totarol

(4bS)-4b,8,8-trimethyl-1-propan-2-yl-5,6,7,8a,9,10-hexahydrophenanthren-2-ol

An antibacterial membrane disruptor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2517
Screen concentration 10.0 μM
Source Microsource (Natural product library)
PubChem CID 460178
SMILES CC(C)C1=C(C=CC2=C1CCC3C2(CCCC3(C)C)C)O
Standardized SMILES CC(C)c1c(O)ccc2c1CCC3C(C)(C)CCCC23C
Molecular weight 286.4516
ALogP 6
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.79
% growth inhibition (Hom. pool) 3.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 460178
Download HIP data (tab-delimited text)  (excel)
Gene:BRN1(YBL097W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.06||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC25(YLR310C)|FD-Score:4.79|P-value:8.32E-7|Clearance:0.17||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CLP1(YOR250C)|FD-Score:-3.87|P-value:5.52E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:ERG12(YMR208W)|FD-Score:-4.32|P-value:7.97E-6|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG26(YGL001C)|FD-Score:15.8|P-value:9.84E-57|Clearance:11.04||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FAD1(YDL045C)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.13||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:GCR1(YPL075W)|FD-Score:3.95|P-value:3.86E-5|Clearance:0.44||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:POL2(YNL262W)|FD-Score:-3.81|P-value:7.00E-5|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PRP28(YDR243C)|FD-Score:-3.56|P-value:1.83E-4|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PRP6(YBR055C)|FD-Score:4.01|P-value:3.03E-5|Clearance:0.06||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPN2(YIL075C)|FD-Score:4.09|P-value:2.17E-5|Clearance:0.08||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RTP1(YMR185W_p)|FD-Score:-3.16|P-value:7.83E-4|Clearance:0||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SEC10(YLR166C)|FD-Score:-3.59|P-value:1.66E-4|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SRP1(YNL189W)|FD-Score:-3.22|P-value:6.39E-4|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TAD3(YLR316C)|FD-Score:-4.5|P-value:3.38E-6|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TFC3(YAL001C)|FD-Score:4.62|P-value:1.93E-6|Clearance:0.53||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRE2(YOR256C)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.14||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:UBA2(YDR390C)|FD-Score:3.15|P-value:8.23E-4|Clearance:0.08||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:YJL202C(YJL202C_d)|FD-Score:3.18|P-value:7.41E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex Gene:BRN1(YBL097W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.06||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC25(YLR310C)|FD-Score:4.79|P-value:8.32E-7|Clearance:0.17||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CLP1(YOR250C)|FD-Score:-3.87|P-value:5.52E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:ERG12(YMR208W)|FD-Score:-4.32|P-value:7.97E-6|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG26(YGL001C)|FD-Score:15.8|P-value:9.84E-57|Clearance:11.04||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FAD1(YDL045C)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.13||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:GCR1(YPL075W)|FD-Score:3.95|P-value:3.86E-5|Clearance:0.44||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:POL2(YNL262W)|FD-Score:-3.81|P-value:7.00E-5|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PRP28(YDR243C)|FD-Score:-3.56|P-value:1.83E-4|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PRP6(YBR055C)|FD-Score:4.01|P-value:3.03E-5|Clearance:0.06||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPN2(YIL075C)|FD-Score:4.09|P-value:2.17E-5|Clearance:0.08||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RTP1(YMR185W_p)|FD-Score:-3.16|P-value:7.83E-4|Clearance:0||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SEC10(YLR166C)|FD-Score:-3.59|P-value:1.66E-4|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SRP1(YNL189W)|FD-Score:-3.22|P-value:6.39E-4|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TAD3(YLR316C)|FD-Score:-4.5|P-value:3.38E-6|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TFC3(YAL001C)|FD-Score:4.62|P-value:1.93E-6|Clearance:0.53||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRE2(YOR256C)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.14||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:UBA2(YDR390C)|FD-Score:3.15|P-value:8.23E-4|Clearance:0.08||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:YJL202C(YJL202C_d)|FD-Score:3.18|P-value:7.41E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 460178
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:-4.3|P-value:8.46E-6||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE1(YAR015W)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADE17(YMR120C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:APE3(YBR286W)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ARG7(YMR062C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ARO3(YDR035W)|FD-Score:5.22|P-value:8.86E-8||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARP1(YHR129C)|FD-Score:7.1|P-value:6.44E-13||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ARP5(YNL059C)|FD-Score:-4.33|P-value:7.37E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATO3(YDR384C)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:ATP3(YBR039W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BNA1(YJR025C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BNA3(YJL060W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BSC5(YNR069C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CAM1(YPL048W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CDC73(YLR418C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CRP1(YHR146W)|FD-Score:4.83|P-value:6.88E-7||SGD DESC:Protein that binds to cruciform DNA structures Gene:DGR2(YKL121W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DSS4(YPR017C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EGT2(YNL327W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:ERI1(YPL096C-A)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FRK1(YPL141C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FUI1(YBL042C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GSP2(YOR185C)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HOT1(YMR172W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p