skatole

3-methyl-1H-indole

A reactive methylindole and nucleophilic thiol agent. Glutathione protects alkylation of proteins by a skatole metabolite.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2591
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 6736
SMILES CC1=CNC2=CC=CC=C12
Standardized SMILES Cc1c[nH]c2ccccc12
Molecular weight 131.1745
ALogP 2.61
H-bond donor count 1
H-bond acceptor count 0
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.68
% growth inhibition (Hom. pool) 4.72


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6736
Download HIP data (tab-delimited text)  (excel)
Gene:CAB1(YDR531W)|FD-Score:-3.87|P-value:5.54E-5|Clearance:0||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CDC8(YJR057W)|FD-Score:3.78|P-value:7.85E-5|Clearance:0.13||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CFT1(YDR301W)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.01||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:IPP1(YBR011C)|FD-Score:3.11|P-value:9.30E-4|Clearance:0.01||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:LSM4(YER112W)|FD-Score:-3.36|P-value:3.89E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MEC1(YBR136W)|FD-Score:4.5|P-value:3.41E-6|Clearance:0.4||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NIC96(YFR002W)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.18||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP15(YNL110C)|FD-Score:3.33|P-value:4.30E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NUP57(YGR119C)|FD-Score:6.74|P-value:7.69E-12|Clearance:2.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:NUP85(YJR042W)|FD-Score:3.32|P-value:4.46E-4|Clearance:0.02||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PDC2(YDR081C)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.04||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PDS5(YMR076C)|FD-Score:4.58|P-value:2.36E-6|Clearance:0.08||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRP2(YNR011C)|FD-Score:-8.16|P-value:1.74E-16|Clearance:0||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:PSE1(YMR308C)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:RRP42(YDL111C)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.2||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SLD2(YKL108W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.04||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR341C(YDR341C)|FD-Score:4.1|P-value:2.10E-5|Clearance:0.32||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YFH1(YDL120W)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.02||SGD DESC:Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia Gene:YGR265W(YGR265W_d)|FD-Score:3.1|P-value:9.61E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:CAB1(YDR531W)|FD-Score:-3.87|P-value:5.54E-5|Clearance:0||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CDC8(YJR057W)|FD-Score:3.78|P-value:7.85E-5|Clearance:0.13||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CFT1(YDR301W)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.01||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:IPP1(YBR011C)|FD-Score:3.11|P-value:9.30E-4|Clearance:0.01||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:LSM4(YER112W)|FD-Score:-3.36|P-value:3.89E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MEC1(YBR136W)|FD-Score:4.5|P-value:3.41E-6|Clearance:0.4||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NIC96(YFR002W)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.18||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP15(YNL110C)|FD-Score:3.33|P-value:4.30E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NUP57(YGR119C)|FD-Score:6.74|P-value:7.69E-12|Clearance:2.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:NUP85(YJR042W)|FD-Score:3.32|P-value:4.46E-4|Clearance:0.02||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PDC2(YDR081C)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.04||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PDS5(YMR076C)|FD-Score:4.58|P-value:2.36E-6|Clearance:0.08||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRP2(YNR011C)|FD-Score:-8.16|P-value:1.74E-16|Clearance:0||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:PSE1(YMR308C)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:RRP42(YDL111C)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.2||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SLD2(YKL108W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.04||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR341C(YDR341C)|FD-Score:4.1|P-value:2.10E-5|Clearance:0.32||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YFH1(YDL120W)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.02||SGD DESC:Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia Gene:YGR265W(YGR265W_d)|FD-Score:3.1|P-value:9.61E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6736
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:4.76|P-value:9.88E-7||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AFG3(YER017C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ALG12(YNR030W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:APS2(YJR058C)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:ARA2(YMR041C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ASN1(YPR145W)|FD-Score:5.24|P-value:7.98E-8||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:ATP2(YJR121W)|FD-Score:9.05|P-value:7.13E-20||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BDF2(YDL070W)|FD-Score:-3.83|P-value:6.53E-5||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BMH1(YER177W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BUL2(YML111W)|FD-Score:-4.06|P-value:2.42E-5||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CIT3(YPR001W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CLB4(YLR210W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:CLU1(YMR012W