usnic acid

2,6-diacetyl-7,9-dihydroxy-8,9b-dimethyldibenzofuran-1,3-dione

An anti-infective, antifungal and antitrichomonal agent.

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PubChem MeSH terms: Anti-Infective Agents;Antifungal Agents;Antitrichomonal Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_259
Screen concentration 108.9 μM
Source Miscellaneous
PubChem CID 5646
SMILES CC1=C(C(=C2C(=C1O)C3(C(=CC(=O)C(C3=O)C(=O)C)O2)C)C(=O)C)O
Standardized SMILES CC(=O)C1C(=O)C=C2Oc3c(C(=O)C)c(O)c(C)c(O)c3C2(C)C1=O
Molecular weight 344.3154
ALogP 1.08
H-bond donor count 2
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.13
% growth inhibition (Hom. pool) -1.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5646
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:-3.29|P-value:5.06E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC31(YOR257W)|FD-Score:-3.45|P-value:2.83E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:ERG12(YMR208W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.05||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.04||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:LAS1(YKR063C)|FD-Score:5.27|P-value:6.98E-8|Clearance:0.93||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:PFS2(YNL317W)|FD-Score:3.11|P-value:9.46E-4|Clearance:0.02||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PGS1(YCL004W)|FD-Score:3.78|P-value:7.77E-5|Clearance:0.04||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP4(YPR178W)|FD-Score:7.79|P-value:3.23E-15|Clearance:2.09||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PTI1(YGR156W)|FD-Score:5.3|P-value:5.75E-8|Clearance:0.04||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RNA1(YMR235C)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.13||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RNA14(YMR061W)|FD-Score:4.02|P-value:2.87E-5|Clearance:0.24||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RNA15(YGL044C)|FD-Score:3.75|P-value:9.00E-5|Clearance:0.02||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPS15(YOL040C)|FD-Score:-3.1|P-value:9.70E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SPC42(YKL042W)|FD-Score:3.72|P-value:9.94E-5|Clearance:0.09||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPT14(YPL175W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.17||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SUA5(YGL169W)|FD-Score:4.34|P-value:7.25E-6|Clearance:0.31||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TOM40(YMR203W)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTR5(YEL035C_p)|FD-Score:5.7|P-value:5.85E-9|Clearance:0.4||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YJL032W(YJL032W_d)|FD-Score:3.42|P-value:3.13E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:YLR230W(YLR230W_d)|FD-Score:-3.12|P-value:9.15E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:ARP7(YPR034W)|FD-Score:-3.29|P-value:5.06E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC31(YOR257W)|FD-Score:-3.45|P-value:2.83E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:ERG12(YMR208W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.05||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.04||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:LAS1(YKR063C)|FD-Score:5.27|P-value:6.98E-8|Clearance:0.93||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:PFS2(YNL317W)|FD-Score:3.11|P-value:9.46E-4|Clearance:0.02||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PGS1(YCL004W)|FD-Score:3.78|P-value:7.77E-5|Clearance:0.04||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP4(YPR178W)|FD-Score:7.79|P-value:3.23E-15|Clearance:2.09||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PTI1(YGR156W)|FD-Score:5.3|P-value:5.75E-8|Clearance:0.04||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RNA1(YMR235C)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.13||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RNA14(YMR061W)|FD-Score:4.02|P-value:2.87E-5|Clearance:0.24||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RNA15(YGL044C)|FD-Score:3.75|P-value:9.00E-5|Clearance:0.02||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPS15(YOL040C)|FD-Score:-3.1|P-value:9.70E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SPC42(YKL042W)|FD-Score:3.72|P-value:9.94E-5|Clearance:0.09||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPT14(YPL175W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.17||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SUA5(YGL169W)|FD-Score:4.34|P-value:7.25E-6|Clearance:0.31||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TOM40(YMR203W)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTR5(YEL035C_p)|FD-Score:5.7|P-value:5.85E-9|Clearance:0.4||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YJL032W(YJL032W_d)|FD-Score:3.42|P-value:3.13E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:YLR230W(YLR230W_d)|FD-Score:-3.12|P-value:9.15E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5646
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIF1(YNR074C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:ARG81(YML099C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:AVT1(YJR001W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AVT5(YBL089W)|FD-Score:-3.8|P-value:7.25E-5||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:CBS1(YDL069C)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CDH1(YGL003C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CHS5(YLR330W)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CNL1(YDR357C_p)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:COG6(YNL041C)|FD-Score:6.64|P-value:1.56E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS111(YBR203W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:COX12(YLR038C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CTF3(YLR381W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:CTK1(YKL139W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CTR1(YPR124W)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUS2(YNL286W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:ERV46(YAL042W)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:ETR1(YBR026C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FMP37(YGL080W)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FRK1(YPL141C)|FD-Score:7.69|P-value:7.48E-15||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GAL83(YER027C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GRX4(YER174C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HEH2(YDR458C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HSP26(YBR072W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:IES1(YFL013C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:ILV6(YCL009C)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IST1(YNL265C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:MET13(YGL125W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MRPL44(YMR225C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NMA111(YNL123W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NNF2(YGR089W)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUP120(YKL057C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OPI3(YJR073C)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PUS1(YPL212C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RCF1(YML030W)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RFS1(YBR052C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RHR2(YIL053W)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS24A(YER074W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:SER3(YER081W)|FD-Score:5.01|P-value:2.77E-7||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SIC1(YLR079W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKN7(YHR206W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SNX41(YDR425W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p