avocadenofuran

2-tridec-12-enylfuran

A insecticide and lysyl oxidase inhibitor.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2499
Screen concentration 51.5 μM
Source Microsource (Natural product library)
PubChem CID 6857792
SMILES C=CCCCCCCCCCCCC1=CC=CO1
Standardized SMILES C=CCCCCCCCCCCCc1occc1
Molecular weight 248.4036
ALogP 6.37
H-bond donor count 0
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.57
% growth inhibition (Hom. pool) 8.52


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6857792
Download HIP data (tab-delimited text)  (excel)
Gene:BRR6(YGL247W)|FD-Score:3.24|P-value:6.02E-4|Clearance:0.08||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:BUR6(YER159C)|FD-Score:3.48|P-value:2.46E-4|Clearance:0.18||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CCT3(YJL014W)|FD-Score:-6.47|P-value:4.91E-11|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:-3.87|P-value:5.45E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC11(YJR076C)|FD-Score:4.41|P-value:5.15E-6|Clearance:0.58||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CWC2(YDL209C)|FD-Score:-3.44|P-value:2.96E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GWT1(YJL091C)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:MCD4(YKL165C)|FD-Score:3.3|P-value:4.78E-4|Clearance:0.05||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NAF1(YNL124W)|FD-Score:-3.2|P-value:6.87E-4|Clearance:0||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:PRE1(YER012W)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.19||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE2(YPR103W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.2||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRP16(YKR086W)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.01||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:RPP0(YLR340W)|FD-Score:3.83|P-value:6.51E-5|Clearance:0.14||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC24(YIL109C)|FD-Score:3.25|P-value:5.71E-4|Clearance:0.02||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SPT14(YPL175W)|FD-Score:5.32|P-value:5.24E-8|Clearance:0.91||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SSY1(YDR160W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.03||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TCP1(YDR212W)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:BRR6(YGL247W)|FD-Score:3.24|P-value:6.02E-4|Clearance:0.08||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:BUR6(YER159C)|FD-Score:3.48|P-value:2.46E-4|Clearance:0.18||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CCT3(YJL014W)|FD-Score:-6.47|P-value:4.91E-11|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:-3.87|P-value:5.45E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC11(YJR076C)|FD-Score:4.41|P-value:5.15E-6|Clearance:0.58||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CWC2(YDL209C)|FD-Score:-3.44|P-value:2.96E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GWT1(YJL091C)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:MCD4(YKL165C)|FD-Score:3.3|P-value:4.78E-4|Clearance:0.05||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NAF1(YNL124W)|FD-Score:-3.2|P-value:6.87E-4|Clearance:0||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:PRE1(YER012W)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.19||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE2(YPR103W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.2||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRP16(YKR086W)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.01||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:RPP0(YLR340W)|FD-Score:3.83|P-value:6.51E-5|Clearance:0.14||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC24(YIL109C)|FD-Score:3.25|P-value:5.71E-4|Clearance:0.02||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SPT14(YPL175W)|FD-Score:5.32|P-value:5.24E-8|Clearance:0.91||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SSY1(YDR160W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.03||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TCP1(YDR212W)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6857792
Download HOP data (tab-delimited text)  (excel)
Gene:AFT2(YPL202C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ARD1(YHR013C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARF1(YDL192W)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARK1(YNL020C)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ASG7(YJL170C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATP11(YNL315C)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP4(YPL078C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BMH1(YER177W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BTS1(YPL069C)|FD-Score:-6.9|P-value:2.60E-12||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CKA2(YOR061W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COG7(YGL005C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX17(YLL009C)|FD-Score:-4.3|P-value:8.58E-6||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CTH1(YDR151C)|FD-Score:6.05|P-value:7.07E-10||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:DAL81(YIR023W)|FD-Score:7.12|P-value:5.46E-13||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCG1(YIR030C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DST1(YGL043W)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM14(YHR132C)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ECM5(YMR176W)|FD-Score:-5.69|P-value:6.48E-9||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EMC4(YGL231C)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERV14(YGL054C)|FD-Score:6.88|P-value:2.94E-12||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FBP1(YLR377C)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FMC1(YIL098C)|FD-Score:5.77|P-value:4.00E-9||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GCN5(YGR252W)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GDH3(YAL062W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIN4(YDR507C)|FD-Score:5.8|P-value:3.32E-9||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GIP3(YPL137C)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GOS1(YHL031C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPM3(YOL056W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GRX4(YER174C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HOC1(YJR075W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HTZ1(YOL012C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IMG2(YCR071C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRC4(YDR540C)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IVY1(YDR229W)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:KAR9(YPL269W)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KEL2(YGR238C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LRO1(YNR008W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MET31(YPL038W)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MRP4(YHL004W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MUP3(YHL036W)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NIP100(YPL174C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NPL4(YBR170C)|FD-Score:-4.85|P-value:6.23E-7||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:PEP12(YOR036W)|FD-Score:4.37|P-value:6.13E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:4.36|P-value:6.58E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET100(YDR079W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PHO80(YOL001W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO88(YBR106W)|FD-Score:4.83|P-value:6.69E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:POP2(YNR052C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRD1(YCL057W)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PRS3(YHL011C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK2(YJR059W)|FD-Score:5.98|P-value:1.12E-9||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RCO1(YMR075W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:REG1(YDR028C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIT1(YMR283C)|FD-Score:4.88|P-value:5.31E-7||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RKR1(YMR247C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RMD8(YFR048W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Cytosolic protein required for sporulation Gene:ROY1(YMR258C)|FD-Score:3.92|P-value:4.52E-5||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPN4(YDL020C)|FD-Score:24.4|P-value:4.10E-132||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT12(YCR045C)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSM22(YKL155C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTT106(YNL206C)|FD-Score:6.53|P-value:3.20E-11||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAC6(YDR129C)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM1(YLR180W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SEC66(YBR171W)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFB3(YHR098C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHE10(YGL228W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SPH1(YLR313C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPO11(YHL022C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPO7(YAL009W)|FD-Score:5.15|P-value:1.34E-7||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SRC1(YML034W)|FD-Score:4|P-value:3.10E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSN2(YDR443C)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSN8(YNL025C)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STI1(YOR027W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SUR4(YLR372W)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYF2(YGR129W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:THP1(YOL072W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TPN1(YGL186C)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:26.3|P-value:4.04E-153||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP13(YBL067C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VBA4(YDR119W_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:YAP1802(YGR241C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBR178W(YBR178W_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR023C(YCR023C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL032W(YDL032W_d)|FD-Score:5.62|P-value:9.59E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEL045C(YEL045C_d)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGL072C(YGL072C_d)|FD-Score:5.12|P-value:1.50E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR259C(YGR259C_d)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YHL026C(YHL026C_p)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YHR210C(YHR210C_p)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIR042C(YIR042C_p)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YLR126C(YLR126C_p)|FD-Score:-5.05|P-value:2.24E-7||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YMR085W(YMR085W_p)|FD-Score:3.5|P-value:2.28E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR105W-A(YMR105W-A_p)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Putative