berberine

Berberine is a quaternary ammonium salt and isoquinoline alkaloid.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2511
Screen concentration 10.5 μM
Source Miscellaneous
PubChem CID 12456
SMILES COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC5=C(C=C4CC3)OCO5)OC.[Cl-]
Standardized SMILES COc1ccc2cc3c4cc5OCOc5cc4CC[n+]3cc2c1OC
Molecular weight 371.8142
ALogP 1.29
H-bond donor count 0
H-bond acceptor count 5
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.42
% growth inhibition (Hom. pool) 7.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 12456
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.33||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:ADE13(YLR359W)|FD-Score:12.1|P-value:7.00E-34|Clearance:4.29||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:BRN1(YBL097W)|FD-Score:7.37|P-value:8.53E-14|Clearance:1.06||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC20(YGL116W)|FD-Score:-3.11|P-value:9.30E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:DIP2(YLR129W)|FD-Score:-3.9|P-value:4.72E-5|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:FCP1(YMR277W)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FRQ1(YDR373W)|FD-Score:3.92|P-value:4.38E-5|Clearance:0.32||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:HTB1(YDR224C)|FD-Score:4.87|P-value:5.46E-7|Clearance:0.07||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:LSG1(YGL099W)|FD-Score:11.7|P-value:1.04E-31|Clearance:4.29||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAK16(YAL025C)|FD-Score:-3.43|P-value:3.05E-4|Clearance:0||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:MTR3(YGR158C)|FD-Score:-3.28|P-value:5.19E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NMD3(YHR170W)|FD-Score:5.4|P-value:3.31E-8|Clearance:0.07||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG1(YPL093W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.03||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PRP24(YMR268C)|FD-Score:5.33|P-value:4.86E-8|Clearance:0.46||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPF2(YKR081C)|FD-Score:3.11|P-value:9.39E-4|Clearance:0.09||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPS13(YDR064W)|FD-Score:-3.45|P-value:2.76E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPT1(YKL145W)|FD-Score:-3.82|P-value:6.77E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SMC4(YLR086W)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.03||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SPC42(YKL042W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.05||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SUI3(YPL237W)|FD-Score:-3.79|P-value:7.61E-5|Clearance:0||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:THI80(YOR143C)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.06||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:TIF34(YMR146C)|FD-Score:-3.22|P-value:6.41E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:UTP15(YMR093W)|FD-Score:-4.97|P-value:3.43E-7|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP4(YDR324C)|FD-Score:-5.01|P-value:2.71E-7|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:UTP6(YDR449C)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YHR020W(YHR020W)|FD-Score:6.31|P-value:1.38E-10|Clearance:0.91||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:YJL086C(YJL086C_d)|FD-Score:4.8|P-value:7.74E-7|Clearance:0.74||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YNL150W(YNL150W_d)|FD-Score:4.06|P-value:2.45E-5|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPR136C(YPR136C_d)|FD-Score:-5.13|P-value:1.47E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ACS2(YLR153C)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.33||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:ADE13(YLR359W)|FD-Score:12.1|P-value:7.00E-34|Clearance:4.29||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:BRN1(YBL097W)|FD-Score:7.37|P-value:8.53E-14|Clearance:1.06||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC20(YGL116W)|FD-Score:-3.11|P-value:9.30E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:DIP2(YLR129W)|FD-Score:-3.9|P-value:4.72E-5|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:FCP1(YMR277W)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FRQ1(YDR373W)|FD-Score:3.92|P-value:4.38E-5|Clearance:0.32||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:HTB1(YDR224C)|FD-Score:4.87|P-value:5.46E-7|Clearance:0.07||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:LSG1(YGL099W)|FD-Score:11.7|P-value:1.04E-31|Clearance:4.29||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAK16(YAL025C)|FD-Score:-3.43|P-value:3.05E-4|Clearance:0||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:MTR3(YGR158C)|FD-Score:-3.28|P-value:5.19E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NMD3(YHR170W)|FD-Score:5.4|P-value:3.31E-8|Clearance:0.07||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG1(YPL093W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.03||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PRP24(YMR268C)|FD-Score:5.33|P-value:4.86E-8|Clearance:0.46||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPF2(YKR081C)|FD-Score:3.11|P-value:9.39E-4|Clearance:0.09||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPS13(YDR064W)|FD-Score:-3.45|P-value:2.76E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPT1(YKL145W)|FD-Score:-3.82|P-value:6.77E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SMC4(YLR086W)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.03||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SPC42(YKL042W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.05||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SUI3(YPL237W)|FD-Score:-3.79|P-value:7.61E-5|Clearance:0||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:THI80(YOR143C)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.06||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:TIF34(YMR146C)|FD-Score:-3.22|P-value:6.41E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:UTP15(YMR093W)|FD-Score:-4.97|P-value:3.43E-7|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP4(YDR324C)|FD-Score:-5.01|P-value:2.71E-7|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:UTP6(YDR449C)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YHR020W(YHR020W)|FD-Score:6.31|P-value:1.38E-10|Clearance:0.91||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:YJL086C(YJL086C_d)|FD-Score:4.8|P-value:7.74E-7|Clearance:0.74||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YNL150W(YNL150W_d)|FD-Score:4.06|P-value:2.45E-5|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPR136C(YPR136C_d)|FD-Score:-5.13|P-value:1.47E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 12456
