a-mangostin

1,3,6,7-tetramethoxy-2,8-bis(3-methylbut-2-enyl)xanthen-9-one

Xanthone derivative isolated from mangosteen (Garcinia mangostana). Antioxidant and anti-inflammatory. α-mangostin has been shown to induce apoptosis, reduce cell proliferation and inhibit tumorigenesis.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2594
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 45934201
SMILES CC(=CCC1=C(C=C2C(=C1OC)C(=O)C3=C(C(=C(C=C3O2)OC)OC)CC=C(C)C)OC)C
Standardized SMILES COc1cc2Oc3cc(OC)c(OC)c(CC=C(C)C)c3C(=O)c2c(OC)c1CC=C(C)C
Molecular weight 452.5394
ALogP 6.61
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.25
% growth inhibition (Hom. pool) 9.88


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 45934201
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.06||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CDC37(YDR168W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.11||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:CDC9(YDL164C)|FD-Score:5.56|P-value:1.33E-8|Clearance:0.9||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:CEP3(YMR168C)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.08||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CWC2(YDL209C)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.01||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DBP9(YLR276C)|FD-Score:3.22|P-value:6.40E-4|Clearance:0.15||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:GPI8(YDR331W)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.02||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:KOG1(YHR186C)|FD-Score:3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:NIP7(YPL211W)|FD-Score:-3.09|P-value:9.90E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:PKC1(YBL105C)|FD-Score:-3.33|P-value:4.31E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RFC5(YBR087W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.03||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPB8(YOR224C)|FD-Score:-3.1|P-value:9.82E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:SAS10(YDL153C)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.01||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SEC2(YNL272C)|FD-Score:-3.79|P-value:7.53E-5|Clearance:0||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:SEC6(YIL068C)|FD-Score:5.08|P-value:1.87E-7|Clearance:0.9||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SEN34(YAR008W)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.01||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SOG2(YOR353C)|FD-Score:-3.3|P-value:4.88E-4|Clearance:0||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:SRP14(YDL092W)|FD-Score:3.91|P-value:4.62E-5|Clearance:0.21||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TAF5(YBR198C)|FD-Score:5.22|P-value:9.02E-8|Clearance:0.9||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TSR4(YOL022C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.02||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:TUB1(YML085C)|FD-Score:3.95|P-value:3.96E-5|Clearance:0.04||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YGR114C(YGR114C_d)|FD-Score:4.1|P-value:2.04E-5|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YPR142C(YPR142C_d)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:ACT1(YFL039C)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.06||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CDC37(YDR168W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.11||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:CDC9(YDL164C)|FD-Score:5.56|P-value:1.33E-8|Clearance:0.9||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:CEP3(YMR168C)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.08||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CWC2(YDL209C)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.01||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DBP9(YLR276C)|FD-Score:3.22|P-value:6.40E-4|Clearance:0.15||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:GPI8(YDR331W)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.02||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:KOG1(YHR186C)|FD-Score:3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:NIP7(YPL211W)|FD-Score:-3.09|P-value:9.90E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:PKC1(YBL105C)|FD-Score:-3.33|P-value:4.31E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RFC5(YBR087W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.03||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPB8(YOR224C)|FD-Score:-3.1|P-value:9.82E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:SAS10(YDL153C)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.01||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SEC2(YNL272C)|FD-Score:-3.79|P-value:7.53E-5|Clearance:0||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:SEC6(YIL068C)|FD-Score:5.08|P-value:1.87E-7|Clearance:0.9||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SEN34(YAR008W)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.01||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SOG2(YOR353C)|FD-Score:-3.3|P-value:4.88E-4|Clearance:0||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:SRP14(YDL092W)|FD-Score:3.91|P-value:4.62E-5|Clearance:0.21||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TAF5(YBR198C)|FD-Score:5.22|P-value:9.02E-8|Clearance:0.9||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TSR4(YOL022C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.02||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:TUB1(YML085C)|FD-Score:3.95|P-value:3.96E-5|Clearance:0.04||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YGR114C(YGR114C_d)|FD-Score:4.1|P-value:2.04E-5|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YPR142C(YPR142C_d)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 45934201
