dehydroabietamide

1,4a-dimethyl-7-propan-2-yl-2,3,4,9,10,10a-hexahydrophenanthrene-1-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2536
Screen concentration 32.0 μM
Source Microsource (Natural product library)
PubChem CID 3983547
SMILES CC(C)C1=CC2=C(C=C1)C3(CCCC(C3CC2)(C)C(=O)N)C
Standardized SMILES CC(C)c1ccc2c(CCC3C(C)(CCCC23C)C(=O)N)c1
Molecular weight 299.4504
ALogP 4.76
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.12
% growth inhibition (Hom. pool) 6.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3983547
Download HIP data (tab-delimited text)  (excel)
Gene:BRN1(YBL097W)|FD-Score:-6.36|P-value:1.03E-10|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC60(YPL160W)|FD-Score:5.33|P-value:4.80E-8|Clearance:0.51||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:CWC2(YDL209C)|FD-Score:-4.82|P-value:7.23E-7|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:IMP4(YNL075W)|FD-Score:3.18|P-value:7.41E-4|Clearance:0.31||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:NAT2(YGR147C)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.56||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOG2(YNR053C)|FD-Score:6.15|P-value:3.80E-10|Clearance:0.59||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:PMI40(YER003C)|FD-Score:3.9|P-value:4.81E-5|Clearance:0.31||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RER2(YBR002C)|FD-Score:-3.34|P-value:4.25E-4|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFT1(YBL020W)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RHO1(YPR165W)|FD-Score:5.56|P-value:1.36E-8|Clearance:0.22||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPS2(YGL123W)|FD-Score:-3.28|P-value:5.17E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RRN6(YBL014C)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.17||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SFT1(YKL006C-A)|FD-Score:-4.21|P-value:1.27E-5|Clearance:0||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:TIM17(YJL143W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.32||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM50(YPL063W)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.1||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:USO1(YDL058W)|FD-Score:4.82|P-value:7.04E-7|Clearance:0.2||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:BRN1(YBL097W)|FD-Score:-6.36|P-value:1.03E-10|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC60(YPL160W)|FD-Score:5.33|P-value:4.80E-8|Clearance:0.51||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:CWC2(YDL209C)|FD-Score:-4.82|P-value:7.23E-7|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:IMP4(YNL075W)|FD-Score:3.18|P-value:7.41E-4|Clearance:0.31||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:NAT2(YGR147C)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.56||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOG2(YNR053C)|FD-Score:6.15|P-value:3.80E-10|Clearance:0.59||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:PMI40(YER003C)|FD-Score:3.9|P-value:4.81E-5|Clearance:0.31||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RER2(YBR002C)|FD-Score:-3.34|P-value:4.25E-4|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFT1(YBL020W)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RHO1(YPR165W)|FD-Score:5.56|P-value:1.36E-8|Clearance:0.22||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPS2(YGL123W)|FD-Score:-3.28|P-value:5.17E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RRN6(YBL014C)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.17||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SFT1(YKL006C-A)|FD-Score:-4.21|P-value:1.27E-5|Clearance:0||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:TIM17(YJL143W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.32||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM50(YPL063W)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.1||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:USO1(YDL058W)|FD-Score:4.82|P-value:7.04E-7|Clearance:0.2||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3983547
Download HOP data (tab-delimited text)  (excel)
Gene:ADH6(YMR318C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AIM43(YPL099C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ATP10(YLR393W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCK1(YJL095W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BFR1(YOR198C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BNA4(YBL098W)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:BUD19(YJL188C_d)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CBP6(YBR120C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CHS7(YHR142W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COQ1(YBR003W)|FD-Score:-3.97|P-value:3.65E-5||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:COR1(YBL045C)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:CTK1(YKL139W)|FD-Score:4.35|P-value:6.90E-6||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DEG1(YFL001W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DOA1(YKL213C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DPH5(YLR172C)|FD-Score:5.77|P-value:3.97E-9||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:ECM34(YHL043W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:FIR1(YER032W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FYV7(YLR068W)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:FYV8(YGR196C)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GEA2(YEL022W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GLY1(YEL046C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HCR1(YLR192C)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HCS1(YKL017C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:ISA1(YLL027W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:KEL2(YGR238C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KNS1(YLL019C)|FD-Score:4.45|P-value:4.34E-6||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LIP5(YOR196C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MBP1(YDL056W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MET18(YIL128W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MKT1(YNL085W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MNN10(YDR245W)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MNN11(YJL183W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPL15(YLR312W-A)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH5(YDL154W)|FD-Score:6.51|P-value:3.74E-11||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MSW1(YDR268W)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NCR1(YPL006W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NEJ1(YLR265C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:NIF3(YGL221C)|FD-Score:4.57|P-value:2.40E-6||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PAF1(YBR279W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PBP1(YGR178C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PDH1(YPR002W)|FD-Score:4.97|P-value:3.29E-7||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PEP8(YJL053W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PIR3(YKL163W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:POA1(YBR022W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PSR1(YLL010C)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUF3(YLL013C)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:QCR6(YFR033C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RIM101(YHL027W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:ROM2(YLR371W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL22B(YFL034C-A)|FD-Score:-4.65|P-value:1.69E-6||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPL23B(YER117W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPS11A(YDR025W