-Score:-4.58|P-value:2.35E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTR2(YKR093W)|FD-Score:-4.57|P-value:2.39E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAD6(YGL058W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RCE1(YMR274C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RIM11(YMR139W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RME1(YGR044C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPA12(YJR063W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL19A(YBR084C-A)|FD-Score:4.31|P-value:8.00E-6||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:4.28|P-value:9.43E-6||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RTT106(YNL206C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAC1(YKL212W)|FD-Score:-4.04|P-value:2.72E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP1(YER047C)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SAW1(YAL027W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SHE10(YGL228W)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SLM6(YBR266C_d)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SPT2(YER161C)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SWC5(YBR231C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TFB5(YDR079C-A)|FD-Score:4.88|P-value:5.24E-7||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TRM1(YDR120C)|FD-Score:6.02|P-value:8.78E-10||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TSC3(YBR058C-A)|FD-Score:10.1|P-value:2.00E-24||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:4.51|P-value:3.23E-6||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAB2(YEL005C)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VMA13(YPR036W)|FD-Score:-6.03|P-value:7.96E-10||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA9(YCL005W-A)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:WTM1(YOR230W)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YCR007C(YCR007C_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR220C(YDR220C_d)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YDR442W(YDR442W_d)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER175W-A(YER175W-A_p)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR121W-A(YGR121W-A_p)|FD-Score:5.64|P-value:8.51E-9||SGD DESC:Putative protein of unknown function Gene:YHR022C-A(YHR022C-A_p)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJR039W(YJR039W_p)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR001C(YLR001C_p)|FD-Score:8.4|P-value:2.19E-17||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR053C(YLR053C_p)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Putative protein of unknown function Gene:YLR072W(YLR072W_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YNR029C(YNR029C_p)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR186W(YOR186W_p)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR289W(YOR289W_p)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YOR309C(YOR309C_d)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR1(YDR368W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:7.39|P-value:7.57E-14||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRO2(YBR054W)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL019W6.261.97E-101.33MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YJL050W6.009.83E-101.33MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YPR019W4.671.50E-60.09MCM4Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
YLR066W4.582.28E-60.23SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YHR062C4.356.66E-60.09RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR098C4.261.00E-50.20NUP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
YPL238C_d4.062.43E-50.04YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YDL217C4.022.91E-50.11TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YFR051C3.914.58E-50.02RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YPR137W3.894.91E-50.04RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YNL244C3.865.75E-50.09SUI1Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
YLL004W3.778.14E-50.06ORC3Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YFL024C3.711.02E-40.02EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YGL093W3.701.10E-40.39SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YGL073W3.314.66E-40.06HSF1Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL069C12.903.47E-38BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YBR058C-A10.102.00E-24TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YLR001C_p8.402.19E-17YLR001C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
YLR262C7.397.57E-14YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YDR120C6.028.78E-10TRM1tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
YIR019C6.019.26E-10FLO11GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain
YGR121W-A_p5.648.51E-9YGR121W-A_pPutative protein of unknown function
YOL068C5.629.39E-9HST1NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YBR054W5.131.43E-7YRO2Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication
YPL241C5.091.77E-7CIN2GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
YDR079C-A4.885.24E-7TFB5Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair
YDR173C4.856.29E-7ARG82Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
YLL051C4.817.55E-7FRE6Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
YML124C4.513.23E-6TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YBR084C-A4.318.00E-6RPL19ARibosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2483
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2436.06E-80SGTC_24875918373 44.0 μMMiscellaneous28762790.763636
0.2032.26E-55SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651801
0.1991.24E-53SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0526316TSC3-RPN4
0.1891.31E-48SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.138889TSC3-RPN4
0.1862.22E-47SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.182927TSC3-RPN4
0.1844.74E-46SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.140845
0.1802.24E-44SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0657895TSC3-RPN4
0.1782.52E-43SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.373333TSC3-RPN4
0.1775.62E-43SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.097561TSC3-RPN4
0.1689.29E-39SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.103896TSC3-RPN4
0.1624.59E-36SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.158537
0.1611.10E-35SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0789474TSC3-RPN4
0.1602.49E-35SGTC_6390833-0042 114.0 μMChemDiv (Drug-like library)59789110.103896
0.1602.92E-35SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.060241TSC3-RPN4
0.1597.11E-35SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.133333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2103551-044556.83 μM12865180ChemDiv (Drug-like library)371.406983.0316
SGTC_2465580924233.21 μM0.7924532731748Miscellaneous357.38042.68116
SGTC_2487591837344.05 μM0.7636362876279Miscellaneous385.39052.46517
SGTC_2469565501990.81 μM0.7358492788239Miscellaneous341.3813.18315
SGTC_2479561735343.85 μM0.725492731746Miscellaneous327.354422.69715
SGTC_2458580890716.39 μM0.7037042731747Miscellaneous361.799483.36215
SGTC_2461575530226.15 μM0.7037042788904Miscellaneous406.250483.44615
SGTC_247358545938.94 μM0.6666672872951Miscellaneous410.486083.77716
SGTC_2456577035974.46 μM0.6440683118787Miscellaneous412.45892.54717
SGTC_2466598340937.09 μM0.5862072879657Miscellaneous345.3448832.90316