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADE4(YMR300C)|FD-Score:4.91|P-value:4.62E-7||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AEP1(YMR064W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ALG9(YNL219C)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:APM4(YOL062C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARL1(YBR164C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP5(YNL059C)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BIK1(YCL029C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:CAF40(YNL288W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CCS1(YMR038C)|FD-Score:-3.92|P-value:4.43E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COX14(YML129C)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CYT2(YKL087C)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DAL1(YIR027C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAP2(YHR028C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:ELG1(YOR144C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:ELP3(YPL086C)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ERG3(YLR056W)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERG4(YGL012W)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GCS1(YDL226C)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEA2(YEL022W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HMI1(YOL095C)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HSP82(YPL240C)|FD-Score:4.35|P-value:6.83E-6||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:JJJ1(YNL227C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:JNM1(YMR294W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LHS1(YKL073W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MF(ALPHA)2(YGL089C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 Gene:MPA43(YNL249C)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MTF2(YDL044C)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NMA111(YNL123W)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:PHO86(YJL117W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIM1(YBL022C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PIN4(YBL051C)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RDS2(YPL133C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole Gene:REI1(YBR267W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RFM1(YOR279C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIC1(YLR039C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM101(YHL027W)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM4(YHL024W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RIM8(YGL045W)|FD-Score:9.64|P-value:2.73E-22||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RMD8(YFR048W)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Cytosolic protein required for sporulation Gene:RPL1B(YGL135W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL22A(YLR061W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:SAS2(YMR127C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family Gene:SAS4(YDR181C)|FD-Score:6.23|P-value:2.39E-10||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SAS5(YOR213C)|FD-Score:4.84|P-value:6.45E-7||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SCJ1(YMR214W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SGE1(YPR198W)|FD-Score:6.59|P-value:2.13E-11||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIR1(YKR101W)|FD-Score:5.1|P-value:1.74E-7||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SIW14(YNL032W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SNF2(YOR290C)|FD-Score:-4.01|P-value:3.05E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SUR2(YDR297W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SYS1(YJL004C)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT2(YOL020W)|FD-Score:-5.01|P-value:2.75E-7||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TRP1(YDR007W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VMA1(YDL185W)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA3(YEL027W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VMR1(YHL035C)|FD-Score:6.76|P-value:6.69E-12||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YBR074W(YBR074W_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative metalloprotease Gene:YBR090C(YBR090C_p)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YCL049C(YCL049C_p)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR095W-A(YCR095W-A_p)|FD-Score:4.31|P-value:8.24E-6||SGD DESC:Putative protein of unknown function Gene:YDR090C(YDR090C_p)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Putative protein of unknown function Gene:YDR444W(YDR444W_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGL117W(YGL117W_p)|FD-Score:-3.43|P-value:2.96E-4||SGD DESC:Putative protein of unknown function