carminic acid

3,5,6,8-tetrahydroxy-1-methyl-9,10-dioxo-7-[(2R,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]anthracene-2-carboxylic acid

Coloring matter from the insect Coccus cacti L. It is used in foods, pharmaceuticals, toiletries, etc., as a dye, and also has use as a microscopic stain and biological marker.

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PubChem MeSH terms: Coloring Agents;Food Coloring Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2490
Screen concentration 95.4 μM
Source TimTec (Pure natural product library)
PubChem CID 14749
SMILES CC1=C2C(=CC(=C1C(=O)O)O)C(=O)C3=C(C2=O)C(=C(C(=C3O)O)C4C(C(C(C(O4)CO)O)O)O)O
Standardized SMILES Cc1c(C(=O)O)c(O)cc2C(=O)c3c(O)c(O)c(C4OC(CO)C(O)C(O)C4O)c(O)c3C(=O)c12
Molecular weight 492.3864
ALogP -0.44
H-bond donor count 9
H-bond acceptor count 13
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.52
% growth inhibition (Hom. pool) 6.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 14749
Download HIP data (tab-delimited text)  (excel)
Gene:BRN1(YBL097W)|FD-Score:5.04|P-value:2.29E-7|Clearance:0.07||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:GLC7(YER133W)|FD-Score:-3.75|P-value:8.72E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MEX67(YPL169C)|FD-Score:-5.51|P-value:1.78E-8|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MGE1(YOR232W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.15||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:MTR10(YOR160W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.12||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR4(YJL050W)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.46||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NIC96(YFR002W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NMD3(YHR170W)|FD-Score:-3.27|P-value:5.28E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RRP12(YPL012W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.37||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:RSC58(YLR033W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC23(YPR181C)|FD-Score:-3.14|P-value:8.48E-4|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SSL1(YLR005W)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.22||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SUI1(YNL244C)|FD-Score:4.29|P-value:8.81E-6|Clearance:0.14||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TOM40(YMR203W)|FD-Score:4.51|P-value:3.22E-6|Clearance:0.22||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRR1(YDR353W)|FD-Score:6.97|P-value:1.61E-12|Clearance:1.93||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:YDL196W(YDL196W_d)|FD-Score:4.97|P-value:3.35E-7|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:BRN1(YBL097W)|FD-Score:5.04|P-value:2.29E-7|Clearance:0.07||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:GLC7(YER133W)|FD-Score:-3.75|P-value:8.72E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MEX67(YPL169C)|FD-Score:-5.51|P-value:1.78E-8|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MGE1(YOR232W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.15||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:MTR10(YOR160W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.12||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR4(YJL050W)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.46||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NIC96(YFR002W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NMD3(YHR170W)|FD-Score:-3.27|P-value:5.28E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RRP12(YPL012W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.37||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:RSC58(YLR033W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC23(YPR181C)|FD-Score:-3.14|P-value:8.48E-4|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SSL1(YLR005W)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.22||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SUI1(YNL244C)|FD-Score:4.29|P-value:8.81E-6|Clearance:0.14||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TOM40(YMR203W)|FD-Score:4.51|P-value:3.22E-6|Clearance:0.22||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRR1(YDR353W)|FD-Score:6.97|P-value:1.61E-12|Clearance:1.93||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:YDL196W(YDL196W_d)|FD-Score:4.97|P-value:3.35E-7|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 14749
Download HOP data (tab-delimited text)  (excel)
Gene:ATX1(YNL259C)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BRE1(YDL074C)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CDC10(YCR002C)|FD-Score:-3.99|P-value:3.24E-5||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CNL1(YDR357C_p)|FD-Score:4.44|P-value:4.53E-6||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:FLO8(YER109C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:GCR2(YNL199C)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GOT1(YMR292W)|FD-Score:-3.83|P-value:6.54E-5||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GRX3(YDR098C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:HEF3(YNL014W)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:IGD1(YFR017C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:IMG1(YCR046C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:ISM1(YPL040C)|FD-Score:-3.85|P-value:5.80E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LYS5(YGL154C)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine Gene:MHR1(YDR296W)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MIC17(YMR002W)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNT3(YIL014W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MRH4(YGL064C)|FD-Score:-4.7|P-value:1.31E-6||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:OCT1(YKL134C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PMU1(YKL128C)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PRB1(YEL060C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PRO2(YOR323C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRY2(YKR013W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RIM8(YGL045W)|FD-Score:5.18|P-value:1.08E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RNY1(YPL123C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPA12(YJR063W)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL6B(YLR448W)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:SNO4(YMR322C_p)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation Gene:THP1(YOL072W)|FD-Score:-4.77|P-value:9.39E-7||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:YDR029W(YDR029W_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR182C(YGR182C_d)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W Gene:YHR131C(YHR131C_p)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YJL120W(YJL120W_d)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL163C(YJL163C_p)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function Gene:YKL169C(YKL169C_d)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR053C(YLR053C_p)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Putative protein of unknown function Gene:YOL160W(YOL160W_d)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:4.32|P-value:7.