nsc-64875

3-(9-tert-butyl-3-azaspiro[5.5]undecan-3-yl)-N,N-dimethylpropan-1-amine

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_254
Screen concentration 3.8 μM
Source Miscellaneous
PubChem CID 56993
SMILES CC(C)(C)C1CCC2(CC1)CCN(CC2)CCCN(C)C
Standardized SMILES CN(C)CCCN1CCC2(CCC(CC2)C(C)(C)C)CC1
Molecular weight 294.5184
ALogP 4.06
H-bond donor count 0
H-bond acceptor count 2
Response signature ergosterol depletion effects on membrane

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.78
% growth inhibition (Hom. pool) 5.48


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 56993
Download HIP data (tab-delimited text)  (excel)
Gene:CDC20(YGL116W)|FD-Score:5.99|P-value:1.06E-9|Clearance:1.31||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC37(YDR168W)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.19||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:ESS1(YJR017C)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GFA1(YKL104C)|FD-Score:5.49|P-value:2.05E-8|Clearance:1.31||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:HEM12(YDR047W)|FD-Score:5.07|P-value:1.96E-7|Clearance:1.31||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HIP1(YGR191W)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.12||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:SPC34(YKR037C)|FD-Score:-3.91|P-value:4.70E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SUB2(YDL084W)|FD-Score:3.77|P-value:8.32E-5|Clearance:0.28||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SYF1(YDR416W)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TOP2(YNL088W)|FD-Score:-4.78|P-value:8.55E-7|Clearance:0||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL016C(YDL016C_d)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YNL247W(YNL247W)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:CDC20(YGL116W)|FD-Score:5.99|P-value:1.06E-9|Clearance:1.31||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC37(YDR168W)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.19||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:ESS1(YJR017C)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GFA1(YKL104C)|FD-Score:5.49|P-value:2.05E-8|Clearance:1.31||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:HEM12(YDR047W)|FD-Score:5.07|P-value:1.96E-7|Clearance:1.31||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HIP1(YGR191W)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.12||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:SPC34(YKR037C)|FD-Score:-3.91|P-value:4.70E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SUB2(YDL084W)|FD-Score:3.77|P-value:8.32E-5|Clearance:0.28||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SYF1(YDR416W)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TOP2(YNL088W)|FD-Score:-4.78|P-value:8.55E-7|Clearance:0||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL016C(YDL016C_d)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YNL247W(YNL247W)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 56993
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ADO1(YJR105W)|FD-Score:4.89|P-value:5.08E-7||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AGE2(YIL044C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIR2(YDL175C)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:APL2(YKL135C)|FD-Score:12.1|P-value:3.05E-34||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:APL4(YPR029C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:APM2(YHL019C)|FD-Score:8.07|P-value:3.66E-16||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:APS1(YLR170C)|FD-Score:5.22|P-value:8.78E-8||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ATG7(YHR171W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BCK1(YJL095W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BYE1(YKL005C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit Gene:CBF1(YJR060W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CKB1(YGL019W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:16.3|P-value:2.52E-60||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CTK3(YML112W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CWH41(YGL027C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:EAP1(YKL204W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ERG2(YMR202W)|FD-Score:16.3|P-value:5.87E-60||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GBP2(YCL011C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GCN5(YGR252W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:29.5|P-value:4.84E-192||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDS1(YOR355W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GTT3(YEL017W)|FD-Score:-4.45|P-value:4.21E-6||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:GVP36(YIL041W)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis Gene:GYP1(YOR070C)|FD-Score:13.1|P-value:1.11E-39||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOM6(YJR139C)|FD-Score:9.68|P-value:1.90E-22||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IMA5(YJL216C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IMP2'(YIL154C)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LAA1(YJL207C)|FD-Score:9.06|P-value:6.57E-20||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LAP2(YNL045W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LSM1(YJL124C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MKS1(YNL076W)|FD-Score:-3.75|P-value:8.70E-5||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:MOT3(YMR070W)|FD-Score:4.33|P-value:7.32E-6||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRPL40(YPL173W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTC4(YBR255W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MTG2(YHR168W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:NHX1(YDR456W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NTO1(YPR031W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:NUT1(YGL151W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OMA1(YKR087C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PKP2(YGL059W)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PPT2(YPL148C)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRO2(YOR323C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC7(YHR076W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAM1(YDL090C)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:6.37|P-value:9.27E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM4(YHL024W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RNH201(YNL072W)|FD-Score:3.9|P-value:4.71E-5||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL38(YLR325C)|FD-Score:4.97|P-value:3.29E-7||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPL41A(YDL184C)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:4.89|P-value:4.96E-7||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SKN1(YGR143W)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SLT2(YHR030C)|FD-Score:5.35|P-value:4.47E-8||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNC2(YOR327C)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SRF1(YDL133W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SWE1(YJL187C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:THP1(YOL072W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TVP18(YMR071C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VAN1(YML115C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:YDL118W(YDL118W_p)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:7.05|P-value:8.96E-13||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR271C(YDR271C_d)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR274C(YDR274C_d)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL034W(YFL034W_p)|FD-Score:20.9|P-value:3.07E-97||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YJL169W(YJL169W_d)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKR070W(YKR070W_p)|FD-Score:-3.84|P-value:6.13E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL007C(YLL007C_p)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR169W(YLR169W_d)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR171W(YLR171W_d)|FD-Score:8.92|P-value:2.37E-19||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML037C(YML037C_p)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNL035C(YNL035C_p)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR012W(YPR012W_d)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPT31(YER031C)|FD-Score:5.15|P-value:1.32E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ZIP1(YDR285W)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ZTA1(YBR046C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:ADK1(YDR226W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ADO1(YJR105W)|FD-Score:4.89|P-value:5.08E-7||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AGE2(YIL044C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIR2(YDL175C)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:APL2(YKL135C)|FD-Score:12.1|P-value:3.05E-34||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:APL4(YPR029C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:APM2(YHL019C)|FD-Score:8.07|P-value:3.66E-16||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:APS1(YLR170C)|FD-Score:5.22|P-value:8.78E-8||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ATG7(YHR171W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BCK1(YJL095W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BYE1(YKL005C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit Gene:CBF1(YJR060W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CKB1(YGL019W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:16.3|P-value:2.52E-60||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CTK3(YML112W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CWH41(YGL027C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:EAP1(YKL204W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ERG2(YMR202W)|FD-Score:16.3|P-value:5.87E-60||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GBP2(YCL011C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GCN5(YGR252W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:29.5|P-value:4.84E-192||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDS1(YOR355W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GTT3(YEL017W)|FD-Score:-4.45|P-value:4.21E-6||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:GVP36(YIL041W)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis Gene:GYP1(YOR070C)|FD-Score:13.1|P-value:1.11E-39||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOM6(YJR139C)|FD-Score:9.68|P-value:1.90E-22||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IMA5(YJL216C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IMP2'(YIL154C)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LAA1(YJL207C)|FD-Score:9.06|P-value:6.57E-20||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LAP2(YNL045W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LSM1(YJL124C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MKS1(YNL076W)|FD-Score:-3.75|P-value:8.70E-5||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:MOT3(YMR070W)|FD-Score:4.33|P-value:7.32E-6||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRPL40(YPL173W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTC4(YBR255W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MTG2(YHR168W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:NHX1(YDR456W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NTO1(YPR031W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:NUT1(YGL151W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OMA1(YKR087C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PKP2(YGL059W)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PPT2(YPL148C)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRO2(YOR323C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC7(YHR076W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAM1(YDL090C)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:6.37|P-value:9.27E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM4(YHL024W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RNH201(YNL072W)|FD-Score:3.9|P-value:4.71E-5||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL38(YLR325C)|FD-Score:4.97|P-value:3.29E-7||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPL41A(YDL184C)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:4.89|P-value:4.96E-7||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SKN1(YGR143W)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SLT2(YHR030C)|FD-Score:5.35|P-value:4.47E-8||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNC2(YOR327C)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SRF1(YDL133W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SWE1(YJL187C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:THP1(YOL072W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TVP18(YMR071C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VAN1(YML115C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:YDL118W(YDL118W_p)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:7.05|P-value:8.96E-13||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR271C(YDR271C_d)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR274C(YDR274C_d)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL034W(YFL034W_p)|FD-Score:20.9|P-value:3.07E-97||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YJL169W(YJL169W_d)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKR070W(YKR070W_p)|FD-Score:-3.84|P-value:6.13E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL007C(YLL007C_p)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR169W(YLR169W_d)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR171W(YLR171W_d)|FD-Score:8.92|P-value:2.37E-19||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML037C(YML037C_p)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNL035C(YNL035C_p)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR012W(YPR012W_d)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPT31(YER031C)|FD-Score:5.15|P-value:1.32E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ZIP1(YDR285W)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ZTA1(YBR046C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL116W5.991.06E-91.31CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YKL104C5.492.05E-81.31GFA1Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
YDR047W5.071.96E-71.31HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YDL084W3.778.32E-50.28SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDL016C_d3.492.42E-40.22YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YGR191W3.275.33E-40.12HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YDR168W3.158.21E-40.18CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YLR167W2.960.001520.04RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YML126C2.920.001750.01ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YOR151C2.910.001820.00RPB2RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
YMR146C2.900.001840.07TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YPL146C2.830.002310.05NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YKL182W2.780.002730.02FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YGR264C2.760.002910.06MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YER157W2.700.003510.08COG3Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL226C29.504.84E-192GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YFL034W_p20.903.07E-97YFL034W_pPutative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
YOR039W16.302.52E-60CKB2Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
YMR202W16.305.87E-60ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YOR070C13.101.11E-39GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YKL135C12.103.05E-34APL2Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
YJR139C9.681.90E-22HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YJL207C9.066.57E-20LAA1AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
YLR171W_d8.922.37E-19YLR171W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHL019C8.073.66E-16APM2Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
YDL119C_p7.058.96E-13YDL119C_pPutative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
YJL204C6.379.27E-11RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YHR030C5.354.47E-8SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YLR170C5.228.78E-8APS1Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
YER031C5.151.32E-7YPT31Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

