5-alpha-androstan-3,17-dione

(5S,10S,13S)-10,13-dimethyl-2,4,5,6,7,8,9,11,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthrene-3,17-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2500
Screen concentration 61.4 μM
Source Microsource (Natural product library)
PubChem CID 6710655
SMILES CC12CCC(=O)CC1CCC3C2CCC4(C3CCC4=O)C
Standardized SMILES CC12CCC(=O)CC1CCC3C2CCC4(C)C3CCC4=O
Molecular weight 288.4244
ALogP 3.4
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.16
% growth inhibition (Hom. pool) 7.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6710655
Download HIP data (tab-delimited text)  (excel)
Gene:ARP3(YJR065C)|FD-Score:3.85|P-value:5.80E-5|Clearance:0.06||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CAK1(YFL029C)|FD-Score:3.32|P-value:4.54E-4|Clearance:0.02||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CWC2(YDL209C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.11||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:ERG25(YGR060W)|FD-Score:3.86|P-value:5.70E-5|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:GCD11(YER025W)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:MAS1(YLR163C)|FD-Score:-4.02|P-value:2.93E-5|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NEO1(YIL048W)|FD-Score:4.12|P-value:1.93E-5|Clearance:0.26||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NUP159(YIL115C)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.1||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:ORC4(YPR162C)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.02||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:POL2(YNL262W)|FD-Score:3.79|P-value:7.52E-5|Clearance:0.16||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:RAP1(YNL216W)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.05||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RFA1(YAR007C)|FD-Score:3.63|P-value:1.44E-4|Clearance:0.03||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:ROT1(YMR200W)|FD-Score:3.3|P-value:4.84E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPL30(YGL030W)|FD-Score:-3.46|P-value:2.74E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RSC6(YCR052W)|FD-Score:-3.36|P-value:3.93E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC53(YFL045C)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.11||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:TAF12(YDR145W)|FD-Score:3.25|P-value:5.76E-4|Clearance:0.05||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YBL077W(YBL077W_d)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YGR073C(YGR073C_d)|FD-Score:3.39|P-value:3.49E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:ARP3(YJR065C)|FD-Score:3.85|P-value:5.80E-5|Clearance:0.06||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CAK1(YFL029C)|FD-Score:3.32|P-value:4.54E-4|Clearance:0.02||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CWC2(YDL209C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.11||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:ERG25(YGR060W)|FD-Score:3.86|P-value:5.70E-5|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:GCD11(YER025W)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:MAS1(YLR163C)|FD-Score:-4.02|P-value:2.93E-5|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NEO1(YIL048W)|FD-Score:4.12|P-value:1.93E-5|Clearance:0.26||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NUP159(YIL115C)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.1||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:ORC4(YPR162C)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.02||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:POL2(YNL262W)|FD-Score:3.79|P-value:7.52E-5|Clearance:0.16||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:RAP1(YNL216W)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.05||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RFA1(YAR007C)|FD-Score:3.63|P-value:1.44E-4|Clearance:0.03||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:ROT1(YMR200W)|FD-Score:3.3|P-value:4.84E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPL30(YGL030W)|FD-Score:-3.46|P-value:2.74E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RSC6(YCR052W)|FD-Score:-3.36|P-value:3.93E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC53(YFL045C)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.11||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:TAF12(YDR145W)|FD-Score:3.25|P-value:5.76E-4|Clearance:0.05||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YBL077W(YBL077W_d)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YGR073C(YGR073C_d)|FD-Score:3.39|P-value:3.49E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6710655
Download HOP data (tab-delimited text)  (excel)
Gene:AAD4(YDL243C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADA2(YDR448W)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADE5,7(YGL234W)|FD-Score:-3.84|P-value:6.10E-5||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:ADY4(YLR227C)|FD-Score:-4.34|P-value:7.00E-6||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:AIM4(YBR194W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ARO1(YDR127W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:9.18|P-value:2.26E-20||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO9(YHR137W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATP25(YMR098C)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:ATP4(YPL078C)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:AVT5(YBL089W)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:AZF1(YOR113W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BNI1(YNL271C)|FD-Score:4.86|P-value:5.83E-7||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRO1(YPL084W)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CCE1(YKL011C)|FD-Score:-5.11|P-value:1.60E-7||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:COX9(YDL067C)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUS2(YNL286W)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DAK2(YFL053W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DAL7(YIR031C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