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ADE5,7(YGL234W)|FD-Score:8.66|P-value:2.44E-18||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:ADH2(YMR303C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:ARC18(YLR370C)|FD-Score:4.83|P-value:6.68E-7||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG7(YMR062C)|FD-Score:-4.55|P-value:2.68E-6||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ARO2(YGL148W)|FD-Score:4.48|P-value:3.80E-6||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BTS1(YPL069C)|FD-Score:-4.27|P-value:9.59E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCW12(YLR110C)|FD-Score:7|P-value:1.29E-12||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CUE3(YGL110C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination Gene:DAL81(YIR023W)|FD-Score:6.52|P-value:3.53E-11||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DET1(YDR051C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:DIG2(YDR480W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:EGD2(YHR193C)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:EPT1(YHR123W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:ETT1(YOR051C)|FD-Score:5.42|P-value:2.96E-8||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FMP27(YLR454W_p)|FD-Score:-5.73|P-value:5.06E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPR1(YNL135C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GAL11(YOL051W)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GDS1(YOR355W)|FD-Score:4.44|P-value:4.48E-6||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GID7(YCL039W)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GRX7(YBR014C)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GSM1(YJL103C)|FD-Score:-3.89|P-value:5.09E-5||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:HBS1(YKR084C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HTA1(YDR225W)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:HXT10(YFL011W)|FD-Score:5.78|P-value:3.66E-9||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:ILM1(YJR118C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IMO32(YGR031W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:JJJ3(YJR097W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KIN2(YLR096W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:MDM30(YLR368W)|FD-Score:-3.88|P-value:5.12E-5||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MNN10(YDR245W)|FD-Score:5.75|P-value:4.49E-9||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:NDE1(YMR145C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NOT5(YPR072W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPL3(YDR432W)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NRT1(YOR071C)|FD-Score:7.1|P-value:6.22E-13||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:OPI8(YKR035C_d)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OSH6(YKR003W)|FD-Score:6.25|P-value:2.11E-10||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:OSW1(YOR255W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAI3(YMR174C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PEX15(YOL044W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PNG1(YPL096W)|FD-Score:-4.28|P-value:9.30E-6||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPS1(YBR276C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:PRB1(YEL060C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PTK2(YJR059W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUF4(YGL014W)|FD-Score:-4.33|P-value:7.62E-6||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:PUS2(YGL063W)|FD-Score:-3.75|P-value:8.82E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RFX1(YLR176C)|FD-Score:12.8|P-value:1.55E-37||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RGP1(YDR137W)|FD-Score:-6.32|P-value:1.27E-10||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIF1(YBR275C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL27A(YHR010W)|FD-Score:4.45|P-value:4.34E-6||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:4.32|P-value:7.65E-6||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS12(YOR369C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RPS8A(YBL072C)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RUP1(YOR138C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SAP185(YJL098W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGE1(YPR198W)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHC1(YER096W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SHU1(YHL006C)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIT1(YEL065W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p Gene:SIT4(YDL047W)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLT2(YHR030C)|FD-Score:-4.09|P-value:2.19E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF2(YOR290C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPI1(YER150W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO1(YNL012W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPS100(YHR139C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SPT23(YKL020C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SSN3(YPL042C)|FD-Score:-4.4|P-value:5.39E-6||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STE50(YCL032W)|FD-Score:4.4|P-value:5.40E-6||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUR4(YLR372W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC7(YLR385C)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:SWF1(YDR126W)|FD-Score:-5.76|P-value:4.22E-9||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SYF2(YGR129W)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TEC1(YBR083W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member Gene:TGS1(YPL157W)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOF2(YKR010C)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS65(YGR166W)|FD-Score:-4.94|P-value:3.82E-7||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:VAC8(YEL013W)|FD-Score:7.87|P-value:1.79E-15||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VID28(YIL017C)|FD-Score:-3.86|P-value:5.67E-5||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:WTM1(YOR230W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBL055C(YBL055C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR141C(YBR141C_p)|FD-Score:-5.18|P-value:1.12E-7||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCL046W(YCL046W_d)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCP4(YCR004C)|FD-Score:6.32|P-value:1.32E-10||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YEL1(YBL060W)|FD-Score:5.05|P-value:2.26E-7||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YGL260W(YGL260W_p)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR064W(YGR064W_d)|FD-Score:5.23|P-value:8.58E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR107W(YGR107W_d)|FD-Score:-4.44|P-value:4.50E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHB1(YGR234W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YHC3(YJL059W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YKL027W(YKL027W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL070W(YKL070W_