biochanin a

5,7-dihydroxy-3-(4-methoxyphenyl)chromen-4-one

A possible chelator.

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PubChem MeSH terms: Anticarcinogenic Agents;Phytoestrogens



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2522
Screen concentration 4.9 μM
Source Microsource (Natural product library)
PubChem CID 5280373
SMILES COC1=CC=C(C=C1)C2=COC3=CC(=CC(=C3C2=O)O)O
Standardized SMILES COc1ccc(cc1)C2=COc3cc(O)cc(O)c3C2=O
Molecular weight 284.2635
ALogP 2.37
H-bond donor count 2
H-bond acceptor count 5
Response signature RNA pol III & RNase P/MRP

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.22
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5280373
Download HIP data (tab-delimited text)  (excel)
Gene:BRN1(YBL097W)|FD-Score:-4.29|P-value:9.00E-6|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CWC2(YDL209C)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GCD1(YOR260W)|FD-Score:5.58|P-value:1.21E-8|Clearance:0.02||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.03||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:MCM4(YPR019W)|FD-Score:5.55|P-value:1.40E-8|Clearance:0.03||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:NFS1(YCL017C)|FD-Score:5.22|P-value:8.79E-8|Clearance:0.5||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:PRP6(YBR055C)|FD-Score:-3.19|P-value:7.05E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RET1(YOR207C)|FD-Score:12|P-value:2.03E-33|Clearance:4.19||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB8(YOR224C)|FD-Score:4.39|P-value:5.60E-6|Clearance:0.49||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC17(YJL011C)|FD-Score:5.53|P-value:1.63E-8|Clearance:0.3||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPC53(YDL150W)|FD-Score:7.8|P-value:3.03E-15|Clearance:0.55||SGD DESC:RNA polymerase III subunit C53 Gene:RPC82(YPR190C)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.26||SGD DESC:RNA polymerase III subunit C82 Gene:RPL32(YBL092W)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.09||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPO31(YOR116C)|FD-Score:15.4|P-value:6.17E-54|Clearance:4.19||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:4.47|P-value:3.96E-6|Clearance:0.07||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:YDL152W(YDL152W_d)|FD-Score:7.25|P-value:2.03E-13|Clearance:1.68||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YHR020W(YHR020W)|FD-Score:3.9|P-value:4.74E-5|Clearance:0.73||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:YPI1(YFR003C)|FD-Score:-4.45|P-value:4.38E-6|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:BRN1(YBL097W)|FD-Score:-4.29|P-value:9.00E-6|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CWC2(YDL209C)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GCD1(YOR260W)|FD-Score:5.58|P-value:1.21E-8|Clearance:0.02||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.03||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:MCM4(YPR019W)|FD-Score:5.55|P-value:1.40E-8|Clearance:0.03||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:NFS1(YCL017C)|FD-Score:5.22|P-value:8.79E-8|Clearance:0.5||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:PRP6(YBR055C)|FD-Score:-3.19|P-value:7.05E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RET1(YOR207C)|FD-Score:12|P-value:2.03E-33|Clearance:4.19||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB8(YOR224C)|FD-Score:4.39|P-value:5.60E-6|Clearance:0.49||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC17(YJL011C)|FD-Score:5.53|P-value:1.63E-8|Clearance:0.3||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPC53(YDL150W)|FD-Score:7.8|P-value:3.03E-15|Clearance:0.55||SGD DESC:RNA polymerase III subunit C53 Gene:RPC82(YPR190C)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.26||SGD DESC:RNA polymerase III subunit C82 Gene:RPL32(YBL092W)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.09||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPO31(YOR116C)|FD-Score:15.4|P-value:6.17E-54|Clearance:4.19||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:4.47|P-value:3.96E-6|Clearance:0.07||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:YDL152W(YDL152W_d)|FD-Score:7.25|P-value:2.03E-13|Clearance:1.68||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YHR020W(YHR020W)|FD-Score:3.9|P-value:4.74E-5|Clearance:0.73||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:YPI1(YFR003C)|FD-Score:-4.45|P-value:4.38E-6|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5280373
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACM1(YPL267W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ALG9(YNL219C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:ASM4(YDL088C)|FD-Score:-3.77|P-value:8.06E-5||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATG23(YLR431C)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:ATG7(YHR171W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BOP2(YLR267W_p)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Protein of unknown function Gene:CLC1(YGR167W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLN3(YAL040C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CPR8(YNR028W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:EGD1(YPL037C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:FET4(YMR319C)|FD-Score:4.44|P-value:4.47E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FIN1(YDR130C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:GLC3(YEL011W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:HPT1(YDR399W)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:LAS21(YJL062W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MRPL33(YMR286W)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSN1(YOL116W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NTO1(YPR031W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PDC5(YLR134W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEP7(YDR323C)