spermine

N,N'-bis(3-aminopropyl)butane-1,4-diamine

A DNA binding polyamine.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2523
Screen concentration 47.7 μM
Source Microsource (Natural product library)
PubChem CID 1103
SMILES C(CCNCCCN)CNCCCN
Standardized SMILES NCCCNCCCCNCCCN
Molecular weight 202.3402
ALogP -1.5
H-bond donor count 4
H-bond acceptor count 4
Response signature calcium & mitochondrial duress

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.1
% growth inhibition (Hom. pool) 3.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1103
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.22|P-value:6.49E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:FAS1(YKL182W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GLC7(YER133W)|FD-Score:3.79|P-value:7.63E-5|Clearance:0.31||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:RDS3(YPR094W)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.22||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:SEC15(YGL233W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.13||SGD DESC:Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Gene:TAF7(YMR227C)|FD-Score:4.36|P-value:6.43E-6|Clearance:0.58||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TOM40(YMR203W)|FD-Score:3.12|P-value:8.91E-4|Clearance:0.11||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YGR114C(YGR114C_d)|FD-Score:3.13|P-value:8.80E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:ACT1(YFL039C)|FD-Score:-3.22|P-value:6.49E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:FAS1(YKL182W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GLC7(YER133W)|FD-Score:3.79|P-value:7.63E-5|Clearance:0.31||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:RDS3(YPR094W)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.22||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:SEC15(YGL233W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.13||SGD DESC:Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Gene:TAF7(YMR227C)|FD-Score:4.36|P-value:6.43E-6|Clearance:0.58||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TOM40(YMR203W)|FD-Score:3.12|P-value:8.91E-4|Clearance:0.11||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YGR114C(YGR114C_d)|FD-Score:3.13|P-value:8.80E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1103
Download HOP data (tab-delimited text)  (excel)
Gene:ALD4(YOR374W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:APM2(YHL019C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:ARO1(YDR127W)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:5.25|P-value:7.63E-8||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG26(YLR189C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BCK1(YJL095W)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CYS4(YGR155W)|FD-Score:6.29|P-value:1.63E-10||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EMI1(YDR512C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG2(YMR202W)|FD-Score:21|P-value:4.63E-98||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FKH2(YNL068C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FMP37(YGL080W)|FD-Score:4.45|P-value:4.21E-6||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FRE5(YOR384W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUR4(YBR021W)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN2(YDR283C)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.62|P-value:9.42E-9||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLY1(YEL046C)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GND1(YHR183W)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GPB2(YAL056W)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSP2(YOR185C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAA1(YPR008W)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HMO1(YDR174W)|FD-Score:4.42|P-value:4.86E-6||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HST3(YOR025W)|FD-Score:4.77|P-value:9.32E-7||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:HXT17(YNR072W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:IDH2(YOR136W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IMP2'(YIL154C)|FD-Score:27.9|P-value:5.79E-172||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC13(YOR235W_d)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:LAA1(YJL207C)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LYS2(YBR115C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MIP1(YOR330C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MSK1(YNL073W)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:PCI8(YIL071C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PHM6(YDR281C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PHO4(YFR034C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO84(YML123C)|FD-Score:7.36|P-value:9.14E-14||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO86(YJL117W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PRO2(YOR323C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:REG1(YDR028C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGP1(YDR137W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPI1(YIL119C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPL39(YJL189W)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RPN4(YDL020C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:-4.74|P-value:1.05E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SSO1(YPL232W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SWM2(YNR004W_p)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TAT1(YBR069C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TPN1(YGL186C)|FD-Score:-6.41|P-value:7.25E-11||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM82(YDR165W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP1(YDR007W)|FD-Score:8.46|P-value:1.36E-17||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.1|P-value:5.14E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.92|P-value:2.27E-19||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.15|P-value:1.81E-16||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS65(YGR166W)|FD-Score:4.88|P-value:5.26E-7||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TUL1(YKL034W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBR1(YGR184C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:URA5(YML106W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS34(YLR240W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS45(YGL095C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS71(YML041C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR056W(YBR056W_p)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YDR008C(YDR008C_d)|FD-Score:8.87|P-value:3.81E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL015C(YFL015C_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YGL117W(YGL117W_p)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:-3.81|P-value:7.03E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHL044W(YHL044W_p)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIL054W(YIL054W_p)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL067C(YIL067C_p)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:-3.73|P-value:9.52E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YKL044W(YKL044W_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR419W(YLR419W_p)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR052C-A(YMR052C-A_d)|FD-Score:5.3|P-value:5.93E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:4.29|P-value:8.77E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL077C(YPL077C_p)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPR015C(YPR015C_p)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR053C(YPR053C_d)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YRF1-6(YNL339C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:YTA7(YGR270W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ZUO1(YGR285C)|FD-Score:7.36|P-value:9.46E-14||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:ALD4(YOR374W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:APM2(YHL019C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:ARO1(YDR127W)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:5.25|P-value:7.63E-8||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG26(YLR189C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BCK1(YJL095W)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CYS4(YGR155W)|FD-Score:6.29|P-value:1.63E-10||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EMI1(YDR512C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG2(YMR202W)|FD-Score:21|P-value:4.63E-98||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FKH2(YNL068C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FMP37(YGL080W)|FD-Score:4.45|P-value:4.21E-6||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FRE5(YOR384W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUR4(YBR021W)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN2(YDR283C)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.62|P-value:9.42E-9||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLY1(YEL046C)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GND1(YHR183W)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GPB2(YAL056W)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSP2(YOR185C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAA1(YPR008W)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HMO1(YDR174W)|FD-Score:4.42|P-value:4.86E-6||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HST3(YOR025W)|FD-Score:4.77|P-value:9.32E-7||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:HXT17(YNR072W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:IDH2(YOR136W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IMP2'(YIL154C)|FD-Score:27.9|P-value:5.79E-172||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC13(YOR235W_d)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:LAA1(YJL207C)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LYS2(YBR115C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MIP1(YOR330C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MSK1(YNL073W)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:PCI8(YIL071C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PHM6(YDR281C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PHO4(YFR034C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO84(YML123C)|FD-Score:7.36|P-value:9.14E-14||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO86(YJL117W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PRO2(YOR323C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:REG1(YDR028C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGP1(YDR137W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPI1(YIL119C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPL39(YJL189W)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RPN4(YDL020C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:-4.74|P-value:1.05E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SSO1(YPL232W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SWM2(YNR004W_p)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TAT1(YBR069C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TPN1(YGL186C)|FD-Score:-6.41|P-value:7.25E-11||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM82(YDR165W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP1(YDR007W)|FD-Score:8.46|P-value:1.36E-17||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.1|P-value:5.14E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.92|P-value:2.27E-19||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.15|P-value:1.81E-16||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS65(YGR166W)|FD-Score:4.88|P-value:5.26E-7||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TUL1(YKL034W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBR1(YGR184C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:URA5(YML106W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS34(YLR240W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS45(YGL095C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS71(YML041C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR056W(YBR056W_p)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YDR008C(YDR008C_d)|FD-Score:8.87|P-value:3.81E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL015C(YFL015C_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YGL117W(YGL117W_p)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:-3.81|P-value:7.03E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHL044W(YHL044W_p)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIL054W(YIL054W_p)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL067C(YIL067C_p)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:-3.73|P-value:9.52E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YKL044W(YKL044W_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR419W(YLR419W_p)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR052C-A(YMR052C-A_d)|FD-Score:5.3|P-value:5.93E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:4.29|P-value:8.77E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL077C(YPL077C_p)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPR015C(YPR015C_p)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR053C(YPR053C_d)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YRF1-6(YNL339C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:YTA7(YGR270W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ZUO1(YGR285C)|FD-Score:7.36|P-value:9.46E-14||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR227C4.366.43E-60.58TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YER133W3.797.63E-50.31GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YPR094W3.472.57E-40.22RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YGL233W3.255.69E-40.13SEC15Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
YGR114C_d3.138.80E-40.00YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YMR203W3.128.91E-40.11TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YPR041W3.020.001270.16TIF5Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2
YOL066C2.860.002110.06RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YMR131C2.810.002510.11RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YLR196W2.700.003490.10PWP1Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
YJR016C2.590.004740.05ILV3Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
YMR168C2.550.005420.04CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YIR012W2.510.006100.11SQT1Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
YMR314W2.400.008200.03PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YNL262W2.370.008960.01POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL154C27.905.79E-172IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YMR202W21.004.63E-98ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YKL211C8.922.27E-19TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR008C_d8.873.81E-19YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR007W8.461.36E-17TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR354W8.151.81E-16TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YML123C7.369.14E-14PHO84High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
YGR285C7.369.46E-14ZUO1Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
YGR155W6.291.63E-10CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YER090W6.105.14E-10TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YEL009C5.629.42E-9GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YIR023W5.491.98E-8DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YMR052C-A_d5.305.93E-8YMR052C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR035W5.257.63E-8ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YJL095W4.983.21E-7BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p

