5-fluorouridine

1-[3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-fluoropyrimidine-2,4-dione

5-fluorouridine (FUrd) is FDA-approved anticancer drug. As a 5-fluorauracil metabolite, FUrd is thought to also act by inhibiting thymidylate synthetase and through metabolism into cytotoxic ribonucleotides and deoxyribonucleotides that can be incorporated into DNA and RNA. In addition to being incorporated in DNA and RNA, the drug has been shown to inhibit the essential ribonuclease activity of the exosome complex.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_261
Screen concentration 15.4 μM
Source Miscellaneous
PubChem CID 1821
SMILES C1=C(C(=O)NC(=O)N1C2C(C(C(O2)CO)O)O)F
Standardized SMILES OCC1OC(C(O)C1O)N2C=C(F)C(=O)NC2=O
Molecular weight 262.1918
ALogP -2.54
H-bond donor count 4
H-bond acceptor count 7
Response signature exosome

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.54
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1821
Download HIP data (tab-delimited text)  (excel)
Gene:CDC31(YOR257W)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DIS3(YOL021C)|FD-Score:11.5|P-value:1.16E-30|Clearance:4.6||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:EBP2(YKL172W)|FD-Score:5.05|P-value:2.22E-7|Clearance:0.37||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:GPI8(YDR331W)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.17||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:GRC3(YLL035W)|FD-Score:-4.09|P-value:2.16E-5|Clearance:0||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:IPL1(YPL209C)|FD-Score:3.89|P-value:5.10E-5|Clearance:0.19||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:MTR3(YGR158C)|FD-Score:4.33|P-value:7.41E-6|Clearance:0.28||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOP1(YDL014W)|FD-Score:3.91|P-value:4.58E-5|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NOP2(YNL061W)|FD-Score:4.47|P-value:3.91E-6|Clearance:0.14||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP4(YPL043W)|FD-Score:3.12|P-value:8.93E-4|Clearance:0.13||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:PRP24(YMR268C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.17||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PXR1(YGR280C)|FD-Score:3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain Gene:RLP24(YLR009W)|FD-Score:4.56|P-value:2.60E-6|Clearance:0.04||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RLP7(YNL002C)|FD-Score:3.35|P-value:4.05E-4|Clearance:0.22||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RRP15(YPR143W)|FD-Score:5.2|P-value:1.00E-7|Clearance:0.15||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP4(YHR069C)|FD-Score:4.05|P-value:2.59E-5|Clearance:0.1||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP42(YDL111C)|FD-Score:6.85|P-value:3.59E-12|Clearance:1.22||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:12|P-value:2.10E-33|Clearance:4.6||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.12||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RVB2(YPL235W)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.04||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SKI6(YGR195W)|FD-Score:5.63|P-value:8.88E-9|Clearance:0.43||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:TAD2(YJL035C)|FD-Score:-3.88|P-value:5.25E-5|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:YNL247W(YNL247W)|FD-Score:4.52|P-value:3.11E-6|Clearance:0.05||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:CDC31(YOR257W)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DIS3(YOL021C)|FD-Score:11.5|P-value:1.16E-30|Clearance:4.6||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:EBP2(YKL172W)|FD-Score:5.05|P-value:2.22E-7|Clearance:0.37||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:GPI8(YDR331W)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.17||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:GRC3(YLL035W)|FD-Score:-4.09|P-value:2.16E-5|Clearance:0||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:IPL1(YPL209C)|FD-Score:3.89|P-value:5.10E-5|Clearance:0.19||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:MTR3(YGR158C)|FD-Score:4.33|P-value:7.41E-6|Clearance:0.28||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOP1(YDL014W)|FD-Score:3.91|P-value:4.58E-5|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NOP2(YNL061W)|FD-Score:4.47|P-value:3.91E-6|Clearance:0.14||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP4(YPL043W)|FD-Score:3.12|P-value:8.93E-4|Clearance:0.13||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:PRP24(YMR268C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.17||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PXR1(YGR280C)|FD-Score:3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain Gene:RLP24(YLR009W)|FD-Score:4.56|P-value:2.60E-6|Clearance:0.04||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RLP7(YNL002C)|FD-Score:3.35|P-value:4.05E-4|Clearance:0.22||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RRP15(YPR143W)|FD-Score:5.2|P-value:1.00E-7|Clearance:0.15||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP4(YHR069C)|FD-Score:4.05|P-value:2.59E-5|Clearance:0.1||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP42(YDL111C)|FD-Score:6.85|P-value:3.59E-12|Clearance:1.22||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:12|P-value:2.10E-33|Clearance:4.6||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.12||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RVB2(YPL235W)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.04||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SKI6(YGR195W)|FD-Score:5.63|P-value:8.88E-9|Clearance:0.43||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:TAD2(YJL035C)|FD-Score:-3.88|P-value:5.25E-5|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:YNL247W(YNL247W)|FD-Score:4.52|P-value:3.11E-6|Clearance:0.05||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1821
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:APE3(YBR286W)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:AZR1(YGR224W)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BAP2(YBR068C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BNA4(YBL098W)|FD-Score:-3.73|P-value:9.50E-5||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CCC2(YDR270W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CHK1(YBR274W)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase Gene:COG6(YNL041C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR7(YJR032W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CSG2(YBR036C)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTF8(YHR191C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAT1(YML113W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DMA2(YNL116W)|FD-Score:6.1|P-value:5.27E-10||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DPB3(YBR278W)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ECL1(YGR146C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EUG1(YDR518W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:FAR11(YNL127W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FLO10(YKR102W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth Gene:FUI1(YBL042C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FUN14(YAL008W)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Mitochondrial protein of unknown function