thymoquinone

2-methyl-5-propan-2-ylcyclohexa-2,5-diene-1,4-dione

A benzoquinone with antoxidant properties and demonstrated potential to act as a histone deacetylase inhibitor (HDAC).

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2608
Screen concentration 42.7 μM
Source Microsource (Natural product library)
PubChem CID 10281
SMILES CC1=CC(=O)C(=CC1=O)C(C)C
Standardized SMILES CC(C)C1=CC(=O)C(=CC1=O)C
Molecular weight 164.2011
ALogP 2.29
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.2
% growth inhibition (Hom. pool) 11.04


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 10281
Download HIP data (tab-delimited text)  (excel)
Gene:ALG13(YGL047W)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.22||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:CAB3(YKL088W)|FD-Score:-3.11|P-value:9.40E-4|Clearance:0||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CIA1(YDR267C)|FD-Score:-4.15|P-value:1.70E-5|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:DIB1(YPR082C)|FD-Score:-3.79|P-value:7.59E-5|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:FBA1(YKL060C)|FD-Score:3.58|P-value:1.73E-4|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:HEM15(YOR176W)|FD-Score:-3.17|P-value:7.61E-4|Clearance:0||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:IPI1(YHR085W)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.08||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:MRD1(YPR112C)|FD-Score:4.94|P-value:3.88E-7|Clearance:1.27||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NNF1(YJR112W)|FD-Score:3.68|P-value:1.19E-4|Clearance:0.06||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NOP56(YLR197W)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.02||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:POP4(YBR257W)|FD-Score:-4.89|P-value:5.05E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:QRI1(YDL103C)|FD-Score:6.34|P-value:1.12E-10|Clearance:1.4||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RPC19(YNL113W)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPC31(YNL151C)|FD-Score:-3.95|P-value:3.83E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RSA4(YCR072C)|FD-Score:3.2|P-value:6.93E-4|Clearance:0.04||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SNU13(YEL026W)|FD-Score:3.3|P-value:4.84E-4|Clearance:0.1||SGD DESC:RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein Gene:TAF4(YMR005W)|FD-Score:-3.46|P-value:2.68E-4|Clearance:0||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TAF5(YBR198C)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.04||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF6(YGL112C)|FD-Score:-5.2|P-value:9.92E-8|Clearance:0||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:YDR355C(YDR355C_d)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.04||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:ALG13(YGL047W)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.22||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:CAB3(YKL088W)|FD-Score:-3.11|P-value:9.40E-4|Clearance:0||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CIA1(YDR267C)|FD-Score:-4.15|P-value:1.70E-5|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:DIB1(YPR082C)|FD-Score:-3.79|P-value:7.59E-5|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:FBA1(YKL060C)|FD-Score:3.58|P-value:1.73E-4|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:HEM15(YOR176W)|FD-Score:-3.17|P-value:7.61E-4|Clearance:0||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:IPI1(YHR085W)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.08||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:MRD1(YPR112C)|FD-Score:4.94|P-value:3.88E-7|Clearance:1.27||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NNF1(YJR112W)|FD-Score:3.68|P-value:1.19E-4|Clearance:0.06||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NOP56(YLR197W)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.02||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:POP4(YBR257W)|FD-Score:-4.89|P-value:5.05E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:QRI1(YDL103C)|FD-Score:6.34|P-value:1.12E-10|Clearance:1.4||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RPC19(YNL113W)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPC31(YNL151C)|FD-Score:-3.95|P-value:3.83E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RSA4(YCR072C)|FD-Score:3.2|P-value:6.93E-4|Clearance:0.04||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SNU13(YEL026W)|FD-Score:3.3|P-value:4.84E-4|Clearance:0.1||SGD DESC:RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein Gene:TAF4(YMR005W)|FD-Score:-3.46|P-value:2.68E-4|Clearance:0||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TAF5(YBR198C)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.04||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF6(YGL112C)|FD-Score:-5.2|P-value:9.92E-8|Clearance:0||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:YDR355C(YDR355C_d)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.04||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 10281
Download HOP data (tab-delimited text)  (excel)
Gene:ACH1(YBL015W)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:AKR2(YOR034C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:ATP19(YOL077W-A)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:CBP2(YHL038C)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:CLB1(YGR108W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLG1(YGL215W)|FD-Score:-3.31|P-value:4.58E-4||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:DAK1(YML070W)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DEG1(YFL001W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:EAF6(YJR082C)|FD-Score:6.44|P-value:6.01E-11||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM18(YDR125C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FMT1(YBL013W)|FD-Score:-4.66|P-value:1.60E-6||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FOB1(YDR110W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FPR1(YNL135C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GIM4(YEL003W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GLE2(YER107C)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GRE1(YPL223C)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:GRE2(YOL151W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HST4(YDR191W)|FD-Score:8.98|P-value:1.34E-19||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:JNM1(YMR294W)|FD-Score:-3.8|P-value:7.24E-5||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LRS4(YDR439W)|FD-Score:3.73|P-value:9.41E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAL32(YBR299W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MAM33(YIL070C)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MDH1(YKL085W)|FD-Score:-5|P-value:2.94E-7||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MMT2(YPL224C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MNR2(YKL064W)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MRS1(YIR021W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:MSB4(YOL112W)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSS11(YMR164C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NMD2(YHR077C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NRK1(YNL129W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis Gene:OPI9(YLR338W_d)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PHO85(YPL031C)|FD-Score:-4.04|P-value:2.62E-5||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PLB3(YOL011W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PST2(YDR032C)|FD-Score:6.5|P-value:4.03E-11||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC4(YBR125C)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RRD2(YPL152W)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRT14(YIL127C_p)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:SAS2(YMR127C)|FD-Score:5.3|P-value:5.78E-8||SGD DESC:Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family