protein of unknown function Gene:YNL235C(YNL235C_d)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YPR014C(YPR014C_d)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:AFT2(YPL202C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ARD1(YHR013C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARF1(YDL192W)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARK1(YNL020C)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ASG7(YJL170C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATP11(YNL315C)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP4(YPL078C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BMH1(YER177W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BTS1(YPL069C)|FD-Score:-6.9|P-value:2.60E-12||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CKA2(YOR061W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COG7(YGL005C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX17(YLL009C)|FD-Score:-4.3|P-value:8.58E-6||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CTH1(YDR151C)|FD-Score:6.05|P-value:7.07E-10||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:DAL81(YIR023W)|FD-Score:7.12|P-value:5.46E-13||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCG1(YIR030C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DST1(YGL043W)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM14(YHR132C)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ECM5(YMR176W)|FD-Score:-5.69|P-value:6.48E-9||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EMC4(YGL231C)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERV14(YGL054C)|FD-Score:6.88|P-value:2.94E-12||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FBP1(YLR377C)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FMC1(YIL098C)|FD-Score:5.77|P-value:4.00E-9||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GCN5(YGR252W)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GDH3(YAL062W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIN4(YDR507C)|FD-Score:5.8|P-value:3.32E-9||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GIP3(YPL137C)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GOS1(YHL031C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPM3(YOL056W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GRX4(YER174C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HOC1(YJR075W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HTZ1(YOL012C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IMG2(YCR071C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRC4(YDR540C)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IVY1(YDR229W)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:KAR9(YPL269W)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KEL2(YGR238C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LRO1(YNR008W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MET31(YPL038W)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MRP4(YHL004W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MUP3(YHL036W)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NIP100(YPL174C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NPL4(YBR170C)|FD-Score:-4.85|P-value:6.23E-7||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:PEP12(YOR036W)|FD-Score:4.37|P-value:6.13E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:4.36|P-value:6.58E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET100(YDR079W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PHO80(YOL001W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO88(YBR106W)|FD-Score:4.83|P-value:6.69E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:POP2(YNR052C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRD1(YCL057W)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PRS3(YHL011C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK2(YJR059W)|FD-Score:5.98|P-value:1.12E-9||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RCO1(YMR075W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:REG1(YDR028C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIT1(YMR283C)|FD-Score:4.88|P-value:5.31E-7||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RKR1(YMR247C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RMD8(YFR048W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Cytosolic protein required for sporulation Gene:ROY1(YMR258C)|FD-Score:3.92|P-value:4.52E-5||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPN4(YDL020C)|FD-Score:24.4|P-value:4.10E-132||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT12(YCR045C)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSM22(YKL155C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTT106(YNL206C)|FD-Score:6.53|P-value:3.20E-11||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAC6(YDR129C)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM1(YLR180W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SEC66(YBR171W)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFB3(YHR098C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHE10(YGL228W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SPH1(YLR313C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPO11(YHL022C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPO7(YAL009W)|FD-Score:5.15|P-value:1.34E-7||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SRC1(YML034W)|FD-Score:4|P-value:3.10E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSN2(YDR443C)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSN8(YNL025C)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STI1(YOR027W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SUR4(YLR372W)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYF2(YGR129W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:THP1(YOL072W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TPN1(YGL186C)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:26.3|P-value:4.04E-153||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP13(YBL067C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VBA4(YDR119W_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:YAP1802(YGR241C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBR178W(YBR178W_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR023C(YCR023C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL032W(YDL032W_d)|FD-Score:5.62|P-value:9.59E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEL045C(YEL045C_d)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGL072C(YGL072C_d)|FD-Score:5.12|P-value:1.50E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR259C(YGR259C_d)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YHL026C(YHL026C_p)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YHR210C(YHR210C_p)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIR042C(YIR042C_p)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YLR126C(YLR126C_p)|FD-Score:-5.05|P-value:2.24E-7||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YMR085W(YMR085W_p)|FD-Score:3.5|P-value:2.28E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR105W-A(YMR105W-A_p)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Putative protein of unknown function Gene:YNL235C(YNL235C_d)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YPR014C(YPR014C_d)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL175W5.325.24E-80.91SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YJR076C4.415.15E-60.58CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YLR340W3.836.51E-50.14RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YPR103W3.691.13E-40.20PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YKR086W3.492.41E-40.01PRP16DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step
YER159C3.482.46E-40.18BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YKL165C3.304.78E-40.05MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YIL109C3.255.71E-40.02SEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
YGL247W3.246.02E-40.08BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YDR160W3.167.94E-40.03SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YER012W3.138.71E-40.19PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YKL173W2.940.001650.07SNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
YDL029W2.870.002050.03ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YJL034W2.840.002230.03KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YLL018C2.820.002420.12DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A26.304.04E-153TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDL020C24.404.10E-132RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YIR023W7.125.46E-13DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YGL054C6.882.94E-12ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YNL206C6.533.20E-11RTT106Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition
YGL043W6.311.40E-10DST1General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
YDR151C6.057.07E-10CTH1Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis
YJR059W5.981.12E-9PTK2Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
YDR507C5.803.32E-9GIN4Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication
YIL098C5.774.00E-9FMC1Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p
YDL032W_d5.629.59E-9YDL032W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene
YPL174C5.161.22E-7NIP100Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
YAL009W5.151.34E-7SPO7Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
YNL025C5.131.47E-7SSN8Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance
YGL072C_d5.121.50E-7YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2

