betulinic acid

(1R,3aS,5bS,9R,11aR,13aS)-9-hydroxy-5b,8,8,11a-tetramethyl-1-prop-1-en-2-yl-2,3,4,5,5a,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydro-1H-cyclopenta[a]chrysene-3a-carboxylic acid

Apoptosis-inducing agent of unknown mechanism.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2564
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 6708517
SMILES CC(=C)C1CCC2(C1C3CCC4C(C3CC2)(CCC5C4(CCC(C5(C)C)O)C)C)C(=O)O
Standardized SMILES CC(=C)C1CCC2(CCC3C(CCC4C3(C)CCC5C(C)(C)C(O)CCC45C)C12)C(=O)O
Molecular weight 442.6737
ALogP 6.34
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.54
% growth inhibition (Hom. pool) -0.01


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6708517
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:13.7|P-value:5.10E-43|Clearance:7.63||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:AME1(YBR211C)|FD-Score:5.36|P-value:4.22E-8|Clearance:0.24||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:BFR2(YDR299W)|FD-Score:5|P-value:2.89E-7|Clearance:0.6||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:CLF1(YLR117C)|FD-Score:-3.57|P-value:1.82E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:CUS1(YMR240C)|FD-Score:5.12|P-value:1.56E-7|Clearance:0.07||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:IRR1(YIL026C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.01||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KEI1(YDR367W)|FD-Score:3.8|P-value:7.11E-5|Clearance:0.07||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MCM3(YEL032W)|FD-Score:-4.01|P-value:3.10E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MNP1(YGL068W)|FD-Score:3.88|P-value:5.17E-5|Clearance:0.08||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:MRPS18(YNL306W)|FD-Score:-3.15|P-value:8.27E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NAB2(YGL122C)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.24||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NAF1(YNL124W)|FD-Score:6.07|P-value:6.32E-10|Clearance:0.71||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:RFA3(YJL173C)|FD-Score:-5.32|P-value:5.17E-8|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RSC4(YKR008W)|FD-Score:4.4|P-value:5.34E-6|Clearance:0.52||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC6(YCR052W)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SAM50(YNL026W)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.21||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SMX3(YPR182W)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.05||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:VHT1(YGR065C)|FD-Score:-7.88|P-value:1.64E-15|Clearance:0||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:WBP1(YEL002C)|FD-Score:5.04|P-value:2.28E-7|Clearance:0.05||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YBR089W(YBR089W_d)|FD-Score:3.23|P-value:6.16E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:ALG14(YBR070C)|FD-Score:13.7|P-value:5.10E-43|Clearance:7.63||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:AME1(YBR211C)|FD-Score:5.36|P-value:4.22E-8|Clearance:0.24||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:BFR2(YDR299W)|FD-Score:5|P-value:2.89E-7|Clearance:0.6||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:CLF1(YLR117C)|FD-Score:-3.57|P-value:1.82E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:CUS1(YMR240C)|FD-Score:5.12|P-value:1.56E-7|Clearance:0.07||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:IRR1(YIL026C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.01||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KEI1(YDR367W)|FD-Score:3.8|P-value:7.11E-5|Clearance:0.07||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MCM3(YEL032W)|FD-Score:-4.01|P-value:3.10E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MNP1(YGL068W)|FD-Score:3.88|P-value:5.17E-5|Clearance:0.08||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:MRPS18(YNL306W)|FD-Score:-3.15|P-value:8.27E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NAB2(YGL122C)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.24||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NAF1(YNL124W)|FD-Score:6.07|P-value:6.32E-10|Clearance:0.71||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:RFA3(YJL173C)|FD-Score:-5.32|P-value:5.17E-8|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RSC4(YKR008W)|FD-Score:4.4|P-value:5.34E-6|Clearance:0.52||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC6(YCR052W)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SAM50(YNL026W)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.21||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SMX3(YPR182W)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.05||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:VHT1(YGR065C)|FD-Score:-7.88|P-value:1.64E-15|Clearance:0||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:WBP1(YEL002C)|FD-Score:5.04|P-value:2.28E-7|Clearance:0.05||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YBR089W(YBR089W_d)|FD-Score:3.23|P-value:6.16E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6708517
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ABP1(YCR088W)|FD-Score:10.5|P-value:3.49E-26||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:ADH7(YCR105W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:AGP3(YFL055W)|FD-Score:4.79|P-value:8.15E-7||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:AHA1(YDR214W)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AIM3(YBR108W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARG82(YDR173C)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:BMH1(YER177W)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BUD25(YER014C-A)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:BUD30(YDL151C_d)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAD1(YDR423C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CAP2(YIL034C)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CDC55(YGL190C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CIS3(YJL158C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:CKB2(YOR039W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNE1(YAL058W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CPR1(YDR155C)|FD-Score:6.53|P-value:3.32E-11||SGD DESC:Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress Gene:CST9(YLR394W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DCS1(YLR270W)|FD-Score:-3.73|P-value:9.50E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DMA1(YHR115C)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:ECM7(YLR443W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EFT1(YOR133W)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:EMI1(YDR512C)|FD-Score:5.98|P-value:1.10E-9||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG5(YMR015C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV15(YBR210W)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FAA2(YER015W)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FIG2(YCR089W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FMO1(YHR176W)|FD-Score:-5.46|P-value:2.33E-8||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP25(YLR077W)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FYV4(YHR059W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV5(YCL058C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:FYV6(YNL133C)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GLG2(YJL137C)|FD-Score:4.44|P-value:4.49E-6||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HSL1(YKL101W)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:IMG1(YCR046C)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IMG2(YCR071C)|FD-Score:-4.35|P-value:6.73E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IMP1(YMR150C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:INO4(YOL108C)|FD-Score:-4.45|P-value:4.28E-6||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IPT1(YDR072C)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KIP1(YBL063W)|FD-Score:6.53|P-value:3.22E-11||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:KNS1(YLL019C)|FD-Score:8.15|P-value:1.90E-16||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LDB19(YOR322C)|FD-Score:7.36|P-value:9.45E-14||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LDB7(YBL006C)|FD-Score:4.79|P-value:8.21E-7||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LOC1(YFR001W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAK32(YCR019W)|FD-Score:-4.01|P-value:2.98E-5||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MAP2(YBL091C)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MET32(YDR253C)|FD-Score:4.68|P-value:1.47E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MMT2(YPL224C)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MRPL31(YKL138C)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTC7(YEL033W_p)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MUB1(YMR100W)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NAM8(YHR086W)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NGL1(YOL042W)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PDC6(YGR087C)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PET122(YER153C)|FD-Score:-8.89|P-value:2.92E-19||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PHB2(YGR231C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PMS1(YNL082W)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PSR1(YLL010C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PTH2(YBL057C)|FD-Score:5.87|P-value:2.24E-9||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUF6(YDR496C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RAD16(YBR114W)|FD-Score:5.21|P-value:9.64E-8||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RPE1(YJL121C)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL11B(YGR085C)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-5.54|P-value:1.53E-8||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RSR1(YGR152C)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:RTT10(YPL183C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SEY1(YOR165W)|FD-Score:-3.83|P-value:6.50E-5||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFA1(YDL168W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SGO1(YOR073W)|FD-Score:-5.67|P-value:7.35E-9||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHE2(YKL130C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SHS1(YDL225W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SIP1(YDR422C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SLH1(YGR271W)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLI15(YBR156C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SLO1(YER180C-A)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SPA2(YLL021W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPC72(YAL047C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SSF2(YDR312W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:SYT1(YPR095C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TEP1(YNL128W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:THP3(YPR045C)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TPM2(YIL138C)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRK1(YJL129C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM13(YOL125W)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:UBC11(YOR339C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UBP3(YER151C)|FD-Score:4.83|P-value:6.73E-7||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:ULI1(YFR026C)|FD-Score:-3.88|P-value:5.26E-5||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:ULS1(YOR191W)|FD-Score:-5.16|P-value:1.22E-7||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UNG1(YML021C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:UTR4(YEL038W)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus Gene:VID28(YIL017C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPS21(YOR089C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBL055C(YBL055C)|FD-Score:4.99|P-value:2.97E-7||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YDL114W(YDL114W_p)|FD-Score:4.39|P-value:5.64E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YDR015C(YDR015C_d)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR018C(YDR018C_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR109C(YDR109C_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Putative kinase Gene:YDR124W(YDR124W_p)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YGL132W(YGL132W_d)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YGL217C(YGL217C_d)|FD-Score:6.89|P-value:2.72E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGL260W(YGL260W_p)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR125W(YHR125W_d)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR035C(YIR035C_p)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL150W(YJL150W_d)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR070W(YKR070W_p)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR224W(YLR224W_p)|FD-Score:-4.98|P-value:3.17E-7||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR252C(YMR252C_p)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YOL159C-A(YOL159C-A)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR302W(YOR302W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA Gene:YPL073C(YPL073C_d)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPR126C(YPR126C_d)|FD-Score:-5.61|P-value:1.02E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AAC3(YBR085W)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ABP1(YCR088W)|FD-Score:10.5|P-value:3.49E-26||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:ADH7(YCR105W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:AGP3(YFL055W)|FD-Score:4.79|P-value:8.15E-7||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:AHA1(YDR214W)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AIM3(YBR108W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARG82(YDR173C)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:BMH1(YER177W)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BUD25(YER014C-A)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:BUD30(YDL151C_d)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAD1(YDR423C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CAP2(YIL034C)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CDC55(YGL190C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CIS3(YJL158C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:CKB2(YOR039W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNE1(YAL058W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CPR1(YDR155C)|FD-Score:6.53|P-value:3.32E-11||SGD DESC:Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress Gene:CST9(YLR394W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DCS1(YLR270W)|FD-Score:-3.73|P-value:9.50E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DMA1(YHR115C)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:ECM7(YLR443W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EFT1(YOR133W)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:EMI1(YDR512C)|FD-Score:5.98|P-value:1.10E-9||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG5(YMR015C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV15(YBR210W)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FAA2(YER015W)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FIG2(YCR089W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FMO1(YHR176W)|FD-Score:-5.46|P-value:2.33E-8||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP25(YLR077W)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FYV4(YHR059W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV5(YCL058C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:FYV6(YNL133C)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GLG2(YJL137C)|FD-Score:4.44|P-value:4.49E-6||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HSL1(YKL101W)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:IMG1(YCR046C)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IMG2(YCR071C)|FD-Score:-4.35|P-value:6.73E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IMP1(YMR150C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:INO4(YOL108C)|FD-Score:-4.45|P-value:4.28E-6||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IPT1(YDR072C)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KIP1(YBL063W)|FD-Score:6.53|P-value:3.22E-11||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:KNS1(YLL019C)|FD-Score:8.15|P-value:1.90E-16||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LDB19(YOR322C)|FD-Score:7.36|P-value:9.45E-14||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LDB7(YBL006C)|FD-Score:4.79|P-value:8.21E-7||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LOC1(YFR001W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAK32(YCR019W)|FD-Score:-4.01|P-value:2.98E-5||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MAP2(YBL091C)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MET32(YDR253C)|FD-Score:4.68|P-value:1.47E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MMT2(YPL224C)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MRPL31(YKL138C)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTC7(YEL033W_p)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MUB1(YMR100W)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NAM8(YHR086W)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NGL1(YOL042W)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PDC6(YGR087C)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PET122(YER153C)|FD-Score:-8.89|P-value:2.92E-19||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PHB2(YGR231C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PMS1(YNL082W)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PSR1(YLL010C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PTH2(YBL057C)|FD-Score:5.87|P-value:2.24E-9||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUF6(YDR496C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RAD16(YBR114W)|FD-Score:5.21|P-value:9.64E-8||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RPE1(YJL121C)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL11B(YGR085C)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-5.54|P-value:1.53E-8||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RSR1(YGR152C)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:RTT10(YPL183C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SEY1(YOR165W)|FD-Score:-3.83|P-value:6.50E-5||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFA1(YDL168W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SGO1(YOR073W)|FD-Score:-5.67|P-value:7.35E-9||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHE2(YKL130C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SHS1(YDL225W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SIP1(YDR422C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SLH1(YGR271W)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLI15(YBR156C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SLO1(YER180C-A)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SPA2(YLL021W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPC72(YAL047C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SSF2(YDR312W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:SYT1(YPR095C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TEP1(YNL128W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:THP3(YPR045C)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TPM2(YIL138C)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRK1(YJL129C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM13(YOL125W)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:UBC11(YOR339C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UBP3(YER151C)|FD-Score:4.83|P-value:6.73E-7||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:ULI1(YFR026C)|FD-Score:-3.88|P-value:5.26E-5||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:ULS1(YOR191W)|FD-Score:-5.16|P-value:1.22E-7||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UNG1(YML021C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:UTR4(YEL038W)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus Gene:VID28(YIL017C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPS21(YOR089C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBL055C(YBL055C)|FD-Score:4.99|P-value:2.97E-7||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YDL114W(YDL114W_p)|FD-Score:4.39|P-value:5.64E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YDR015C(YDR015C_d)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR018C(YDR018C_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR109C(YDR109C_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Putative kinase Gene:YDR124W(YDR124W_p)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YGL132W(YGL132W_d)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YGL217C(YGL217C_d)|FD-Score:6.89|P-value:2.72E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGL260W(YGL260W_p)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR125W(YHR125W_d)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR035C(YIR035C_p)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL150W(YJL150W_d)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR070W(YKR070W_p)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR224W(YLR224W_p)|FD-Score:-4.98|P-value:3.17E-7||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR252C(YMR252C_p)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YOL159C-A(YOL159C-A)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR302W(YOR302W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA Gene:YPL073C(YPL073C_d)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPR126C(YPR126C_d)|FD-Score:-5.61|P-value:1.02E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YBR070C13.705.10E-437.63ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YNL124W6.076.32E-100.71NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YBR211C5.364.22E-80.24AME1Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
YMR240C5.121.56E-70.07CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YEL002C5.042.28E-70.05WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
YDR299W5.002.89E-70.60BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YKR008W4.405.34E-60.52RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
YGL068W3.885.17E-50.08MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YDR367W3.807.11E-50.07KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YNL026W3.749.28E-50.21SAM50Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
YPR182W3.522.13E-40.05SMX3Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
YIL026C3.482.53E-40.01IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YGL122C3.472.60E-40.24NAB2Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress
YBR089W_d3.236.16E-40.16YBR089W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
YMR298W3.070.001070.03LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR088W10.503.49E-26ABP1Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
YLL019C8.151.90E-16KNS1Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
YOR322C7.369.45E-14LDB19Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye
YGL217C_d6.892.72E-12YGL217C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W
YBL063W6.533.22E-11KIP1Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
YDR155C6.533.32E-11CPR1Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress
YDR512C5.981.10E-9EMI1Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs
YBL057C5.872.24E-9PTH2One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth
YDR214W5.422.94E-8AHA1Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
YPR045C5.403.35E-8THP3Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
YBR114W5.219.64E-8RAD16Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
YBR085W5.081.93E-7AAC3Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
YBL055C4.992.97E-7YBL055C3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
YER151C4.836.73E-7UBP3Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
YOR133W4.808.13E-7EFT1Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2564
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0657.09E-7SGTC_1784st039334 36.3 μMTimTec (Natural product derivative library)34969840.027027
0.0596.35E-6SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.047619
0.0589.60E-6SGTC_482fipronil 114.0 μMICCB bioactive library33520.0111111
0.0534.16E-5SGTC_2567aureobasidin a 60.0 nMMiscellaneous99634300.047619
0.0535.00E-5SGTC_2563tomatine 8.5 μMMicrosource (Natural product library)67087770.142857
0.0511.04E-4SGTC_1893970-0742 2.1 μMChemDiv (Drug-like library)28918070.012987
0.0501.10E-4SGTC_12961068-0102 11.9 μMChemDiv (Drug-like library)7670530.037037
0.0501.38E-4SGTC_2762leflunomide 42.1 μMMiscellaneous38990.05
0.0491.78E-4SGTC_650798-0905 35.0 μMChemDiv (Drug-like library)36021450.0319149calcium & mitochondrial duress
0.0455.47E-4SGTC_2537larixol 68.9 μMMicrosource (Natural product library)67087590.123457
0.0448.10E-4SGTC_1616st002734 56.1 μMTimTec (Natural product derivative library)40261010.0882353
0.0448.41E-4SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.0560S ribosome export
0.0420.00125SGTC_1763st044482 94.5 μMTimTec (Natural product derivative library)885050.0273973copper-dependent oxidative stress
0.0400.00189SGTC_14514152-0210 104.0 μMChemDiv (Drug-like library)659563NA
0.0390.00300SGTC_7381315-0003 72.4 μMChemDiv (Drug-like library)62514740.0102041

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1108betulinic acid729.14 nM0.38356264945TimTec (Natural product library)456.700326.49223
SGTC_1557sumaresinolic acid42.3 μM0.2948729982268TimTec (Pure natural product library)472.699725.27734
SGTC_1560lithocholic acid53.1 μM0.270279903TimTec (Pure natural product library)376.57265.18423
SGTC_1572epiandrosterone68.9 μM0.246377441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.246377441302TimTec (Pure natural product library)290.44033.58812
SGTC_1974st06944826.9 μM0.2463776323587TimTec (Natural product derivative library)305.454943.36923
SGTC_2584androsterone48.74 μM0.2463772754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_1127asiatic acid681.4 nM0.229885470603TimTec (Natural product library)488.699124.43545
SGTC_2583asiatic acid100 μM0.2298855320556Microsource (Natural product library)488.699124.43545
SGTC_2687chenodiol100 μM0.2179495702181Microsource (Natural product library)392.5724.01434
SGTC_417st075190100 μM0.216867222757TimTec (Pure natural product library)376.488025.51213
SGTC_1517deoxycholic acid methyl ester49.2 μM0.216867229346TimTec (Pure natural product library)406.598584.30724