Gene:YLR236C(YLR236C_d)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR422W(YLR422W_p)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YND1(YER005W)|FD-Score:-3.8|P-value:7.27E-5||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL010W(YNL010W_p)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL228W(YNL228W_d)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YOP1(YPR028W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR072W(YOR072W_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR228C(YOR228C)|FD-Score:5.88|P-value:2.08E-9||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPL077C(YPL077C_p)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL199C(YPL199C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR015C(YPR015C_p)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR078C(YPR078C_p)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YPR197C(YPR197C_d)|FD-Score:4.85|P-value:6.03E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YTA7(YGR270W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ADE1(YAR015W)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADE4(YMR300C)|FD-Score:4.91|P-value:4.62E-7||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AEP1(YMR064W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ALG9(YNL219C)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:APM4(YOL062C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARL1(YBR164C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP5(YNL059C)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BIK1(YCL029C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:CAF40(YNL288W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CCS1(YMR038C)|FD-Score:-3.92|P-value:4.43E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COX14(YML129C)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CYT2(YKL087C)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DAL1(YIR027C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAP2(YHR028C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:ELG1(YOR144C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:ELP3(YPL086C)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ERG3(YLR056W)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERG4(YGL012W)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GCS1(YDL226C)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEA2(YEL022W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HMI1(YOL095C)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HSP82(YPL240C)|FD-Score:4.35|P-value:6.83E-6||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:JJJ1(YNL227C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:JNM1(YMR294W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LHS1(YKL073W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MF(ALPHA)2(YGL089C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 Gene:MPA43(YNL249C)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MTF2(YDL044C)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NMA111(YNL123W)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:PHO86(YJL117W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIM1(YBL022C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PIN4(YBL051C)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RDS2(YPL133C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole Gene:REI1(YBR267W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RFM1(YOR279C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIC1(YLR039C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM101(YHL027W)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM4(YHL024W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RIM8(YGL045W)|FD-Score:9.64|P-value:2.73E-22||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RMD8(YFR048W)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Cytosolic protein required for sporulation Gene:RPL1B(YGL135W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL22A(YLR061W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:SAS2(YMR127C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family Gene:SAS4(YDR181C)|FD-Score:6.23|P-value:2.39E-10||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SAS5(YOR213C)|FD-Score:4.84|P-value:6.45E-7||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SCJ1(YMR214W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SGE1(YPR198W)|FD-Score:6.59|P-value:2.13E-11||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIR1(YKR101W)|FD-Score:5.1|P-value:1.74E-7||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SIW14(YNL032W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SNF2(YOR290C)|FD-Score:-4.01|P-value:3.05E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SUR2(YDR297W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SYS1(YJL004C)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT2(YOL020W)|FD-Score:-5.01|P-value:2.75E-7||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TRP1(YDR007W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VMA1(YDL185W)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA3(YEL027W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VMR1(YHL035C)|FD-Score:6.76|P-value:6.69E-12||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YBR074W(YBR074W_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative metalloprotease Gene:YBR090C(YBR090C_p)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YCL049C(YCL049C_p)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR095W-A(YCR095W-A_p)|FD-Score:4.31|P-value:8.24E-6||SGD DESC:Putative protein of unknown function Gene:YDR090C(YDR090C_p)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Putative protein of unknown function Gene:YDR444W(YDR444W_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGL117W(YGL117W_p)|FD-Score:-3.43|P-value:2.96E-4||SGD DESC:Putative protein of unknown function