methyl deoxycholate

methyl 4-[(3R,5R,10S,12S,13R)-3,12-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoate

Haematoxylin.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2531
Screen concentration 70.1 μM
Source Microsource (Natural product library)
PubChem CID 16667744
SMILES CC(CCC(=O)OC)C1CCC2C1(C(CC3C2CCC4C3(CCC(C4)O)C)O)C
Standardized SMILES COC(=O)CCC(C)C1CCC2C3CCC4CC(O)CCC4(C)C3CC(O)C12C
Molecular weight 406.5986
ALogP 4.31
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.39
% growth inhibition (Hom. pool) 5.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16667744
Download HIP data (tab-delimited text)  (excel)
Gene:CDC11(YJR076C)|FD-Score:4.37|P-value:6.31E-6|Clearance:0.5||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC43(YGL155W)|FD-Score:-3.21|P-value:6.72E-4|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:GCD1(YOR260W)|FD-Score:4.82|P-value:7.28E-7|Clearance:0.14||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD2(YGR083C)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HSP10(YOR020C)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.03||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:KEG1(YFR042W)|FD-Score:-3.21|P-value:6.54E-4|Clearance:0||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MCM7(YBR202W)|FD-Score:3.87|P-value:5.44E-5|Clearance:0.07||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NAT2(YGR147C)|FD-Score:4.68|P-value:1.44E-6|Clearance:0.31||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PIK1(YNL267W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.11||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRP22(YER013W)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.18||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:PSF3(YOL146W)|FD-Score:3.15|P-value:8.15E-4|Clearance:0.03||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RIO2(YNL207W)|FD-Score:3.16|P-value:8.02E-4|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPB8(YOR224C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.13||SGD DESC:RNA polymerase III subunit C53 Gene:RPN11(YFR004W)|FD-Score:-4.56|P-value:2.51E-6|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RRP42(YDL111C)|FD-Score:-3.84|P-value:6.04E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RTT105(YER104W)|FD-Score:7.73|P-value:5.21E-15|Clearance:2.92||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SPC105(YGL093W)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:THS1(YIL078W)|FD-Score:3.8|P-value:7.37E-5|Clearance:0.12||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:UTP8(YGR128C)|FD-Score:-3.17|P-value:7.69E-4|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJR141W(YJR141W_p)|FD-Score:3.12|P-value:9.12E-4|Clearance:0.06||SGD DESC:Essential protein of unknown function Gene:YLR458W(YLR458W_d)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YNL150W(YNL150W_d)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:CDC11(YJR076C)|FD-Score:4.37|P-value:6.31E-6|Clearance:0.5||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC43(YGL155W)|FD-Score:-3.21|P-value:6.72E-4|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:GCD1(YOR260W)|FD-Score:4.82|P-value:7.28E-7|Clearance:0.14||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD2(YGR083C)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HSP10(YOR020C)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.03||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:KEG1(YFR042W)|FD-Score:-3.21|P-value:6.54E-4|Clearance:0||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MCM7(YBR202W)|FD-Score:3.87|P-value:5.44E-5|Clearance:0.07||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NAT2(YGR147C)|FD-Score:4.68|P-value:1.44E-6|Clearance:0.31||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PIK1(YNL267W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.11||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRP22(YER013W)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.18||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:PSF3(YOL146W)|FD-Score:3.15|P-value:8.15E-4|Clearance:0.03||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RIO2(YNL207W)|FD-Score:3.16|P-value:8.02E-4|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPB8(YOR224C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.13||SGD DESC:RNA polymerase III subunit C53 Gene:RPN11(YFR004W)|FD-Score:-4.56|P-value:2.51E-6|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RRP42(YDL111C)|FD-Score:-3.84|P-value:6.04E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RTT105(YER104W)|FD-Score:7.73|P-value:5.21E-15|Clearance:2.92||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SPC105(YGL093W)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:THS1(YIL078W)|FD-Score:3.8|P-value:7.37E-5|Clearance:0.12||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:UTP8(YGR128C)|FD-Score:-3.17|P-value:7.69E-4|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJR141W(YJR141W_p)|FD-Score:3.12|P-value:9.12E-4|Clearance:0.06||SGD DESC:Essential protein of unknown function Gene:YLR458W(YLR458W_d)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YNL150W(YNL150W_d)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16667744
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADE3(YGR204W)|FD-Score:5.82|P-value:2.96E-9||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADY3(YDL239C)|FD-Score:-4.51|P-value:3.26E-6||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:AFG1(YEL052W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:API2(YDR525W_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APP1(YNL094W)|FD-Score:-5.88|P-value:2.02E-9||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARP8(YOR141C)|FD-Score:5.29|P-value:6.04E-8||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG16(YMR159C)|FD-Score:6.53|P-value:3.30E-11||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP10(YLR393W)|FD-Score:-4.05|P-value:2.61E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCK1(YJL095W)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CIT1(YNR001C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CNM67(YNL225C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COQ5(YML110C)|FD-Score:-3.77|P-value:8.26E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CUP2(YGL166W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DAT1(YML113W)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DIG1(YPL049C)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOG2(YHR043C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DSE4(YNR067C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:EDE1(YBL047C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELP2(YGR200C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EOS1(YNL080C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FMP27(YLR454W_p)|FD-Score:-3.86|P-value:5.66E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV7(YLR068W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GDT1(YBR187W_p)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GLE2(YER107C)|FD-Score:6.56|P-value:2.69E-11||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GLG1(YKR058W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:-4.39|P-value:5.77E-6||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HAP5(YOR358W)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HOR7(YMR251W-A)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HTB2(YBL002W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IFM1(YOL023W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IME4(YGL192W)|FD-Score:-6.64|P-value:1.62E-11||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRA2(YOL081W)|FD-Score:5.18|P-value:1.08E-7||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:LOT5(YKL183W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MDV1(YJL112W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MFB1(YDR219C)|FD-Score:-3.91|P-value:4.68E-5||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MRP20(YDR405W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTC6(YHR151C)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NPC2(YDL046W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OST6(YML019W)|FD-Score:-4|P-value:3.23E-5||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PAN3(YKL025C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PBP1(YGR178C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:7.16|P-value:4.14E-13||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX14(YGL153W)|FD-Score:-4.58|P-value:2.37E-6||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PHO80(YOL001W)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PMT3(YOR321W)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PST1(YDR055W)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PTC5(YOR090C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:QCR8(YJL166W)|FD-Score:-4.03|P-value:2.84E-5||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:RBK1(YCR036W)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative ribokinase Gene:RGP1(YDR137W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM5(YLR137W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RNH203(YLR154C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPL22B(YFL034C-A)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS27B(YHR021C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTN1(YDR233C)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:6.84|P-value:4.08E-12||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAK1(YER129W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SCW10(YMR305C)|FD-Score:-13.9|P-value:4.20E-44||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SIN4(YNL236W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIT4(YDL047W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLO1(YER180C-A)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SLT2(YHR030C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNT2(YGL131C)|FD-Score:-4.28|P-value:9.22E-6||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SOP4(YJL192C)|FD-Score:-4.59|P-value:2.17E-6||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO7(YAL009W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSD1(YDR293C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:6.87|P-value:3.26E-12||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SUS1(YBR111W-A)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TIM21(YGR033C)|FD-Score:-3.74|P-value:9.36E-5||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TPM1(YNL079C)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA4(YHR111W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UGA1(YGR019W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress Gene:UGA3(YDL170W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VAC17(YCL063W)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VID28(YIL017C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPH1(YOR270C)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS29(YHR012W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS55(YJR044C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS71(YML041C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:WWM1(YFL010C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:YAP1802(YGR241C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YAT2(YER024W)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane Gene:YCR022C(YCR022C_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YCR090C(YCR090C_p)|FD-Score:4.81|P-value:7.68E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL180W(YDL180W_p)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YEL028W(YEL028W_d)|FD-Score:-4.38|P-value:5.87E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER152C(YER152C)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YFL019C(YFL019C_d)|FD-Score:-5.05|P-value:2.