helenine

(3aR,5S,8aR,9aR)-5,8a-dimethyl-3-methylidene-5,6,7,8,9,9a-hexahydro-3aH-benzo[f][1]benzofuran-2-one

Natural product isolated from Penicillium funiculosum with antiviral properties.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2534
Screen concentration 20.1 μM
Source Microsource (Natural product library)
PubChem CID 72724
SMILES CC1CCCC2(C1=CC3C(C2)OC(=O)C3=C)C
Standardized SMILES CC1CCCC2(C)CC3OC(=O)C(=C)C3C=C12
Molecular weight 232.3181
ALogP 3.31
H-bond donor count 0
H-bond acceptor count 2
Response signature RSC complex & mRNA processing

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.51
% growth inhibition (Hom. pool) 4.51


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 72724
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:6.16|P-value:3.55E-10|Clearance:0.35||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:4.08|P-value:2.21E-5|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:5.81|P-value:3.11E-9|Clearance:0.12||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:4.45|P-value:4.34E-6|Clearance:0.36||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT5(YJR064W)|FD-Score:-3.1|P-value:9.67E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-4.22|P-value:1.22E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC20(YGL116W)|FD-Score:-4.33|P-value:7.62E-6|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CRM1(YGR218W)|FD-Score:4.08|P-value:2.23E-5|Clearance:0.06||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:FHL1(YPR104C)|FD-Score:3.22|P-value:6.36E-4|Clearance:0.15||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:GPN2(YOR262W)|FD-Score:5.69|P-value:6.49E-9|Clearance:0.18||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HYP2(YEL034W)|FD-Score:4.02|P-value:2.88E-5|Clearance:0.05||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSM2(YBL026W)|FD-Score:5.17|P-value:1.18E-7|Clearance:0.72||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MED4(YOR174W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.06||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAT2(YGR147C)|FD-Score:-3.66|P-value:1.29E-4|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NFS1(YCL017C)|FD-Score:3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NIP7(YPL211W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NOG2(YNR053C)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NSE3(YDR288W)|FD-Score:-7.55|P-value:2.19E-14|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP192(YJL039C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.02||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PAB1(YER165W)|FD-Score:7.15|P-value:4.32E-13|Clearance:0.06||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PKC1(YBL105C)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.1||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RBA50(YDR527W)|FD-Score:6.37|P-value:9.38E-11|Clearance:0.21||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RLP24(YLR009W)|FD-Score:-3.19|P-value:7.17E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB10(YOR210W)|FD-Score:7.55|P-value:2.16E-14|Clearance:0.22||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB11(YOL005C)|FD-Score:3.79|P-value:7.65E-5|Clearance:0.14||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPB8(YOR224C)|FD-Score:-4.12|P-value:1.88E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL32(YBL092W)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPP1(YHR062C)|FD-Score:3.9|P-value:4.78E-5|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS2(YGL123W)|FD-Score:-4.06|P-value:2.47E-5|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RSC58(YLR033W)|FD-Score:22.5|P-value:5.00E-112|Clearance:9.56||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:12.9|P-value:2.24E-38|Clearance:0.48||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:10.4|P-value:1.17E-25|Clearance:1.56||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RTP1(YMR185W_p)|FD-Score:7.33|P-value:1.15E-13|Clearance:0.18||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SFH1(YLR321C)|FD-Score:5.51|P-value:1.79E-8|Clearance:0.34||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SPB4(YFL002C)|FD-Score:7.09|P-value:6.50E-13|Clearance:0.72||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC105(YGL093W)|FD-Score:7.87|P-value:1.80E-15|Clearance:0.32||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TFC3(YAL001C)|FD-Score:8.85|P-value:4.56E-19|Clearance:0.98||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIF35(YDR429C)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YDR187C(YDR187C_d)|FD-Score:-4.12|P-value:1.91E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGL069C(YGL069C_d)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.07||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YRB1(YDR002W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.12||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:YTH1(YPR107C)|FD-Score:12.4|P-value:9.63E-36|Clearance:2.