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RTT103(YDR289C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RTT109(YLL002W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SAC6(YDR129C)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAK1(YER129W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SAM1(YLR180W)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SAP30(YMR263W)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SIP5(YMR140W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SLT2(YHR030C)|FD-Score:4.35|P-value:6.72E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF3(YDL194W)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNO1(YMR095C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SPO11(YHL022C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPT7(YBR081C)|FD-Score:4.34|P-value:7.25E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:STO1(YMR125W)|FD-Score:5.28|P-value:6.45E-8||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUB1(YMR039C)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SYF2(YGR129W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:THI6(YPL214C)|FD-Score:4.9|P-value:4.91E-7||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:THP1(YOL072W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TPM1(YNL079C)|FD-Score:4.91|P-value:4.46E-7||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:UGA3(YDL170W)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS35(YJL154C)|FD-Score:4.53|P-value:2.92E-6||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS55(YJR044C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS61(YDR136C_d)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR232C(YBR232C_d)|FD-Score:4.27|P-value:9.99E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR061W(YCR061W_p)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDL094C(YDL094C_d)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR521W(YDR521W_d)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YEN1(YER041W)|FD-Score:6.09|P-value:5.48E-10||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFL019C(YFL019C_d)|FD-Score:6.19|P-value:3.07E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGK3(YOL128C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL010W(YGL010W_p)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR045C(YGR045C_d)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:-6.09|P-value:5.75E-10||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHI9(YHR029C)|FD-Score:-3.76|P-value:8.46E-5||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YIR024C(YIR024C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YKL222C(YKL222C)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR001C(YLR001C_p)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR184W(YLR184W_d)|FD-Score:7.05|P-value:8.80E-13||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR202C(YLR202C_d)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR278C(YLR278C_p)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YLR287C(YLR287C_p)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YLR455W(YLR455W_p)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR166C(YMR166C_p)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR294W-A(YMR294W-A_d)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL108C(YNL108C_p)|FD-Score:-4.51|P-value:3.31E-6||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOL036W(YOL036W_p)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL025C(YPL025C_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:ADH6(YMR318C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AIM43(YPL099C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ATP10(YLR393W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCK1(YJL095W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BFR1(YOR198C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BNA4(YBL098W)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:BUD19(YJL188C_d)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CBP6(YBR120C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CHS7(YHR142W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COQ1(YBR003W)|FD-Score:-3.97|P-value:3.65E-5||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:COR1(YBL045C)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:CTK1(YKL139W)|FD-Score:4.35|P-value:6.90E-6||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DEG1(YFL001W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DOA1(YKL213C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DPH5(YLR172C)|FD-Score:5.77|P-value:3.97E-9||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:ECM34(YHL043W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:FIR1(YER032W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FYV7(YLR068W)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:FYV8(YGR196C)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GEA2(YEL022W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GLY1(YEL046C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HCR1(YLR192C)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HCS1(YKL017C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:ISA1(YLL027W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:KEL2(YGR238C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KNS1(YLL019C)|FD-Score:4.45|P-value:4.34E-6||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LIP5(YOR196C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MBP1(YDL056W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MET18(YIL128W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MKT1(YNL085W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MNN10(YDR245W)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MNN11(YJL183W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPL15(YLR312W-A)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH5(YDL154W)|FD-Score:6.51|P-value:3.74E-11||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MSW1(YDR268W)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NCR1(YPL006W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NEJ1(YLR265C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:NIF3(YGL221C)|FD-Score:4.57|P-value:2.40E-6||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PAF1(YBR279W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PBP1(YGR178C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PDH1(YPR002W)|FD-Score:4.97|P-value:3.29E-7||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PEP8(YJL053W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PIR3(YKL163W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:POA1(YBR022W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PSR1(YLL010C)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUF3(YLL013C)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:QCR6(YFR033C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RIM101(YHL027W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:ROM2(YLR371W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL22B(YFL034C-A)|FD-Score:-4.65|P-value:1.69E-6||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPL23B(YER117W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPS11A(YDR025W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RTT103(YDR289C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RTT109(YLL002W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SAC6(YDR129C)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAK1(YER129W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SAM1(YLR180W)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SAP30(YMR263W)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SIP5(YMR140W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SLT2(YHR030C)|FD-Score:4.35|P-value:6.72E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF3(YDL194W)