Gene:SET4(YJL105W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SGF29(YCL010C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SHO1(YER118C)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SNZ2(YNL333W)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPA2(YLL021W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPE2(YOL052C)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPO16(YHR153C)|FD-Score:9.07|P-value:5.77E-20||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SSO2(YMR183C)|FD-Score:-3.72|P-value:9.84E-5||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:STE13(YOR219C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:TIF2(YJL138C)|FD-Score:4.27|P-value:9.93E-6||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TOM1(YDR457W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRM1(YDR120C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:UBC4(YBR082C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBP14(YBR058C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UIP4(YPL186C)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VBA1(YMR088C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VHS3(YOR054C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:YAR1(YPL239W)|FD-Score:4.34|P-value:7.23E-6||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR013C(YBR013C_p)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YDR095C(YDR095C_d)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR215C(YDR215C_d)|FD-Score:-5.09|P-value:1.83E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER066C-A(YER066C-A_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL199C(YGL199C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR093W(YGR093W_p)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YHR125W(YHR125W_d)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR130C(YHR130C_d)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL108W(YIL108W_p)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIL166C(YIL166C_p)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJR038C(YJR038C_d)|FD-Score:4.31|P-value:8.32E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR129C(YJR129C_p)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL075C(YKL075C_p)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YNL011C(YNL011C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YNL295W(YNL295W_p)|FD-Score:5.34|P-value:4.71E-8||SGD DESC:Putative protein of unknown function Gene:YPL185W(YPL185W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPL197C(YPL197C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPL205C(YPL205C_d)|FD-Score:8.16|P-value:1.64E-16||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:ACH1(YBL015W)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:AKR2(YOR034C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:ATP19(YOL077W-A)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:CBP2(YHL038C)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:CLB1(YGR108W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLG1(YGL215W)|FD-Score:-3.31|P-value:4.58E-4||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:DAK1(YML070W)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DEG1(YFL001W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:EAF6(YJR082C)|FD-Score:6.44|P-value:6.01E-11||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM18(YDR125C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FMT1(YBL013W)|FD-Score:-4.66|P-value:1.60E-6||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FOB1(YDR110W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FPR1(YNL135C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GIM4(YEL003W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GLE2(YER107C)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GRE1(YPL223C)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:GRE2(YOL151W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HST4(YDR191W)|FD-Score:8.98|P-value:1.34E-19||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:JNM1(YMR294W)|FD-Score:-3.8|P-value:7.24E-5||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LRS4(YDR439W)|FD-Score:3.73|P-value:9.41E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAL32(YBR299W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MAM33(YIL070C)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MDH1(YKL085W)|FD-Score:-5|P-value:2.94E-7||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MMT2(YPL224C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MNR2(YKL064W)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MRS1(YIR021W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:MSB4(YOL112W)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSS11(YMR164C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NMD2(YHR077C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NRK1(YNL129W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis Gene:OPI9(YLR338W_d)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PHO85(YPL031C)|FD-Score:-4.04|P-value:2.62E-5||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PLB3(YOL011W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PST2(YDR032C)|FD-Score:6.5|P-value:4.03E-11||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC4(YBR125C)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RRD2(YPL152W)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRT14(YIL127C_p)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:SAS2(YMR127C)|FD-Score:5.3|P-value:5.78E-8||SGD DESC:Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family Gene:SET4(YJL105W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SGF29(YCL010C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SHO1(YER118C)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SNZ2(YNL333W)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPA2(YLL021W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPE2(YOL052C)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPO16(YHR153C)|FD-Score:9.07|P-value:5.77E-20||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SSO2(YMR183C)|FD-Score:-3.72|P-value:9.84E-5||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:STE13(YOR219C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:TIF2(YJL138C)|FD-Score:4.27|P-value:9.93E-6||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TOM1(YDR457W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRM1(YDR120C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:UBC4(YBR082C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBP14(YBR058C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UIP4(YPL186C)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VBA1(YMR088C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VHS3(YOR054C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:YAR1(YPL239W)|FD-Score:4.34|P-value:7.23E-6||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR013C(YBR013C_p)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YDR095C(YDR095C_d)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR215C(YDR215C_d)|FD-Score:-5.09|P-value:1.83E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER066C-A(YER066C-A_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL199C(YGL199C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR093W(YGR093W_p)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YHR125W(YHR125W_d)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR130C(YHR130C_d)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL108W(YIL108W_p)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIL166C(YIL166C_p)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJR038C(YJR038C_d)|FD-Score:4.31|P-value:8.32E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR129C(YJR129C_p)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL075C(YKL075C_p)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YNL011C(YNL011C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YNL295W(YNL295W_p)|FD-Score:5.34|P-value:4.71E-8||SGD DESC:Putative protein of unknown function Gene:YPL185W(YPL185W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPL197C(YPL197C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPL205C(YPL205C_d)|FD-Score:8.16|P-value:1.64E-16||SGD DESC:Hypothetical protein; deletion of locus affects telomere length

