0118-0223

5-methyl-4-[2-(4-oxocyclohexa-2,5-dien-1-ylidene)hydrazinyl]-2-phenyl-1H-pyrazol-3-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_25
Screen concentration 35.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 5333628
SMILES CC1=C(C(=O)N(N1)C2=CC=CC=C2)NN=C3C=CC(=O)C=C3
Standardized SMILES Cc1nn(c(O)c1N=Nc2ccc(O)cc2)c3ccccc3
Molecular weight 294.308
ALogP 0.92
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 36.11
% growth inhibition (Hom. pool) 14.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5333628
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:5.06|P-value:2.15E-7|Clearance:0.82||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:COG3(YER157W)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIS3(YOL021C)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.55||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:MMS21(YEL019C)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.05||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NOP15(YNL110C)|FD-Score:3.29|P-value:4.94E-4|Clearance:0.04||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RIB5(YBR256C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.39||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:SEC1(YDR164C)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SRB4(YER022W)|FD-Score:3.26|P-value:5.63E-4|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:CCT2(YIL142W)|FD-Score:5.06|P-value:2.15E-7|Clearance:0.82||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:COG3(YER157W)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIS3(YOL021C)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.55||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:MMS21(YEL019C)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.05||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NOP15(YNL110C)|FD-Score:3.29|P-value:4.94E-4|Clearance:0.04||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RIB5(YBR256C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.39||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:SEC1(YDR164C)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SRB4(YER022W)|FD-Score:3.26|P-value:5.63E-4|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5333628
Download HOP data (tab-delimited text)  (excel)
Gene:ADE12(YNL220W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:ADE3(YGR204W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADO1(YJR105W)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ANS1(YHR126C_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARK1(YNL020C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ATG3(YNR007C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CIN5(YOR028C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CTR9(YOL145C)|FD-Score:4.8|P-value:7.97E-7||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CYS3(YAL012W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DEF1(YKL054C)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DIF1(YLR437C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DSE1(YER124C)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM14(YHR132C)|FD-Score:-3.19|P-value:7.23E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ENV10(YLR065C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERV14(YGL054C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAA4(YMR246W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FEN1(YCR034W)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FYV1(YDR024W_d)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GIT1(YCR098C)|FD-Score:4.51|P-value:3.20E-6||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:HTL1(YCR020W-B)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:INM1(YHR046C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:LRO1(YNR008W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LYS14(YDR034C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:LYS4(YDR234W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MDM32(YOR147W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MDS3(YGL197W)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MET22(YOL064C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MRE11(YMR224C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRT4(YKL009W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSH2(YOL090W)|FD-Score:-4.92|P-value:4.31E-7||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NHA1(YLR138W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:PFK2(YMR205C)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIG2(YIL045W)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PRO2(YOR323C)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RHO2(YNL090W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RMD8(YFR048W)|FD-Score:-3.78|P-value:7.87E-5||SGD DESC:Cytosolic protein required for sporulation Gene:RPL18B(YNL301C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL20B(YOR312C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:5.85|P-value:2.53E-9||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS30A(YLR287C-A)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SBE2(YDR351W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth Gene:SEY1(YOR165W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFB3(YHR098C)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SFM1(YOR021C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SGT2(YOR007C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SSE1(YPL106C)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:STB5(YHR178W)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SWT21(YNL187W)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TRM11(YOL124C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TRP1(YDR007W)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSR3(YOR006C)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:UBP1(YDL122W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VBA2(YBR293W)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS24(YKL041W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS52(YDR484W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YDR008C(YDR008C_d)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:-4.59|P-value:2.25E-6||SGD DESC:Putative protein of unknown function Gene:YHC3(YJL059W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YJR015W(YJR015W_p)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YKR073C(YKR073C_d)|FD-Score:4.32|P-value:7.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR084W(YMR084W_p)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL109W(YNL109W_d)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YOL019W(YOL019W_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOL029C(YOL029C_p)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YOL079W(YOL079W_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL153C(YOL153C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Hypothetical protein Gene:YOR114W(YOR114W_p)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL260W(YPL260W_p)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR114W(YPR114W_p)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function Gene:YPR197C(YPR197C_d)|FD-Score:5.38|P-value:3.75E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADE12(YNL220W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:ADE3(YGR204W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADO1(YJR105W)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ANS1(YHR126C_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARK1(YNL020C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ATG3(YNR007C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CIN5(YOR028C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CTR9(YOL145C)|FD-Score:4.8|P-value:7.97E-7||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CYS3(YAL012W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DEF1(YKL054C)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DIF1(YLR437C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DSE1(YER124C)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM14(YHR132C)|FD-Score:-3.19|P-value:7.23E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ENV10(YLR065C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERV14(YGL054C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAA4(YMR246W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FEN1(YCR034W)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FYV1(YDR024W_d)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GIT1(YCR098C)|FD-Score:4.51|P-value:3.20E-6||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:HTL1(YCR020W-B)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:INM1(YHR046C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:LRO1(YNR008W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LYS14(YDR034C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:LYS4(YDR234W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MDM32(YOR147W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MDS3(YGL197W)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MET22(YOL064C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MRE11(YMR224C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRT4(YKL009W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSH2(YOL090W)|FD-Score:-4.92|P-value:4.31E-7||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NHA1(YLR138W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:PFK2(YMR205C)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIG2(YIL045W)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PRO2(YOR323C)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RHO2(YNL090W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RMD8(YFR048W)|FD-Score:-3.78|P-value:7.87E-5||SGD DESC:Cytosolic protein required for sporulation Gene:RPL18B(YNL301C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL20B(YOR312C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:5.85|P-value:2.53E-9||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS30A(YLR287C-A)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SBE2(YDR351W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth Gene:SEY1(YOR165W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFB3(YHR098C)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SFM1(YOR021C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SGT2(YOR007C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SSE1(YPL106C)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:STB5(YHR178W)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SWT21(YNL187W)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TRM11(YOL124C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TRP1(YDR007W)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSR3(YOR006C)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:UBP1(YDL122W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VBA2(YBR293W)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS24(YKL041W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS52(YDR484W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YDR008C(YDR008C_d)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:-4.59|P-value:2.25E-6||SGD DESC:Putative protein of unknown function Gene:YHC3(YJL059W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YJR015W(YJR015W_p)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YKR073C(YKR073C_d)|FD-Score:4.32|P-value:7.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR084W(YMR084W_p)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL109W(YNL109W_d)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YOL019W(YOL019W_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOL029C(YOL029C_p)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YOL079W(YOL079W_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL153C(YOL153C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Hypothetical protein Gene:YOR114W(YOR114W_p)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL260W(YPL260W_p)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR114W(YPR114W_p)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function Gene:YPR197C(YPR197C_d)|FD-Score:5.38|P-value:3.75E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL142W5.062.15E-70.82CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YOL021C4.231.16E-50.55DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YBR256C3.691.14E-40.39RIB5Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
YNL110C3.294.94E-40.04NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YER022W3.265.63E-40.03SRB4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
YEL019C3.236.15E-40.05MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YJR064W3.187.42E-40.09CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJL015C_d3.090.001010.14YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YPR133C2.950.001590.07SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YGR264C2.880.001980.04MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YNR011C2.840.002280.08PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YMR309C2.760.002930.04NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YKR025W2.720.003280.07RPC37RNA polymerase III subunit C37
YKL122C2.650.004050.00SRP21Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
YJR067C2.650.004070.03YAE1Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL031C5.852.53E-9RPL24ARibosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication
YPR197C_d5.383.75E-8YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR114W_p5.191.07E-7YOR114W_pPutative protein of unknown function; null mutant is viable
YOL145C4.807.97E-7CTR9Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats
YGL054C4.661.61E-6ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YJR105W4.582.29E-6ADO1Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
YCR098C4.513.20E-6GIT1Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability
YMR205C4.434.74E-6PFK2Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
YKR073C_d4.327.86E-6YKR073C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOR147W4.151.64E-5MDM32Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
YLR065C4.141.73E-5ENV10Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
YOL029C_p4.082.26E-5YOL029C_pPutative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens
YCR034W4.032.84E-5FEN1Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication
YHR098C3.963.74E-5SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YDR024W_d3.944.16E-5FYV1_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin

GO enrichment analysis for SGTC_25
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0899.33E-12SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.185185
0.0881.85E-11SGTC_450kpi-0036 14.9 μMChemDiv (Drug-like library)27950210.125excess fatty acid
0.0791.33E-9SGTC_270485-0001 32.6 μMChemDiv (Drug-like library)20544790.160714RPP1 & pyrimidine depletion
0.0782.34E-9SGTC_260302-0163 425.2 μMChemDiv (Drug-like library)31009150.114754heme biosynthesis & mitochondrial translocase
0.0756.90E-9SGTC_15324',5'-dihydroxyflavone 78.7 μMTimTec (Pure natural product library)1655210.155172Golgi
0.0732.19E-8SGTC_9831491-0821 12.2 μMChemDiv (Drug-like library)464951130.0434783
0.0723.02E-8SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.056338
0.0715.78E-8SGTC_475α-linolenic acid 10.0 μMICCB bioactive library52809340.0333333excess fatty acid
0.0708.02E-8SGTC_1075aripiprazole 15.2 μMMiscellaneous607950.0465116
0.0691.22E-7SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.0555556exosome
0.0691.32E-7SGTC_2731amoxapine 43.4 μMMiscellaneous21700.0857143
0.0663.43E-7SGTC_6281636-0253 16.0 μMChemDiv (Drug-like library)44561430.116667sphingolipid biosynthesis & PDR1
0.0663.90E-7SGTC_7820083-0095 30.5 μMChemDiv (Drug-like library)35917290.118421
0.0654.78E-7SGTC_1040074-0074 591.0 μMChemDiv (Drug-like library)39431240.123077
0.0655.61E-7SGTC_3071501-1223 22.3 μMChemDiv (Drug-like library)28303290.0571429

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8690564-008044.1 μM0.326843321ChemDiv (Drug-like library)317.34133.93125
SGTC_12140358-003163.7 μM0.3090914301366ChemDiv (Drug-like library)254.31032.50214
SGTC_13802291-0730164 μM0.32999191ChemDiv (Drug-like library)334.394964.25814
SGTC_1169aniline yellow25.8 μM0.2926836051ChemDiv (Drug-like library)197.235843.45213
SGTC_8550428-002025 μM0.2857146812414ChemDiv (Drug-like library)226.23072.15914Golgi
SGTC_5761073-011322.7 μM0.2571435439563ChemDiv (Drug-like library)367.832123.87114redox potentiating
SGTC_12330325-040814.2 μM0.25581412874ChemDiv (Drug-like library)211.262423.93813
SGTC_2869903785832.47 μM0.23809527187042Chembridge (Drug-like library)326.78023.21113
SGTC_3075911626949.47 μM0.23728817015205Chembridge (Drug-like library)293.280142.78206
SGTC_6911548-0686185 μM0.23684246495133ChemDiv (Drug-like library)485.920162.66227