baicalein

5,6,7-trihydroxy-2-phenylchromen-4-one

Baicalein is a cell-permeable flavone, originally isolated from the roots of Scutellaria baicalensis; inhibits cellular Ca2+ uptake; may also act as an iron chelator.

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PubChem MeSH terms: Antioxidants;Enzyme Inhibitors;Prostaglandin Antagonists



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2513
Screen concentration 25.6 μM
Source Microsource (Natural product library)
PubChem CID 5281605
SMILES C1=CC=C(C=C1)C2=CC(=O)C3=C(C(=C(C=C3O2)O)O)O
Standardized SMILES Oc1cc2OC(=CC(=O)c2c(O)c1O)c3ccccc3
Molecular weight 270.2369
ALogP 2.41
H-bond donor count 3
H-bond acceptor count 5
Response signature calcium & mitochondrial duress

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.5
% growth inhibition (Hom. pool) 4.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5281605
Download HIP data (tab-delimited text)  (excel)
Gene:CDC37(YDR168W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.38||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:GCD10(YNL062C)|FD-Score:4.47|P-value:3.99E-6|Clearance:0.39||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GPI8(YDR331W)|FD-Score:-3.33|P-value:4.34E-4|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:IRR1(YIL026C)|FD-Score:10.6|P-value:2.19E-26|Clearance:6.1||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LSG1(YGL099W)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAS1(YLR163C)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.07||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCM5(YLR274W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.04||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MED7(YOL135C)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NUS1(YDL193W)|FD-Score:3.27|P-value:5.29E-4|Clearance:0.01||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE7(YBL041W)|FD-Score:3.21|P-value:6.57E-4|Clearance:0.06||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RER2(YBR002C)|FD-Score:12.9|P-value:2.30E-38|Clearance:6.1||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPA43(YOR340C)|FD-Score:3.28|P-value:5.24E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:TAF7(YMR227C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.42||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:UTP20(YBL004W)|FD-Score:-3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:CDC37(YDR168W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.38||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:GCD10(YNL062C)|FD-Score:4.47|P-value:3.99E-6|Clearance:0.39||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GPI8(YDR331W)|FD-Score:-3.33|P-value:4.34E-4|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:IRR1(YIL026C)|FD-Score:10.6|P-value:2.19E-26|Clearance:6.1||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LSG1(YGL099W)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAS1(YLR163C)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.07||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCM5(YLR274W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.04||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MED7(YOL135C)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NUS1(YDL193W)|FD-Score:3.27|P-value:5.29E-4|Clearance:0.01||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE7(YBL041W)|FD-Score:3.21|P-value:6.57E-4|Clearance:0.06||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RER2(YBR002C)|FD-Score:12.9|P-value:2.30E-38|Clearance:6.1||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPA43(YOR340C)|FD-Score:3.28|P-value:5.24E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:TAF7(YMR227C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.42||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:UTP20(YBL004W)|FD-Score:-3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5281605
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:14|P-value:1.07E-44||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIM46(YHR199C)|FD-Score:4.39|P-value:5.72E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARG82(YDR173C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG1(YGL180W)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:BAR1(YIL015W)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:BUL2(YML111W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CAD1(YDR423C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CDC55(YGL190C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CIR2(YOR356W)|FD-Score:-5.74|P-value:4.76E-9||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:DDR48(YMR173W)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress Gene:DIA1(YMR316W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DNF1(YER166W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DPB3(YBR278W)|FD-Score:6.99|P-value:1.34E-12||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:DSK2(YMR276W)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:ENO1(YGR254W)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FAR7(YFR008W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FRE5(YOR384W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FZF1(YGL254W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:FZO1(YBR179C)|FD-Score:6.27|P-value:1.78E-10||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GDT1(YBR187W_p)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GEP4(YHR100C)|FD-Score:7.94|P-value:1.02E-15||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GGC1(YDL198C)|FD-Score:4.89|P-value:5.15E-7||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GMC2(YLR445W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:IMP2'(YIL154C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISA2(YPR067W)|FD-Score:7.26|P-value:1.97E-13||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:LEO1(YOR123C)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LHP1(YDL051W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen Gene:LYS2(YBR115C)|FD-Score:-4.35|P-value:6.84E-6||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MAC1(YMR021C)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDJ1(YFL016C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MGM1(YOR211C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MPD1(YOR288C)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRPS12(YNR036C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSN1(YOL116W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MTM1(YGR257C)|FD-Score:6.87|P-value:3.20E-12||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:MUM3(YOR298W)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NCE101(YJL205C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NMA111(YNL123W)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NPP1(YCR026C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OM14(YBR230C)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:PBY1(YBR094W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress Gene:PET309(YLR067C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PMP1(YCR024C-A)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PPA2(YMR267W)|FD-Score:4.5|P-value:3.45E-6||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:RDL1(YOR285W)|FD-Score:-3.74|P-value:9.22E-5||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIM8(YGL045W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPL14A(YKL006W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPN10(YHR200W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RSM22(YKL155C)|FD-Score:11.7|P-value:9.12E-32||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:SEH1(YGL100W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SIW14(YNL032W)|FD-Score:-3.83|P-value:6.45E-5||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SOV1(YMR066W)|FD-Score:-3.96|P-value:3.76E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SPH1(YLR313C)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SRB8(YCR081W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSQ1(YLR369W)|FD-Score:8.83|P-value:5.19E-19||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:SUV3(YPL029W)|FD-Score:4.51|P-value:3.18E-6||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SYH1(YPL105C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TIS11(YLR136C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TRX3(YCR083W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:UFD4(YKL010C)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:VMA7(YGR020C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS34(YLR240W)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS68(YOL129W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YBR141C(YBR141C_p)|FD-Score:-4.75|P-value:1.00E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YDR509W(YDR509W_d)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER134C(YER134C)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YFL019C(YFL019C_d)|FD-Score:3.96|P-value:3.