4-methylesculetin

6,7-dihydroxy-4-methylchromen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2553
Screen concentration 85.1 μM
Source Microsource (Natural product library)
PubChem CID 5319502
SMILES CC1=CC(=O)OC2=CC(=C(C=C12)O)O
Standardized SMILES CC1=CC(=O)Oc2cc(O)c(O)cc12
Molecular weight 192.1681
ALogP 1.86
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.53
% growth inhibition (Hom. pool) 7.68


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5319502
Download HIP data (tab-delimited text)  (excel)
Gene:ALR1(YOL130W)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.02||SGD DESC:Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions Gene:ARC35(YNR035C)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.12||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ENP1(YBR247C)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.21||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:ERG12(YMR208W)|FD-Score:-6.29|P-value:1.61E-10|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG8(YMR220W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.12||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MTR10(YOR160W)|FD-Score:-3.19|P-value:7.18E-4|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NIP7(YPL211W)|FD-Score:5.37|P-value:3.84E-8|Clearance:1.14||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NOC4(YPR144C)|FD-Score:4.03|P-value:2.83E-5|Clearance:0.15||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:OST2(YOR103C)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.03||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RPO26(YPR187W)|FD-Score:-3.67|P-value:1.21E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPS5(YJR123W)|FD-Score:3.87|P-value:5.40E-5|Clearance:0.2||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC26(YDR238C)|FD-Score:3.24|P-value:5.96E-4|Clearance:0.16||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SKI6(YGR195W)|FD-Score:3.56|P-value:1.87E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SUI2(YJR007W)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.11||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:YGR190C(YGR190C_d)|FD-Score:-4.36|P-value:6.54E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YJL086C(YJL086C_d)|FD-Score:-3.1|P-value:9.81E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:ALR1(YOL130W)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.02||SGD DESC:Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions Gene:ARC35(YNR035C)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.12||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ENP1(YBR247C)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.21||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:ERG12(YMR208W)|FD-Score:-6.29|P-value:1.61E-10|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG8(YMR220W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.12||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MTR10(YOR160W)|FD-Score:-3.19|P-value:7.18E-4|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NIP7(YPL211W)|FD-Score:5.37|P-value:3.84E-8|Clearance:1.14||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NOC4(YPR144C)|FD-Score:4.03|P-value:2.83E-5|Clearance:0.15||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:OST2(YOR103C)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.03||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RPO26(YPR187W)|FD-Score:-3.67|P-value:1.21E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPS5(YJR123W)|FD-Score:3.87|P-value:5.40E-5|Clearance:0.2||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC26(YDR238C)|FD-Score:3.24|P-value:5.96E-4|Clearance:0.16||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SKI6(YGR195W)|FD-Score:3.56|P-value:1.87E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SUI2(YJR007W)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.11||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:YGR190C(YGR190C_d)|FD-Score:-4.36|P-value:6.54E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YJL086C(YJL086C_d)|FD-Score:-3.1|P-value:9.81E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5319502
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AIM29(YKR074W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:AMA1(YGR225W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:ATG17(YLR423C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:CBP1(YJL209W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CDC50(YCR094W)|FD-Score:4.84|P-value:6.62E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CMR1(YDL156W)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:DBF2(YGR092W)|FD-Score:8.66|P-value:2.41E-18||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DIA4(YHR011W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DUN1(YDL101C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:GAL11(YOL051W)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GSP2(YOR185C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:-4.75|P-value:1.01E-6||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBT1(YDL223C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HST2(YPL015C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:LGE1(YPL055C)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LHS1(YKL073W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LOT5(YKL183W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MAG1(YER142C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MAL11(YGR289C)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MBP1(YDL056W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MDM31(YHR194W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MDM34(YGL219C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MDM35(YKL053C-A)|FD-Score:4.38|P-value:5.97E-6||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MFM1(YPL060W)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MGM1(YOR211C)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MRPL11(YDL202W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL16(YBL038W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL32(YCR003W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MST1(YKL194C)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NPR2(YEL062W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:OCA4(YCR095C_p)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OCH1(YGL038C)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:PEP7(YDR323C)|FD-Score:-4.09|P-value:2.12E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET100(YDR079W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PHA2(YNL316C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PST2(YDR032C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PUF2(YPR