GO enrichment analysis for SGTC_2499
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4650SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0909091TSC3-RPN4
0.4436.01E-283SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0980392
0.4225.26E-254SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.056338TSC3-RPN4
0.4214.13E-252SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0892857TSC3-RPN4
0.4201.16E-251SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0980392
0.4206.00E-251SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.107143
0.4192.65E-249SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0714286TSC3-RPN4
0.3951.87E-219SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0677966TSC3-RPN4
0.3894.12E-212SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0735294
0.3861.33E-209SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0357143TSC3-RPN4
0.3821.59E-204SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0545455TSC3-RPN4
0.3742.48E-195SGTC_2740felodipine 17.1 μMMiscellaneous33330.0769231
0.3701.38E-191SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0980392TSC3-RPN4
0.3643.37E-184SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0793651TSC3-RPN4
0.3531.50E-172SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.0793651

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_9322857-0803154 μM0.282609674898ChemDiv (Drug-like library)280.11732.55112
SGTC_1649st01173751.3 μM0.2777781761035TimTec (Natural product derivative library)289.455545.21212
SGTC_22206782180200 μM0.270833833803Chembridge (Fragment library)207.18606-0.26124RNA processing & uracil transport
SGTC_2442515890648.48 μM0.2692311893835Miscellaneous251.324663.05904
SGTC_2524avocatin b10.57 μM0.2682936710748Microsource (Natural product library)570.884248.94166
SGTC_2944906734625.62 μM0.2653066473075Chembridge (Drug-like library)324.20184.1723
SGTC_22717849410118.62 μM0.26972430Chembridge (Fragment library)233.289542.66623
SGTC_22737815444169.05 μM0.2549022824616Chembridge (Fragment library)228.268182.00424
SGTC_5264469-0568170 μM0.252901964ChemDiv (Drug-like library)257.310942.19715azole & statin
SGTC_7754577-058057.3 μM0.2452832006902ChemDiv (Drug-like library)376.289162.86815