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:6.16|P-value:3.55E-10|Clearance:0.35||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:4.08|P-value:2.21E-5|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:5.81|P-value:3.11E-9|Clearance:0.12||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:4.45|P-value:4.34E-6|Clearance:0.36||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT5(YJR064W)|FD-Score:-3.1|P-value:9.67E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-4.22|P-value:1.22E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC20(YGL116W)|FD-Score:-4.33|P-value:7.62E-6|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CRM1(YGR218W)|FD-Score:4.08|P-value:2.23E-5|Clearance:0.06||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:FHL1(YPR104C)|FD-Score:3.22|P-value:6.36E-4|Clearance:0.15||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:GPN2(YOR262W)|FD-Score:5.69|P-value:6.49E-9|Clearance:0.18||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HYP2(YEL034W)|FD-Score:4.02|P-value:2.88E-5|Clearance:0.05||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSM2(YBL026W)|FD-Score:5.17|P-value:1.18E-7|Clearance:0.72||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MED4(YOR174W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.06||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAT2(YGR147C)|FD-Score:-3.66|P-value:1.29E-4|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NFS1(YCL017C)|FD-Score:3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NIP7(YPL211W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NOG2(YNR053C)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NSE3(YDR288W)|FD-Score:-7.55|P-value:2.19E-14|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP192(YJL039C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.02||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PAB1(YER165W)|FD-Score:7.15|P-value:4.32E-13|Clearance:0.06||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PKC1(YBL105C)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.1||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RBA50(YDR527W)|FD-Score:6.37|P-value:9.38E-11|Clearance:0.21||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RLP24(YLR009W)|FD-Score:-3.19|P-value:7.17E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB10(YOR210W)|FD-Score:7.55|P-value:2.16E-14|Clearance:0.22||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB11(YOL005C)|FD-Score:3.79|P-value:7.65E-5|Clearance:0.14||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPB8(YOR224C)|FD-Score:-4.12|P-value:1.88E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL32(YBL092W)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPP1(YHR062C)|FD-Score:3.9|P-value:4.78E-5|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS2(YGL123W)|FD-Score:-4.06|P-value:2.47E-5|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RSC58(YLR033W)|FD-Score:22.5|P-value:5.00E-112|Clearance:9.56||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:12.9|P-value:2.24E-38|Clearance:0.48||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:10.4|P-value:1.17E-25|Clearance:1.56||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RTP1(YMR185W_p)|FD-Score:7.33|P-value:1.15E-13|Clearance:0.18||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SFH1(YLR321C)|FD-Score:5.51|P-value:1.79E-8|Clearance:0.34||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SPB4(YFL002C)|FD-Score:7.09|P-value:6.50E-13|Clearance:0.72||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC105(YGL093W)|FD-Score:7.87|P-value:1.80E-15|Clearance:0.32||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TFC3(YAL001C)|FD-Score:8.85|P-value:4.56E-19|Clearance:0.98||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIF35(YDR429C)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YDR187C(YDR187C_d)|FD-Score:-4.12|P-value:1.91E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGL069C(YGL069C_d)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.07||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YRB1(YDR002W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.12||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:YTH1(YPR107C)|FD-Score:12.4|P-value:9.63E-36|Clearance:2.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 72724
Download HOP data (tab-delimited text)  (excel)
Gene:APT1(YML022W)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication Gene:ARP6(YLR085C)|FD-Score:6.16|P-value:3.69E-10||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ASF1(YJL115W)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATG16(YMR159C)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BTS1(YPL069C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAN1(YEL063C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CLB5(YPR120C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COY1(YKL179C)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:DID4(YKL002W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DSS1(YMR287C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM18(YDR125C)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:GCN4(YEL009C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HTZ1(YOL012C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ICL2(YPR006C)|FD-Score:5.37|P-value:3.88E-8||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:IDP1(YDL066W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:ILM1(YJR118C)|FD-Score:5.28|P-value:6.45E-8||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IRC9(YJL142C_d)|FD-Score:-5.35|P-value:4.38E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IST2(YBR086C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:IZH1(YDR492W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:MBA1(YBR185C)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MFB1(YDR219C)|FD-Score:-3.82|P-value:6.56E-5||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MMR1(YLR190W)|FD-Score:-5.91|P-value:1.74E-9||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MTC4(YBR255W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MTH1(YDR277C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:NAS2(YIL007C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:NTO1(YPR031W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OST6(YML019W)|FD-Score:4.3|P-value:8.44E-6||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PBS2(YJL128C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PGD1(YGL025C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PLP1(YDR183W)|FD-Score:-6.58|P-value:2.41E-11||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:POM34(YLR018C)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication Gene:QCR8(YJL166W)|FD-Score:4.96|P-value:3.56E-7||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:REX2(YLR059C)|FD-Score:-3.74|P-value:9.33E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RGD2(YFL047W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RRT8(YOL048C_p)|FD-Score:-3.83|P-value:6.44E-5||SGD DESC:Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress Gene:RSM18(YER050C)|FD-Score:-3.83|P-value:6.33E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTC2(YBR147W)|FD-Score:-4.67|P-value:1.52E-6||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:SGT2(YOR007C)|FD-Score:5.21|P-value:9.66E-8||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SIZ1(YDR409W)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SUS1(YBR111W-A)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWC5(YBR231C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TPN1(YGL186C)|FD-Score:9|P-value:1.11E-19||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSC3(YBR058C-A)|FD-Score:5.93|P-value:1.51E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:10.6|P-value:1.54E-26||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS71(YML041C)|FD-Score:7.21|P-value:2.85E-13||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCR022C(YCR022C_d)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YDL009C(YDL009C_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR290W(YDR290W_d)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YGK3(YOL128C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGR021W(YGR021W_p)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YIL161W(YIL161W_p)|FD-Score:-4.49|P-value:3.56E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YOR024W(YOR024W_d)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL114W(YPL114W_d)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:APT1(YML022W)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication Gene:ARP6(YLR085C)|FD-Score:6.16|P-value:3.69E-10||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ASF1(YJL115W)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATG16(YMR159C)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BTS1(YPL069C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAN1(YEL063C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CLB5(YPR120C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COY1(YKL179C)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:DID4(YKL002W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DSS1(YMR287C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM18(YDR125C)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:GCN4(YEL009C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HTZ1(YOL012C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ICL2(YPR006C)|FD-Score:5.37|P-value:3.88E-8||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:IDP1(YDL066W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:ILM1(YJR118C)|FD-Score:5.28|P-value:6.45E-8||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IRC9(YJL142C_d)|FD-Score:-5.35|P-value:4.38E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IST2(YBR086C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:IZH1(YDR492W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:MBA1(YBR185C)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MFB1(YDR219C)|FD-Score:-3.82|P-value:6.56E-5||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MMR1(YLR190W)|FD-Score:-5.91|P-value:1.74E-9||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MTC4(YBR255W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MTH1(YDR277C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:NAS2(YIL007C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:NTO1(YPR031W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OST6(YML019W)|FD-Score:4.3|P-value:8.44E-6||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PBS2(YJL128C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PGD1(YGL025C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PLP1(YDR183W)|FD-Score:-6.58|P-value:2.41E-11||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:POM34(YLR018C)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication Gene:QCR8(YJL166W)|FD-Score:4.96|P-value:3.56E-7||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:REX2(YLR059C)|FD-Score:-3.74|P-value:9.33E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RGD2(YFL047W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RRT8(YOL048C_p)|FD-Score:-3.83|P-value:6.44E-5||SGD DESC:Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress Gene:RSM18(YER050C)|FD-Score:-3.83|P-value:6.33E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTC2(YBR147W)|FD-Score:-4.67|P-value:1.52E-6||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:SGT2(YOR007C)|FD-Score:5.21|P-value:9.66E-8||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SIZ1(YDR409W)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SUS1(YBR111W-A)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWC5(YBR231C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TPN1(YGL186C)|FD-Score:9|P-value:1.11E-19||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSC3(YBR058C-A)|FD-Score:5.93|P-value:1.51E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:10.6|P-value:1.54E-26||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS71(YML041C)|FD-Score:7.21|P-value:2.85E-13||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCR022C(YCR022C_d)