042C)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RDL1(YOR285W)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RHO4(YKR055W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPN13(YLR421C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPN4(YDL020C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14B(YJL191W)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:SBE22(YHR103W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SLS1(YLR139C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SPO75(YLL005C)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SSK1(YLR006C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:STB4(YMR019W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STE50(YCL032W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUR4(YLR372W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT1(YBR069C)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA5(YLR426W_p)|FD-Score:7.41|P-value:6.10E-14||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TLG2(YOL018C)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA23(YMR269W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRP3(YKL211C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VNX1(YNL321W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS73(YGL104C)|FD-Score:-3.92|P-value:4.40E-5||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBR134W(YBR134W_d)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR007C(YCR007C_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR433W(YDR433W_d)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Putative protein of unknown function Gene:YHL017W(YHL017W_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YHR080C(YHR080C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIL092W(YIL092W_p)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL120W(YJL120W_d)|FD-Score:4.46|P-value:4.12E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL023W(YKL023W_p)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR072W(YLR072W_p)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR173W(YLR173W_p)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Putative protein of unknown function Gene:YLR252W(YLR252W_d)|FD-Score:4.32|P-value:7.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR412C-A(YLR412C-A_p)|FD-Score:5.81|P-value:3.05E-9||SGD DESC:Putative protein of unknown function Gene:YML003W(YML003W_p)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Putative protein of unknown function Gene:YNL144C(YNL144C_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YNL203C(YNL203C_d)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR066C(YNR066C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR199W(YOR199W_d)|FD-Score:6.63|P-value:1.66E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL039W(YPL039W_p)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL062W(YPL062W_d)|FD-Score:-6.82|P-value:4.57E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR089W(YPR089W_p)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:ADE4(YMR300C)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AIM29(YKR074W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:AMA1(YGR225W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:ATG17(YLR423C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:CBP1(YJL209W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CDC50(YCR094W)|FD-Score:4.84|P-value:6.62E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CMR1(YDL156W)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:DBF2(YGR092W)|FD-Score:8.66|P-value:2.41E-18||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DIA4(YHR011W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DUN1(YDL101C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:GAL11(YOL051W)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GSP2(YOR185C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:-4.75|P-value:1.01E-6||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBT1(YDL223C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HST2(YPL015C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:LGE1(YPL055C)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LHS1(YKL073W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LOT5(YKL183W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MAG1(YER142C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MAL11(YGR289C)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MBP1(YDL056W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MDM31(YHR194W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MDM34(YGL219C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MDM35(YKL053C-A)|FD-Score:4.38|P-value:5.97E-6||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MFM1(YPL060W)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MGM1(YOR211C)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MRPL11(YDL202W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL16(YBL038W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL32(YCR003W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MST1(YKL194C)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NPR2(YEL062W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:OCA4(YCR095C_p)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OCH1(YGL038C)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:PEP7(YDR323C)|FD-Score:-4.09|P-value:2.12E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET100(YDR079W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PHA2(YNL316C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PST2(YDR032C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PUF2(YPR042C)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RDL1(YOR285W)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RHO4(YKR055W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPN13(YLR421C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPN4(YDL020C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14B(YJL191W)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:SBE22(YHR103W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SLS1(YLR139C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SPO75(YLL005C)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SSK1(YLR006C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:STB4(YMR019W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STE50(YCL032W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUR4(YLR372W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT1(YBR069C)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA5(YLR426W_p)|FD-Score:7.41|P-value:6.10E-14||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TLG2(YOL018C)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA23(YMR269W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRP3(YKL211C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VNX1(YNL321W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS73(YGL104C)|FD-Score:-3.92|P-value:4.40E-5||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBR134W(YBR134W_d)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR007C(YCR007C_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR433W(YDR433W_d)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Putative protein of unknown function Gene:YHL017W(YHL017W_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YHR080C(YHR080C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIL092W(YIL092W_p)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL120W(YJL120W_d)|FD-Score:4.46|P-value:4.12E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL023W(YKL023W_p)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR072W(YLR072W_p)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR173W(YLR173W_p)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Putative protein of unknown function Gene:YLR252W(YLR252W_d)|FD-Score:4.32|P-value:7.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR412C-A(YLR412C-A_p)|FD-Score:5.81|P-value:3.05E-9||SGD DESC:Putative protein of unknown function Gene:YML003W(YML003W_p)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Putative protein of unknown function Gene:YNL144C(YNL144C_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YNL203C(YNL203C_d)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR066C(YNR066C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR199W(YOR199W_d)|FD-Score:6.63|P-value:1.66E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL039W(YPL039W_p)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL062W(YPL062W_d)|FD-Score:-6.82|P-value:4.57E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR089W(YPR089W_p)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL211W5.373.84E-81.14NIP7Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
YBR247C4.231.16E-50.21ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
YPR144C4.032.83E-50.15NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YJR123W3.875.40E-50.20RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YJR007W3.671.23E-40.11SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YGR195W3.561.87E-40.04SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YOL130W3.522.17E-40.02ALR1Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
YMR220W3.502.31E-40.12ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YOR103C3.383.57E-40.03OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YNR035C3.363.95E-40.12ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YDR238C3.245.96E-40.16SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YGL150C3.080.001040.12INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YFL045C2.960.001540.01SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YOR361C2.950.001580.04PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YBR202W2.920.001770.01MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR092W8.662.41E-18DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YLR426W_p7.416.10E-14TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YOR199W_d6.631.66E-11YOR199W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR412C-A_p5.813.05E-9YLR412C-A_pPutative protein of unknown function
YPR042C5.091.77E-7PUF2Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
YMR300C4.993.09E-7ADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
YCR094W4.846.62E-7CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YNL321W4.691.38E-6VNX1Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate
YPL015C4.464.01E-6HST2Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
YJL120W_d4.464.12E-6YJL120W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
YGL038C4.464.13E-6OCH1Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
YHR194W4.415.17E-6MDM31Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
YKL053C-A4.385.97E-6MDM35Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
YLR252W_d4.327.92E-6YLR252W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism
YLR139C4.181.44E-5SLS1Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery

GO enrichment analysis for SGTC_2553
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1223.68E-21SGTC_1651st011928 86.1 μMTimTec (Natural product derivative library)7201980.0892857RSC complex & mRNA processing
0.0931.09E-12SGTC_20585236698 133.0 μMChembridge (Fragment library)7666650.0555556
0.0791.52E-9SGTC_29107948405 26.0 μMChembridge (Drug-like library)29713280.0704225plasma membrane duress
0.0781.92E-9SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.175439
0.0782.09E-9SGTC_1470k295-0292 231.0 μMChemDiv (Drug-like library)50456120.0735294
0.0741.60E-8SGTC_5953909-8003 73.7 μMChemDiv (Drug-like library)6995170.0350877
0.0731.74E-8SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.0847458
0.0672.84E-7SGTC_10144130-3005 69.3 μMChemDiv (Drug-like library)7436080.0701754
0.0673.38E-7SGTC_460329-0187a 2.2 μMChemDiv (Drug-like library)464948170.0925926
0.0673.19E-7SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.0681818TRP & mitochondrial translation
0.0663.77E-7SGTC_267212a-hydroxy-5-deoxydehydromunduserone 100.0 μMMicrosource (Natural product library)46208980.15
0.0664.07E-7SGTC_1623st003709 20.3 μMTimTec (Natural product derivative library)161926180.0714286calcium & mitochondrial duress
0.0655.24E-7SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.191489superoxide
0.0649.57E-7SGTC_13531498-1206 8.1 μMChemDiv (Drug-like library)67568080.115385mitochondrial response to ROS
0.0621.63E-6SGTC_28659030313 52.0 μMChembridge (Drug-like library)425627540.0967742

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1518st03856790.8 μM0.58823577966TimTec (Pure natural product library)220.221282.31304
SGTC_15447-methoxy-4-methylcoumarin105 μM0.513514390807TimTec (Pure natural product library)190.19532.32903
SGTC_1900st06020072.8 μM0.475688945TimTec (Natural product derivative library)204.221882.67803
SGTC_1904st06022578 μM0.47566595TimTec (Natural product derivative library)204.221882.67803
SGTC_1926methyl fluorone black53.6 μM0.47572721TimTec (Natural product derivative library)258.22621.61735DNA intercalators
SGTC_1526st05151875 μM0.444444673993TimTec (Pure natural product library)266.677043.44504
SGTC_1905st06020784.5 μM0.4318181948547TimTec (Natural product derivative library)216.232582.94603
SGTC_7844579-0127173 μM0.4318181948547ChemDiv (Drug-like library)216.232582.94603
SGTC_1548st06146890.8 μM0.390244390799TimTec (Pure natural product library)220.221282.31304
SGTC_1841st05414870 μM0.38675442TimTec (Natural product derivative library)285.29462.79505