Gene:YLR236C(YLR236C_d)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR422W(YLR422W_p)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YND1(YER005W)|FD-Score:-3.8|P-value:7.27E-5||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL010W(YNL010W_p)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL228W(YNL228W_d)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YOP1(YPR028W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR072W(YOR072W_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR228C(YOR228C)|FD-Score:5.88|P-value:2.08E-9||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPL077C(YPL077C_p)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL199C(YPL199C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR015C(YPR015C_p)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR078C(YPR078C_p)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YPR197C(YPR197C_d)|FD-Score:4.85|P-value:6.03E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YTA7(YGR270W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR359W12.107.00E-344.29ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YGL099W11.701.04E-314.29LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YBL097W7.378.53E-141.06BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YHR020W6.311.38E-100.91YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YHR170W5.403.31E-80.07NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YMR268C5.334.86E-80.46PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YDR224C4.875.46E-70.07HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YJL086C_d4.807.74E-70.74YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YNL150W_d4.062.45E-50.14YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YDR373W3.924.38E-50.32FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YPL093W3.601.59E-40.03NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YKL042W3.571.75E-40.05SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YLR153C3.522.16E-40.33ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YLR086W3.197.07E-40.03SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
YOR143C3.167.78E-40.06THI80Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL045W9.642.73E-22RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
YHL035C6.766.69E-12VMR1Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions
YPR198W6.592.13E-11SGE1Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
YDR181C6.232.39E-10SAS4Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
YOR228C5.882.08E-9YOR228CProtein of unknown function, localized to the mitochondrial outer membrane
YHR028C5.191.04E-7DAP2Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p
YKR101W5.101.74E-7SIR1Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin
YMR300C4.914.62E-7ADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
YPR197C_d4.856.03E-7YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR213C4.846.45E-7SAS5Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
YPR078C_p4.641.70E-6YPR078C_pPutative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible
YIR027C4.592.23E-6DAL1Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression
YHL027W4.444.58E-6RIM101Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
YPL240C4.356.83E-6HSP82Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication
YCR095W-A_p4.318.24E-6YCR095W-A_pPutative protein of unknown function

GO enrichment analysis for SGTC_2511
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3127.53E-134SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)2353160S ribosome export
0.3104.65E-132SGTC_1786berberine 21.6 μMMiscellaneous12456160S ribosome export
0.3015.32E-124SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)629413160S ribosome export
0.2353.56E-75SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.160S ribosome export
0.2283.79E-70SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.0560S ribosome export
0.2271.42E-69SGTC_8620452-0056 10.2 μMChemDiv (Drug-like library)15753960.08azole & statin
0.1985.08E-53SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.0704225azole & statin
0.1904.70E-49SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.060975660S ribosome export
0.1891.09E-48SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.082191860S ribosome export
0.1851.51E-46SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.086419860S ribosome export
0.1838.27E-46SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.10638360S ribosome export
0.1791.76E-43SGTC_21005377183 190.0 μMChembridge (Fragment library)28442020.0860S ribosome export
0.1737.60E-41SGTC_1664st014217 9.0 μMTimTec (Natural product derivative library)39827820.077777860S ribosome export
0.1694.98E-39SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.1549360S ribosome export
0.1681.26E-38SGTC_1983454-2873 16.2 μMChemDiv (Drug-like library)7109570.065789560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1786berberine21.6 μM112456Miscellaneous371.814221.2890560S ribosome export
SGTC_1827berberine7.24 μM1629413TimTec (Natural product derivative library)372.822164.3441460S ribosome export
SGTC_1829berberine14.4 μM12353TimTec (Natural product derivative library)336.361223.9620460S ribosome export
SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one54.9 μM0.304348442882TimTec (Pure natural product library)364.348143.60106redox potentiating
SGTC_2659sanguinarine24.21 μM0.29687513394149Microsource (Natural product library)429.41.13518
SGTC_2551apiole73.87 μM0.25396810659Microsource (Natural product library)222.237162.57304
SGTC_2557derrustone83.84 μM0.253521629853Microsource (Natural product library)326.300162.60106
SGTC_2492derrusnin100 μM0.251715307Microsource (Natural product library)356.326142.80107
SGTC_15382',3',6-trimethoxyflavanone63.6 μM0.2361114213774TimTec (Pure natural product library)314.332523.0505
SGTC_1083didemethylchlorpromazine sulfoxide707.39 nM0.235294398788TimTec (Natural product library)353.411623.81805redox potentiating
SGTC_15412',3',6-trimethoxyflavone64 μM0.228571688801TimTec (Pure natural product library)312.316643.08705
SGTC_1840st05536371 μM0.227273676033TimTec (Natural product derivative library)282.290663.10304