0302-0163

2-(2,4-dinitroanilino)phenol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_26
Screen concentration 425.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 3100915
SMILES C1=CC=C(C(=C1)NC2=C(C=C(C=C2)[N+](=O)[O-])[N+](=O)[O-])O
Standardized SMILES Oc1ccccc1Nc2ccc(cc2[N+](=O)[O-])[N+](=O)[O-]
Molecular weight 275.217
ALogP 2.93
H-bond donor count 2
H-bond acceptor count 6
Response signature heme biosynthesis & mitochondrial translocase

Pool Growth Kinetics
% growth inhibition (Het. pool) 43.56
% growth inhibition (Hom. pool) 13.38


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3100915
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:3.9|P-value:4.91E-5|Clearance:0.06||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.83|P-value:6.33E-5|Clearance:0.16||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT8(YJL008C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC48(YDL126C)|FD-Score:4.48|P-value:3.76E-6|Clearance:0.03||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:HEM12(YDR047W)|FD-Score:8.66|P-value:2.37E-18|Clearance:2.74||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HYP2(YEL034W)|FD-Score:5.92|P-value:1.64E-9|Clearance:0.03||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:NOP15(YNL110C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.22||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RPC53(YDL150W)|FD-Score:3.99|P-value:3.36E-5|Clearance:0.03||SGD DESC:RNA polymerase III subunit C53 Gene:RPN5(YDL147W)|FD-Score:3.96|P-value:3.79E-5|Clearance:0.06||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RRP45(YDR280W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RSC8(YFR037C)|FD-Score:3.23|P-value:6.30E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.25|P-value:7.76E-8|Clearance:0.77||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SLD3(YGL113W)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.01||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUI2(YJR007W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.05||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF9(YMR236W)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.04||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:UTP21(YLR409C)|FD-Score:3.14|P-value:8.37E-4|Clearance:0.01||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:UTP25(YIL091C)|FD-Score:4.45|P-value:4.33E-6|Clearance:0.46||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YBL073W(YBL073W_d)|FD-Score:-3.17|P-value:7.54E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:YGR114C(YGR114C_d)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YLR198C(YLR198C_d)|FD-Score:5.88|P-value:1.99E-9|Clearance:0.44||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YMR290W-A(YMR290W-A_d)|FD-Score:5.45|P-value:2.53E-8|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:CCT2(YIL142W)|FD-Score:3.9|P-value:4.91E-5|Clearance:0.06||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.83|P-value:6.33E-5|Clearance:0.16||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT8(YJL008C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC48(YDL126C)|FD-Score:4.48|P-value:3.76E-6|Clearance:0.03||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:HEM12(YDR047W)|FD-Score:8.66|P-value:2.37E-18|Clearance:2.74||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HYP2(YEL034W)|FD-Score:5.92|P-value:1.64E-9|Clearance:0.03||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:NOP15(YNL110C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.22||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RPC53(YDL150W)|FD-Score:3.99|P-value:3.36E-5|Clearance:0.03||SGD DESC:RNA polymerase III subunit C53 Gene:RPN5(YDL147W)|FD-Score:3.96|P-value:3.79E-5|Clearance:0.06||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RRP45(YDR280W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RSC8(YFR037C)|FD-Score:3.23|P-value:6.30E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.25|P-value:7.76E-8|Clearance:0.77||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SLD3(YGL113W)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.01||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUI2(YJR007W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.05||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF9(YMR236W)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.04||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:UTP21(YLR409C)|FD-Score:3.14|P-value:8.37E-4|Clearance:0.01||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:UTP25(YIL091C)|FD-Score:4.45|P-value:4.33E-6|Clearance:0.46||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YBL073W(YBL073W_d)|FD-Score:-3.17|P-value:7.54E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:YGR114C(YGR114C_d)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YLR198C(YLR198C_d)|FD-Score:5.88|P-value:1.99E-9|Clearance:0.44||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YMR290W-A(YMR290W-A_d)|FD-Score:5.45|P-value:2.53E-8|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3100915
Download HOP data (tab-delimited text)  (excel)
Gene:ALP1(YNL270C)|FD-Score:5.32|P-value:5.16E-8||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CBT1(YKL208W)|FD-Score:-4.44|P-value:4.56E-6||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CNM67(YNL225C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COX9(YDL067C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTI6(YPL181W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CYS3(YAL012W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL7(YIR031C)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DSS1(YMR287C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM33(YBR078W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FAA1(YOR317W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FKS1(YLR342W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:GMC1(YDR506C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GSP2(YOR185C)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GUF1(YLR289W)|FD-Score:-4.82|P-value:7.02E-7||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HEM14(YER014W)|FD-Score:3.79|P-value:7.47E-5||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:IVY1(YDR229W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:JEN1(YKL217W)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MGR2(YPL098C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MRP51(YPL118W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:NVJ1(YHR195W)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:RDL2(YOR286W)|FD-Score:-6.88|P-value:2.97E-12||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RIM1(YCR028C-A)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPL24A(YGL031C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS29A(YLR388W)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SFL1(YOR140W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SNF7(YLR025W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPT10(YJL127C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SSE1(YPL106C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSF1(YHR066W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SVP26(YHR181W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SYF2(YGR129W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TOM70(YNL121C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:YMR244W(YMR244W_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function Gene:YNR048W(YNR048W)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOL131W(YOL131W_p)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Putative protein of unknown function Gene:ALP1(YNL270C)|FD-Score:5.32|P-value:5.16E-8||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CBT1(YKL208W)|FD-Score:-4.44|P-value:4.56E-6||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CNM67(YNL225C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COX9(YDL067C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTI6(YPL181W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CYS3(YAL012W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL7(YIR031C)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DSS1(YMR287C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM33(YBR078W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FAA1(YOR317W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FKS1(YLR342W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:GMC1(YDR506C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GSP2(YOR185C)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GUF1(YLR289W)|FD-Score:-4.82|P-value:7.02E-7||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HEM14(YER014W)|FD-Score:3.79|P-value:7.47E-5||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:IVY1(YDR229W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:JEN1(YKL217W)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MGR2(YPL098C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MRP51(YPL118W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:NVJ1(YHR195W)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:RDL2(YOR286W)|FD-Score:-6.88|P-value:2.97E-12||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RIM1(YCR028C-A)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPL24A(YGL031C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS29A(YLR388W)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SFL1(YOR140W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SNF7(YLR025W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPT10(YJL127C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SSE1(YPL106C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSF1(YHR066W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SVP26(YHR181W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SYF2(YGR129W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TOM70(YNL121C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:YMR244W(YMR244W_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function Gene:YNR048W(YNR048W)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOL131W(YOL131W_p)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR047W8.662.37E-182.74HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YEL034W5.921.64E-90.03HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YLR198C_d5.881.99E-90.44YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YMR290W-A_d5.452.53E-80.20YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YML127W5.257.76E-80.77RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YDL126C4.483.76E-60.03CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YIL091C4.454.33E-60.46UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YDL150W3.993.36E-50.03RPC53RNA polymerase III subunit C53
YDL147W3.963.79E-50.06RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YIL142W3.904.91E-50.06CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJR064W3.836.33E-50.16CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJR007W3.671.22E-40.05SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YMR236W3.611.51E-40.04TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YJL014W3.571.78E-40.13CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YNL110C3.442.89E-40.22NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL270C5.325.16E-8ALP1Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication
YPL069C4.701.28E-6BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YPL098C4.121.93E-5MGR2Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype)
YPL193W4.072.39E-5RSA1Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
YBR078W3.924.42E-5ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YLR388W3.855.84E-5RPS29AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication
YPL106C3.836.35E-5SSE1ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication
YIR031C3.797.45E-5DAL7Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YER014W3.797.47E-5HEM14Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides
YHR195W3.729.84E-5NVJ1Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
YDL067C3.661.27E-4COX9Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YPL118W3.631.44E-4MRP51Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
YPL181W3.601.57E-4CTI6Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
YML073C3.601.60E-4RPL6ARibosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
YMR244W_p3.581.69E-4YMR244W_pPutative protein of unknown function