GO enrichment analysis for SGTC_2523
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4245.39E-257SGTC_400oxethazaine 37.5 μMMiscellaneous46210.0208333calcium & mitochondrial duress
0.3994.32E-224SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0227273ERG2
0.3922.35E-216SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.0727273calcium & mitochondrial duress
0.3901.00E-213SGTC_2981391-0729 10.0 μMChemDiv (Drug-like library)3237558NAcalcium & mitochondrial duress
0.3897.73E-213SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)16759247NA
0.3814.30E-203SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0188679ERG2
0.3701.46E-190SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)441302NA
0.3638.34E-184SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.0327869
0.3553.59E-175SGTC_6090986-0026 86.3 μMChemDiv (Drug-like library)2848610NAcalcium & mitochondrial duress
0.3555.26E-175SGTC_20384100038 31.0 μMChembridge (Fragment library)3596803NAplasma membrane duress
0.3504.35E-170SGTC_9943910-0338 138.0 μMChemDiv (Drug-like library)34794810.016129calcium & mitochondrial duress
0.3452.10E-164SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.0208333
0.3424.78E-162SGTC_330986-0033 35.8 μMChemDiv (Drug-like library)28480820.0188679calcium & mitochondrial duress
0.3419.42E-161SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0166667
0.3273.01E-147SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0555556

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_469w7172 μM0.31428645115570ICCB bioactive library290.830923.66622ERG2
SGTC_1573oleylamine1.06 μM0.2307695356789TimTec (Pure natural product library)267.493086.62311
SGTC_23549060492162 μM0.1538466471895Chembridge (Fragment library)201.26761.27823
SGTC_830170-030139.03 μM0.1515153089716ChemDiv (Drug-like library)1022.3524499.636236endomembrane recycling
SGTC_1564D-Arginine115 μM0.14285771070TimTec (Pure natural product library)174.20096-3.83144
SGTC_11343477-000942.5 μM0.1379313973534ChemDiv (Drug-like library)372.2166464.026212
SGTC_219mitoxanthrone164.41 μM0.1282054212Miscellaneous444.480920.068810DNA intercalators
SGTC_2763lisinopril49.32 μM0.1186445362119Miscellaneous405.48794-3.67347
SGTC_23809035439200 μM0.1176476462575Chembridge (Fragment library)201.693142.33922endomembrane recycling
SGTC_390dec-rvkr-cmk16.7 μM0.1129039962075Miscellaneous744.411541.88998calcium & mitochondrial duress