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL103C6.341.12E-101.40QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YPR112C4.943.88E-71.27MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YJR112W3.681.19E-40.06NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YDR355C_d3.621.49E-40.04YDR355C_dDubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
YKL060C3.581.73E-49.98E-4FBA1Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress
YGL047W3.581.74E-40.21ALG13Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
YLR197W3.363.87E-40.02NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YBR198C3.344.15E-40.04TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YEL026W3.304.84E-40.10SNU13RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein
YCR072C3.206.93E-40.04RSA4WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
YHR085W3.158.04E-40.08IPI1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
YOR287C3.070.001060.19RRP36Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
YGL074C_d2.880.001960.03YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YNR016C2.850.002170.01ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YNR038W2.840.002270.03DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR153C9.075.77E-20SPO16Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
YDR191W8.981.34E-19HST4Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
YPL205C_d8.161.64E-16YPL205C_dHypothetical protein; deletion of locus affects telomere length
YDR032C6.504.03E-11PST2Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
YJR082C6.446.01E-11EAF6Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3
YNL295W_p5.344.71E-8YNL295W_pPutative protein of unknown function
YMR127C5.305.78E-8SAS2Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
YBR125C5.072.00E-7PTC4Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
YLL021W4.395.70E-6SPA2Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
YPL239W4.347.23E-6YAR1Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock
YJR038C_d4.318.32E-6YJR038C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJL138C4.279.93E-6TIF2Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
YHR077C4.211.26E-5NMD2Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance
YBR058C4.102.07E-5UBP14Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T
YER107C4.082.28E-5GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1