DIE2(YGR227W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ECM32(YER176W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ERG3(YLR056W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERV25(YML012W)|FD-Score:4|P-value:3.16E-5||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FAA4(YMR246W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FTR1(YER145C)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GCG1(YER163C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GCN2(YDR283C)|FD-Score:6.56|P-value:2.66E-11||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:7.27|P-value:1.85E-13||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:6.35|P-value:1.07E-10||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HTA1(YDR225W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IMG1(YCR046C)|FD-Score:-4.35|P-value:6.82E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:LAP3(YNL239W)|FD-Score:5.81|P-value:3.14E-9||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LDB16(YCL005W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LEU9(YOR108W)|FD-Score:4.84|P-value:6.37E-7||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MDM20(YOL076W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MDM32(YOR147W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MHR1(YDR296W)|FD-Score:-5.48|P-value:2.15E-8||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MSH2(YOL090W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSS18(YPR134W)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:OPI11(YPR044C_d)|FD-Score:-3.59|P-value:1.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PAC10(YGR078C)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PCI8(YIL071C)|FD-Score:5.21|P-value:9.57E-8||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCL8(YPL219W)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PET122(YER153C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PHO88(YBR106W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIH1(YHR034C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMP3(YDR276C)|FD-Score:6.12|P-value:4.72E-10||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PMT2(YAL023C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PMT7(YDR307W_p)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:RBK1(YCR036W)|FD-Score:4.92|P-value:4.37E-7||SGD DESC:Putative ribokinase Gene:REG1(YDR028C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGD1(YBR260C)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RPH1(YER169W)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPI1(YIL119C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPN4(YDL020C)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT12(YCR045C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSC1(YGR056W)|FD-Score:6|P-value:1.00E-9||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSM22(YKL155C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RSM7(YJR113C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAP155(YFR040W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SAP30(YMR263W)|FD-Score:5.52|P-value:1.71E-8||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SET2(YJL168C)|FD-Score:-6.25|P-value:2.03E-10||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SET5(YHR207C)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SLT2(YHR030C)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMK1(YPR054W)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SNF5(YBR289W)|FD-Score:-3.77|P-value:8.18E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SSD1(YDR293C)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSK2(YNR031C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SVL3(YPL032C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TDH2(YJR009C)|FD-Score:-6.16|P-value:3.71E-10||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:-5.43|P-value:2.78E-8||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:6.1|P-value:5.31E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.04|P-value:2.29E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.77|P-value:6.42E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.72|P-value:9.89E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBP13(YBL067C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VAN1(YML115C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VMA3(YEL027W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS1(YKR001C)|FD-Score:-4.29|P-value:8.89E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS21(YOR089C)|FD-Score:6.62|P-value:1.79E-11||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:-5.22|P-value:8.92E-8||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR174C(YBR174C_d)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR259W(YBR259W_p)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCH1(YGR203W)|FD-Score:5.23|P-value:8.40E-8||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR008C(YDR008C_d)|FD-Score:4.98|P-value:3.17E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YGL117W(YGL117W_p)|FD-Score:6.74|P-value:7.88E-12||SGD DESC:Putative protein of unknown function Gene:YGL188C-A(YGL188C-A_p)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Putative protein of unknown function