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PER1(YCR044C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET18(YCR020C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PFK1(YGR240C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIH1(YHR034C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PRY2(YKR013W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RIT1(YMR283C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RMD9(YGL107C)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPL19A(YBR084C-A)|FD-Score:-3.83|P-value:6.47E-5||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM22(YKL155C)|FD-Score:8.73|P-value:1.23E-18||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:SEH1(YGL100W)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SIS2(YKR072C)|FD-Score:6.47|P-value:4.88E-11||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SOV1(YMR066W)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SPO12(YHR152W)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SSD1(YDR293C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSM4(YIL030C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:STP2(YHR006W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SYN8(YAL014C)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TOS6(YNL300W_p)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPN1(YGL186C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBA4(YHR111W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBX7(YBR273C)|FD-Score:3.75|P-value:9.02E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:URA4(YLR420W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:URA5(YML106W)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:VMS1(YDR049W)|FD-Score:3.8|P-value:7.23E-5||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YBL062W(YBL062W_d)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL075W(YCL075W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDL023C(YDL023C_d)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL041W(YDL041W_d)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YGK3(YOL128C)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGR201C(YGR201C_p)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Putative protein of unknown function Gene:YGR266W(YGR266W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YHR182W(YHR182W_p)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIL163C(YIL163C_p)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKR073C(YKR073C_d)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR108C(YLR108C_p)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YOR343C(YOR343C_d)|FD-Score:4.91|P-value:4.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR084W(YPR084W_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function Gene:ACB1(YGR037C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACM1(YPL267W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ALG9(YNL219C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:ASM4(YDL088C)|FD-Score:-3.77|P-value:8.06E-5||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATG23(YLR431C)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:ATG7(YHR171W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BOP2(YLR267W_p)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Protein of unknown function Gene:CLC1(YGR167W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLN3(YAL040C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CPR8(YNR028W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:EGD1(YPL037C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:FET4(YMR319C)|FD-Score:4.44|P-value:4.47E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FIN1(YDR130C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:GLC3(YEL011W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:HPT1(YDR399W)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:LAS21(YJL062W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MRPL33(YMR286W)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSN1(YOL116W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NTO1(YPR031W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PDC5(YLR134W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEP7(YDR323C)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PER1(YCR044C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET18(YCR020C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PFK1(YGR240C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIH1(YHR034C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PRY2(YKR013W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RIT1(YMR283C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RMD9(YGL107C)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPL19A(YBR084C-A)|FD-Score:-3.83|P-value:6.47E-5||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM22(YKL155C)|FD-Score:8.73|P-value:1.23E-18||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:SEH1(YGL100W)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SIS2(YKR072C)|FD-Score:6.47|P-value:4.88E-11||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SOV1(YMR066W)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SPO12(YHR152W)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SSD1(YDR293C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSM4(YIL030C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:STP2(YHR006W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SYN8(YAL014C)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TOS6(YNL300W_p)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPN1(YGL186C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBA4(YHR111W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBX7(YBR273C)|FD-Score:3.75|P-value:9.02E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:URA4(YLR420W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:URA5(YML106W)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:VMS1(YDR049W)|FD-Score:3.8|P-value:7.23E-5||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YBL062W(YBL062W_d)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL075W(YCL075W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDL023C(YDL023C_d)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL041W(YDL041W_d)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YGK3(YOL128C)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGR201C(YGR201C_p)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Putative protein of unknown function Gene:YGR266W(YGR266W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YHR182W(YHR182W_p)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIL163C(YIL163C_p)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKR073C(YKR073C_d)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR108C(YLR108C_p)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YOR343C(YOR343C_d)|FD-Score:4.91|P-value:4.