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR202C(YLR202C_d)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR297W(YLR297W_p)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:6.63|P-value:1.71E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR248W(YOR248W_d)|FD-Score:6.13|P-value:4.48E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL205C(YPL205C_d)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPS1(YLR120C)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT6(YLR262C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ABZ2(YMR289W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ADE5,7(YGL234W)|FD-Score:8.66|P-value:2.44E-18||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:ADH2(YMR303C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:ARC18(YLR370C)|FD-Score:4.83|P-value:6.68E-7||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG7(YMR062C)|FD-Score:-4.55|P-value:2.68E-6||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ARO2(YGL148W)|FD-Score:4.48|P-value:3.80E-6||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BTS1(YPL069C)|FD-Score:-4.27|P-value:9.59E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCW12(YLR110C)|FD-Score:7|P-value:1.29E-12||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CUE3(YGL110C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination Gene:DAL81(YIR023W)|FD-Score:6.52|P-value:3.53E-11||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DET1(YDR051C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:DIG2(YDR480W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:EGD2(YHR193C)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:EPT1(YHR123W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:ETT1(YOR051C)|FD-Score:5.42|P-value:2.96E-8||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FMP27(YLR454W_p)|FD-Score:-5.73|P-value:5.06E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPR1(YNL135C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GAL11(YOL051W)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GDS1(YOR355W)|FD-Score:4.44|P-value:4.48E-6||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GID7(YCL039W)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GRX7(YBR014C)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GSM1(YJL103C)|FD-Score:-3.89|P-value:5.09E-5||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:HBS1(YKR084C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HTA1(YDR225W)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:HXT10(YFL011W)|FD-Score:5.78|P-value:3.66E-9||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:ILM1(YJR118C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IMO32(YGR031W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:JJJ3(YJR097W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KIN2(YLR096W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:MDM30(YLR368W)|FD-Score:-3.88|P-value:5.12E-5||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MNN10(YDR245W)|FD-Score:5.75|P-value:4.49E-9||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:NDE1(YMR145C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NOT5(YPR072W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPL3(YDR432W)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NRT1(YOR071C)|FD-Score:7.1|P-value:6.22E-13||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:OPI8(YKR035C_d)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OSH6(YKR003W)|FD-Score:6.25|P-value:2.11E-10||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:OSW1(YOR255W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAI3(YMR174C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PEX15(YOL044W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PNG1(YPL096W)|FD-Score:-4.28|P-value:9.30E-6||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPS1(YBR276C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:PRB1(YEL060C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PTK2(YJR059W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUF4(YGL014W)|FD-Score:-4.33|P-value:7.62E-6||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:PUS2(YGL063W)|FD-Score:-3.75|P-value:8.82E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RFX1(YLR176C)|FD-Score:12.8|P-value:1.55E-37||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RGP1(YDR137W)|FD-Score:-6.32|P-value:1.27E-10||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIF1(YBR275C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL27A(YHR010W)|FD-Score:4.45|P-value:4.34E-6||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:4.32|P-value:7.65E-6||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS12(YOR369C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RPS8A(YBL072C)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RUP1(YOR138C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SAP185(YJL098W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGE1(YPR198W)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHC1(YER096W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SHU1(YHL006C)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIT1(YEL065W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p Gene:SIT4(YDL047W)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLT2(YHR030C)|FD-Score:-4.09|P-value:2.19E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF2(YOR290C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPI1(YER150W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO1(YNL012W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPS100(YHR139C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SPT23(YKL020C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SSN3(YPL042C)|FD-Score:-4.4|P-value:5.39E-6||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STE50(YCL032W)|FD-Score:4.4|P-value:5.40E-6||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUR4(YLR372W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC7(YLR385C)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:SWF1(YDR126W)|FD-Score:-5.76|P-value:4.22E-9||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SYF2(YGR129W)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TEC1(YBR083W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member