Gene:SOP4(YJL192C)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SSE1(YPL106C)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:TRX1(YLR043C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBX6(YJL048C)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VPS21(YOR089C)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBP2(YGL060W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR134W(YBR134W_d)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCR007C(YCR007C_p)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL026W(YDL026W_d)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHC3(YJL059W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIL032C(YIL032C_d)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR152C(YLR152C_p)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YME2(YMR302C)|FD-Score:7.26|P-value:1.96E-13||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML020W(YML020W_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function Gene:YMR158C-A(YMR158C-A_p)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:7.12|P-value:5.51E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL197C(YPL197C_d)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:ACB1(YGR037C)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIF1(YNR074C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:ARG81(YML099C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:AVT1(YJR001W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AVT5(YBL089W)|FD-Score:-3.8|P-value:7.25E-5||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:CBS1(YDL069C)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CDH1(YGL003C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CHS5(YLR330W)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CNL1(YDR357C_p)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:COG6(YNL041C)|FD-Score:6.64|P-value:1.56E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS111(YBR203W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:COX12(YLR038C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CTF3(YLR381W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:CTK1(YKL139W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CTR1(YPR124W)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUS2(YNL286W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:ERV46(YAL042W)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:ETR1(YBR026C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FMP37(YGL080W)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FRK1(YPL141C)|FD-Score:7.69|P-value:7.48E-15||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GAL83(YER027C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GRX4(YER174C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HEH2(YDR458C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HSP26(YBR072W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:IES1(YFL013C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:ILV6(YCL009C)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IST1(YNL265C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:MET13(YGL125W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MRPL44(YMR225C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NMA111(YNL123W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NNF2(YGR089W)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUP120(YKL057C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OPI3(YJR073C)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PUS1(YPL212C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RCF1(YML030W)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RFS1(YBR052C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RHR2(YIL053W)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS24A(YER074W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:SER3(YER081W)|FD-Score:5.01|P-value:2.77E-7||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SIC1(YLR079W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKN7(YHR206W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SNX41(YDR425W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p Gene:SOP4(YJL192C)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SSE1(YPL106C)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:TRX1(YLR043C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBX6(YJL048C)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VPS21(YOR089C)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBP2(YGL060W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR134W(YBR134W_d)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCR007C(YCR007C_p)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL026W(YDL026W_d)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHC3(YJL059W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIL032C(YIL032C_d)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR152C(YLR152C_p)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YME2(YMR302C)|FD-Score:7.26|P-value:1.96E-13||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML020W(YML020W_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function Gene:YMR158C-A(YMR158C-A_p)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:7.12|P-value:5.51E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL197C(YPL197C_d)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR178W7.793.23E-152.09PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YEL035C_p5.705.85E-90.40UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YGR156W5.305.75E-80.04PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YKR063C5.276.98E-80.93LAS1Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
YGL169W4.347.25E-60.31SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YMR061W4.022.87E-50.24RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YCL004W3.787.77E-50.04PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YGL044C3.759.00E-50.02RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YKL042W3.729.94E-50.09SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YPL175W3.631.42E-40.17SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YJL085W3.462.67E-40.04EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YJL032W_d3.423.13E-40.08YJL032W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
YMR208W3.344.13E-40.05ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YMR203W3.304.85E-40.06TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YMR235C3.245.97E-40.13RNA1GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL141C7.697.48E-15FRK1Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
YMR302C7.261.96E-13YME2Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
YNL170W_d7.125.51E-13YNL170W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL041C6.641.56E-11COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YER081W5.012.77E-7SER33-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication
YMR158C-A_p4.681.43E-6YMR158C-A_pPutative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene
YER027C4.602.13E-6GAL83One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain
YPL106C4.542.76E-6SSE1ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication
YBR203W4.542.83E-6COS111Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YML030W4.513.24E-6RCF1Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast
YGL080W4.464.16E-6FMP37Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia
YGL003C4.211.28E-5CDH1Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p
YNL286W4.191.38E-5CUS2Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs)
YNR074C4.131.83E-5AIF1Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase
YJL048C4.092.19E-5UBX6UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication

GO enrichment analysis for SGTC_259
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1262.03E-22SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0581395DNA intercalators
0.1063.38E-16SGTC_512prima-1 270.0 μMICCB bioactive library3229680.114754DNA intercalators
0.1063.79E-16SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.09375DNA intercalators
0.1054.96E-16SGTC_1250133-0007 11.8 μMChemDiv (Drug-like library)265410.0517241DNA intercalators
0.1057.76E-16SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.166667DNA intercalators
0.0961.93E-13SGTC_32839109332 35.8 μMChembridge (Drug-like library)170272810.0823529DNA intercalators
0.0881.30E-11SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.0606061DNA intercalators
0.0862.81E-11SGTC_1732st034308 51.8 μMTimTec (Natural product derivative library)8301190.136364
0.0841.00E-10SGTC_7420kpi-0133 140.0 μMChemDiv (Drug-like library)27288520.111111ERG2
0.0781.58E-9SGTC_1260417-1624 672.5 μMChemDiv (Drug-like library)419120.147059DNA intercalators
0.0773.30E-9SGTC_12961068-0102 11.9 μMChemDiv (Drug-like library)7670530.0666667
0.0757.78E-9SGTC_14813346-2004 36.0 μMChemDiv (Drug-like library)27705220.0777778
0.0731.94E-8SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.0740741DNA intercalators
0.0706.69E-8SGTC_1453737-0113 38.4 μMChemDiv (Drug-like library)68493510.0769231DNA intercalators
0.0707.98E-8SGTC_170960-0276 49.8 μMChemDiv (Drug-like library)68211140.0729167

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2690usnic acid100 μM15646Miscellaneous344.315441.08427
SGTC_2529gangaleoidin93.61 μM0.2133334565183Microsource (Natural product library)413.205564.8917
SGTC_21235259487200 μM0.196972173474Chembridge (Fragment library)223.268281.09114
SGTC_1570verbenone133 μM0.19642929025TimTec (Pure natural product library)150.217562.08201
SGTC_2561acetosyringone100 μM0.19642917198Microsource (Natural product library)196.199881.29514
SGTC_2496diffractaic acid79.83 μM0.18840694870Microsource (Natural product library)374.384484.56127
SGTC_13973532-019020.1 μM0.18755322280ChemDiv (Drug-like library)230.259163.57713
SGTC_25534-methylesculetin85.09 μM0.1864415319502Microsource (Natural product library)192.168121.86224
SGTC_1569carminic acid40.6 μM0.18518514749TimTec (Pure natural product library)492.3864-0.436913
SGTC_2490carminic acid95.39 μM0.18518514749TimTec (Pure natural product library)492.3864-0.436913
SGTC_1959st07709155.8 μM0.173913821356TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion