tomatine

(2R,3R,4S,5S,6R)-2-[(2R,3R,4S,5R)-2-[(2R,3R,4S,5R,6R)-2-[(2R,3R,4R,5R,6S)-6-[(1S,2S,5'S,7S,9aS,11aR)-1,5',9a,11a-tetramethylspiro[3a,3b,4,5,5a,6,7,8,9,9b,10,11-dodecahydro-1H-naphtho[1,2-g][1]benzofuran-2,2'-piperidine]-7-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol

Tomatine is a toxic glycoalkaloid found in the stems and leaves of tomato plants with fungicidal properties.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2563
Screen concentration 8.5 μM
Source Microsource (Natural product library)
PubChem CID 6708777
SMILES CC1CCC2(C(C3(CCC4C(C3O2)CCC5C4(CCC(C5)OC6C(C(C(C(O6)CO)OC7C(C(C(C(O7)CO)O)OC8C(C(C(CO8)O)O)OC9C(C(C(C(O9)CO)O)O)O)O)O)O)C)C)C)NC1
Standardized SMILES CC1CCC2(NC1)OC3C4CCC5CC(CCC5(C)C4CCC3(C)C2C)OC6OC(CO)C(OC7OC(CO)C(O)C(OC8OCC(O)C(O)C8OC9OC(CO)C(O)C(O)C9O)C7O)C(O)C6O
Molecular weight 994.1243
ALogP -2.31
H-bond donor count 13
H-bond acceptor count 22
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.48
% growth inhibition (Hom. pool) 3.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6708777
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:-4.92|P-value:4.40E-7|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:ARB1(YER036C)|FD-Score:5.23|P-value:8.30E-8|Clearance:0.37||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:BFR2(YDR299W)|FD-Score:7.41|P-value:6.36E-14|Clearance:0.87||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:BUR6(YER159C)|FD-Score:6.02|P-value:8.81E-10|Clearance:0.25||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CDC1(YDR182W)|FD-Score:3.74|P-value:9.09E-5|Clearance:0.13||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:ERG11(YHR007C)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.23||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GRS1(YBR121C)|FD-Score:-4.39|P-value:5.72E-6|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:5.77|P-value:4.08E-9|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAK21(YDR060W)|FD-Score:-4.98|P-value:3.18E-7|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:NSE5(YML023C)|FD-Score:-3.43|P-value:2.97E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:POP8(YBL018C)|FD-Score:3.31|P-value:4.73E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP3(YDR473C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.03||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RAM2(YKL019W)|FD-Score:5.66|P-value:7.43E-9|Clearance:0.43||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RIB3(YDR487C)|FD-Score:4.08|P-value:2.29E-5|Clearance:0.29||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RIX1(YHR197W)|FD-Score:-3.29|P-value:5.04E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPP1(YHR062C)|FD-Score:-5.99|P-value:1.07E-9|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPR2(YIR015W)|FD-Score:3.1|P-value:9.59E-4|Clearance:0||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPT4(YOR259C)|FD-Score:4.73|P-value:1.12E-6|Clearance:0.08||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RSC8(YFR037C)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SAH1(YER043C)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.12||SGD DESC:S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor Gene:SEC62(YPL094C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.05||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SKP1(YDR328C)|FD-Score:8.27|P-value:6.44E-17|Clearance:0.87||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SPC42(YKL042W)|FD-Score:4.86|P-value:5.79E-7|Clearance:0.13||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPT15(YER148W)|FD-Score:-3.2|P-value:6.81E-4|Clearance:0||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TAF1(YGR274C)|FD-Score:-3.19|P-value:7.07E-4|Clearance:0||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TFA1(YKL028W)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.17||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFB1(YDR311W)|FD-Score:4.65|P-value:1.66E-6|Clearance:0.1||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:YDR187C(YDR187C_d)|FD-Score:3.79|P-value:7.68E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR115C(YGR115C_d)|FD-Score:4.55|P-value:2.74E-6|Clearance:0.3||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJR023C(YJR023C_d)|FD-Score:6.53|P-value:3.18E-11|Clearance:0.52||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:YLL037W(YLL037W_d)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Gene:APC5(YOR249C)|FD-Score:-4.92|P-value:4.40E-7|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:ARB1(YER036C)|FD-Score:5.23|P-value:8.30E-8|Clearance:0.37||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:BFR2(YDR299W)|FD-Score:7.41|P-value:6.36E-14|Clearance:0.87||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:BUR6(YER159C)|FD-Score:6.02|P-value:8.81E-10|Clearance:0.25||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CDC1(YDR182W)|FD-Score:3.74|P-value:9.09E-5|Clearance:0.13||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:ERG11(YHR007C)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.23||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GRS1(YBR121C)|FD-Score:-4.39|P-value:5.72E-6|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:5.77|P-value:4.08E-9|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAK21(YDR060W)|FD-Score:-4.98|P-value:3.18E-7|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:NSE5(YML023C)|FD-Score:-3.43|P-value:2.97E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:POP8(YBL018C)|FD-Score:3.31|P-value:4.73E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP3(YDR473C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.03||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RAM2(YKL019W)|FD-Score:5.66|P-value:7.43E-9|Clearance:0.43||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RIB3(YDR487C)|FD-Score:4.08|P-value:2.29E-5|Clearance:0.29||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RIX1(YHR197W)|FD-Score:-3.29|P-value:5.04E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPP1(YHR062C)|FD-Score:-5.99|P-value:1.07E-9|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPR2(YIR015W)|FD-Score:3.1|P-value:9.59E-4|Clearance:0||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPT4(YOR259C)|FD-Score:4.73|P-value:1.12E-6|Clearance:0.08||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RSC8(YFR037C)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SAH1(YER043C)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.12||SGD