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YIL067C(YIL067C_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YLR171W(YLR171W_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:-4.17|P-value:1.52E-5||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL143C(YNL143C_p)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR186W(YOR186W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR228C(YOR228C)|FD-Score:5.65|P-value:8.02E-9||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPR003C(YPR003C_p)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPT53(YNL093W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:YRR1(YOR162C)|FD-Score:5.68|P-value:6.67E-9||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZRG8(YER033C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ACO1(YLR304C)|FD-Score:14|P-value:1.07E-44||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIM46(YHR199C)|FD-Score:4.39|P-value:5.72E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARG82(YDR173C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG1(YGL180W)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:BAR1(YIL015W)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:BUL2(YML111W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CAD1(YDR423C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CDC55(YGL190C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CIR2(YOR356W)|FD-Score:-5.74|P-value:4.76E-9||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:DDR48(YMR173W)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress Gene:DIA1(YMR316W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DNF1(YER166W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DPB3(YBR278W)|FD-Score:6.99|P-value:1.34E-12||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:DSK2(YMR276W)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:ENO1(YGR254W)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FAR7(YFR008W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FRE5(YOR384W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FZF1(YGL254W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:FZO1(YBR179C)|FD-Score:6.27|P-value:1.78E-10||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GDT1(YBR187W_p)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GEP4(YHR100C)|FD-Score:7.94|P-value:1.02E-15||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GGC1(YDL198C)|FD-Score:4.89|P-value:5.15E-7||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GMC2(YLR445W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:IMP2'(YIL154C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISA2(YPR067W)|FD-Score:7.26|P-value:1.97E-13||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:LEO1(YOR123C)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LHP1(YDL051W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen Gene:LYS2(YBR115C)|FD-Score:-4.35|P-value:6.84E-6||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MAC1(YMR021C)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDJ1(YFL016C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MGM1(YOR211C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MPD1(YOR288C)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRPS12(YNR036C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSN1(YOL116W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MTM1(YGR257C)|FD-Score:6.87|P-value:3.20E-12||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:MUM3(YOR298W)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NCE101(YJL205C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NMA111(YNL123W)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NPP1(YCR026C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OM14(YBR230C)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:PBY1(YBR094W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress Gene:PET309(YLR067C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PMP1(YCR024C-A)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PPA2(YMR267W)|FD-Score:4.5|P-value:3.45E-6||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:RDL1(YOR285W)|FD-Score:-3.74|P-value:9.22E-5||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIM8(YGL045W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPL14A(YKL006W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPN10(YHR200W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RSM22(YKL155C)|FD-Score:11.7|P-value:9.12E-32||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:SEH1(YGL100W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SIW14(YNL032W)|FD-Score:-3.83|P-value:6.45E-5||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SOV1(YMR066W)|FD-Score:-3.96|P-value:3.76E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SPH1(YLR313C)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SRB8(YCR081W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSQ1(YLR369W)|FD-Score:8.83|P-value:5.19E-19||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:SUV3(YPL029W)|FD-Score:4.51|P-value:3.18E-6||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SYH1(YPL105C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TIS11(YLR136C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TRX3(YCR083W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:UFD4(YKL010C)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:VMA7(YGR020C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS34(YLR240W)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS68(YOL129W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YBR141C(YBR141C_p)|FD-Score:-4.75|P-value:1.00E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YDR509W(YDR509W_d)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER134C(YER134C)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YFL019C(YFL019C_d)|FD-Score:3.96|P-value:3.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YIL067C(YIL067C_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YLR171W(YLR171W_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:-4.17|P-value:1.52E-5||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL143C(YNL143C_p)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR186W(YOR186W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR228C(YOR228C)|FD-Score:5.65|P-value:8.02E-9||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPR003C(YPR003C_p)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPT53(YNL093W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:YRR1(YOR162C)|FD-Score:5.68|P-value:6.67E-9||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZRG8(YER033C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C12.902.30E-386.10RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YIL026C10.602.19E-266.10IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YNL062C4.473.99E-60.39GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YDR168W4.072.34E-50.38CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YMR227C3.691.11E-40.42TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YOR340C3.285.24E-40.00RPA43RNA polymerase I subunit A43
YDL193W3.275.29E-40.01NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YLR274W3.275.47E-40.04MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YOL135C3.226.31E-40.01MED7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YBL041W3.216.57E-40.06PRE7Beta 6 subunit of the 20S proteasome
YLR163C3.167.98E-40.07MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YHR058C3.080.001020.00MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
YBR004C3.080.001030.08GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YKL104C3.000.001350.32GFA1Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
YPR035W2.680.003640.04GLN1Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR304C14.001.07E-44ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YKL155C11.709.12E-32RSM22Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
YLR369W8.835.19E-19SSQ1Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia
YHR100C7.941.02E-15GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YPR067W7.261.97E-13ISA2Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
YBR278W6.991.34E-12DPB3Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
YGR257C6.873.20E-12MTM1Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor
YBR179C6.271.78E-10FZO1Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
YOR162C5.686.67E-9YRR1Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
YOR228C5.658.02E-9YOR228CProtein of unknown function, localized to the mitochondrial outer membrane
YFL016C5.121.53E-7MDJ1Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
YDL198C4.895.15E-7GGC1Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family
YGL254W4.741.09E-6FZF1Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress
YGL180W4.731.11E-6ATG1Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p
YPL029W4.513.18E-6SUV3ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron

GO enrichment analysis for SGTC_2513
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2151.06E-62SGTC_11881403-0014 4.6 μMChemDiv (Drug-like library)96148670.046875mitochondrial stress
0.1863.09E-47SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.16TRP & mitochondrial translation
0.1606.27E-35SGTC_12600700-1617 7.8 μMChemDiv (Drug-like library)28530780.0862069calcium & mitochondrial duress
0.1584.22E-34SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.16TRP & mitochondrial translation
0.1532.11E-32SGTC_1742st038235 28.9 μMTimTec (Natural product derivative library)5641030.125mitochondrial stress
0.1352.68E-25SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0983607mitochondrial stress
0.1313.90E-24SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.0769231TRP & mitochondrial translation
0.1315.71E-24SGTC_2516gossypol 71.5 μMTimTec (Pure natural product library)35030.137931
0.1292.78E-23SGTC_2759disulfiram 3.4 μMMiscellaneous3117NA
0.1284.18E-23SGTC_2615purpurogallin-4-carboxylic acid 100.0 μMMicrosource (Natural product library)2693150.28redox potentiating
0.1251.14E-21SGTC_6140462-0418 107.0 μMChemDiv (Drug-like library)51883030.0666667
0.1241.38E-21SGTC_5961155-0152 116.0 μMChemDiv (Drug-like library)2803130.0892857TRP & mitochondrial translation
0.1232.56E-21SGTC_1863455-3465 67.3 μMChemDiv (Drug-like library)7552780.125
0.1226.53E-21SGTC_2250alverine citrate 130.0 μMMiscellaneous217180.12
0.1189.62E-20SGTC_1954234-0266 16.7 μMChemDiv (Drug-like library)23033540.1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2515chrysin20.77 μM0.6923085281607Microsource (Natural product library)254.23752.65224RPP1 & pyrimidine depletion
SGTC_1834st05598152.8 μM0.690476471719TimTec (Natural product derivative library)284.263482.63625RPP1 & pyrimidine depletion
SGTC_1965Pectolinarigenin58.4 μM0.5531915320438TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_1529apigenin74 μM0.5476195280443TimTec (Pure natural product library)270.23692.4135
SGTC_24977-hydroxyflavone100 μM0.5476195281894Microsource (Natural product library)238.23812.89413
SGTC_1852st05625431.4 μM0.521739442583TimTec (Natural product derivative library)312.316643.08705
SGTC_1959st07709155.8 μM0.521739821356TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_2550chrysin dimethyl ether71.87 μM0.52173988881Microsource (Natural product library)282.290663.10304
SGTC_15324',5'-dihydroxyflavone78.7 μM0.511628165521TimTec (Pure natural product library)254.23752.65224Golgi
SGTC_25787,8-dihydroxyflavone64.44 μM0.5116281880Microsource (Natural product library)254.23752.65224