cholic acid

methyl 4-[(3R,5S,7R,10S,12S,13R,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoate

Influences liver metabolism.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2555
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 6708657
SMILES CC(CCC(=O)OC)C1CCC2C1(C(CC3C2C(CC4C3(CCC(C4)O)C)O)O)C
Standardized SMILES COC(=O)CCC(C)C1CCC2C3C(O)CC4CC(O)CCC4(C)C3CC(O)C12C
Molecular weight 422.598
ALogP 3.14
H-bond donor count 3
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.6
% growth inhibition (Hom. pool) 6.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6708657
Download HIP data (tab-delimited text)  (excel)
Gene:DED81(YHR019C)|FD-Score:-4.5|P-value:3.39E-6|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:DSN1(YIR010W)|FD-Score:-4.37|P-value:6.19E-6|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:FAD1(YDL045C)|FD-Score:-3.19|P-value:7.17E-4|Clearance:0||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:MYO1(YHR023W)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.37||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NIP7(YPL211W)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.37||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:SEN1(YLR430W)|FD-Score:-3.73|P-value:9.54E-5|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:VTI1(YMR197C)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.37||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YJL086C(YJL086C_d)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.37||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YOR282W(YOR282W_d)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.37||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:YPI1(YFR003C)|FD-Score:-3.22|P-value:6.48E-4|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:DED81(YHR019C)|FD-Score:-4.5|P-value:3.39E-6|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:DSN1(YIR010W)|FD-Score:-4.37|P-value:6.19E-6|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:FAD1(YDL045C)|FD-Score:-3.19|P-value:7.17E-4|Clearance:0||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:MYO1(YHR023W)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.37||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NIP7(YPL211W)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.37||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:SEN1(YLR430W)|FD-Score:-3.73|P-value:9.54E-5|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:VTI1(YMR197C)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.37||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YJL086C(YJL086C_d)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.37||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YOR282W(YOR282W_d)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.37||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:YPI1(YFR003C)|FD-Score:-3.22|P-value:6.48E-4|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6708657
Download HOP data (tab-delimited text)  (excel)
Gene:ACF2(YLR144C)|FD-Score:-5.31|P-value:5.60E-8||SGD DESC:Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress Gene:AGC1(YPR021C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ARO2(YGL148W)|FD-Score:-3.88|P-value:5.17E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARP1(YHR129C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ATP20(YPR020W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:CAD1(YDR423C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:DBF2(YGR092W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DHH1(YDL160C)|FD-Score:5.21|P-value:9.31E-8||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:EBS1(YDR206W)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:EGD2(YHR193C)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ERG4(YGL012W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GAL2(YLR081W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GLC8(YMR311C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPM2(YDL021W)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GTO3(YMR251W)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HPM1(YIL110W)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IRC21(YMR073C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IST3(YIR005W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:LEM3(YNL323W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LOS1(YKL205W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MRP21(YBL090W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL51(YPR100W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NGG1(YDR176W)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NUP120(YKL057C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OAF3(YKR064W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:OCA1(YNL099C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:ROM2(YLR371W)|FD-Score:7.68|P-value:8.02E-15||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL12A(YEL054C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:6.57|P-value:2.51E-11||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPP1A(YDL081C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPP1B(YDL130W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RSM25(YIL093C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM28(YDR494W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SFG1(YOR315W)|FD-Score:-3.97|P-value:3.59E-5||SGD DESC:Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate Gene:SHE4(YOR035C)|FD-Score:4.85|P-value:6.13E-7||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIP18(YMR175W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SLA1(YBL007C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SMF1(YOL122C)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins Gene:SNX4(YJL036W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SNZ2(YNL333W)|FD-Score:-3.82|P-value:6.74E-5||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:TAT1(YBR069C)|FD-Score:-4.17|P-value:1.50E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TGS1(YPL157W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOM1(YDR457W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRP1(YDR007W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UPC2(YDR213W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:7.3|P-value:1.42E-13||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS28(YPL065W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS41(YDR080W)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS73(YGL104C)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBR284W(YBR284W_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDL119C(YDL119C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR379C-A(YDR379C-A)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YDR524W-C(YDR524W-C_p)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YGL072C(YGL072C_d)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL159W(YGL159W_p)|FD-Score:-3.74|P-value:9.05E-5||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHR138C(YHR138C_p)|FD-Score:-3.83|P-value:6.43E-5||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YIL014C-A(YIL014C-A_p)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Putative protein of unknown function Gene:YIR044C(YIR044C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL007C(YJL007C_d)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR079W(YJR079W_p)|FD-Score:-4|P-value:3.13E-5||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YKE4(YIL023C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL077W(YKL077W_p)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR279W(YLR279W_d)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR310C(YMR310C_p)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YOL050C(YOL050C_d)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR019W(YOR019W)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR055W(YOR055W_d)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL062W(YPL062W_d)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:ACF2(YLR144C)|FD-Score:-5.31|P-value:5.60E-8||SGD DESC:Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress Gene:AGC1(YPR021C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ARO2(YGL148W)|FD-Score:-3.88|P-value:5.17E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARP1(YHR129C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ATP20(YPR020W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:CAD1(YDR423C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:DBF2(YGR092W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DHH1(YDL160C)|FD-Score:5.21|P-value:9.31E-8||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:EBS1(YDR206W)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:EGD2(YHR193C)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ERG4(YGL012W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GAL2(YLR081W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GLC8(YMR311C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPM2(YDL021W)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GTO3(YMR251W)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HPM1(YIL110W)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IRC21(YMR073C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IST3(YIR005W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:LEM3(YNL323W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LOS1(YKL205W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MRP21(YBL090W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL51(YPR100W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NGG1(YDR176W)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NUP120(YKL057C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OAF3(YKR064W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:OCA1(YNL099C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:ROM2(YLR371W)|FD-Score:7.68|P-value:8.02E-15||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL12A(YEL054C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:6.57|P-value:2.51E-11||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPP1A(YDL081C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPP1B(YDL130W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RSM25(YIL093C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM28(YDR494W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SFG1(YOR315W)|FD-Score:-3.97|P-value:3.59E-5||SGD DESC:Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate Gene:SHE4(YOR035C)|FD-Score:4.85|P-value:6.13E-7||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIP18(YMR175W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SLA1(YBL007C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SMF1(YOL122C)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins Gene:SNX4(YJL036W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SNZ2(YNL333W)|FD-Score:-3.82|P-value:6.74E-5||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:TAT1(YBR069C)|FD-Score:-4.17|P-value:1.50E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TGS1(YPL157W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOM1(YDR457W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRP1(YDR007W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UPC2(YDR213W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:7.3|P-value:1.42E-13||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS28(YPL065W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS41(YDR080W)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS73(YGL104C)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBR284W(YBR284W_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDL119C(YDL119C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR379C-A(YDR379C-A)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YDR524W-C(YDR524W-C_p)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YGL072C(YGL072C_d)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL159W(YGL159W_p)|FD-Score:-3.74|P-value:9.05E-5||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHR138C(YHR138C_p)|FD-Score:-3.83|P-value:6.43E-5||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YIL014C-A(YIL014C-A_p)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Putative protein of unknown function Gene:YIR044C(YIR044C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL007C(YJL007C_d)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR079W(YJR079W_p)|FD-Score:-4|P-value:3.13E-5||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YKE4(YIL023C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL077W(YKL077W_p)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR279W(YLR279W_d)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR310C(YMR310C_p)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YOL050C(YOL050C_d)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR019W(YOR019W)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR055W(YOR055W_d)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL062W(YPL062W_d)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR197C3.502.35E-40.37VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YHR023W3.472.62E-40.37MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
YOR282W_d3.442.86E-40.37YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YPL211W3.294.98E-40.37NIP7Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
YJL086C_d3.265.61E-40.37YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YGR128C2.890.001950.08UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YOR206W2.810.002480.11NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YAL043C2.700.003480.06PTA1Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
YDR064W2.640.004140.01RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YGL116W2.630.004240.01CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YHR085W2.620.004370.06IPI1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
YOR329C2.560.005260.02SCD5Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
YBR192W2.540.005580.01RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YDL017W2.530.005750.01CDC7DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p
YBR254C2.510.005970.04TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR371W7.688.02E-15ROM2GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p
YIL008W7.301.42E-13URM1Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
YMR194W6.572.51E-11RPL36ARibosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
YIL014C-A_p5.783.70E-9YIL014C-A_pPutative protein of unknown function
YDL160C5.219.31E-8DHH1Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
YDR524W-C_p5.072.02E-7YDR524W-C_pPutative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin
YOR035C4.856.13E-7SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YPL062W_d4.711.23E-6YPL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
YDL081C4.701.28E-6RPP1ARibosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
YPL157W4.661.59E-6TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YIL110W4.641.77E-6HPM1AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
YDR457W4.337.49E-6TOM1E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
YKL126W4.261.02E-5YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YMR310C_p4.251.07E-5YMR310C_pPutative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene
YMR311C4.221.22E-5GLC8Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2555
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0953.01E-13SGTC_1545lovastatin 49.4 μMTimTec (Pure natural product library)129236120.14
0.0921.30E-12SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.0631579ERG2
0.0863.61E-11SGTC_2670beta-carotene 100.0 μMMicrosource (Natural product library)52804890.0740741
0.0823.44E-10SGTC_2405ticlatone 3.5 μMMiscellaneous62580.0128205
0.0815.92E-10SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.07ERG2
0.0791.34E-9SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.050505
0.0773.36E-9SGTC_14954469-0029 237.0 μMChemDiv (Drug-like library)7936800.0108696
0.0759.38E-9SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.0222222ERG2
0.0759.75E-9SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.028169
0.0741.52E-8SGTC_487nimodipine 119.0 μMMiscellaneous44970.103093
0.0741.57E-8SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0537634ERG2
0.0731.71E-8SGTC_1856st057054 55.7 μMTimTec (Natural product derivative library)12660180.0306122
0.0714.19E-8SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.0408163translation
0.0714.24E-8SGTC_1106tegaserod maleate 11.7 μMNIH Clinical Collection69183690.050505
0.0715.61E-8SGTC_20275150920 200.0 μMChembridge (Fragment library)7867150.0454545

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1517deoxycholic acid methyl ester49.2 μM0.754098229346TimTec (Pure natural product library)406.598584.30724
SGTC_2531methyl deoxycholate70.07 μM0.75409816667744Microsource (Natural product library)406.598584.30724
SGTC_2687chenodiol100 μM0.6290325702181Microsource (Natural product library)392.5724.01434
SGTC_1560lithocholic acid53.1 μM0.4925379903TimTec (Pure natural product library)376.57265.18423
SGTC_2621lithochol-11-enic acid100 μM0.4266676708711Microsource (Natural product library)374.556724.73923
SGTC_2628lathosterol100 μM0.3289476710616Microsource (Natural product library)386.653547.37611
SGTC_1572epiandrosterone68.9 μM0.277778441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.277778441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.2777782754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_2642cholecalciferol100 μM0.2159099821465Microsource (Natural product library)384.637667.75511
SGTC_1587estradiol valerate56.1 μM0.21111113791TimTec (Pure natural product library)356.498385.79613