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YTA12(YMR089C)|FD-Score:-4.01|P-value:3.03E-5||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:ATX1(YNL259C)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BRE1(YDL074C)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CDC10(YCR002C)|FD-Score:-3.99|P-value:3.24E-5||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CNL1(YDR357C_p)|FD-Score:4.44|P-value:4.53E-6||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:FLO8(YER109C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:GCR2(YNL199C)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GOT1(YMR292W)|FD-Score:-3.83|P-value:6.54E-5||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GRX3(YDR098C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:HEF3(YNL014W)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:IGD1(YFR017C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:IMG1(YCR046C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:ISM1(YPL040C)|FD-Score:-3.85|P-value:5.80E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LYS5(YGL154C)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine Gene:MHR1(YDR296W)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MIC17(YMR002W)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNT3(YIL014W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MRH4(YGL064C)|FD-Score:-4.7|P-value:1.31E-6||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:OCT1(YKL134C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PMU1(YKL128C)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PRB1(YEL060C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PRO2(YOR323C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRY2(YKR013W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RIM8(YGL045W)|FD-Score:5.18|P-value:1.08E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RNY1(YPL123C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPA12(YJR063W)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL6B(YLR448W)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:SNO4(YMR322C_p)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation Gene:THP1(YOL072W)|FD-Score:-4.77|P-value:9.39E-7||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:YDR029W(YDR029W_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR182C(YGR182C_d)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W Gene:YHR131C(YHR131C_p)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YJL120W(YJL120W_d)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL163C(YJL163C_p)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function Gene:YKL169C(YKL169C_d)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR053C(YLR053C_p)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Putative protein of unknown function Gene:YOL160W(YOL160W_d)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:4.32|P-value:7.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YTA12(YMR089C)|FD-Score:-4.01|P-value:3.03E-5||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR353W6.971.61E-121.93TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
YBL097W5.042.29E-70.07BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YDL196W_d4.973.35E-70.24YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YLR005W4.731.10E-60.22SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YMR203W4.513.22E-60.22TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YNL244C4.298.81E-60.14SUI1Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
YJL050W4.161.61E-50.46MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YOR160W3.691.10E-40.12MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YLR033W3.581.73E-40.01RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YOR232W3.571.80E-40.15MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YPL012W3.423.14E-40.37RRP12Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
YLR105C3.050.001140.02SEN2Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
YER003C3.030.001230.07PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YLR147C2.960.001540.10SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YLR066W2.860.002130.01SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL045W5.181.08E-7RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
YNL259C4.671.53E-6ATX1Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake
YKL128C4.572.44E-6PMU1Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant
YDR357C_p4.444.53E-6CNL1_pProtein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YPR123C_d4.327.70E-6YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YMR322C_p4.062.46E-5SNO4_pPossible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
YGL154C3.807.21E-5LYS5Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine
YOR323C3.701.07E-4PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YDR098C3.551.91E-4GRX3Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36
YDR029W_d3.502.35E-4YDR029W_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
YGR182C_d3.423.16E-4YGR182C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W
YOL160W_d3.403.32E-4YOL160W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL123C3.393.49E-4RNY1Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity
YER109C3.334.33E-4FLO8Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene
YFR017C3.275.42E-4IGD1Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2490
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2441.50E-80SGTC_2492derrusnin 100.0 μMMicrosource (Natural product library)17153070.0909091
0.1825.70E-45SGTC_2493resveratrol 26.1 μMMicrosource (Natural product library)62554620.084507
0.1681.66E-38SGTC_2491paeonol 100.0 μMMicrosource (Natural product library)110920.147059
0.1549.66E-33SGTC_31619100388 49.5 μMChembridge (Drug-like library)252363680.0674157iron homeostasis
0.1429.13E-28SGTC_2586curcumin 80.0 μMICCB bioactive library66103320.129412iron homeostasis
0.1352.17E-25SGTC_31759106517 49.5 μMChembridge (Drug-like library)414680730.0833333iron homeostasis
0.1315.39E-24SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.097561iron homeostasis
0.1279.03E-23SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.0588235heme biosynthesis & mitochondrial translocase
0.1224.23E-21SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.0875heme biosynthesis & mitochondrial translocase
0.1141.75E-18SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.117647heme biosynthesis & mitochondrial translocase
0.1103.01E-17SGTC_3393091-4833 71.4 μMChemDiv (Drug-like library)32400770.0864198iron homeostasis
0.1089.32E-17SGTC_2675theaflavin digallate 100.0 μMMicrosource (Natural product library)4673200.168421iron homeostasis
0.1056.56E-16SGTC_23447990960 77.0 μMChembridge (Fragment library)1408130.0571429
0.1032.29E-15SGTC_29289011484 9.8 μMChembridge (Drug-like library)29947510.0689655heme biosynthesis & mitochondrial translocase
0.1001.67E-14SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0526316heme requiring

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1569carminic acid40.6 μM114749TimTec (Pure natural product library)492.3864-0.436913
SGTC_2597laccaic acid a100 μM0.365491415Microsource (Natural product library)537.428561.824812
SGTC_2410aloe-emodin-8-o-glycoside46.3 μM0.33766214077415TimTec (Pure natural product library)432.3775-0.21610mitochondrial processes
SGTC_1956st07708554 μM0.27848124208685TimTec (Natural product derivative library)370.30812-0.531510
SGTC_2611pyromycin23.93 μM0.265306196990Microsource (Natural product library)585.5991.992512
SGTC_1530rhein70.4 μM0.25373110168TimTec (Pure natural product library)284.220421.95436
SGTC_272doxorubicin8.13 μM0.25252531703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_2747doxorubicin7.63 μM0.25252531703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_1085doxorubicin7.67 μM0.252525443939NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair
SGTC_1105epirubicin18.1 μM0.25252565348NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair
SGTC_1079idarubicin2.36 μM0.241758636362NIH Clinical Collection533.954821.026610DNA damage response
SGTC_248streptozotocin5.23 mM0.2394375300Miscellaneous265.2206-2.28558DNA damage response