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:CYC1(YJR048W)|FD-Score:-5.03|P-value:2.45E-7||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:DAL3(YIR032C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DCN1(YLR128W)|FD-Score:4.28|P-value:9.17E-6||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:ECM31(YBR176W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ECM32(YER176W)|FD-Score:-4.25|P-value:1.09E-5||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ERP3(YDL018C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FCY21(YER060W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GEA1(YJR031C)|FD-Score:-4.65|P-value:1.67E-6||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GIM5(YML094W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GMC2(YLR445W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GNP1(YDR508C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HUA1(YGR268C)|FD-Score:-4.42|P-value:4.94E-6||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IDH2(YOR136W)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IES3(YLR052W)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC14(YOR135C_d)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:JHD2(YJR119C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:KHA1(YJL094C)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:KIN82(YCR091W)|FD-Score:6.01|P-value:9.22E-10||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:KNH1(YDL049C)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LIA1(YJR070C)|FD-Score:5.66|P-value:7.73E-9||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MCH5(YOR306C)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MCM21(YDR318W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MRK1(YDL079C)|FD-Score:-5.64|P-value:8.57E-9||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL23(YOR150W)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB3(YNL293W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSC3(YLR219W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:MSR1(YHR091C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:NRP1(YDL167C)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NUP2(YLR335W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:NVJ1(YHR195W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OAR1(YKL055C)|FD-Score:5.53|P-value:1.60E-8||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PHM8(YER037W)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PSR1(YLL010C)|FD-Score:-5.04|P-value:2.38E-7||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RAD6(YGL058W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RCF1(YML030W)|FD-Score:7.13|P-value:4.91E-13||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RIB4(YOL143C)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RIC1(YLR039C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RRI2(YOL117W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SAS4(YDR181C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SDS3(YIL084C)|FD-Score:6.91|P-value:2.43E-12||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SER33(YIL074C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SGF73(YGL066W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SNT309(YPR101W)|FD-Score:4.89|P-value:5.08E-7||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPL2(YHR136C)|FD-Score:5.21|P-value:9.37E-8||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SPO11(YHL022C)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPO13(YHR014W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II Gene:SPS100(YHR139C)|FD-Score:8.41|P-value:1.97E-17||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SPT2(YER161C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSP1(YHR184W)|FD-Score:4.89|P-value:5.11E-7||SGD DESC:Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis Gene:SSP2(YOR242C)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:STE24(YJR117W)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUC2(YIL162W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SWE1(YJL187C)|FD-Score:-4.65|P-value:1.67E-6||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:THI72(YOR192C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TOS2(YGR221C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TRE1(YPL176C)|FD-Score:-4.83|P-value:6.74E-7||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UAF30(YOR295W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:VPS38(YLR360W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:YCL023C(YCL023C_d)|FD-Score:4.39|P-value:5.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCL049C(YCL049C_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDL009C(YDL009C_p)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YEL020C(YEL020C_p)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YGL235W(YGL235W_p)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL260W(YGL260W_p)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR051C(YGR051C_d)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YIR043C(YIR043C)|FD-Score:4.81|P-value:7.61E-7||SGD DESC:Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL070C(YJL070C_p)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YKR070W(YKR070W_p)|FD-Score:4.78|P-value:8.55E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR241W(YLR241W_p)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YML009C-A(YML009C-A_d)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML108W(YML108W_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YMR105W-A(YMR105W-A_p)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Putative protein of unknown function Gene:YND1(YER005W)|FD-Score:-4.17|P-value:1.55E-5||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL050C(YNL050C_p)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YPL216W(YPL216W_p)|FD-Score:-4.89|P-value:5.01E-7||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPR022C(YPR022C_p)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:ADE1(YAR015W)|FD-Score:4.76|P-value:9.88E-7||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AFG3(YER017C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ALG12(YNR030W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:APS2(YJR058C)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:ARA2(YMR041C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ASN1(YPR145W)|FD-Score:5.24|P-value:7.98E-8||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:ATP2(YJR121W)|FD-Score:9.05|P-value:7.13E-20||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BDF2(YDL070W)|FD-Score:-3.83|P-value:6.53E-5||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BMH1(YER177W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BUL2(YML111W)|FD-Score:-4.06|P-value:2.42E-5||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CIT3(YPR001W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CLB4(YLR210W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:CLU1(YMR012W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:CYC1(YJR048W)|FD-Score:-5.03|P-value:2.45E-7||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:DAL3(YIR032C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DCN1(YLR128W)|FD-Score:4.28|P-value:9.17E-6||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:ECM31(YBR176W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ECM32(YER176W)|FD-Score:-4.25|P-value:1.09E-5||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ERP3(YDL018C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FCY21(YER060W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GEA1(YJR031C)|FD-Score:-4.65|P-value:1.67E-6||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GIM5(YML094W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GMC2(YLR445W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GNP1(YDR508C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HUA1(YGR268C)|FD-Score:-4.42|P-value:4.94E-6||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IDH2(YOR136W)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IES3(YLR052W)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC14(YOR135C_d)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:JHD2(YJR119C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:KHA1(YJL094C)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:KIN82(YCR091W)|FD-Score:6.01|P-value:9.22E-10||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:KNH1(YDL049C)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LIA1(YJR070C)|FD-Score:5.66|P-value:7.73E-9||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MCH5(YOR306C)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MCM21(YDR318W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MRK1(YDL079C)|FD-Score:-5.64|P-value:8.57E-9||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL23(YOR150W)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB3(YNL293W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSC3(YLR219W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:MSR1(YHR091C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:NRP1(YDL167C)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NUP2(YLR335W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:NVJ1(YHR195W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OAR1(YKL055C)|FD-Score:5.53|P-value:1.60E-8||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PHM8(YER037W)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PSR1(YLL010C)|FD-Score:-5.04|P-value:2.38E-7||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RAD6(YGL058W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RCF1(YML030W)|FD-Score:7.13|P-value:4.91E-13||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RIB4(YOL143C)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RIC1(YLR039C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RRI2(YOL117W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SAS4(YDR181C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SDS3(YIL084C)|FD-Score:6.91|P-value:2.43E-12||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SER33(YIL074C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SGF73(YGL066W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SNT309(YPR101W)|FD-Score:4.89|P-value:5.08E-7||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPL2(YHR136C)|FD-Score:5.21|P-value:9.37E-8||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SPO11(YHL022C)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPO13(YHR014W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II Gene:SPS100(YHR139C)|FD-Score:8.41|P-value:1.97E-17||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SPT2(YER161C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSP1(YHR184W)|FD-Score:4.89|P-value:5.11E-7||SGD DESC:Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis Gene:SSP2(YOR242C)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:STE24(YJR117W)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUC2(YIL162W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SWE1(YJL187C)|FD-Score:-4.65|P-value:1.67E-6||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:THI72(YOR192C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TOS2(YGR221C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TRE1(YPL176C)|FD-Score:-4.83|P-value:6.74E-7||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UAF30(YOR295W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:VPS38(YLR360W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:YCL023C(YCL023C_d)|FD-Score:4.39|P-value:5.