DESC:S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor Gene:SEC62(YPL094C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.05||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SKP1(YDR328C)|FD-Score:8.27|P-value:6.44E-17|Clearance:0.87||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SPC42(YKL042W)|FD-Score:4.86|P-value:5.79E-7|Clearance:0.13||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPT15(YER148W)|FD-Score:-3.2|P-value:6.81E-4|Clearance:0||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TAF1(YGR274C)|FD-Score:-3.19|P-value:7.07E-4|Clearance:0||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TFA1(YKL028W)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.17||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFB1(YDR311W)|FD-Score:4.65|P-value:1.66E-6|Clearance:0.1||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:YDR187C(YDR187C_d)|FD-Score:3.79|P-value:7.68E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR115C(YGR115C_d)|FD-Score:4.55|P-value:2.74E-6|Clearance:0.3||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJR023C(YJR023C_d)|FD-Score:6.53|P-value:3.18E-11|Clearance:0.52||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:YLL037W(YLL037W_d)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6708777
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ADD37(YMR184W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:ADH5(YBR145W)|FD-Score:6.04|P-value:7.80E-10||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AIM26(YKL037W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AKR1(YDR264C)|FD-Score:-4.54|P-value:2.80E-6||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALG8(YOR067C)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:AMA1(YGR225W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:APL3(YBL037W)|FD-Score:-3.84|P-value:6.06E-5||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:APT2(YDR441C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ARK1(YNL020C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:5.62|P-value:9.69E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ASI1(YMR119W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ASP1(YDR321W)|FD-Score:3.92|P-value:4.51E-5||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:ATG23(YLR431C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BBC1(YJL020C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BDH1(YAL060W)|FD-Score:5.75|P-value:4.52E-9||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BNI1(YNL271C)|FD-Score:-5.48|P-value:2.14E-8||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BNI4(YNL233W)|FD-Score:4.88|P-value:5.33E-7||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BOI1(YBL085W)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BUD2(YKL092C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CMR3(YPR013C_p)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:CPT1(YNL130C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CRP1(YHR146W)|FD-Score:-5.42|P-value:3.06E-8||SGD DESC:Protein that binds to cruciform DNA structures Gene:CST6(YIL036W)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DID4(YKL002W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DOG1(YHR044C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:ECM19(YLR390W)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EMP65(YER140W_p)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERT1(YBR239C_p)|FD-Score:4.34|P-value:6.98E-6||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:EXO5(YBR163W)|FD-Score:-4.53|P-value:2.90E-6||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FIG1(YBR040W)|FD-Score:-5.29|P-value:6.28E-8||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:FIT1(YDR534C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMP25(YLR077W)|FD-Score:5.89|P-value:1.90E-9||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:GAD1(YMR250W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCN3(YKR026C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN5(YGR252W)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GEX2(YKR106W)|FD-Score:5.2|P-value:1.00E-7||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GRR1(YJR090C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTO3(YMR251W)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HMG1(YML075C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOS1(YPR068C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HSP30(YCR021C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:HUA2(YOR284W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HUB1(YNR032C-A)|FD-Score:14.3|P-value:1.58E-46||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:HXT2(YMR011W)|FD-Score:7.96|P-value:8.64E-16||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IMG2(YCR071C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:INO4(YOL108C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IZH1(YDR492W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:KAR4(YCL055W)|FD-Score:-5.38|P-value:3.70E-8||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KAR5(YMR065W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KIP1(YBL063W)|FD-Score:3.74|P-value:9.36E-5||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:LAG1(YHL003C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LSC2(YGR244C)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MDS3(YGL197W)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MIG2(YGL209W)|FD-Score:5.45|P-value:2.49E-8||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MIS1(YBR084W)|FD-Score:-5.1|P-value:1.72E-7||SGD