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR045C(YGR045C_d)|FD-Score:-4.78|P-value:8.57E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:-5.18|P-value:1.11E-7||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJL028W(YJL028W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments Gene:YJL055W(YJL055W_p)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YLR287C(YLR287C_p)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML009C-A(YML009C-A_d)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR141C(YMR141C_d)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR209C(YMR209C_p)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL095C(YNL095C_p)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YOL153C(YOL153C)|FD-Score:-4.8|P-value:7.98E-7||SGD DESC:Hypothetical protein Gene:YOR008C-A(YOR008C-A_p)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR225W(YOR225W_d)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT31(YER031C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YVH1(YIR026C)|FD-Score:4.3|P-value:8.58E-6||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ACE2(YLR131C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADE3(YGR204W)|FD-Score:5.82|P-value:2.96E-9||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADY3(YDL239C)|FD-Score:-4.51|P-value:3.26E-6||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:AFG1(YEL052W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:API2(YDR525W_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APP1(YNL094W)|FD-Score:-5.88|P-value:2.02E-9||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARP8(YOR141C)|FD-Score:5.29|P-value:6.04E-8||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG16(YMR159C)|FD-Score:6.53|P-value:3.30E-11||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP10(YLR393W)|FD-Score:-4.05|P-value:2.61E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCK1(YJL095W)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CIT1(YNR001C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CNM67(YNL225C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COQ5(YML110C)|FD-Score:-3.77|P-value:8.26E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CUP2(YGL166W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DAT1(YML113W)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DIG1(YPL049C)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOG2(YHR043C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DSE4(YNR067C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:EDE1(YBL047C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELP2(YGR200C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EOS1(YNL080C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FMP27(YLR454W_p)|FD-Score:-3.86|P-value:5.66E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV7(YLR068W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GDT1(YBR187W_p)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GLE2(YER107C)|FD-Score:6.56|P-value:2.69E-11||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GLG1(YKR058W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:-4.39|P-value:5.77E-6||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HAP5(YOR358W)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HOR7(YMR251W-A)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HTB2(YBL002W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IFM1(YOL023W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IME4(YGL192W)|FD-Score:-6.64|P-value:1.62E-11||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRA2(YOL081W)|FD-Score:5.18|P-value:1.08E-7||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:LOT5(YKL183W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MDV1(YJL112W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MFB1(YDR219C)|FD-Score:-3.91|P-value:4.68E-5||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MRP20(YDR405W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTC6(YHR151C)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NPC2(YDL046W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OST6(YML019W)|FD-Score:-4|P-value:3.23E-5||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PAN3(YKL025C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PBP1(YGR178C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:7.16|P-value:4.14E-13||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX14(YGL153W)|FD-Score:-4.58|P-value:2.37E-6||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PHO80(YOL001W)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PMT3(YOR321W)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PST1(YDR055W)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PTC5(YOR090C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:QCR8(YJL166W)|FD-Score:-4.03|P-value:2.84E-5||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:RBK1(YCR036W)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative ribokinase Gene:RGP1(YDR137W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM5(YLR137W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RNH203(YLR154C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPL22B(YFL034C-A)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS27B(YHR021C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTN1(YDR233C)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:6.84|P-value:4.08E-12||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAK1(YER129W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SCW10(YMR305C)|FD-Score:-13.9|P-value:4.20E-44||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SIN4(YNL236W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIT4(YDL047W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLO1(YER180C-A)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SLT2(YHR030C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNT2(YGL131C)|FD-Score:-4.28|P-value:9.22E-6||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SOP4(YJL192C)|FD-Score:-4.59|P-value:2.17E-6||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO7(YAL009W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSD1(YDR293C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:6.87|P-value:3.26E-12||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SUS1(YBR111W-A)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TIM21(YGR033C)|FD-Score:-3.74|P-value:9.36E-5||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TPM1(YNL079C)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA4(YHR111W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UGA1(YGR019W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress Gene:UGA3(YDL170W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VAC17(YCL063W)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VID28(YIL017C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPH1(YOR270C)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS29(YHR012W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS55(YJR044C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS71(YML041C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:WWM1(YFL010C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:YAP1802(YGR241C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YAT2(YER024W)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane Gene:YCR022C(YCR022C_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YCR090C(YCR090C_p)|FD-Score:4.81|P-value:7.68E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL180W(YDL180W_p)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YEL028W(YEL028W_d)|FD-Score:-4.38|P-value:5.87E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER152C(YER152C)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YFL019C(YFL019C_d)|FD-Score:-5.05|P-value:2.