Gene:TGS1(YPL157W)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOF2(YKR010C)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS65(YGR166W)|FD-Score:-4.94|P-value:3.82E-7||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:VAC8(YEL013W)|FD-Score:7.87|P-value:1.79E-15||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VID28(YIL017C)|FD-Score:-3.86|P-value:5.67E-5||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:WTM1(YOR230W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBL055C(YBL055C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR141C(YBR141C_p)|FD-Score:-5.18|P-value:1.12E-7||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCL046W(YCL046W_d)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCP4(YCR004C)|FD-Score:6.32|P-value:1.32E-10||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YEL1(YBL060W)|FD-Score:5.05|P-value:2.26E-7||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YGL260W(YGL260W_p)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR064W(YGR064W_d)|FD-Score:5.23|P-value:8.58E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR107W(YGR107W_d)|FD-Score:-4.44|P-value:4.50E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHB1(YGR234W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YHC3(YJL059W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YKL027W(YKL027W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL070W(YKL070W_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR202C(YLR202C_d)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR297W(YLR297W_p)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:6.63|P-value:1.71E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR248W(YOR248W_d)|FD-Score:6.13|P-value:4.48E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL205C(YPL205C_d)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPS1(YLR120C)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT6(YLR262C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL164C5.561.33E-80.90CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YBR198C5.229.02E-80.90TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YIL068C5.081.87E-70.90SEC6Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
YDL153C4.181.45E-50.01SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YFL039C4.171.54E-50.06ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YGR114C_d4.102.04E-50.16YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YML085C3.953.96E-50.04TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YDL092W3.914.62E-50.21SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
YOL022C3.701.08E-40.02TSR4Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
YPR142C_d3.681.18E-40.20YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YBR087W3.482.52E-40.03RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YMR168C3.452.81E-40.08CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YAR008W3.373.78E-40.01SEN34Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
YDL209C3.363.96E-40.01CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YDR168W3.344.13E-40.10CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR176C12.801.55E-37RFX1Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins
YGL234W8.662.44E-18ADE5,7Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
YEL013W7.871.79E-15VAC8Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions
YOR071C7.106.22E-13NRT1High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity
YLR110C7.001.29E-12CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YML122C_d6.631.71E-11YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YIR023W6.523.53E-11DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YCR004C6.321.32E-10YCP4Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YKR003W6.252.11E-10OSH6Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion
YOR248W_d6.134.48E-10YOR248W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YFL011W5.783.66E-9HXT10Putative hexose transporter, expressed at low levels and expression is repressed by glucose
YDR245W5.754.49E-9MNN10Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
YOR051C5.422.96E-8ETT1Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
YGR064W_d5.238.58E-8YGR064W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C
YBL060W5.052.26E-7YEL1Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip

GO enrichment analysis for SGTC_2594
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1254.11E-22SGTC_2602kawain 100.0 μMMicrosource (Natural product library)53691290.149254
0.1202.37E-20SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0724638
0.1194.64E-20SGTC_31259127534 49.5 μMChembridge (Drug-like library)16392350.115942
0.1142.22E-18SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0615385
0.1062.96E-16SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.101266
0.1031.70E-15SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0153846
0.1001.42E-14SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.132353
0.1001.69E-14SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.111111
0.0985.62E-14SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.0289855
0.0961.37E-13SGTC_32849108171 64.9 μMChembridge (Drug-like library)86875050.153846
0.0943.45E-13SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.126761amide catabolism
0.0945.48E-13SGTC_10563448-1962 115.0 μMChemDiv (Drug-like library)7155070.119403amide catabolism
0.0936.35E-13SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.161765
0.0921.16E-12SGTC_24605552655 174.2 μMMiscellaneous54019890.0694444amide catabolism
0.0921.83E-12SGTC_29769009005 19.5 μMChembridge (Drug-like library)29931480.0789474

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1123mangostin811.29 nM0.6153855281650TimTec (Natural product library)410.459645.93536mitochondrial response to ROS
SGTC_25903,4',5',6',7'-pentamethoxyflavone100 μM0.37931521171Microsource (Natural product library)372.36862.7580760S ribosome export
SGTC_2595hexamethylquercetagetin100 μM0.37931386331Microsource (Natural product library)402.394582.74208
SGTC_919tnp00227403.57 nM0.3751269246TimTec (Natural product library)458.414782.31010RNA pol III & RNase P/MRP
SGTC_1852st05625431.4 μM0.344828442583TimTec (Natural product derivative library)312.316643.08705
SGTC_2596sinensetin100 μM0.338983145659Microsource (Natural product library)372.36863.05407
SGTC_2518prenyletin46.28 μM0.3114753873459Microsource (Natural product library)246.258563.10614
SGTC_1548st06146890.8 μM0.285714390799TimTec (Pure natural product library)220.221282.31304
SGTC_15666,7-dimethoxy-4-ethylcoumarin85.4 μM0.285714688761TimTec (Pure natural product library)234.247862.76904
SGTC_2620irigenin100 μM0.2857145464170Microsource (Natural product library)360.314842.09138