Gene:YGR291C(YGR291C_d)|FD-Score:5.32|P-value:5.13E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL025C(YIL025C_d)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR042C(YIR042C_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YLR001C(YLR001C_p)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YML012C-A(YML012C-A_d)|FD-Score:5.76|P-value:4.26E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR1(YJR110W)|FD-Score:-3.76|P-value:8.52E-5||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YMR221C(YMR221C_p)|FD-Score:7.67|P-value:8.76E-15||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YNL195C(YNL195C_p)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL203C(YNL203C_d)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL106W(YOL106W_d)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR338W(YOR338W_p)|FD-Score:4.39|P-value:5.80E-6||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:9.07|P-value:5.91E-20||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:AAD4(YDL243C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADA2(YDR448W)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADE5,7(YGL234W)|FD-Score:-3.84|P-value:6.10E-5||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:ADY4(YLR227C)|FD-Score:-4.34|P-value:7.00E-6||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:AIM4(YBR194W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ARO1(YDR127W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:9.18|P-value:2.26E-20||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO9(YHR137W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATP25(YMR098C)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:ATP4(YPL078C)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:AVT5(YBL089W)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:AZF1(YOR113W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BNI1(YNL271C)|FD-Score:4.86|P-value:5.83E-7||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRO1(YPL084W)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CCE1(YKL011C)|FD-Score:-5.11|P-value:1.60E-7||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:COX9(YDL067C)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUS2(YNL286W)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DAK2(YFL053W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DAL7(YIR031C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DIE2(YGR227W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ECM32(YER176W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ERG3(YLR056W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERV25(YML012W)|FD-Score:4|P-value:3.16E-5||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FAA4(YMR246W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FTR1(YER145C)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GCG1(YER163C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GCN2(YDR283C)|FD-Score:6.56|P-value:2.66E-11||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:7.27|P-value:1.85E-13||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:6.35|P-value:1.07E-10||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HTA1(YDR225W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IMG1(YCR046C)|FD-Score:-4.35|P-value:6.82E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:LAP3(YNL239W)|FD-Score:5.81|P-value:3.14E-9||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LDB16(YCL005W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LEU9(YOR108W)|FD-Score:4.84|P-value:6.37E-7||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MDM20(YOL076W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MDM32(YOR147W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MHR1(YDR296W)|FD-Score:-5.48|P-value:2.15E-8||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MSH2(YOL090W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSS18(YPR134W)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:OPI11(YPR044C_d)|FD-Score:-3.59|P-value:1.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PAC10(YGR078C)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PCI8(YIL071C)|FD-Score:5.21|P-value:9.57E-8||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCL8(YPL219W)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PET122(YER153C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PHO88(YBR106W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIH1(YHR034C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMP3(YDR276C)|FD-Score:6.12|P-value:4.72E-10||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PMT2(YAL023C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PMT7(YDR307W_p)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:RBK1(YCR036W)|FD-Score:4.92|P-value:4.37E-7||SGD DESC:Putative ribokinase Gene:REG1(YDR028C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGD1(YBR260C)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RPH1(YER169W)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPI1(YIL119C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPN4(YDL020C)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT12(YCR045C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSC1(YGR056W)|FD-Score:6|P-value:1.00E-9||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSM22(YKL155C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RSM7(YJR113C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAP155(YFR040W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SAP30(YMR263W)|FD-Score:5.52|P-value:1.71E-8||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SET2(YJL168C)|FD-Score:-6.25|P-value:2.03E-10||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SET5(YHR207C)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SLT2(YHR030C)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMK1(YPR054W)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SNF5(YBR289W)|FD-Score:-3.77|P-value:8.18E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SSD1(YDR293C)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSK2(YNR031C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SVL3(YPL032C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TDH2(YJR009C)|FD-Score:-6.16|P-value:3.71E-10||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:-5.43|P-value:2.78E-8||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:6.1|P-value:5.31E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.04|P-value:2.29E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.77|P-value:6.42E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.72|P-value:9.89E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBP13(YBL067C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VAN1(YML115C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VMA3(YEL027W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS1(YKR001C)|FD-Score:-4.29|P-value:8.89E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS21(YOR089C)|FD-Score:6.62|P-value:1.79E-11||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:-5.22|P-value:8.92E-8||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR174C(YBR174C_d)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR259W(YBR259W_p)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCH1(YGR203W)|FD-Score:5.23|P-value:8.40E-8||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR008C(YDR008C_d)|FD-Score:4.98|P-value:3.17E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YGL117W(YGL117W_p)|FD-Score:6.74|P-value:7.88E-12||SGD