2',4'-dihydroxy-4-methoxychalcone

(E)-1-(2,4-dihydroxyphenyl)-3-(4-methoxyphenyl)prop-2-en-1-one

A potent inhibitor of glutathione S-transferases.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2577
Screen concentration 15.3 μM
Source Microsource (Natural product library)
PubChem CID 5711223
SMILES COC1=CC=C(C=C1)C=CC(=O)C2=C(C=C(C=C2)O)O
Standardized SMILES COc1ccc(C=CC(=O)c2ccc(O)cc2O)cc1
Molecular weight 270.28
ALogP 3.2
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.18
% growth inhibition (Hom. pool) 12.84


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5711223
Download HIP data (tab-delimited text)  (excel)
Gene:AOS1(YPR180W)|FD-Score:-3.73|P-value:9.58E-5|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:CCT4(YDL143W)|FD-Score:4.47|P-value:3.94E-6|Clearance:0.4||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC37(YDR168W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.19||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:CSE4(YKL049C)|FD-Score:-3.36|P-value:3.84E-4|Clearance:0||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:ERG9(YHR190W)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:HRR25(YPL204W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MCD4(YKL165C)|FD-Score:3.94|P-value:4.05E-5|Clearance:0.05||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM6(YGL201C)|FD-Score:8.22|P-value:1.03E-16|Clearance:3.75||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MEC1(YBR136W)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.01||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NIP7(YPL211W)|FD-Score:3.13|P-value:8.60E-4|Clearance:0.04||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NUP1(YOR098C)|FD-Score:-3.73|P-value:9.54E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:RPT3(YDR394W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:SEC31(YDL195W)|FD-Score:3.23|P-value:6.12E-4|Clearance:0.1||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEN1(YLR430W)|FD-Score:4.07|P-value:2.32E-5|Clearance:0.13||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:TIM50(YPL063W)|FD-Score:-3.96|P-value:3.81E-5|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:VAS1(YGR094W)|FD-Score:3.89|P-value:4.97E-5|Clearance:0.41||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YDR187C(YDR187C_d)|FD-Score:3.09|P-value:9.94E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:AOS1(YPR180W)|FD-Score:-3.73|P-value:9.58E-5|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:CCT4(YDL143W)|FD-Score:4.47|P-value:3.94E-6|Clearance:0.4||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC37(YDR168W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.19||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:CSE4(YKL049C)|FD-Score:-3.36|P-value:3.84E-4|Clearance:0||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:ERG9(YHR190W)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:HRR25(YPL204W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MCD4(YKL165C)|FD-Score:3.94|P-value:4.05E-5|Clearance:0.05||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM6(YGL201C)|FD-Score:8.22|P-value:1.03E-16|Clearance:3.75||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MEC1(YBR136W)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.01||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NIP7(YPL211W)|FD-Score:3.13|P-value:8.60E-4|Clearance:0.04||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NUP1(YOR098C)|FD-Score:-3.73|P-value:9.54E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:RPT3(YDR394W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:SEC31(YDL195W)|FD-Score:3.23|P-value:6.12E-4|Clearance:0.1||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEN1(YLR430W)|FD-Score:4.07|P-value:2.32E-5|Clearance:0.13||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:TIM50(YPL063W)|FD-Score:-3.96|P-value:3.81E-5|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:VAS1(YGR094W)|FD-Score:3.89|P-value:4.97E-5|Clearance:0.41||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YDR187C(YDR187C_d)|FD-Score:3.09|P-value:9.94E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5711223
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ABZ2(YMR289W)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ARI1(YGL157W)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:AVT7(YIL088C)|FD-Score:-6.2|P-value:2.83E-10||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CCW14(YLR390W-A)|FD-Score:-8.37|P-value:2.90E-17||SGD DESC:Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall Gene:CHL1(YPL008W)|FD-Score:4.3|P-value:8.66E-6||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:CHO1(YER026C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:COG7(YGL005C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX10(YPL172C)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:COX18(YGR062C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:COX19(YLL018C-A)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:COX9(YDL067C)|FD-Score:4.33|P-value:7.47E-6||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTH1(YDR151C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:DAL81(YIR023W)|FD-Score:12.1|P-value:4.70E-34||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ECM22(YLR228C)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ENO1(YGR254W)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERV14(YGL054C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ERV25(YML012W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FLO1(YAR050W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FMP27(YLR454W_p)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV1(YDR024W_d)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:FYV6(YNL133C)|FD-Score:5.51|P-value:1.82E-8||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAS3(YMR215W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GAT3(YLR013W)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDH3(YAL062W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GET1(YGL020C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GEX2(YKR106W)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GIT1(YCR098C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:HFA1(YMR207C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