DESC:Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase Gene:MNT2(YGL257C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MPP6(YNR024W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPL16(YBL038W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL4(YLR439W)|FD-Score:-5.23|P-value:8.48E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSE1(YOL033W)|FD-Score:-4.69|P-value:1.36E-6||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MSL1(YIR009W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:MSS51(YLR203C)|FD-Score:5|P-value:2.86E-7||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NTH1(YDR001C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:PCL5(YHR071W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PDR15(YDR406W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PET122(YER153C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PMS1(YNL082W)|FD-Score:-7.17|P-value:3.73E-13||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRE9(YGR135W)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PRM1(YNL279W)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PST2(YDR032C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PUF3(YLL013C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD52(YML032C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RAV2(YDR202C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RFM1(YOR279C)|FD-Score:7.1|P-value:6.40E-13||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RHB1(YCR027C)|FD-Score:-4.32|P-value:7.69E-6||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RIF1(YBR275C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIM1(YCR028C-A)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RNR4(YGR180C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL12A(YEL054C)|FD-Score:5.64|P-value:8.66E-9||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:6.15|P-value:3.97E-10||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL43B(YJR094W-A)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RTS3(YGR161C_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SAP4(YGL229C)|FD-Score:-3.82|P-value:6.74E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCT1(YBL011W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SIC1(YLR079W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SLT2(YHR030C)|FD-Score:3.81|P-value:6.81E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SLX1(YBR228W)|FD-Score:4.51|P-value:3.22E-6||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SPG4(YMR107W)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPT3(YDR392W)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT7(YBR081C)|FD-Score:-3.92|P-value:4.48E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSD1(YDR293C)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSH4(YKL124W)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:SWM1(YDR260C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TAD1(YGL243W)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala Gene:TEX1(YNL253W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TPN1(YGL186C)|FD-Score:4.28|P-value:9.14E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPO2(YGR138C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication Gene:TPO3(YPR156C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TRE1(YPL176C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM732(YMR259C_p)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UPS2(YLR168C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:UTR4(YEL038W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus Gene:VID27(YNL212W)|FD-Score:8.71|P-value:1.51E-18||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:VMA4(YOR332W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS3(YDR495C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBL055C(YBL055C)|FD-Score:5.58|P-value:1.24E-8||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR027C(YBR027C_d)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR200W-A(YBR200W-A_p)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR259W(YBR259W_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YDL129W(YDL129W_p)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL177C(YDL177C_p)|FD-Score:-5.23|P-value:8.48E-8||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDL187C(YDL187C_d)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR018C(YDR018C_p)|FD-Score:5.32|P-value:5.05E-8||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR249C(YDR249C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function Gene:YEH2(YLR020C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YER135C(YER135C_d)|FD-Score:-4.57|P-value:2.