DESC:Putative protein of unknown function Gene:YGL188C-A(YGL188C-A_p)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Putative protein of unknown function Gene:YGR291C(YGR291C_d)|FD-Score:5.32|P-value:5.13E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL025C(YIL025C_d)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR042C(YIR042C_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YLR001C(YLR001C_p)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YML012C-A(YML012C-A_d)|FD-Score:5.76|P-value:4.26E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR1(YJR110W)|FD-Score:-3.76|P-value:8.52E-5||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YMR221C(YMR221C_p)|FD-Score:7.67|P-value:8.76E-15||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YNL195C(YNL195C_p)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL203C(YNL203C_d)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL106W(YOL106W_d)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR338W(YOR338W_p)|FD-Score:4.39|P-value:5.80E-6||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:9.07|P-value:5.91E-20||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W4.121.93E-50.26NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YGR060W3.865.70E-50.00ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YJR065C3.855.80E-50.06ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YNL262W3.797.52E-50.17POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
YAR007C3.631.44E-40.03RFA1Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
YIL115C3.591.64E-40.10NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YFL045C3.502.36E-40.11SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YGR073C_d3.393.49E-40.03YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YBL077W_d3.363.84E-40.05YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YFL029C3.324.54E-40.02CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YMR200W3.304.84E-44.04E-4ROT1Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
YNL216W3.304.85E-40.05RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YDR145W3.255.76E-40.04TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YPR162C3.216.74E-40.02ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YDL209C3.197.16E-40.11CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR035W9.182.26E-20ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YNL237W9.075.91E-20YTP1Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
YMR221C_p7.678.76E-15YMR221C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy
YKR026C7.271.85E-13GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YDR354W6.776.42E-12TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YGL117W_p6.747.88E-12YGL117W_pPutative protein of unknown function
YOR089C6.621.79E-11VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YDR283C6.562.66E-11GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YEL009C6.351.07E-10GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR276C6.124.72E-10PMP3Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential
YDR007W6.105.31E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YGR056W6.001.00E-9RSC1Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
YNL239W5.813.14E-9LAP3Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
YML012C-A_d5.764.26E-9YML012C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
YMR263W5.521.71E-8SAP30Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance

GO enrichment analysis for SGTC_2500
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3443.12E-164SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.075
0.3444.88E-163SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.45098
0.3181.33E-138SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.45098
0.3082.10E-130SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0263158
0.3064.47E-128SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0597015
0.3002.64E-123SGTC_14234048-0112 5.6 μMChemDiv (Drug-like library)X14230.0470588
0.2978.04E-121SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.037037
0.2871.56E-112SGTC_18825615643 20.0 μMMiscellaneous22530750.0487805TRP & mitochondrial translation
0.2861.13E-111SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0615385
0.2799.18E-106SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.644444
0.2741.72E-102SGTC_14274048-4900 550.0 nMChemDiv (Drug-like library)241325860.0645161
0.2732.64E-101SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.141026
0.2691.56E-98SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)6710748NA
0.2663.37E-96SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0405405
0.2664.41E-96SGTC_24885268135 45.7 μMMiscellaneous12703560.0461538

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1572epiandrosterone68.9 μM0.644444441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.644444441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.6444442754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_2650prasterone100 μM0.4509816759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.450985881Miscellaneous288.424423.33812
SGTC_1974st06944826.9 μM0.4230776323587TimTec (Natural product derivative library)305.454943.36923
SGTC_406estrone100 μM0.3755870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.375698TimTec (Pure natural product library)270.366083.93812DNA intercalators
SGTC_1750st0818592.36 μM0.345291695TimTec (Natural product derivative library)275.472024.43511endomembrane recycling
SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one66.67 μM0.3333336708561Microsource (Natural product library)228.32943.65701
SGTC_1586deoxycorticosterone acetate53.7 μM0.2835825952TimTec (Pure natural product library)372.497783.56704