GO enrichment analysis for SGTC_2608
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2654.29E-95SGTC_2605digitonin 300.0 nMTimTec (Pure natural product library)27350100.00952381
0.1271.47E-22SGTC_200481-0013 4.9 μMChemDiv (Drug-like library)1820450.102564
0.0903.94E-12SGTC_2616abietic acid 100.0 μMMicrosource (Natural product library)67086780.140351
0.0863.71E-11SGTC_2693st077581 40.7 μMTimTec (Natural product derivative library)252387610.0722892
0.0766.19E-9SGTC_12460422-0085 105.0 μMChemDiv (Drug-like library)2642950.102564
0.0757.14E-9SGTC_21035378138 10.0 μMChembridge (Fragment library)2441420.0952381
0.0723.21E-8SGTC_2602kawain 100.0 μMMicrosource (Natural product library)53691290.12
0.0552.32E-5SGTC_8570438-0306 1.4 μMChemDiv (Drug-like library)46861360.147059copper-dependent oxidative stress
0.0534.30E-5SGTC_24585808907 16.4 μMMiscellaneous27317470.0677966
0.0517.65E-5SGTC_12201838-0075 193.0 μMChemDiv (Drug-like library)55296450.0666667
0.0519.63E-5SGTC_28409008771 71.4 μMChembridge (Drug-like library)29929780.046875
0.0482.34E-4SGTC_2754granisetron 64.0 μMMiscellaneous52845660.0655738
0.0482.34E-4SGTC_32519135047 49.5 μMChembridge (Drug-like library)176829630.080645260S ribosome export
0.0473.50E-4SGTC_20525265182 7.1 μMChembridge (Fragment library)12398840.0909091
0.0456.15E-4SGTC_1779st039427 55.0 μMTimTec (Natural product derivative library)11244980.0806452

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5481436-0017110 μM0.3333339677137ChemDiv (Drug-like library)328.319343.73806TSC3-RPN4
SGTC_2681menadione3.18 μM0.3225814055Miscellaneous172.180022.20402superoxide
SGTC_394menadione8.33 μM0.3225814055Miscellaneous172.180022.20402superoxide
SGTC_1556plumbagin1.48 μM0.2702710205TimTec (Pure natural product library)188.179421.96213superoxide
SGTC_2575plumbagin510 nM0.2702710205TimTec (Pure natural product library)188.179421.96213superoxide
SGTC_25354',4'-dimethoxydalbergione51.19 μM0.25364106Microsource (Natural product library)284.306542.61104DNA damage response
SGTC_1110083-003952.46 μM0.222222759313ChemDiv (Drug-like library)238.281163.55902
SGTC_26133',4'-dimethoxydalbergione24.65 μM0.222222115019Microsource (Natural product library)284.306542.31504
SGTC_1686st019653115.5 μM0.21621697252TimTec (Natural product derivative library)173.211141.90401mitochondrial processes
SGTC_2242663044961.27 μM0.214286691249Chembridge (Fragment library)210.1768262.55204Golgi
SGTC_21715739187198.08 μM0.2121212262761Chembridge (Fragment library)222.283520.90324copper-dependent oxidative stress
SGTC_25534-methylesculetin85.09 μM0.2105265319502Microsource (Natural product library)192.168121.86224