Gene:HST3(YOR025W)|FD-Score:-3.72|P-value:9.92E-5||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:KTI12(YKL110C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:KTR6(YPL053C)|FD-Score:4.27|P-value:9.66E-6||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:MBB1(YJL199C_d)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MDM32(YOR147W)|FD-Score:-3.39|P-value:3.43E-4||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:NAB6(YML117W)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NKP1(YDR383C)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NUP53(YMR153W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OPI1(YHL020C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PDR1(YGL013C)|FD-Score:6.85|P-value:3.65E-12||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR16(YNL231C)|FD-Score:5.04|P-value:2.32E-7||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PSY2(YNL201C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RIM8(YGL045W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:-3.8|P-value:7.34E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RLF2(YPR018W)|FD-Score:5.23|P-value:8.61E-8||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL2B(YIL018W)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPS12(YOR369C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RSA1(YPL193W)|FD-Score:5.33|P-value:5.05E-8||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RXT2(YBR095C)|FD-Score:5.15|P-value:1.34E-7||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SEC22(YLR268W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SGF73(YGL066W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIN3(YOL004W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SIR1(YKR101W)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SPO12(YHR152W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SPT23(YKL020C)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SPT4(YGR063C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRB8(YCR081W)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRC1(YML034W)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSA3(YBL075C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TDP1(YBR223C)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TIM8(YJR135W-A)|FD-Score:-3.83|P-value:6.41E-5||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TRM7(YBR061C)|FD-Score:4.28|P-value:9.37E-6||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:URM1(YIL008W)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC7(YNL054W)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VIK1(YPL253C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS45(YGL095C)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:XYL2(YLR070C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBL012C(YBL012C_d)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR141C(YBR141C_p)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCL042W(YCL042W_p)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR149C(YDR149C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Putative protein of unknown function Gene:YDR239C(YDR239C)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YDR444W(YDR444W_p)|FD-Score:-3.16|P-value:7.80E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGR064W(YGR064W_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL166C(YIL166C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJR018W(YJR018W_d)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR079W(YJR079W_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YMR294W-A(YMR294W-A_d)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL205C(YNL205C_d)|FD-Score:4.8|P-value:7.99E-7||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR1(YGR281W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR131C(YOR131C_p)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YPR130C(YPR130C_d)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZRG8(YER033C)|FD-Score:-6.39|P-value:8.52E-11||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ACB1(YGR037C)|FD-Score:-4.3|P-value:8.46E-6||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE1(YAR015W)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADE17(YMR120C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:APE3(YBR286W)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ARG7(YMR062C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ARO3(YDR035W)|FD-Score:5.22|P-value:8.86E-8||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARP1(YHR129C)|FD-Score:7.1|P-value:6.44E-13||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ARP5(YNL059C)|FD-Score:-4.33|P-value:7.37E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATO3(YDR384C)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:ATP3(YBR039W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BNA1(YJR025C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BNA3(YJL060W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BSC5(YNR069C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CAM1(YPL048W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CDC73(YLR418C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CRP1(YHR146W)|FD-Score:4.83|P-value:6.88E-7||SGD DESC:Protein that binds to cruciform DNA structures Gene:DGR2(YKL121W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DSS4(YPR017C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EGT2(YNL327W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:ERI1(YPL096C-A)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FRK1(YPL141C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FUI1(YBL042C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GSP2(YOR185C)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HOT1(YMR172W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p Gene:HST3(YOR025W)|FD-Score:-3.72|P-value:9.92E-5||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:KTI12(YKL110C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:KTR6(YPL053C)|FD-Score:4.27|P-value:9.66E-6||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:MBB1(YJL199C_d)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MDM32(YOR147W)|FD-Score:-3.39|P-value:3.43E-4||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:NAB6(YML117W)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NKP1(YDR383C)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NUP53(YMR153W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OPI1(YHL020C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PDR1(YGL013C)|FD-Score:6.85|P-value:3.65E-12||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR16(YNL231C)|FD-Score:5.04|P-value:2.32E-7||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PSY2(YNL201C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RIM8(YGL045W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:-3.8|P-value:7.34E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RLF2(YPR018W)|FD-Score:5.23|P-value:8.61E-8||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL2B(YIL018W)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPS12(YOR369C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RSA1(YPL193W)|FD-Score:5.33|P-value:5.05E-8||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RXT2(YBR095C)|FD-Score:5.15|P-value:1.34E-7||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SEC22(YLR268W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SGF73(YGL066W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIN3(YOL004W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SIR1(YKR101