purpurogallin

2,3,4,5-tetrahydroxybenzo[7]annulen-6-one

An antioxidant.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Anti-Infective Agents;Antioxidants;Enzyme Inhibitors;Food Preservatives;Serine Proteinase Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2504
Screen concentration 52.3 μM
Source Microsource (Natural product library)
PubChem CID 5281571
SMILES C1=CC(=O)C(=C2C(=C1)C=C(C(=C2O)O)O)O
Standardized SMILES OC1=CC=Cc2cc(O)c(O)c(O)c2C1=O
Molecular weight 220.1782
ALogP -0.33
H-bond donor count 4
H-bond acceptor count 5
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.73
% growth inhibition (Hom. pool) 6.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5281571
Download HIP data (tab-delimited text)  (excel)
Gene:CDC11(YJR076C)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.07||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:GCD6(YDR211W)|FD-Score:5.63|P-value:8.84E-9|Clearance:1.46||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:MRPS18(YNL306W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.1||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NIC96(YFR002W)|FD-Score:-3.75|P-value:8.97E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP56(YLR197W)|FD-Score:-3.74|P-value:9.14E-5|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:POL30(YBR088C)|FD-Score:-3.6|P-value:1.61E-4|Clearance:0||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:PRE5(YMR314W)|FD-Score:3.76|P-value:8.56E-5|Clearance:0.11||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:SRP1(YNL189W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.33||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:SWD2(YKL018W)|FD-Score:3.13|P-value:8.64E-4|Clearance:0.05||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TEN1(YLR010C)|FD-Score:4.11|P-value:2.01E-5|Clearance:0.35||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:VAS1(YGR094W)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.08||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:CDC11(YJR076C)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.07||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:GCD6(YDR211W)|FD-Score:5.63|P-value:8.84E-9|Clearance:1.46||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:MRPS18(YNL306W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.1||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NIC96(YFR002W)|FD-Score:-3.75|P-value:8.97E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP56(YLR197W)|FD-Score:-3.74|P-value:9.14E-5|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:POL30(YBR088C)|FD-Score:-3.6|P-value:1.61E-4|Clearance:0||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:PRE5(YMR314W)|FD-Score:3.76|P-value:8.56E-5|Clearance:0.11||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:SRP1(YNL189W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.33||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:SWD2(YKL018W)|FD-Score:3.13|P-value:8.64E-4|Clearance:0.05||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TEN1(YLR010C)|FD-Score:4.11|P-value:2.01E-5|Clearance:0.35||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:VAS1(YGR094W)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.08||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5281571
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ACO1(YLR304C)|FD-Score:6.57|P-value:2.47E-11||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AEP1(YMR064W)|FD-Score:-4.44|P-value:4.42E-6||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:APD1(YBR151W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARG82(YDR173C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO3(YDR035W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATX1(YNL259C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BSC1(YDL037C)|FD-Score:-3.12|P-value:9.15E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BUD21(YOR078W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:COQ5(YML110C)|FD-Score:-4.62|P-value:1.93E-6||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CWH41(YGL027C)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:CYB2(YML054C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DCG1(YIR030C)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DUS1(YML080W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 Gene:ECM31(YBR176W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ELP3(YPL086C)|FD-Score:-4.32|P-value:7.64E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ENB1(YOL158C)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:ERV14(YGL054C)|FD-Score:4.32|P-value:7.76E-6||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FBP1(YLR377C)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FIT2(YOR382W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMP48(YGR052W_p)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FMP52(YER004W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FTR1(YER145C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FUI1(YBL042C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GEF1(YJR040W)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:HIS6(YIL020C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HMI1(YOL095C)|FD-Score:-4.02|P-value:2.91E-5||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HOM3(YER052C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:ICY2(YPL250C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:ITC1(YGL133W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KHA1(YJL094C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:KRE1(YNL322C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:MDM34(YGL219C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MET13(YGL125W)|FD-Score:-3.8|P-value:7.38E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:OMS1(YDR316W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PMR1(YGL167C)|FD-Score:5.39|P-value:3.52E-8||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PRC1(YMR297W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRK1(YIL095W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUF2(YPR042C)|FD-Score:5.22|P-value:8.73E-8||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RAV1(YJR033C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RDR1(YOR380W)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:REG1(YDR028C)|FD-Score:-4.49|P-value:3.54E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFM1(YOR279C)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RHB1(YCR027C)|FD-Score:4.84|P-value:6.55E-7||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPL16B(YNL069C)|FD-Score:-4.34|P-value:7.10E-6||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL22B(YFL034C-A)|FD-Score:-5.16|P-value:1.25E-7||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTT102(YGR275W)|FD-Score:-3.73|P-value:9.45E-5||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SCS7(YMR272C)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SIP5(YMR140W)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKG3(YLR187W_p)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SKI2(YLR398C)|FD-Score:-4.39|P-value:5.78E-6||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SPO7(YAL009W)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPS100(YHR139C)|FD-Score:-3.09|P-value:0.00100||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SSQ1(YLR369W)|FD-Score:4.83|P-value:6.70E-7||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:STF2(YGR008C)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:SWH1(YAR042W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:TPM2(YIL138C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRM7(YBR061C)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP5(YGL026C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSA1(YML028W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:UBR1(YGR184C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:ULI1(YFR026C)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:UPS3(YDR185C)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VMA1(YDL185W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA16(YHR026W)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS1(YKR001C)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR064W(YBR064W_d)|FD-Score:-4.99|P-value:3.03E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR226C(YBR226C_d)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YDR194W-A(YDR194W-A_p)|FD-Score:6.25|P-value:2.11E-10||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR455C(YDR455C_d)|FD-Score:5.69|P-value:6.22E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER053C-A(YER053C-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YGL010W(YGL010W_p)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL217C(YGL217C_d)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YIL001W(YIL001W_p)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL028W(YJL028W)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YKL118W(YKL118W_d)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR269C(YLR269C_d)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR334C(YLR334C_d)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YOL029C(YOL029C_p)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YPL225W(YPL225W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:AAT2(YLR027C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ACO1(YLR304C)|FD-Score:6.57|P-value:2.47E-11||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AEP1(YMR064W)|FD-Score:-4.44|P-value:4.42E-6||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:APD1(YBR151W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARG82(YDR173C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO3(YDR035W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATX1(YNL259C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BSC1(YDL037C)|FD-Score:-3.12|P-value:9.15E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BUD21(YOR078W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:COQ5(YML110C)|FD-Score:-4.62|P-value:1.93E-6||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CWH41(YGL027C)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:CYB2(YML054C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DCG1(YIR030C)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DUS1(YML080W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 Gene:ECM31(YBR176W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ELP3(YPL086C)|FD-Score:-4.32|P-value:7.64E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ENB1(YOL158C)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:ERV14(YGL054C)|FD-Score:4.32|P-value:7.76E-6||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FBP1(YLR377C)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FIT2(YOR382W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMP48(YGR052W_p)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FMP52(YER004W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FTR1(YER145C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FUI1(YBL042C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GEF1(YJR040W)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:HIS6(YIL020C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HMI1(YOL095C)|FD-Score:-4.02|P-value:2.91E-5||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HOM3(YER052C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:ICY2(YPL250C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:ITC1(YGL133W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KHA1(YJL094C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:KRE1(YNL322C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:MDM34(YGL219C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MET13(YGL125W)|FD-Score:-3.8|P-value:7.38E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:OMS1(YDR316W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PMR1(YGL167C)|FD-Score:5.39|P-value:3.52E-8||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PRC1(YMR297W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRK1(YIL095W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUF2(YPR042C)|FD-Score:5.22|P-value:8.73E-8||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RAV1(YJR033C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RDR1(YOR380W)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:REG1(YDR028C)|FD-Score:-4.49|P-value:3.54E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFM1(YOR279C)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RHB1(YCR027C)|FD-Score:4.84|P-value:6.55E-7||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPL16B(YNL069C)|FD-Score:-4.34|P-value:7.10E-6||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL22B(YFL034C-A)|FD-Score:-5.16|P-value:1.25E-7||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTT102(YGR275W)|FD-Score:-3.73|P-value:9.45E-5||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SCS7(YMR272C)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SIP5(YMR140W)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKG3(YLR187W_p)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SKI2(YLR398C)|FD-Score:-4.39|P-value:5.78E-6||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SPO7(YAL009W)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPS100(YHR139C)|FD-Score:-3.09|P-value:0.00100||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SSQ1(YLR369W)|FD-Score:4.83|P-value:6.70E-7||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:STF2(YGR008C)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:SWH1(YAR042W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:TPM2(YIL138C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRM7(YBR061C)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP5(YGL026C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSA1(YML028W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:UBR1(YGR184C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:ULI1(YFR026C)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:UPS3(YDR185C)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VMA1(YDL185W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA16(YHR026W)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS1(YKR001C)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR064W(YBR064W_d)|FD-Score:-4.99|P-value:3.03E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR226C(YBR226C_d)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YDR194W-A(YDR194W-A_p)|FD-Score:6.25|P-value:2.11E-10||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR455C(YDR455C_d)|FD-Score:5.69|P-value:6.22E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER053C-A(YER053C-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YGL010W(YGL010W_p)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL217C(YGL217C_d)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YIL001W(YIL001W_p)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL028W(YJL028W)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YKL118W(YKL118W_d)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR269C(YLR269C_d)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR334C(YLR334C_d)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YOL029C(YOL029C_p)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YPL225W(YPL225W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YDR211W5.638.84E-91.46GCD6Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
YJR076C4.181.48E-50.07CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YLR010C4.112.01E-50.35TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YMR314W3.768.56E-50.11PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YNL306W3.651.33E-40.10MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
YNL189W3.541.97E-40.33SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YGR094W3.226.49E-40.08VAS1Mitochondrial and cytoplasmic valyl-tRNA synthetase
YKL018W3.138.64E-40.05SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
YMR197C3.080.001020.13VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YML043C2.950.001590.01RRN11Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YIL091C2.940.001660.02UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YGR172C2.920.001760.13YIP1Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
YLR276C2.790.002640.02DBP9DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
YMR013C2.770.002790.02SEC59Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
YDR404C2.750.002940.03RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR304C6.572.47E-11ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YDR194W-A_p6.252.11E-10YDR194W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YDR455C_d5.696.22E-9YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YGL167C5.393.52E-8PMR1High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
YPR042C5.228.73E-8PUF2Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
YOL029C_p5.181.09E-7YOL029C_pPutative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens
YOR380W4.875.67E-7RDR1Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins
YCR027C4.846.55E-7RHB1Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins
YLR369W4.836.70E-7SSQ1Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia
YIR030C4.651.68E-6DCG1Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain
YOR078W4.651.69E-6BUD21Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
YAL009W4.641.72E-6SPO7Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
YJR040W4.602.14E-6GEF1Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
YGR052W_p4.347.19E-6FMP48_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
YGL054C4.327.76E-6ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2504
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1231.78E-21SGTC_2615purpurogallin-4-carboxylic acid 100.0 μMMicrosource (Natural product library)2693150.487179redox potentiating
0.1071.67E-16SGTC_263alizarin 107.9 μMMiscellaneous62930.243902iron homeostasis
0.1001.66E-14SGTC_2505galangin 7.9 μMMicrosource (Natural product library)52816160.2RPP1 & pyrimidine depletion
0.0976.90E-14SGTC_22757947463 200.0 μMChembridge (Fragment library)12254140.0677966iron homeostasis
0.0961.50E-13SGTC_23899091542 195.5 μMChembridge (Fragment library)36266240.0526316iron homeostasis
0.0903.42E-12SGTC_20464110841 106.0 μMChembridge (Fragment library)441196300.0638298iron homeostasis
0.0904.57E-12SGTC_2666tannic acid 100.0 μMMicrosource (Natural product library)161342670.140625iron homeostasis
0.0849.51E-11SGTC_2675theaflavin digallate 100.0 μMMicrosource (Natural product library)4673200.194444iron homeostasis
0.0849.68E-11SGTC_26092,3,4-trihydroxy-4'-ethoxybenzophenone 70.0 μMMicrosource (Natural product library)67087390.153846redox potentiating
0.0832.01E-10SGTC_2586curcumin 80.0 μMICCB bioactive library66103320.109375iron homeostasis
0.0808.11E-10SGTC_2513baicalein 25.6 μMMicrosource (Natural product library)52816050.276596calcium & mitochondrial duress
0.0781.64E-9SGTC_1571348-1695 65.8 μMChemDiv (Drug-like library)53805100.107143iron homeostasis
0.0782.44E-9SGTC_22917917025 104.3 μMChembridge (Fragment library)29646120.0625iron homeostasis
0.0757.83E-9SGTC_2597laccaic acid a 100.0 μMMicrosource (Natural product library)54914150.184615
0.0741.16E-8SGTC_6970508-1955 564.0 μMChemDiv (Drug-like library)62431130.0434783

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2682pyrogallin40.03 μM0.636364822798Microsource (Natural product library)204.178820.2134copper-dependent oxidative stress
SGTC_2615purpurogallin-4-carboxylic acid100 μM0.487179269315Microsource (Natural product library)264.18772-0.73757redox potentiating
SGTC_2507purpurin79.67 μM0.3076926683Microsource (Natural product library)256.210322.08235copper-dependent oxidative stress
SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate54.59 μM0.2916675098884Microsource (Natural product library)262.21490.02636RPP1 & pyrimidine depletion
SGTC_2513baicalein25.65 μM0.2765965281605Microsource (Natural product library)270.23692.4135calcium & mitochondrial duress
SGTC_263alizarin107.93 μM0.2439026293Miscellaneous240.210922.32424iron homeostasis
SGTC_2686alizarin95.37 μM0.2439026293Miscellaneous240.210922.32424RPP1 & pyrimidine depletion
SGTC_2671danthron100 μM0.2368422950Microsource (Natural product library)240.210922.32424DNA damage response
SGTC_2041510744245.34 μM0.232558793005Chembridge (Fragment library)188.179421.82913RSC complex & mRNA processing
SGTC_1114scopoletin1.73 μM0.2127665280460TimTec (Natural product library)192.168121.64114
SGTC_1530rhein70.4 μM0.20408210168TimTec (Pure natural product library)284.220421.95436