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCL049C(YCL049C_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDL009C(YDL009C_p)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YEL020C(YEL020C_p)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YGL235W(YGL235W_p)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL260W(YGL260W_p)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR051C(YGR051C_d)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YIR043C(YIR043C)|FD-Score:4.81|P-value:7.61E-7||SGD DESC:Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL070C(YJL070C_p)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YKR070W(YKR070W_p)|FD-Score:4.78|P-value:8.55E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR241W(YLR241W_p)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YML009C-A(YML009C-A_d)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML108W(YML108W_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YMR105W-A(YMR105W-A_p)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Putative protein of unknown function Gene:YND1(YER005W)|FD-Score:-4.17|P-value:1.55E-5||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL050C(YNL050C_p)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YPL216W(YPL216W_p)|FD-Score:-4.89|P-value:5.01E-7||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPR022C(YPR022C_p)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR119C6.747.69E-122.17NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YMR076C4.582.36E-60.08PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YBR136W4.503.41E-60.40MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YDR341C4.102.10E-50.32YDR341CArginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YJR057W3.787.85E-50.13CDC8Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
YDR081C3.651.31E-40.04PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YDR280W3.611.51E-40.20RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YDL111C3.423.16E-40.02RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YKL108W3.403.43E-40.04SLD2Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
YDL120W3.353.97E-40.02YFH1Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
YNL110C3.334.30E-40.01NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YJR042W3.324.46E-40.02NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YDR301W3.304.86E-40.01CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YFR002W3.294.95E-40.18NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YBR011C3.119.30E-40.01IPP1Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR121W9.057.13E-20ATP2Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YHR139C8.411.97E-17SPS100Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin
YML030W7.134.91E-13RCF1Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast
YIL084C6.912.43E-12SDS3Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes
YCR091W6.019.22E-10KIN82Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p
YJR070C5.667.73E-9LIA1Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
YKL055C5.531.60E-8OAR1Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p
YPR145W5.247.98E-8ASN1Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
YHR136C5.219.37E-8SPL2Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm
YMR105W-A_p4.992.96E-7YMR105W-A_pPutative protein of unknown function
YPR101W4.895.08E-7SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
YHR184W4.895.11E-7SSP1Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis
YJR058C4.836.77E-7APS2Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
YIR043C4.817.61E-7YIR043CPossible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
YKR070W_p4.788.55E-7YKR070W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_2591
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0663.54E-7SGTC_2234tolnaftate 200.0 μMMiscellaneous55100.14
0.0655.47E-7SGTC_23709071495 200.0 μMChembridge (Fragment library)217911030.1mitochondrial processes
0.0604.70E-6SGTC_10775-fluorocytosine 377.0 nMNIH Clinical Collection33660.05exosome
0.0561.95E-5SGTC_32219128209 49.5 μMChembridge (Drug-like library)164607230.103448
0.0552.38E-5SGTC_2630antimycin a 100.0 μMMicrosource (Natural product library)57021990.060241mitochondrial processes
0.0534.29E-5SGTC_21765809774 34.0 μMChembridge (Fragment library)28705390.142857mitochondrial processes
0.0519.20E-5SGTC_31169124943 49.5 μMChembridge (Drug-like library)419051950.111111
0.0511.01E-4SGTC_1823448-4371 550.5 μMChemDiv (Drug-like library)34914030.101695
0.0501.11E-4SGTC_1906st060206 94.7 μMTimTec (Natural product derivative library)51334680.104167
0.0501.14E-4SGTC_1693xanthone 101.9 μMTimTec (Natural product derivative library)70200.151515DNA damage response
0.0491.69E-4SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.05exosome
0.0491.75E-4SGTC_20685226020 147.0 μMChembridge (Fragment library)18105090.15
0.0482.24E-4SGTC_8800833-0487 87.2 μMChemDiv (Drug-like library)760750.125mitochondrial processes
0.0482.51E-4SGTC_1235benzylparaben 21.5 μMChemDiv (Drug-like library)71800.116279
0.0472.76E-4SGTC_1932st074806 40.9 μMTimTec (Natural product derivative library)172512350.0921053

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_452diindolylmethane8 μM0.5185193071ICCB bioactive library246.306464.39120RNA processing & uracil transport
SGTC_2588indole-3-carbinol100 μM0.4827593712Microsource (Natural product library)147.173861.51821
SGTC_2017513292760.65 μM0.4285718367Chembridge (Fragment library)188.268842.45121
SGTC_20515263410176 μM0.405405227584Chembridge (Fragment library)229.320762.15412
SGTC_1825st05214362.6 μM0.4674173TimTec (Natural product derivative library)319.44334.22412
SGTC_1774st04837148.47 μM0.3902444242727TimTec (Natural product derivative library)318.415483.47412RNA processing & uracil transport
SGTC_210849-011322.99 μM0.3888891560156ChemDiv (Drug-like library)188.182722.10312
SGTC_690096-0274206.68 μM0.3756741392ChemDiv (Drug-like library)248.322343.71812mitochondrial processes
SGTC_1754st04206516.14 μM0.351126236TimTec (Natural product derivative library)355.47544.64612NEO1-PIK1
SGTC_9453381-076811.3 μM0.351126236ChemDiv (Drug-like library)355.47544.64612endomembrane recycling