HFI1(YPL254W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HSM3(YBR272C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HUL4(YJR036C)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IML2(YJL082W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:9.15|P-value:2.83E-20||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISU2(YOR226C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:JHD2(YJR119C)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:LCB5(YLR260W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEO1(YOR123C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LHS1(YKL073W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MCR1(YKL150W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MHF1(YOL086W-A)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MRPL35(YDR322W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC3(YLR219W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:NOP13(YNL175C)|FD-Score:3.85|P-value:5.93E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NRG2(YBR066C)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OLA1(YBR025C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:PGM2(YMR105C)|FD-Score:-3.85|P-value:6.02E-5||SGD DESC:Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress Gene:PHB1(YGR132C)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PHO88(YBR106W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:REF2(YDR195W)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RFS1(YBR052C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPN4(YDL020C)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC1(YOL138C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RTS3(YGR161C_p)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:RXT2(YBR095C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SEE1(YIL064W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SET6(YPL165C)|FD-Score:-5.39|P-value:3.50E-8||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SHE2(YKL130C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SHO1(YER118C)|FD-Score:-5.37|P-value:3.92E-8||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SIN4(YNL236W)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKT5(YBL061C)|FD-Score:5.36|P-value:4.18E-8||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLF1(YDR515W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SLS1(YLR139C)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMK1(YPR054W)|FD-Score:-5.06|P-value:2.07E-7||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SPT21(YMR179W)|FD-Score:5.67|P-value:7.30E-9||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT4(YGR063C)|FD-Score:5.57|P-value:1.30E-8||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUR4(YLR372W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYH1(YPL105C)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TFB5(YDR079C-A)|FD-Score:5.22|P-value:9.00E-8||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TGS1(YPL157W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TIR3(YIL011W)|FD-Score:-4.96|P-value:3.44E-7||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TLG2(YOL018C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPK1(YJL164C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:9.86|P-value:3.13E-23||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.85|P-value:3.62E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.53|P-value:3.33E-11||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TYW1(YPL207W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:VMA4(YOR332W)|FD-Score:4.48|P-value:3.80E-6||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS52(YDR484W)|FD-Score:6.25|P-value:2.10E-10||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL008W-A(YBL008W-A_p)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL029C-A(YBL029C-A_p)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBR071W(YBR071W)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YDR008C(YDR008C_d)|FD-Score:6.67|P-value:1.28E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR282C(YDR282C_p)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YDR340W(YDR340W_d)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Putative protein of unknown function Gene:YDR455C(YDR455C_d)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGL015C(YGL015C_p)|FD-Score:4.62|P-value:1.89E-6||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGL041C(YGL041C_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL088W(YGL088W_d)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR125W(YGR125W_p)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHI9(YHR029C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHR007C-A(YHR007C-A_p)|FD-Score:-3.95|P-value:3.92E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR177W(YHR177W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIL163C(YIL163C_p)|FD-Score:-5.19|P-value:1.02E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YIR007W(YIR007W_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YLR042C(YLR042C_p)|FD-Score:-4.06|P-value:2.50E-5||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR269C(YLR269C_d)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.86|P-value:5.89E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL089C(YNL089C_d)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNL184C(YNL184C_p)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL277W-A(YNL277W-A_p)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function Gene:YOR022C(YOR022C_p)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPK1(YKL126W)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPK3(YBR028C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL035C(YPL035C_d)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:ZRT1(YGL255W)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:AAP1(YHR047C)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ABZ2(YMR289W)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ARI1(YGL157W)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:AVT7(YIL088C)|FD-Score:-6.2|P-value:2.83E-10||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CCW14(YLR390W-A)|FD-Score:-8.37|P-value:2.90E-17||SGD DESC:Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall Gene:CHL1(YPL008W)|FD-Score:4.3|P-value:8.66E-6||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:CHO1(YER026C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:COG7(YGL005C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX10(YPL172C)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:COX18(YGR062C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:COX19(YLL018C-A)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:COX9(YDL067C)|FD-Score:4.33|P-value:7.47E-6||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTH1(YDR151C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:DAL81(YIR023W)|FD-Score:12.1|P-value:4.70E-34||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ECM22(YLR228C)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ENO1(YGR254W)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERV14(YGL054C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ERV25(YML012W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FLO1(YAR050W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FMP27(YLR454W_p)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV1(YDR024W_d)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:FYV6(YNL133C)|FD-Score:5.51|P-value:1.82E-8||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAS3(YMR215W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GAT3(YLR013W)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDH3(YAL062W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GET1(YGL020C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GEX2(YKR106W)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GIT1(YCR098C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:HFA1(YMR207C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HFI1(YPL254W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HSM3(YBR272C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HUL4(YJR036C)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IML2(YJL082W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:9.15|P-value:2.83E-20||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISU2(YOR226C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:JHD2(YJR119C)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:LCB5(YLR260W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEO1(YOR123C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LHS1(YKL073W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MCR1(YKL150W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MHF1(YOL086W-A)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MRPL35(YDR322W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC3(YLR219W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:NOP13(YNL175C)|FD-Score:3.85|P-value:5.93E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NRG2(YBR066C)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OLA1(YBR025C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:PGM2(YMR105C)|FD-Score:-3.85|P-value:6.02E-5||SGD DESC:Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress Gene:PHB1(YGR132C)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PHO88(YBR106W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:REF2(YDR195W)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RFS1(YBR052C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPN4(YDL020C)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC1(YOL138C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RTS3(YGR161C_p)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:RXT2(YBR095C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SEE1(YIL064W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SET6(YPL165C)|FD-Score:-5.39|P-value:3.50E-8||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SHE2(YKL130C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SHO1(YER118C)|FD-Score:-5.37|P-value:3.92E-8||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SIN4(YNL236W)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKT5(YBL061C)|FD-Score:5.36|P-value:4.18E-8||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLF1(YDR515W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SLS1(YLR139C)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMK1(YPR054W)|FD-Score:-5.06|P-value:2.07E-7||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SPT21(YMR179W)|FD-Score:5.67|P-value:7.30E-9||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT4(YGR063C)|FD-Score:5.57|P-value:1.30E-8||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUR4(YLR372W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYH1(YPL105C)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TFB5(YDR079C-A)|FD-Score:5.22|P-value:9.00E-8||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TGS1(YPL157W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TIR3(YIL011W)|FD-Score:-4.96|P-value:3.44E-7||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TLG2(YOL018C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPK1(YJL164C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:9.86|P-value:3.13E-23||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.85|P-value:3.62E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.53|P-value:3.33E-11||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TYW1(YPL207W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:VMA4(YOR332W)|FD-Score:4.48|P-value:3.80E-6||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS52(YDR484W)|FD-Score:6.25|P-value:2.10E-10||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL008W-A(YBL008W-A_p)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL029C-A(YBL029C-A_p)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBR071W(YBR071W)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YDR008C(YDR008C_d)|FD-Score:6.67|P-value:1.28E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR282C(YDR282C_p)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YDR340W(YDR340W_d)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Putative protein of unknown function Gene:YDR455C(YDR455C_d)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGL015C(YGL015C_p)|FD-Score:4.62|P-value:1.89E-6||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGL041C(YGL041C_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL088W(YGL088W_d)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR125W(YGR125W_p)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHI9(YHR029C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHR007C-A(YHR007C-A_p)|FD-Score:-3.95|P-value:3.92E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR177W(YHR177W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIL163C(YIL163C_p)|FD-Score:-5.19|P-value:1.02E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YIR007W(YIR007W_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YLR042C(YLR042C_p)|FD-Score:-4.06|P-value:2.50E-5||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR269C(YLR269C_d)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.86|P-value:5.89E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL089C(YNL089C_d)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNL184C(YNL184C_p)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL277W-A(YNL277W-A_p)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function Gene:YOR022C(YOR022C_p)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPK1(YKL126W)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPK3(YBR028C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL035C(YPL035C_d)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:ZRT1(YGL255W)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL201C8.221.03E-163.75MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YDL143W4.473.94E-60.40CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR430W4.072.32E-50.13SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YKL165C3.944.05E-50.05MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YGR094W3.894.97E-50.41VAS1Mitochondrial and cytoplasmic valyl-tRNA synthetase
YDR394W3.482.51E-40.06RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YDR168W3.423.14E-40.19CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YDL195W3.236.12E-40.10SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPL211W3.138.60E-40.04NIP7Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
YBR136W3.109.69E-40.01MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YDR187C_d3.099.94E-40.05YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YJL195C_d3.050.001160.05YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YDR045C3.000.001360.03RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YOR157C2.970.001490.00PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YPR187W2.970.001500.12RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W12.104.70E-34DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YER090W9.863.13E-23TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YKR019C9.152.83E-20IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YKL211C6.853.62E-12TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR008C_d6.671.28E-11YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR354W6.533.33E-11TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR484W6.252.10E-10VPS52Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin
YMR179W5.677.30E-9SPT21Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress
YDR195W5.571.26E-8REF2RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes
YGR063C5.571.30E-8SPT4Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
YNL133C5.511.82E-8FYV6Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
YBL061C5.364.18E-8SKT5Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
YDR079C-A5.229.00E-8TFB5Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair
YBL008W-A_p5.111.64E-7YBL008W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YDR108W5.052.18E-7TRS85Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role

GO enrichment analysis for SGTC_2577
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2633.98E-94SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0470588
0.2549.71E-88SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0645161
0.2539.47E-87SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0952381
0.2512.33E-85SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.104478
0.2501.19E-84SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0909091
0.2432.01E-80SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.190476
0.2387.53E-77SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.0793651
0.2371.89E-76SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.166667
0.2252.79E-68SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0943396
0.2182.11E-64SGTC_2231micatex 6.9 μMMiscellaneous207370.122449
0.2174.53E-64SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.160714
0.2176.60E-64SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.047619
0.2155.60E-63SGTC_1090132-0036 33.7 μMChemDiv (Drug-like library)672740.285714
0.2101.01E-59SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0666667
0.2101.28E-59SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.0447761

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_25792',5'-dihydroxy-4-methoxychalcone13.23 μM0.85355888Microsource (Natural product library)270.279963.20124
SGTC_2565isoliquiritigenin100 μM0.756757638278Microsource (Natural product library)256.253382.97534
SGTC_11730443-0020122 μM0.7073175331295ChemDiv (Drug-like library)254.280563.44313TSC3-RPN4
SGTC_2610ononetin44.17 μM0.555556259632Microsource (Natural product library)258.269262.76824
SGTC_15852',4'-dihydroxychalcone10.4 μM0.5476195357218TimTec (Pure natural product library)240.253983.21823
SGTC_25732',4'-dihydroxychalcone15.04 μM0.5476195357218TimTec (Pure natural product library)240.253983.21823
SGTC_25742',4'-dihydroxychalcone14.64 μM0.5476195357218TimTec (Pure natural product library)240.253983.21823
SGTC_26602',4'-dihydroxychalcone17.14 μM0.5476195357218TimTec (Pure natural product library)240.253983.21823
SGTC_26682',4'-dihydroxychalcone15.08 μM0.5476195357218TimTec (Pure natural product library)240.253983.21823
SGTC_2521angolensin13.4 μM0.543478821428Microsource (Natural product library)272.295843.23124
SGTC_19284'-methoxychalcone40.3 μM0.5641818TimTec (Natural product derivative library)238.281163.68502
SGTC_24984'-Methoxychalcone54.63 μM0.5641818Microsource (Natural product library)238.281163.68502RPP1 & pyrimidine depletion
SGTC_2493resveratrol26.09 μM0.4878056255462Microsource (Natural product library)242.269863.31523
SGTC_1925st05777010.1 μM0.485989122TimTec (Natural product derivative library)284.306543.68724TSC3-RPN4