Gene:HHF1(YBR009C)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HPC2(YBR215W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:HST3(YOR025W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:ICT1(YLR099C)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:INM1(YHR046C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:KIN2(YLR096W)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KTI12(YKL110C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MAC1(YMR021C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDS3(YGL197W)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MRM1(YOR201C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MSH2(YOL090W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NAT5(YOR253W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:PAH1(YMR165C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PCI8(YIL071C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PET112(YBL080C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX18(YHR160C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX30(YLR324W)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PRS2(YER099C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:RIM4(YHL024W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RSA3(YLR221C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SFA1(YDL168W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SIP5(YMR140W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:STE23(YLR389C)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STE50(YCL032W)|FD-Score:6.75|P-value:7.58E-12||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:VPS27(YNR006W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YBR051W(YBR051W_d)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR197C(YBR197C_p)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCR102C(YCR102C_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YER034W(YER034W_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGR237C(YGR237C_p)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIL165C(YIL165C_p)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YJL047C-A(YJL047C-A_p)|FD-Score:4.29|P-value:8.95E-6||SGD DESC:Putative protein of unknown function Gene:YJL136W-A(YJL136W-A_p)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YLR053C(YLR053C_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function Gene:YLR224W(YLR224W_p)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:-4.94|P-value:3.95E-7||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YNL205C(YNL205C_d)|FD-Score:6.67|P-value:1.32E-11||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR014C(YPR014C_d)|FD-Score:-3.83|P-value:6.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:ACB1(YGR037C)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:APE3(YBR286W)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:AZR1(YGR224W)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BAP2(YBR068C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BNA4(YBL098W)|FD-Score:-3.73|P-value:9.50E-5||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CCC2(YDR270W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CHK1(YBR274W)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase Gene:COG6(YNL041C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR7(YJR032W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CSG2(YBR036C)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTF8(YHR191C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAT1(YML113W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DMA2(YNL116W)|FD-Score:6.1|P-value:5.27E-10||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DPB3(YBR278W)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ECL1(YGR146C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EUG1(YDR518W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:FAR11(YNL127W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FLO10(YKR102W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth Gene:FUI1(YBL042C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FUN14(YAL008W)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Mitochondrial protein of unknown function Gene:HHF1(YBR009C)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HPC2(YBR215W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:HST3(YOR025W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:ICT1(YLR099C)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:INM1(YHR046C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:KIN2(YLR096W)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KTI12(YKL110C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MAC1(YMR021C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDS3(YGL197W)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MRM1(YOR201C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MSH2(YOL090W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NAT5(YOR253W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:PAH1(YMR165C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PCI8(YIL071C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PET112(YBL080C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX18(YHR160C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX30(YLR324W)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PRS2(YER099C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:RIM4(YHL024W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RSA3(YLR221C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SFA1(YDL168W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SIP5(YMR140W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:STE23(YLR389C)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STE50(YCL032W)|FD-Score:6.75|P-value:7.58E-12||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:VPS27(YNR006W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YBR051W(YBR051W_d)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR197C(YBR197C_p)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCR102C(YCR102C_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YER034W(YER034W_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGR237C(YGR237C_p)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIL165C(YIL165C_p)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YJL047C-A(YJL047C-A_p)|FD-Score:4.29|P-value:8.95E-6||SGD DESC:Putative protein of unknown function Gene:YJL136W-A(YJL136W-A_p)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YLR053C(YLR053C_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function Gene:YLR224W(YLR224W_p)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:-4.94|P-value:3.95E-7||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YNL205C(YNL205C_d)|FD-Score:6.67|P-value:1.32E-11||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR014C(YPR014C_d)|FD-Score:-3.83|P-value:6.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR280W12.002.10E-334.60RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YOL021C11.501.16E-304.60DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YDL111C6.853.59E-121.22RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YGR195W5.638.88E-90.43SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YPR143W5.201.00E-70.15RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YKL172W5.052.22E-70.37EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YGR095C4.681.46E-60.12RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YLR009W4.562.60E-60.04RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YNL247W4.523.11E-60.05YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YNL061W4.473.91E-60.14NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YGR158C4.337.41E-60.28MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YHR069C4.052.59E-50.10RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YPL235W3.953.91E-50.04RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YDL014W3.914.58E-50.03NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YPL209C3.895.10E-50.19IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCL032W6.757.58E-12STE50Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
YNL205C_d6.671.32E-11YNL205C_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL116W6.105.27E-10DMA2Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains
YLR099C5.052.18E-7ICT1Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication
YJL047C-A_p4.298.95E-6YJL047C-A_pPutative protein of unknown function
YJR032W4.121.87E-5CPR7Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity
YBL042C4.062.49E-5FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YOR025W4.003.18E-5HST3Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
YJL136W-A_p3.983.50E-5YJL136W-A_pPutative protein of unknown function; identified by SAGE
YNL127W3.953.93E-5FAR11Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2
YOR201C3.944.10E-5MRM1Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
YBR009C3.904.87E-5HHF1Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
YBR278W3.826.72E-5DPB3Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
YLR324W3.807.26E-5PEX30Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
YLR096W3.797.53E-5KIN2Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p

GO enrichment analysis for SGTC_261
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3551.88E-175SGTC_10775-fluorocytosine 377.0 nMNIH Clinical Collection33660.156863exosome
0.3511.16E-170SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.156863exosome
0.3513.69E-170SGTC_6104215-0184 155.0 μMChemDiv (Drug-like library)56050150.060241exosome
0.3462.54E-165SGTC_1074carmofur 3.8 μMNIH Clinical Collection25770.293103exosome
0.2925.09E-116SGTC_2425hr heat shock (37°C) + 5-fluorouracil 4.6 μMMiscellaneous33850.244444heat shock/prefoldin
0.2579.58E-90SGTC_4235-fluorouracil 457.0 μMMiscellaneous33850.244444exosome
0.2301.43E-71SGTC_6173770-0098 35.0 μMChemDiv (Drug-like library)59626390.0641026exosome
0.1561.43E-33SGTC_33379144342 33.5 μMChembridge (Drug-like library)176829490.0657895
0.1323.12E-24SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.0568182translation
0.1111.62E-17SGTC_2151287-0124 461.5 μMChemDiv (Drug-like library)55608630.0235294
0.1001.89E-14SGTC_3710195-0006 7.4 μMChemDiv (Drug-like library)39285930.0689655
0.0977.30E-14SGTC_32219128209 49.5 μMChembridge (Drug-like library)164607230.0519481
0.0904.96E-12SGTC_22907904846 200.0 μMChembridge (Fragment library)29621540.125
0.0881.37E-11SGTC_274sirtinol 40.1 μMMiscellaneous68364420.05
0.0841.21E-10SGTC_8790831-1030 162.0 μMChemDiv (Drug-like library)15492520.0877193

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_15346-azauridine81.6 μM0.489796233502TimTec (Pure natural product library)245.18944-2.43247RNA pol III & RNase P/MRP
SGTC_1540guanosine70.6 μM0.3278696802TimTec (Pure natural product library)283.24072-2.3856
SGTC_1536cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid61.9 μM0.3174666535TimTec (Pure natural product library)323.196522-2.46858
SGTC_1789tunicamycin25 nM0.29885123847177ICCB bioactive library718.70344-4.5341116unfolded protein response
SGTC_255tunicamycin200.47 nM0.29885123847177ICCB bioactive library718.70344-4.5341116unfolded protein response
SGTC_1528arabinosylhypoxanthine74.6 μM0.295082804TimTec (Pure natural product library)268.22608-2.24946
SGTC_1074carmofur3.8 μM0.2931032577NIH Clinical Collection257.2614431.12524exosome
SGTC_1879gemcitabine100 μM0.27868960750Miscellaneous263.198146-1.39436
SGTC_1956st07708554 μM0.24637724208685TimTec (Natural product derivative library)370.30812-0.531510
SGTC_2425hr heat shock (37°C) + 5-fluorouracil4.57 μM0.2444443385Miscellaneous130.077223-1.10223heat shock/prefoldin
SGTC_4235-fluorouracil457 μM0.2444443385Miscellaneous130.077223-1.10223exosome