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YER156C(YER156C_p)|FD-Score:7.27|P-value:1.86E-13||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YFL040W(YFL040W_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGL109W(YGL109W_d)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YGR045C(YGR045C_d)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR153W(YGR153W_p)|FD-Score:7.62|P-value:1.31E-14||SGD DESC:Putative protein of unknown function Gene:YHR182W(YHR182W_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YJL218W(YJL218W_p)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YJR056C(YJR056C_p)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YJR096W(YJR096W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKE4(YIL023C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL023W(YKL023W_p)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR047W(YKR047W_d)|FD-Score:-5.17|P-value:1.19E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR125W(YLR125W_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YML057C-A(YML057C-A_d)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR018W(YMR018W_p)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR221C(YMR221C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YMR306C-A(YMR306C-A_d)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL162W-A(YNL162W-A_p)|FD-Score:5.43|P-value:2.81E-8||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOL118C(YOL118C_d)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL159C-A(YOL159C-A)|FD-Score:5.26|P-value:7.06E-8||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR296W(YOR296W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YOR366W(YOR366W_d)|FD-Score:-5.89|P-value:1.95E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPR012W(YPR012W_d)|FD-Score:-4.36|P-value:6.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR059C(YPR059C_d)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR077C(YPR077C_d)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT35(YHR105W)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:AAC3(YBR085W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ADD37(YMR184W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:ADH5(YBR145W)|FD-Score:6.04|P-value:7.80E-10||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AIM26(YKL037W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AKR1(YDR264C)|FD-Score:-4.54|P-value:2.80E-6||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALG8(YOR067C)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:AMA1(YGR225W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:APL3(YBL037W)|FD-Score:-3.84|P-value:6.06E-5||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:APT2(YDR441C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ARK1(YNL020C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:5.62|P-value:9.69E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ASI1(YMR119W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ASP1(YDR321W)|FD-Score:3.92|P-value:4.51E-5||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:ATG23(YLR431C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BBC1(YJL020C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BDH1(YAL060W)|FD-Score:5.75|P-value:4.52E-9||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BNI1(YNL271C)|FD-Score:-5.48|P-value:2.14E-8||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BNI4(YNL233W)|FD-Score:4.88|P-value:5.33E-7||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BOI1(YBL085W)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BUD2(YKL092C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CMR3(YPR013C_p)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:CPT1(YNL130C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CRP1(YHR146W)|FD-Score:-5.42|P-value:3.06E-8||SGD DESC:Protein that binds to cruciform DNA structures Gene:CST6(YIL036W)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DID4(YKL002W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DOG1(YHR044C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:ECM19(YLR390W)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EMP65(YER140W_p)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERT1(YBR239C_p)|FD-Score:4.34|P-value:6.98E-6||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:EXO5(YBR163W)|FD-Score:-4.53|P-value:2.90E-6||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FIG1(YBR040W)|FD-Score:-5.29|P-value:6.28E-8||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:FIT1(YDR534C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMP25(YLR077W)|FD-Score:5.89|P-value:1.90E-9||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:GAD1(YMR250W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCN3(YKR026C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN5(YGR252W)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GEX2(YKR106W)|FD-Score:5.2|P-value:1.00E-7||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GRR1(YJR090C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTO3(YMR251W)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HMG1(YML075C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOS1(YPR068C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HSP30(YCR021C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:HUA2(YOR284W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HUB1(YNR032C-A)|FD-Score:14.3|P-value:1.58E-46||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:HXT2(YMR011W)|FD-Score:7.96|P-value:8.64E-16||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IMG2(YCR071C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:INO4(YOL108C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IZH1(YDR492W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:KAR4(YCL055W)|FD-Score:-5.38|P-value:3.70E-8||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KAR5(YMR065W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KIP1(YBL063W)|FD-Score:3.74|P-value:9.36E-5||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:LAG1(YHL003C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LSC2(YGR244C)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MDS3(YGL197W)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MIG2(YGL209W)|FD-Score:5.45|P-value:2.49E-8||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MIS1(YBR084W)|FD-Score:-5.1|P-value:1.72E-7||SGD DESC:Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase Gene:MNT2(YGL257C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MPP6(YNR024W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPL16(YBL038W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL4(YLR439W)|FD-Score:-5.23|P-value:8.48E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSE1(YOL033W)|FD-Score:-4.69|P-value:1.36E-6||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MSL1(YIR009W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:MSS51(YLR203C)|FD-Score:5|P-value:2.86E-7||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NTH1(YDR001C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:PCL5(YHR071W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PDR15(YDR406W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PET122(YER153C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PMS1(YNL082W)|FD-Score:-7.17|P-value:3.73E-13||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRE9(YGR135W)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PRM1(YNL279W)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PST2(YDR032C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PUF3(YLL013C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD52(YML032C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RAV2(YDR202C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RFM1(YOR279C)|FD-Score:7.1|P-value:6.40E-13||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RHB1(YCR027C)|FD-Score:-4.32|P-value:7.69E-6||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RIF1(YBR275C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIM1(YCR028C-A)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RNR4(YGR180C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL12A(YEL054C)|FD-Score:5.64|P-value:8.66E-9||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:6.15|P-value:3.97E-10||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL43B(YJR094W-A)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RTS3(YGR161C_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SAP4(YGL229C)|FD-Score:-3.82|P-value:6.74E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCT1(YBL011W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SIC1(YLR079W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SLT2(YHR030C)|FD-Score:3.81|P-value:6.81E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SLX1(YBR228W)|FD-Score:4.51|P-value:3.22E-6||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SPG4(YMR107W)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPT3(YDR392W)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT7(YBR081C)|FD-Score:-3.92|P-value:4.48E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSD1(YDR293C)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSH4(YKL124W)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:SWM1(YDR260C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TAD1(YGL243W)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala Gene:TEX1(YNL253W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TPN1(YGL186C)|FD-Score:4.28|P-value:9.14E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPO2(YGR138C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication Gene:TPO3(YPR156C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TRE1(YPL176C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM732(YMR259C_p)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UPS2(YLR168C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:UTR4(YEL038W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus Gene:VID27(YNL212W)|FD-Score:8.71|P-value:1.51E-18||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:VMA4(YOR332W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS3(YDR495C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBL055C(YBL055C)|FD-Score:5.58|P-value:1.24E-8||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR027C(YBR027C_d)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR200W-A(YBR200W-A_p)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR259W(YBR259W_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YDL129W(YDL129W_p)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL177C(YDL177C_p)|FD-Score:-5.23|P-value:8.48E-8||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDL187C(YDL187C_d)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR018C(YDR018C_p)|FD-Score:5.32|P-value:5.05E-8||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR249C(YDR249C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function Gene:YEH2(YLR020C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YER135C(YER135C_d)|FD-Score:-4.57|P-value:2.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YER156C(YER156C_p)|FD-Score:7.27|P-value:1.86E-13||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YFL040W(YFL040W_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGL109W(YGL109W_d)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YGR045C(YGR045C_d)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR153W(YGR153W_p)|FD-Score:7.62|P-value:1.31E-14||SGD DESC:Putative protein of unknown function Gene:YHR182W(YHR182W_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YJL218W(YJL218W_p)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YJR056C(YJR056C_p)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YJR096W(YJR096W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKE4(YIL023C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL023W(YKL023W_p)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR047W(YKR047W_d)|FD-Score:-5.17|P-value:1.19E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR125W(YLR125W_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YML057C-A(YML057C-A_d)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR018W(YMR018W_p)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR221C(YMR221C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YMR306C-A(YMR306C-A_d)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL162W-A(YNL162W-A_p)|FD-Score:5.43|P-value:2.81E-8||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOL118C(YOL118C_d)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL159C-A(YOL159C-A)|FD-Score:5.26|P-value:7.06E-8||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR296W(YOR296W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YOR366W(YOR366W_d)|FD-Score:-5.89|P-value:1.95E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPR012W(YPR012W_d)|FD-Score:-4.36|P-value:6.