GO enrichment analysis for SGTC_254
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4630SGTC_2014262-0159 12.8 μMChemDiv (Drug-like library)13410290.140351ergosterol depletion effects on membrane
0.4507.41E-292SGTC_262fenpropimorph 62.5 μMMiscellaneous916950.131148ergosterol depletion effects on membrane
0.4281.01E-261SGTC_2074185-0010 4.5 μMChemDiv (Drug-like library)37617530.0823529endomembrane recycling
0.4212.39E-252SGTC_6491123-0311 367.0 μMChemDiv (Drug-like library)6936290.030303endomembrane recycling
0.4152.94E-245SGTC_20064011487 200.0 μMChembridge (Fragment library)455952940.0178571endomembrane recycling
0.3967.00E-220SGTC_218alverine citrate 93.8 μMMiscellaneous217180.0980392fatty acid desaturase (OLE1)
0.3879.43E-210SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.115385plasma membrane duress
0.3853.58E-208SGTC_1784092-0806 87.5 μMChemDiv (Drug-like library)21690440.103448ergosterol depletion effects on membrane
0.3843.48E-207SGTC_1750st081859 2.4 μMTimTec (Natural product derivative library)52916950.103448endomembrane recycling
0.3827.28E-205SGTC_3204092-0846 5.3 μMChemDiv (Drug-like library)13593560.114286ergosterol depletion effects on membrane
0.3708.12E-191SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.0147059fatty acid desaturase (OLE1)
0.3662.33E-186SGTC_7680384-0033 77.3 μMChemDiv (Drug-like library)3965305NAfatty acid desaturase (OLE1)
0.3621.77E-182SGTC_258haloperidol 50.8 μMMiscellaneous35590.184615fatty acid desaturase (OLE1)
0.3624.31E-182SGTC_7881182-0090 188.0 μMChemDiv (Drug-like library)57151850.0166667endomembrane recycling
0.3611.39E-181SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.0882353fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2730imipramine71.33 μM0.2653063696Miscellaneous280.407264.38702
SGTC_2732terfenadine20 μM0.2380955405Miscellaneous471.673446.49823ERG2
SGTC_3014119-010024.5 μM0.2241382801ChemDiv (Drug-like library)314.852325.05202NEO1
SGTC_484loperamide105 μM0.2089553955Miscellaneous477.037524.63513
SGTC_2728chlorpromazine9.18 μM0.203392726Miscellaneous318.864164.73903
SGTC_14274048-4900550 nM0.18867924132586ChemDiv (Drug-like library)307.472365.79401
SGTC_2249haloperidol116.36 μM0.1846153559Miscellaneous375.8642233.88714ergosterol depletion effects on membrane
SGTC_258haloperidol50.78 μM0.1846153559Miscellaneous375.8642233.88714fatty acid desaturase (OLE1)
SGTC_2725haloperidol53.21 μM0.1846153559Miscellaneous375.8642233.88714plasma membrane duress
SGTC_5352293-412948.2 μM0.179104396118ChemDiv (Drug-like library)367.50773.77204NEO1
SGTC_9601222-01867.7 μM0.1718753126296ChemDiv (Drug-like library)357.572567.00402
SGTC_2720fluspirilene36.88 μM0.1714293396Miscellaneous475.5727465.54515ERG2