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR045C(YGR045C_d)|FD-Score:-4.78|P-value:8.57E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:-5.18|P-value:1.11E-7||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJL028W(YJL028W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments Gene:YJL055W(YJL055W_p)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YLR287C(YLR287C_p)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML009C-A(YML009C-A_d)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR141C(YMR141C_d)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR209C(YMR209C_p)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL095C(YNL095C_p)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YOL153C(YOL153C)|FD-Score:-4.8|P-value:7.98E-7||SGD DESC:Hypothetical protein Gene:YOR008C-A(YOR008C-A_p)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR225W(YOR225W_d)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT31(YER031C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YVH1(YIR026C)|FD-Score:4.3|P-value:8.58E-6||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER104W7.735.21E-152.92RTT105Protein with a role in regulation of Ty1 transposition
YOR260W4.827.28E-70.14GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YGR147C4.681.44E-60.31NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YJR076C4.376.31E-60.50CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YBR202W3.875.44E-50.07MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YIL078W3.807.37E-50.12THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YNL150W_d3.671.20E-40.01YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YLR458W_d3.671.24E-40.06YLR458W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis
YOR020C3.601.58E-40.03HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YNL267W3.571.79E-40.11PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YER013W3.462.69E-40.18PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YDL150W3.285.14E-40.13RPC53RNA polymerase III subunit C53
YNL207W3.168.02E-40.00RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YOL146W3.158.15E-40.03PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YJR141W_p3.129.12E-40.06YJR141W_pEssential protein of unknown function

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YGL013C7.164.14E-13PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YLR372W6.873.26E-12SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YJL047C6.844.08E-12RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YER107C6.562.69E-11GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YMR159C6.533.30E-11ATG16Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YGR204W5.822.96E-9ADE3Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
YHR151C5.551.39E-8MTC6Protein of unknown function; mtc6 is synthetically sick with cdc13-1
YOR141C5.296.04E-8ARP8Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity
YJL095W5.201.01E-7BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YOL081W5.181.08E-7IRA2GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication
YDR293C4.963.51E-7SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YCR090C_p4.817.68E-7YCR090C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene
YER129W4.751.03E-6SAK1Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
YLR068W4.741.05E-6FYV7Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
YER152C4.405.49E-6YER152CProtein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene

GO enrichment analysis for SGTC_2531
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1412.07E-27SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0560748PDR1
0.1194.41E-20SGTC_2740felodipine 17.1 μMMiscellaneous33330.103448
0.1132.74E-18SGTC_5481436-0017 110.0 μMChemDiv (Drug-like library)96771370.0744681TSC3-RPN4
0.1125.82E-18SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.103093PDR1
0.1102.25E-17SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.0408163PDR1
0.1093.37E-17SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0540541PDR1
0.1095.65E-17SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.0454545PDR1
0.1072.09E-16SGTC_483fpl-64176 144.0 μMICCB bioactive library34230.0952381cell wall
0.1063.91E-16SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.119048PDR1
0.1041.30E-15SGTC_14103909-8106 77.9 μMChemDiv (Drug-like library)10392100.050505
0.1001.03E-14SGTC_4253825-7017 13.1 μMChemDiv (Drug-like library)33974400.0107527
0.1001.19E-14SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0679612PDR1
0.0976.05E-14SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0875TSC3-RPN4
0.0977.77E-14SGTC_31189124250 49.5 μMChembridge (Drug-like library)15047860.0337079
0.0961.92E-13SGTC_422cantharidin 155.0 μMICCB bioactive library25450.104478cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1517deoxycholic acid methyl ester49.2 μM1229346TimTec (Pure natural product library)406.598584.30724
SGTC_2555cholic acid100 μM0.7540986708657Microsource (Natural product library)422.597983.13835
SGTC_1560lithocholic acid53.1 μM0.6229519903TimTec (Pure natural product library)376.57265.18423
SGTC_2621lithochol-11-enic acid100 μM0.5142866708711Microsource (Natural product library)374.556724.73923
SGTC_2687chenodiol100 μM0.4925375702181Microsource (Natural product library)392.5724.01434
SGTC_1572epiandrosterone68.9 μM0.378788441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.378788441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.3787882754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_2628lathosterol100 μM0.3333336710616Microsource (Natural product library)386.653547.37611
SGTC_1974st06944826.9 μM0.281696323587TimTec (Natural product derivative library)305.454943.36923
SGTC_1750st0818592.36 μM0.2537315291695TimTec (Natural product derivative library)275.472024.43511endomembrane recycling