W)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SPO12(YHR152W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SPT23(YKL020C)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SPT4(YGR063C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRB8(YCR081W)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRC1(YML034W)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSA3(YBL075C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TDP1(YBR223C)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TIM8(YJR135W-A)|FD-Score:-3.83|P-value:6.41E-5||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TRM7(YBR061C)|FD-Score:4.28|P-value:9.37E-6||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:URM1(YIL008W)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC7(YNL054W)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VIK1(YPL253C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS45(YGL095C)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:XYL2(YLR070C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBL012C(YBL012C_d)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR141C(YBR141C_p)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCL042W(YCL042W_p)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR149C(YDR149C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Putative protein of unknown function Gene:YDR239C(YDR239C)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YDR444W(YDR444W_p)|FD-Score:-3.16|P-value:7.80E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGR064W(YGR064W_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL166C(YIL166C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJR018W(YJR018W_d)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR079W(YJR079W_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YMR294W-A(YMR294W-A_d)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL205C(YNL205C_d)|FD-Score:4.8|P-value:7.99E-7||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR1(YGR281W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR131C(YOR131C_p)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YPR130C(YPR130C_d)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZRG8(YER033C)|FD-Score:-6.39|P-value:8.52E-11||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL001C15.809.84E-5711.00ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YLR310C4.798.32E-70.17CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YAL001C4.621.93E-60.53TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YIL075C4.092.17E-50.08RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YBR055C4.013.03E-50.06PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YPL075W3.953.86E-50.44GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YDL045C3.512.25E-40.13FAD1Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
YOR256C3.383.63E-40.14TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YBL097W3.246.03E-40.06BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YJL202C_d3.187.41E-40.03YJL202C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
YDR390C3.158.23E-40.08UBA2Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YNR053C3.070.001070.13NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YFR004W2.940.001660.13RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YKL125W2.810.002490.02RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YBR196C2.790.002660.10PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR129C7.106.44E-13ARP1Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
YGL013C6.853.65E-12PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YPL193W5.335.05E-8RSA1Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
YPR018W5.238.61E-8RLF2Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription
YDR035W5.228.86E-8ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YBR095C5.151.34E-7RXT2Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth
YNL231C5.042.32E-7PDR16Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
YHR146W4.836.88E-7CRP1Protein that binds to cruciform DNA structures
YNL205C_d4.807.99E-7YNL205C_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR081W4.721.16E-6SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YBR286W4.621.96E-6APE3Vacuolar aminopeptidase Y, processed to mature form by Prb1p
YMR294W-A_d4.562.51E-6YMR294W-A_dDubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents
YIL008W4.356.75E-6URM1Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
YBR061C4.289.37E-6TRM72'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively
YPL053C4.279.66E-6KTR6Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2517
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0951.94E-13SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.163636PDR1
0.0912.21E-12SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0540541PDR1
0.0904.80E-12SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0434783PDR1
0.0899.09E-12SGTC_489paxilline 115.0 μMICCB bioactive library1050080.136364PDR1
0.0881.24E-11SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.111111PDR1
0.0857.04E-11SGTC_1533dihydroergotamine mesylate 29.4 μMTimTec (Pure natural product library)711710.0973451DNA intercalators
0.0841.01E-10SGTC_5433844-1095 191.0 μMChemDiv (Drug-like library)42307030.0789474ERG2
0.0831.40E-10SGTC_196k015-0023 18.1 μMChemDiv (Drug-like library)52119980.04PDR1
0.0814.20E-10SGTC_676falcarinol 140.0 μMMiscellaneous33220.0428571
0.0741.07E-8SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0681818PDR1
0.0714.17E-8SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.134146PDR1
0.0714.96E-8SGTC_1934st074720 32.6 μMTimTec (Natural product derivative library)172509920.0526316
0.0715.46E-8SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.0641026
0.0715.91E-8SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.0410959PDR1
0.0715.81E-8SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0645161PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_260nsc-652383.99 μM0.3965523246252Miscellaneous407.588183.84623endomembrane recycling
SGTC_249418-aminoabieta-8,11,13-triene sulfate3.66 μM0.39655216725076Microsource (Natural product library)383.545324.25835
SGTC_2536dehydroabietamide32.04 μM0.3833333983547Microsource (Natural product library)299.450364.76211
SGTC_2616abietic acid100 μM0.2537316708678Microsource (Natural product library)302.4515.09312
SGTC_1125tnp002481.33 μM0.24561424207736TimTec (Natural product library)250.376443.49502
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.2413796708760Microsource (Natural product library)216.31873.35611
SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one66.67 μM0.2295086708561Microsource (Natural product library)228.32943.65701
SGTC_406estrone100 μM0.2121215870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.212121698TimTec (Pure natural product library)270.366083.93812DNA intercalators
SGTC_2519cryptotanshinone88.04 μM0.205882160254Microsource (Natural product library)296.36033.76103heme biosynthesis & mitochondrial translocase