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR084W(YPR084W_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR116C15.406.17E-544.19RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YOR207C12.002.03E-334.19RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDL150W7.803.03E-150.55RPC53RNA polymerase III subunit C53
YDL152W_d7.252.03E-131.68YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YOR260W5.581.21E-80.02GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YPR019W5.551.40E-80.03MCM4Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
YJL011C5.531.63E-80.30RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YCL017C5.228.79E-80.50NFS1Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
YPR190C4.721.16E-60.26RPC82RNA polymerase III subunit C82
YHR062C4.473.96E-60.07RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR224C4.395.60E-60.49RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YHR020W3.904.74E-50.73YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YDR211W3.187.46E-40.03GCD6Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
YBL092W3.158.14E-40.09RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YMR197C3.060.001100.14VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL155C8.731.23E-18RSM22Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
YKR072C6.474.88E-11SIS2Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
YOR343C_d4.914.46E-7YOR343C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLR431C4.721.18E-6ATG23Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
YKR073C_d4.641.75E-6YKR073C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR319C4.444.47E-6FET4Low-affinity Fe(II) transporter of the plasma membrane
YGL186C4.211.28E-5TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YHR006W4.201.32E-5STP2Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
YEL011W4.161.58E-5GLC3Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YMR066W4.102.02E-5SOV1Mitochondrial protein of unknown function
YOR188W4.052.51E-5MSB1Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
YGL100W3.934.31E-5SEH1Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1
YCL075W3.807.16E-5YCL075WPseudogene: encodes fragment of Ty Pol protein
YDR049W3.807.23E-5VMS1Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
YBR273C3.759.02E-5UBX7UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2522
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4006.42E-226SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.131579RNA pol III & RNase P/MRP
0.3782.16E-200SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.177419RNA pol III & RNase P/MRP
0.3369.22E-156SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.137255RNA pol III & RNase P/MRP
0.3356.48E-155SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.126984RNA pol III & RNase P/MRP
0.2971.92E-120SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.133333RNA pol III & RNase P/MRP
0.2816.49E-108SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.557692RNA pol III & RNase P/MRP
0.2283.23E-70SGTC_23067482397 179.5 μMChembridge (Fragment library)8914480.126984RPP1 & pyrimidine depletion
0.2022.80E-55SGTC_6781218-2179 85.9 μMChemDiv (Drug-like library)68200810.147059RNA pol III & RNase P/MRP
0.2002.72E-54SGTC_8291635-0120 117.0 μMChemDiv (Drug-like library)2850540.0483871DNA damage response
0.1958.85E-52SGTC_15258-methoxypsoralen 92.5 μMTimTec (Pure natural product library)41140.177419DNA damage response
0.1844.37E-46SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.0677966DNA damage response
0.1811.31E-44SGTC_503tyrphostin 8 294.0 μMICCB bioactive library929700.111111TSC3-RPN4
0.1781.73E-43SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.0659341RNA pol III & RNase P/MRP
0.1693.86E-39SGTC_1834st055981 52.8 μMTimTec (Natural product derivative library)4717190.266667RPP1 & pyrimidine depletion
0.1675.65E-38SGTC_1642st009582 77.7 μMTimTec (Natural product derivative library)67393110.125RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_26335,7-dihydroxyisoflavone100 μM0.6904765377381Microsource (Natural product library)254.23752.38224
SGTC_1929genistein trimethyl ether50.5 μM0.644444136420TimTec (Natural product derivative library)312.316642.81705
SGTC_2411st07713339.94 μM0.5576921403142TimTec (Natural product derivative library)368.336842.38507RNA pol III & RNase P/MRP
SGTC_2557derrustone83.84 μM0.472727629853Microsource (Natural product library)326.300162.60106
SGTC_2620irigenin100 μM0.4716985464170Microsource (Natural product library)360.314842.09138
SGTC_1854st05623332.9 μM0.4705885393174TimTec (Natural product derivative library)268.264083.1214amide catabolism
SGTC_2636apigenin dimethyl ether100 μM0.4423085281601Microsource (Natural product library)298.290062.86115
SGTC_2568diosmetin100 μM0.4181825281612Microsource (Natural product library)300.262882.39436
SGTC_1965Pectolinarigenin58.4 μM0.3928575320438TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_1115galangin695.68 nM0.3921575281616TimTec (Natural product library)270.23692.11435RPP1 & pyrimidine depletion