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YDL009C(YDL009C_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR290W(YDR290W_d)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YGK3(YOL128C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGR021W(YGR021W_p)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YIL161W(YIL161W_p)|FD-Score:-4.49|P-value:3.56E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YOR024W(YOR024W_d)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL114W(YPL114W_d)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W22.505.00E-1129.56RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YFR037C12.902.24E-380.48RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YPR107C12.409.63E-362.02YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YML127W10.401.17E-251.56RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YAL001C8.854.56E-190.98TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YGL093W7.871.80E-150.32SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YOR210W7.552.16E-140.22RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YMR185W_p7.331.15E-130.18RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YER165W7.154.32E-130.06PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YFL002C7.096.50E-130.72SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YDR527W6.379.38E-110.21RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YNR016C6.163.55E-100.35ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YPR034W5.813.11E-90.12ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOR262W5.696.49E-90.17GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YLR321C5.511.79E-80.34SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML124C10.601.54E-26TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YGL186C9.001.11E-19TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YML041C7.212.85E-13VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YLR085C6.163.69E-10ARP6Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YBR058C-A5.931.51E-9TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YPR006C5.373.88E-8ICL22-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol
YJR118C5.286.45E-8ILM1Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
YOR007C5.219.66E-8SGT2Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
YJL166W4.963.56E-7QCR8Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
YML019W4.308.44E-6OST6Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
YNL325C4.241.12E-5FIG4Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain
YOL012C4.171.52E-5HTZ1Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
YPL069C4.112.00E-5BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YPR031W4.062.47E-5NTO1Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3
YCL063W4.013.09E-5VAC17Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p

GO enrichment analysis for SGTC_2534
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5340SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0833333RSC complex & mRNA processing
0.4700SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.0810811RSC complex & mRNA processing
0.4364.21E-273SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.112676RSC complex & mRNA processing
0.4244.34E-257SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.101695RSC complex & mRNA processing
0.4233.34E-255SGTC_7093807-1509 243.0 μMChemDiv (Drug-like library)18866770.0540541RSC complex & mRNA processing
0.4001.67E-225SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0606061
0.3995.52E-224SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0606061RSC complex & mRNA processing
0.3893.88E-212SGTC_6731082-0474 110.0 μMChemDiv (Drug-like library)30980460.0724638RSC complex & mRNA processing
0.3825.97E-204SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0394737RSC & ERG11
0.3714.37E-192SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0517241
0.3629.26E-183SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.0483871RSC complex & mRNA processing
0.3591.77E-179SGTC_12290139-0193 12.2 μMChemDiv (Drug-like library)30907520.0169492RSC complex & mRNA processing
0.3575.88E-177SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.0833333RSC complex & mRNA processing
0.3533.63E-173SGTC_8710574-0004 149.0 μMChemDiv (Drug-like library)95511730.0533333
0.3514.66E-171SGTC_14934348-0038 111.0 μMChemDiv (Drug-like library)8004240.0273973RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1119parthenolide318.54 nM0.2903235702252TimTec (Natural product library)248.31752.92303TSC3-RPN4
SGTC_25383',7'-epoxycaryophyllan-6-one100 μM0.2068974330530Microsource (Natural product library)236.349862.59902
SGTC_2558carylophyllene oxide100 μM0.2068976708694Microsource (Natural product library)206.323883.06301
SGTC_2519cryptotanshinone88.04 μM0.205882160254Microsource (Natural product library)296.36033.76103heme biosynthesis & mitochondrial translocase
SGTC_1125tnp002481.33 μM0.2033924207736TimTec (Natural product library)250.376443.49502
SGTC_2673kobusone100 μM0.203396710676Microsource (Natural product library)222.323282.25102
SGTC_1113artemisinin1.18 μM0.2031252240TimTec (Natural product library)282.332181.99805heme biosynthesis & mitochondrial translocase
SGTC_2415hr heat shock (37°C) + cantharidin155.45 μM0.22545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_2489cantharidin73.42 μM0.22545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_408cantharidin100 μM0.22545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_422cantharidin155 μM0.22545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_1772st04843982.2 μM0.1935482927068TimTec (Natural product derivative library)243.300982.72702