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR059C(YPR059C_d)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR077C(YPR077C_d)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT35(YHR105W)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR328C8.276.44E-170.87SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YDR299W7.416.36E-140.87BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YJR023C_d6.533.18E-110.52YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YER159C6.028.81E-100.25BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YER112W5.774.08E-90.10LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YKL019W5.667.43E-90.43RAM2Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
YER036C5.238.30E-80.37ARB1ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
YKL042W4.865.79E-70.13SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YOR259C4.731.12E-60.08RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YDR311W4.651.66E-60.10TFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
YGR115C_d4.552.74E-60.30YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YKL028W4.241.10E-50.17TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YDR487C4.082.29E-50.29RIB33,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
YDR187C_d3.797.68E-50.04YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YDR182W3.749.09E-50.13CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNR032C-A14.301.58E-46HUB1Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
YNL212W8.711.51E-18VID27Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
YMR011W7.968.64E-16HXT2High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose
YGR153W_p7.621.31E-14YGR153W_pPutative protein of unknown function
YER156C_p7.271.86E-13YER156C_pPutative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1
YOR279C7.106.40E-13RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YIL018W6.153.97E-10RPL2BRibosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures
YBR145W6.047.80E-10ADH5Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
YLR077W5.891.90E-9FMP25Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria
YGR135W5.783.70E-9PRE9Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
YAL060W5.754.52E-9BDH1NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
YEL054C5.648.66E-9RPL12ARibosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
YDR127W5.629.69E-9ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YBL055C5.581.24E-8YBL055C3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
YMR251W5.511.77E-8GTO3Omega class glutathione transferase; putative cytosolic localization

GO enrichment analysis for SGTC_2563
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0758.23E-9SGTC_14274048-4900 550.0 nMChemDiv (Drug-like library)241325860.0377358
0.0663.39E-7SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.113208
0.0656.01E-7SGTC_29329016711 44.5 μMChembridge (Drug-like library)71173280.0252101
0.0647.53E-7SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.256881
0.0596.23E-6SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.025
0.0587.27E-6SGTC_1988st071872 53.4 μMTimTec (Natural product derivative library)164141160.0542636
0.0589.89E-6SGTC_659k018-0002 107.0 μMChemDiv (Drug-like library)67978790.00746269
0.0571.16E-5SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0252101
0.0561.55E-5SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.016129
0.0561.98E-5SGTC_21325317594 122.7 μMChembridge (Fragment library)7945770.00869565SWF1 & branched chain AA biosynthesis
0.0543.78E-5SGTC_28549024559 71.4 μMChembridge (Drug-like library)31635290.0314961
0.0535.00E-5SGTC_2564betulinic acid 100.0 μMMicrosource (Natural product library)67085170.142857
0.0525.94E-5SGTC_185k048-0037 25.5 μMChemDiv (Drug-like library)68275890.00833333RSC & ERG11
0.0518.51E-5SGTC_6464092-0839 27.3 μMChemDiv (Drug-like library)8777960.0373832plasma membrane duress
0.0518.80E-5SGTC_5423381-0244 89.3 μMChemDiv (Drug-like library)11128430.0336134

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1568digitonin820 nM0.4954952735010TimTec (Pure natural product library)1229.31228-5.2071729sphingolipid biosynthesis & PDR1
SGTC_2605digitonin300 nM0.4954952735010TimTec (Pure natural product library)1229.31228-5.2071729
SGTC_266formosanin C406 nM0.36363673597Miscellaneous1015.184780.311020sphingolipid biosynthesis & PDR1
SGTC_2572astragaloside iv100 μM0.27642345006101Microsource (Natural product library)784.97022-0.348914
SGTC_418lividomycin a100 μM0.2745172394Miscellaneous761.7697-9.8431523
SGTC_2607solasodine47.35 μM0.2568816710642Microsource (Natural product library)413.635824.36723
SGTC_2667digitoxin100 μM0.255702041Microsource (Natural product library)764.939063.101513
SGTC_401neomycin50 μM0.2474238378Miscellaneous614.64374-8.9591319
SGTC_2688kanamycin b96.69 μM0.22449636396Microsource (Natural product library)581.59236-8.041319calcium & mitochondrial duress
SGTC_1572epiandrosterone68.9 μM0.216495441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.216495441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.2164952754130Microsource (Natural product library)290.44033.58812cell wall