GO enrichment analysis for SGTC_26
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2103.33E-59SGTC_10258-0098 57.4 μMChemDiv (Drug-like library)67900320.333333heme biosynthesis & mitochondrial translocase
0.2002.79E-54SGTC_273hydroxyurea 18.1 mMMiscellaneous36570.0731707heme biosynthesis & mitochondrial translocase
0.1831.94E-45SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.0921053heme biosynthesis & mitochondrial translocase
0.1799.86E-44SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0289855heme biosynthesis & mitochondrial translocase
0.1605.16E-35SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.0892857heme biosynthesis & mitochondrial translocase
0.1211.13E-20SGTC_690096-0274 206.7 μMChemDiv (Drug-like library)67413920.0793651mitochondrial processes
0.1211.17E-20SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.122449
0.1194.34E-20SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.0746269copper-dependent oxidative stress
0.1162.92E-19SGTC_203k066-0004 30.9 μMChemDiv (Drug-like library)67954120.0882353RSC & ERG11
0.1132.52E-18SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0555556heme biosynthesis & mitochondrial translocase
0.1134.28E-18SGTC_1769st044406 64.4 μMTimTec (Natural product derivative library)6709030.0724638
0.1111.71E-17SGTC_553k007-0256 45.7 μMChemDiv (Drug-like library)62870000.0677966heme biosynthesis & mitochondrial translocase
0.1071.49E-16SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.0877193heme biosynthesis & mitochondrial translocase
0.1063.36E-16SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.111111heme biosynthesis & mitochondrial translocase
0.1031.79E-15SGTC_11564092-0367 4.9 μMChemDiv (Drug-like library)13575850.102941

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_410478-063282.64 μM0.4545453750424ChemDiv (Drug-like library)229.234642.52724
SGTC_230213-001140 μM0.452381627608ChemDiv (Drug-like library)304.215123.06317copper-dependent oxidative stress
SGTC_8890919-152482.7 μM0.450983905452ChemDiv (Drug-like library)274.271962.85825
SGTC_1764st04450293.5 μM0.458407TimTec (Natural product derivative library)214.223.27413
SGTC_4260988-003714.6 μM0.45383008ChemDiv (Drug-like library)257.244741.51425ERAD & cell cycle
SGTC_225nsc-1226571.02 μM0.395833275685Miscellaneous322.29332.72806
SGTC_6260214-000933 μM0.3877553437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress
SGTC_3710195-00067.36 μM0.3809523928593ChemDiv (Drug-like library)253.209682.70204
SGTC_7930488-0545106 μM0.377778292548ChemDiv (Drug-like library)237.212042.24105
SGTC_8201582-006059.9 μM0.3725493848840ChemDiv (Drug-like library)290.661983.50816