Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
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YLR208W | 15.50 | 1.84E-54 | 11.00 | SEC13 | Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress |
YFL039C | 4.51 | 3.31E-6 | 0.33 | ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions |
YNL132W | 4.18 | 1.45E-5 | 0.12 | KRE33 | Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance |
YDR172W | 4.07 | 2.39E-5 | 0.02 | SUP35 | Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype |
YFL022C | 4.04 | 2.62E-5 | 0.04 | FRS2 | Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar |
YER133W | 4.01 | 3.08E-5 | 0.01 | GLC7 | Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest |
YIL106W | 4.00 | 3.19E-5 | 0.23 | MOB1 | Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress |
YCL059C | 3.77 | 8.04E-5 | 0.03 | KRR1 | Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit |
YJL076W | 3.75 | 8.94E-5 | 0.14 | NET1 | Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication |
YPR041W | 3.60 | 1.58E-4 | 0.04 | TIF5 | Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 |
YLR317W_d | 3.56 | 1.84E-4 | 0.08 | YLR317W_d | Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C |
YBL035C | 3.48 | 2.51E-4 | 0.04 | POL12 | B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation |
YLL004W | 3.44 | 2.94E-4 | 0.05 | ORC3 | Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing |
YNL251C | 3.38 | 3.58E-4 | 0.01 | NRD1 | RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites |
YBR070C | 3.37 | 3.70E-4 | 0.02 | ALG14 | Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases |