celastrol

(2R,4aS,6aR,6aS,14aS,14bR)-10-hydroxy-2,4a,6a,6a,9,14a-hexamethyl-11-oxo-1,3,4,5,6,13,14,14b-octahydropicene-2-carboxylic acid

Celastrol is a natural product and antioxidant.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2508
Screen concentration 40.5 μM
Source Microsource (Natural product library)
PubChem CID 122724
SMILES CC1=C(C(=O)C=C2C1=CC=C3C2(CCC4(C3(CCC5(C4CC(CC5)(C)C(=O)O)C)C)C)C)O
Standardized SMILES CC1=C(O)C(=O)C=C2C1=CC=C3C2(C)CCC4(C)C5CC(C)(CCC5(C)CCC34C)C(=O)O
Molecular weight 450.6096
ALogP 5.48
H-bond donor count 2
H-bond acceptor count 4
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.57
% growth inhibition (Hom. pool) 8.52


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 122724
Download HIP data (tab-delimited text)  (excel)
Gene:CCT4(YDL143W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.19||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC1(YDR182W)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:GCR1(YPL075W)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:GPI17(YDR434W)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:HTB1(YDR224C)|FD-Score:-3.36|P-value:3.89E-4|Clearance:0||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:LSM5(YER146W)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.33||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MTR4(YJL050W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NOP19(YGR251W)|FD-Score:-3.75|P-value:8.93E-5|Clearance:0||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:PBN1(YCL052C)|FD-Score:3.38|P-value:3.62E-4|Clearance:0.09||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PKC1(YBL105C)|FD-Score:7.83|P-value:2.48E-15|Clearance:2.61||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPP1(YHR062C)|FD-Score:5.22|P-value:8.96E-8|Clearance:0.27||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC14(YMR079W)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.44||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SHR3(YDL212W)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SPN1(YPR133C)|FD-Score:3.29|P-value:5.06E-4|Clearance:0.18||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TEM1(YML064C)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:TOM40(YMR203W)|FD-Score:4.95|P-value:3.80E-7|Clearance:0.93||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TUB2(YFL037W)|FD-Score:-5.59|P-value:1.13E-8|Clearance:0||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR020W(YHR020W)|FD-Score:-3.22|P-value:6.33E-4|Clearance:0||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:YPR136C(YPR136C_d)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:CCT4(YDL143W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.19||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC1(YDR182W)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:GCR1(YPL075W)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:GPI17(YDR434W)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:HTB1(YDR224C)|FD-Score:-3.36|P-value:3.89E-4|Clearance:0||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:LSM5(YER146W)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.33||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MTR4(YJL050W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NOP19(YGR251W)|FD-Score:-3.75|P-value:8.93E-5|Clearance:0||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:PBN1(YCL052C)|FD-Score:3.38|P-value:3.62E-4|Clearance:0.09||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PKC1(YBL105C)|FD-Score:7.83|P-value:2.48E-15|Clearance:2.61||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPP1(YHR062C)|FD-Score:5.22|P-value:8.96E-8|Clearance:0.27||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC14(YMR079W)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.44||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SHR3(YDL212W)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SPN1(YPR133C)|FD-Score:3.29|P-value:5.06E-4|Clearance:0.18||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TEM1(YML064C)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:TOM40(YMR203W)|FD-Score:4.95|P-value:3.80E-7|Clearance:0.93||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TUB2(YFL037W)|FD-Score:-5.59|P-value:1.13E-8|Clearance:0||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR020W(YHR020W)|FD-Score:-3.22|P-value:6.33E-4|Clearance:0||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:YPR136C(YPR136C_d)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 122724
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM37(YNL100W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APM4(YOL062C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:APN2(YBL019W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII Gene:AVT1(YJR001W)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BAG7(YOR134W)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:CIN1(YOR349W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COG7(YGL005C)|FD-Score:6.75|P-value:7.15E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTM1(YHR109W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CTS2(YDR371W_p)|FD-Score:-5.06|P-value:2.08E-7||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:DPB3(YBR278W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ENV10(YLR065C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:GAL11(YOL051W)|FD-Score:5.48|P-value:2.17E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GEP4(YHR100C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GET2(YER083C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLE2(YER107C)|FD-Score:3.75|P-value:8.69E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GNP1(YDR508C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GRX3(YDR098C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:GUP1(YGL084C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HER2(YMR293C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HPT1(YDR399W)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:HTL1(YCR020W-B)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:INM2(YDR287W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:LHS1(YKL073W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MMS22(YLR320W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN11(YJL183W)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPS5(YBR251W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NPR3(YHL023C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NTO1(YPR031W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PAU4(YLR461W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PDR1(YGL013C)|FD-Score:16.9|P-value:2.13E-64||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO88(YBR106W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PRS3(YHL011C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK2(YJR059W)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAD17(YOR368W)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RCE1(YMR274C)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RNH202(YDR279W)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPL15B(YMR121C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPN4(YDL020C)|FD-Score:11.5|P-value:8.13E-31||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT6(YGL146C_p)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:SAC6(YDR129C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SPT4(YGR063C)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRC1(YML034W)|FD-Score:4.8|P-value:8.06E-7||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SUR4(YLR372W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TPN1(YGL186C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.36|P-value:9.05E-14||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.8|P-value:3.29E-9||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:5.65|P-value:8.09E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:7.16|P-value:4.05E-13||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP11(YKR098C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:URE2(YNL229C)|FD-Score:5.21|P-value:9.45E-8||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YBL062W(YBL062W_d)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEL057C(YEL057C_p)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YHR050W-A(YHR050W-A_p)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL086C(YIL086C_d)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR053C(YLR053C_p)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function Gene:YLR179C(YLR179C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus Gene:YMR135W-A(YMR135W-A_d)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YND1(YER005W)|FD-Score:-5.17|P-value:1.15E-7||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL092W(YNL092W_p)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT52(YKR014C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:ACE2(YLR131C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM37(YNL100W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APM4(YOL062C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:APN2(YBL019W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII Gene:AVT1(YJR001W)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BAG7(YOR134W)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:CIN1(YOR349W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COG7(YGL005C)|FD-Score:6.75|P-value:7.15E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTM1(YHR109W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CTS2(YDR371W_p)|FD-Score:-5.06|P-value:2.08E-7||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:DPB3(YBR278W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ENV10(YLR065C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:GAL11(YOL051W)|FD-Score:5.48|P-value:2.17E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GEP4(YHR100C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GET2(YER083C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLE2(YER107C)|FD-Score:3.75|P-value:8.69E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GNP1(YDR508C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GRX3(YDR098C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:GUP1(YGL084C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HER2(YMR293C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HPT1(YDR399W)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:HTL1(YCR020W-B)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:INM2(YDR287W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:LHS1(YKL073W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MMS22(YLR320W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN11(YJL183W)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPS5(YBR251W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NPR3(YHL023C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NTO1(YPR031W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PAU4(YLR461W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PDR1(YGL013C)|FD-Score:16.9|P-value:2.13E-64||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO88(YBR106W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PRS3(YHL011C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK2(YJR059W)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAD17(YOR368W)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RCE1(YMR274C)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RNH202(YDR279W)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPL15B(YMR121C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPN4(YDL020C)|FD-Score:11.5|P-value:8.13E-31||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT6(YGL146C_p)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:SAC6(YDR129C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SPT4(YGR063C)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRC1(YML034W)|FD-Score:4.8|P-value:8.06E-7||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SUR4(YLR372W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TPN1(YGL186C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.36|P-value:9.05E-14||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.8|P-value:3.29E-9||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:5.65|P-value:8.09E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:7.16|P-value:4.05E-13||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP11(YKR098C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:URE2(YNL229C)|FD-Score:5.21|P-value:9.45E-8||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YBL062W(YBL062W_d)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEL057C(YEL057C_p)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YHR050W-A(YHR050W-A_p)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL086C(YIL086C_d)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR053C(YLR053C_p)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function Gene:YLR179C(YLR179C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus Gene:YMR135W-A(YMR135W-A_d)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YND1(YER005W)|FD-Score:-5.17|P-value:1.15E-7||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL092W(YNL092W_p)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT52(YKR014C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL105C7.832.48E-152.61PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YHR062C5.228.96E-80.27RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR203W4.953.80E-70.93TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YMR079W4.013.02E-50.44SEC14Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
YDL143W3.571.79E-40.19CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YCL052C3.383.62E-40.09PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YPR133C3.295.06E-40.18SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YER146W3.119.51E-40.33LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YJL167W2.780.002730.03ERG20Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
YOR362C2.750.002990.02PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YLR274W2.720.003220.08MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YGL093W2.640.004140.07SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YJL202C_d2.570.005030.05YJL202C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
YNL267W2.520.005870.03PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YOL069W2.490.006440.03NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C16.902.13E-64PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YDL020C11.508.13E-31RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YKL211C7.369.05E-14TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YML124C7.164.05E-13TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YGL005C6.757.15E-12COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR354W5.803.29E-9TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YBR058C-A5.658.09E-9TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YOL051W5.482.17E-8GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YNL229C5.219.45E-8URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YGL186C5.191.08E-7TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YKL126W5.131.48E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDR008C_d4.983.13E-7YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR399W4.817.52E-7HPT1Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
YML034W4.808.06E-7SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YGL025C4.356.93E-6PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor

GO enrichment analysis for SGTC_2508
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3571.56E-176SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0571429PDR1
0.3292.17E-149SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.0425532
0.3296.97E-149SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0609756TSC3-RPN4
0.3222.34E-142SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0857143PDR1
0.2993.92E-122SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.104651
0.2961.68E-119SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.045977PDR1
0.2931.66E-117SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.091954PDR1
0.2831.48E-109SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0972222PDR1
0.2811.02E-107SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0595238
0.2781.87E-105SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.1TSC3-RPN4
0.2779.82E-105SGTC_8600443-0016 25.1 μMChemDiv (Drug-like library)53379420.0897436
0.2772.69E-104SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.0729167PDR1
0.2749.24E-102SGTC_2627acetyl isogambogic acid 100.0 μMMicrosource (Natural product library)68577890.123967TSC3-RPN4
0.2717.43E-100SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0253165
0.2689.35E-98SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0697674

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1108betulinic acid729.14 nM0.21590964945TimTec (Natural product library)456.700326.49223
SGTC_1557sumaresinolic acid42.3 μM0.2159099982268TimTec (Pure natural product library)472.699725.27734
SGTC_1127asiatic acid681.4 nM0.215054470603TimTec (Natural product library)488.699124.43545
SGTC_2583asiatic acid100 μM0.2150545320556Microsource (Natural product library)488.699124.43545
SGTC_1654st01243641.8 μM0.2073174323132TimTec (Natural product derivative library)372.540843.80613
SGTC_1630st00520931.3 μM0.2056073367314TimTec (Natural product derivative library)639.904746.46507
SGTC_2664lyso-paf C-1673.48 μM0.26710742Microsource (Natural product library)504.698523.26556
SGTC_2616abietic acid100 μM0.1927716708678Microsource (Natural product library)302.4515.09312
SGTC_1260417-1624672.48 μM0.18918941912ChemDiv (Drug-like library)270.366084.07512DNA intercalators
SGTC_1580hydrocortisone 21-hemisuccinate43.2 μM0.17346916623TimTec (Pure natural product library)462.532661.50738
SGTC_26323-hydroxy-4-(succin-2-yl)-caryolane delta-lactone100 μM0.172846857784Microsource (Natural product library)320.423223.15114