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNO1(YMR095C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SPO11(YHL022C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPT7(YBR081C)|FD-Score:4.34|P-value:7.25E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:STO1(YMR125W)|FD-Score:5.28|P-value:6.45E-8||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUB1(YMR039C)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SYF2(YGR129W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:THI6(YPL214C)|FD-Score:4.9|P-value:4.91E-7||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:THP1(YOL072W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TPM1(YNL079C)|FD-Score:4.91|P-value:4.46E-7||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:UGA3(YDL170W)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS35(YJL154C)|FD-Score:4.53|P-value:2.92E-6||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS55(YJR044C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS61(YDR136C_d)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR232C(YBR232C_d)|FD-Score:4.27|P-value:9.99E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR061W(YCR061W_p)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDL094C(YDL094C_d)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR521W(YDR521W_d)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YEN1(YER041W)|FD-Score:6.09|P-value:5.48E-10||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFL019C(YFL019C_d)|FD-Score:6.19|P-value:3.07E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGK3(YOL128C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL010W(YGL010W_p)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR045C(YGR045C_d)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:-6.09|P-value:5.75E-10||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHI9(YHR029C)|FD-Score:-3.76|P-value:8.46E-5||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YIR024C(YIR024C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YKL222C(YKL222C)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR001C(YLR001C_p)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR184W(YLR184W_d)|FD-Score:7.05|P-value:8.80E-13||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR202C(YLR202C_d)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR278C(YLR278C_p)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YLR287C(YLR287C_p)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YLR455W(YLR455W_p)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR166C(YMR166C_p)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR294W-A(YMR294W-A_d)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL108C(YNL108C_p)|FD-Score:-4.51|P-value:3.31E-6||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOL036W(YOL036W_p)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL025C(YPL025C_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNR053C6.153.80E-100.59NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YPR165W5.561.36E-80.22RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YPL160W5.334.80E-80.51CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YDL058W4.827.04E-70.20USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YGR147C4.631.85E-60.56NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YBL014C4.072.34E-50.17RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YER003C3.904.81E-50.31PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YJL143W3.591.64E-40.32TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YPL063W3.285.25E-40.10TIM50Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
YNL075W3.187.41E-40.31IMP4Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
YGL247W2.870.002060.10BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YLR045C2.770.002800.03STU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
YNL126W2.740.003090.03SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YJL156C2.710.003410.00SSY5Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p
YDL163W_d2.700.003430.05YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR184W_d7.058.80E-13YLR184W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL154W6.513.74E-11MSH5Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
YFL019C_d6.193.07E-10YFL019C_dDubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene
YER041W6.095.48E-10YEN1Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
YLR172C5.773.97E-9DPH5Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
YMR125W5.286.45E-8STO1Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
YMR263W5.022.60E-7SAP30Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
YPR002W4.973.29E-7PDH1Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
YNL079C4.914.46E-7TPM1Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
YPL214C4.904.91E-7THI6Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YHR200W4.701.32E-6RPN10Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein
YOR198C4.612.00E-6BFR1Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
YDR245W4.612.06E-6MNN10Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
YOL036W_p4.602.15E-6YOL036W_pProtein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication
YKL222C4.602.15E-6YKL222CProtein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine

GO enrichment analysis for SGTC_2536
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1285.49E-23SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.116667cell wall signaling
0.1254.06E-22SGTC_20094012297 12.1 μMChembridge (Fragment library)455953070.0694444cell wall signaling
0.1241.29E-21SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.0813954cell wall signaling
0.1162.85E-19SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0810811cell wall signaling
0.1062.95E-16SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.101449cell wall signaling
0.1054.97E-16SGTC_30929117248 49.5 μMChembridge (Drug-like library)171418570.139241cell wall signaling
0.1041.23E-15SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.0833333cell wall signaling
0.1001.49E-14SGTC_23995884133 200.0 μMMiscellaneous7224450.0813954cell wall signaling
0.1001.61E-14SGTC_483fpl-64176 144.0 μMICCB bioactive library34230.0921053cell wall
0.0992.52E-14SGTC_422cantharidin 155.0 μMICCB bioactive library25450.140351cell wall signaling
0.0992.88E-14SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.15cell wall signaling
0.0976.86E-14SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0652174cell wall
0.0979.24E-14SGTC_3690957-0393 4.5 μMChemDiv (Drug-like library)69979920.0625cell wall signaling
0.0937.37E-13SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.109375cell wall signaling
0.0931.00E-12SGTC_31229126309 49.5 μMChembridge (Drug-like library)175912060.0352941cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_260nsc-652383.99 μM0.6538463246252Miscellaneous407.588183.84623endomembrane recycling
SGTC_249418-aminoabieta-8,11,13-triene sulfate3.66 μM0.65384616725076Microsource (Natural product library)383.545324.25835
SGTC_2517totarol10 μM0.383333460178Microsource (Natural product library)286.45166.00411
SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one66.67 μM0.3333336708561Microsource (Natural product library)228.32943.65701
SGTC_2616abietic acid100 μM0.3088246708678Microsource (Natural product library)302.4515.09312
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.2622956708760Microsource (Natural product library)216.31873.35611
SGTC_406estrone100 μM0.2318845870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.231884698TimTec (Pure natural product library)270.366083.93812DNA intercalators
SGTC_2644guaiazulene100 μM0.2264153515Microsource (Natural product library)198.303424.90500
SGTC_1767st04499270.3 μM0.2222229600693TimTec (Natural product derivative library)284.395963.50123