pimpinellin

5,6-dimethoxyfuro[2,3-h]chromen-2-one

From Heracleum laciniatum; cause of phototoxicity in patient with vitiligo.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2543
Screen concentration 60.7 μM
Source Microsource (Natural product library)
PubChem CID 4825
SMILES COC1=C(C2=C(C=CO2)C3=C1C=CC(=O)O3)OC
Standardized SMILES COc1c(OC)c2occc2c3OC(=O)C=Cc13
Molecular weight 246.2155
ALogP 2.17
H-bond donor count 0
H-bond acceptor count 5
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.99
% growth inhibition (Hom. pool) 6.71


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4825
Download HIP data (tab-delimited text)  (excel)
Gene:ARC35(YNR035C)|FD-Score:3.12|P-value:8.92E-4|Clearance:0.13||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:COG3(YER157W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.21||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSN1(YIR010W)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:ECM9(YKR004C)|FD-Score:-3.36|P-value:3.83E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:GWT1(YJL091C)|FD-Score:4.05|P-value:2.53E-5|Clearance:0.38||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:NUP49(YGL172W)|FD-Score:3.27|P-value:5.36E-4|Clearance:0.15||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PKC1(YBL105C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.38||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPL28(YGL103W)|FD-Score:-3.88|P-value:5.16E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RRP12(YPL012W)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:RSC6(YCR052W)|FD-Score:4.56|P-value:2.61E-6|Clearance:0.38||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:3.86|P-value:5.60E-5|Clearance:0.38||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC10(YLR166C)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.38||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:YCL041C(YCL041C_d)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:YTM1(YOR272W)|FD-Score:-3.12|P-value:9.19E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:ARC35(YNR035C)|FD-Score:3.12|P-value:8.92E-4|Clearance:0.13||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:COG3(YER157W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.21||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSN1(YIR010W)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:ECM9(YKR004C)|FD-Score:-3.36|P-value:3.83E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:GWT1(YJL091C)|FD-Score:4.05|P-value:2.53E-5|Clearance:0.38||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:NUP49(YGL172W)|FD-Score:3.27|P-value:5.36E-4|Clearance:0.15||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PKC1(YBL105C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.38||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPL28(YGL103W)|FD-Score:-3.88|P-value:5.16E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RRP12(YPL012W)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:RSC6(YCR052W)|FD-Score:4.56|P-value:2.61E-6|Clearance:0.38||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:3.86|P-value:5.60E-5|Clearance:0.38||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC10(YLR166C)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.38||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:YCL041C(YCL041C_d)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:YTM1(YOR272W)|FD-Score:-3.12|P-value:9.19E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4825
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:4.77|P-value:9.23E-7||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ARL3(YPL051W)|FD-Score:4.35|P-value:6.66E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP6(YLR085C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:AST2(YER101C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:ATP20(YPR020W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:AUA1(YFL010W-A)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BMH2(YDR099W)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BRO1(YPL084W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BTT1(YDR252W)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:BUD6(YLR319C)|FD-Score:-3.84|P-value:6.11E-5||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CEX1(YOR112W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COG5(YNL051W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.12|P-value:1.85E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRH1(YGR189C)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:DET1(YDR051C)|FD-Score:-3.91|P-value:4.63E-5||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:DUG1(YFR044C)|FD-Score:7.24|P-value:2.32E-13||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ENV9(YOR246C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERV46(YAL042W)|FD-Score:3.14|P-value:8.30E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FRA2(YGL220W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FUR4(YBR021W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL11(YOL051W)|FD-Score:3.2|P-value:6.75E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN5(YGR252W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GOS1(YHL031C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GYP5(YPL249C)|FD-Score:-6.47|P-value:4.77E-11||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HEM14(YER014W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HSP82(YPL240C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HUT1(YPL244C)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:IES5(YER092W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRS4(YKR019C)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KAR9(YPL269W)|FD-Score:-3.91|P-value:4.71E-5||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LDB19(YOR322C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LEA1(YPL213W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LOS1(YKL205W)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MCK1(YNL307C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MGR1(YCL044C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MNN4(YKL201C)|FD-Score:-4.34|P-value:7.25E-6||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MSF1(YPR047W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSK1(YNL073W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:MUB1(YMR100W)|FD-Score:-3.77|P-value:8.05E-5||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:PAN2(YGL094C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PCL1(YNL289W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PDA1(YER178W)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PGM3(YMR278W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PHM6(YDR281C)|FD-Score:-4.02|P-value:2.85E-5||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PHO89(YBR296C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PMP2(YEL017C-A)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PYC2(YBR218C)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RFM1(YOR279C)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIM1(YCR028C-A)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPL15B(YMR121C)|FD-Score:-3.84|P-value:6.20E-5||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPL22A(YLR061W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SCP160(YJL080C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCW10(YMR305C)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SKG3(YLR187W_p)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SOD1(YJR104C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPH1(YLR313C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPT10(YJL127C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRO7(YPR032W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STE13(YOR219C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:STP2(YHR006W)|FD-Score:-4.42|P-value:4.84E-6||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA8(YAL064C-A_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TES1(YJR019C)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:THO1(YER063W)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation Gene:TLG2(YOL018C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UME1(YPL139C)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VPS1(YKR001C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS75(YNL246W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:WTM1(YOR230W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBR285W(YBR285W_p)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCL023C(YCL023C_d)|FD-Score:-3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCR061W(YCR061W_p)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR018C(YDR018C_p)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YDR524W-C(YDR524W-C_p)|FD-Score:4.43|P-value:4.68E-6||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YDR541C(YDR541C_p)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YEL045C(YEL045C_d)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YGL034C(YGL034C_d)|FD-Score:4.28|P-value:9.32E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL149W(YGL149W_d)|FD-Score:-4.76|P-value:9.88E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YHR138C(YHR138C_p)|FD-Score:-3.75|P-value:8.95E-5||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function Gene:YIL141W(YIL141W_d)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL135W(YJL135W_d)|FD-Score:-4.27|P-value:9.77E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YLL054C(YLL054C_p)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR217W(YLR217W_d)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YLR264C-A(YLR264C-A_p)|FD-Score:4.98|P-value:3.12E-7||SGD DESC:Putative protein of unknown function Gene:YLR280C(YLR280C_d)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR334C(YLR334C_d)|FD-Score:-4.58|P-value:2.35E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Putative protein of unknown function Gene:YMD8(YML038C)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML003W(YML003W_p)|FD-Score:-4.33|P-value:7.62E-6||SGD DESC:Putative protein of unknown function Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YNL184C(YNL184C_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR012W(YOR012W_p)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Putative protein of unknown function Gene:YOR300W(YOR300W_d)|FD-Score:-4.07|P-value:2.36E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YPL068C(YPL068C_p)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPL264C(YPL264C_p)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPT31(YER031C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ADO1(YJR105W)|FD-Score:4.77|P-value:9.23E-7||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ARL3(YPL051W)|FD-Score:4.35|P-value:6.66E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP6(YLR085C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:AST2(YER101C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:ATP20(YPR020W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:AUA1(YFL010W-A)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BMH2(YDR099W)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BRO1(YPL084W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BTT1(YDR252W)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:BUD6(YLR319C)|FD-Score:-3.84|P-value:6.11E-5||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CEX1(YOR112W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COG5(YNL051W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.12|P-value:1.85E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRH1(YGR189C)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:DET1(YDR051C)|FD-Score:-3.91|P-value:4.63E-5||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:DUG1(YFR044C)|FD-Score:7.24|P-value:2.32E-13||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ENV9(YOR246C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERV46(YAL042W)|FD-Score:3.14|P-value:8.30E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FRA2(YGL220W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FUR4(YBR021W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL11(YOL051W)|FD-Score:3.2|P-value:6.75E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN5(YGR252W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GOS1(YHL031C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GYP5(YPL249C)|FD-Score:-6.47|P-value:4.77E-11||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HEM14(YER014W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HSP82(YPL240C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HUT1(YPL244C)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:IES5(YER092W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRS4(YKR019C)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KAR9(YPL269W)|FD-Score:-3.91|P-value:4.71E-5||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LDB19(YOR322C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LEA1(YPL213W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LOS1(YKL205W)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MCK1(YNL307C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MGR1(YCL044C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MNN4(YKL201C)|FD-Score:-4.34|P-value:7.25E-6||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MSF1(YPR047W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSK1(YNL073W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:MUB1(YMR100W)|FD-Score:-3.77|P-value:8.05E-5||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:PAN2(YGL094C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PCL1(YNL289W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PDA1(YER178W)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PGM3(YMR278W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PHM6(YDR281C)|FD-Score:-4.02|P-value:2.85E-5||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PHO89(YBR296C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PMP2(YEL017C-A)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PYC2(YBR218C)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RFM1(YOR279C)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIM1(YCR028C-A)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPL15B(YMR121C)|FD-Score:-3.84|P-value:6.20E-5||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPL22A(YLR061W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SCP160(YJL080C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCW10(YMR305C)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SKG3(YLR187W_p)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SOD1(YJR104C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPH1(YLR313C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPT10(YJL127C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRO7(YPR032W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STE13(YOR219C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:STP2(YHR006W)|FD-Score:-4.42|P-value:4.84E-6||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA8(YAL064C-A_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TES1(YJR019C)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:THO1(YER063W)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation Gene:TLG2(YOL018C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UME1(YPL139C)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VPS1(YKR001C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS75(YNL246W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:WTM1(YOR230W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBR285W(YBR285W_p)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCL023C(YCL023C_d)|FD-Score:-3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCR061W(YCR061W_p)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR018C(YDR018C_p)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YDR524W-C(YDR524W-C_p)|FD-Score:4.43|P-value:4.68E-6||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YDR541C(YDR541C_p)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YEL045C(YEL045C_d)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YGL034C(YGL034C_d)|FD-Score:4.28|P-value:9.32E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL149W(YGL149W_d)|FD-Score:-4.76|P-value:9.88E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YHR138C(YHR138C_p)|FD-Score:-3.75|P-value:8.95E-5||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function Gene:YIL141W(YIL141W_d)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL135W(YJL135W_d)|FD-Score:-4.27|P-value:9.77E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YLL054C(YLL054C_p)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR217W(YLR217W_d)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YLR264C-A(YLR264C-A_p)|FD-Score:4.98|P-value:3.12E-7||SGD DESC:Putative protein of unknown function Gene:YLR280C(YLR280C_d)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR334C(YLR334C_d)|FD-Score:-4.58|P-value:2.35E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Putative protein of unknown function Gene:YMD8(YML038C)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML003W(YML003W_p)|FD-Score:-4.33|P-value:7.62E-6||SGD DESC:Putative protein of unknown function Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YNL184C(YNL184C_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR012W(YOR012W_p)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Putative protein of unknown function Gene:YOR300W(YOR300W_d)|FD-Score:-4.07|P-value:2.36E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YPL068C(YPL068C_p)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPL264C(YPL264C_p)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPT31(YER031C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCR052W4.562.61E-60.39RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YBL105C4.261.03E-50.39PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YLR166C4.131.79E-50.39SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YJL091C4.052.53E-50.39GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YML127W3.865.60E-50.39RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YER157W3.482.52E-40.21COG3Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGL172W3.275.36E-40.15NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YNR035C3.128.92E-40.13ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YBR002C2.990.001390.12RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YPL028W2.870.002020.05ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YJL011C2.830.002340.15RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YPR165W2.680.003700.03RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YGR277C2.650.003990.03CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YLR033W2.620.004370.06RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YJL039C2.560.005210.02NUP192Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFR044C7.242.32E-13DUG1Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
YLR264C-A_p4.983.12E-7YLR264C-A_pPutative protein of unknown function
YJR105W4.779.23E-7ADO1Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
YLR061W4.741.08E-6RPL22ARibosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
YPL244C4.711.25E-6HUT1Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
YKR019C4.434.66E-6IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YDR524W-C_p4.434.68E-6YDR524W-C_pPutative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin
YPL051W4.356.66E-6ARL3GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
YGL034C_d4.289.32E-6YGL034C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YML071C4.121.85E-5COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YJL080C4.111.99E-5SCP160Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
YER101C4.102.05E-5AST2Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
YOR230W4.062.47E-5WTM1Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
YPL139C3.855.81E-5UME1Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p
YBR218C3.817.01E-5PYC2Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2543
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1694.46E-39SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.047619Golgi
0.1504.18E-31SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0350877Golgi
0.1483.17E-30SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.0666667Golgi
0.1425.13E-28SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0379747Golgi
0.1361.09E-25SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.122807Golgi
0.1361.23E-25SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0655738Golgi
0.1338.08E-25SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.0384615Golgi
0.1271.38E-22SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.0461538Golgi
0.1241.32E-21SGTC_1078idebenone 36.1 μMMiscellaneous36860.125Golgi
0.1223.48E-21SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.2RSC complex & mRNA processing
0.1224.74E-21SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.0724638Golgi
0.1226.32E-21SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.0923077
0.1181.01E-19SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.140351Golgi
0.1163.15E-19SGTC_389tpck 16.7 μMMiscellaneous4396470.0735294
0.1141.44E-18SGTC_2811672-3440 14.9 μMChemDiv (Drug-like library)68330000.0533333Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2544sphondin68.51 μM0.568182108104Microsource (Natural product library)216.189522.18704RNA pol III & RNase P/MRP
SGTC_15258-methoxypsoralen92.5 μM0.5333334114TimTec (Pure natural product library)216.189522.18704DNA damage response
SGTC_2556citropten84.59 μM0.3404262775Microsource (Natural product library)206.19471.86604
SGTC_2612dictamnine15.46 μM0.30612268085Microsource (Natural product library)199.205362.50203RNA pol III & RNase P/MRP
SGTC_2423st07711987.2 μM0.288462107936TimTec (Natural product derivative library)229.231342.48604
SGTC_1120ficusin1.79 μM0.2857146199TimTec (Natural product library)186.163542.20303
SGTC_1114scopoletin1.73 μM0.285280460TimTec (Natural product library)192.168121.64114
SGTC_1168k815-000480.5 μM0.269231642433ChemDiv (Drug-like library)201.178181.83604DNA damage response
SGTC_7234182-0001115 μM0.259259744821ChemDiv (Drug-like library)218.248463.27203
SGTC_2600tangeritin87.95 μM0.25925968077Microsource (Natural product library)372.36863.05407